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Aktivasi Oosit Menggunakan Strontium Klorida setelah Injeksi dengan Spermatozoa Domba Hasil Pengeringbekuan (OOCYTE ACTIVATION USING STRONTIUM CHLORIDE FOLLOWING INJECTION OF FREEZE-DRIED RAM SPERMATOZOA) Takdir Saili; Ita Djuwita; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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One of the factors that inhibit the formation of male pronuclei following injection of freeze-dried ramspermatozoa was the absence of artificial activation during oocyte incubation after the injection. Therefore,in this experiment the ability of strontium chloride (SrCl2) to improve oocyte activation followingintracytoplasmic sperm injection (ICSI) was evaluated. Aceto lacmoid staining was used to assessdecondensation and pronucleus formation following ICSI. Results of this experiment revealed that freezedriedspermatozoa had the ability to decondense and to form 1PN following injection into oocytes evenwithout artificial activation, but failed to form 2PN. However, 40% of 2PN oocytes were obtained when theinjected oocytes was first incubated for 20 minutes in medium containing 50 mM strontium chloride thensubsequently incubated for 10 hours in medium without strontium. On the contrary, the 2PN oocytes werenot observed either in injected oocyte neither without artificial activation nor in non-injected oocytes withartificial activation. In conclusion, freeze-dried ram spermatozoa were able to decondense and to support2PN formation following ICSI and artificial activation using strontium.

Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup I Wayan Batan; I Ketut Suatha; Ita Djuwita; Nining Handhayani; Wahono Esti Prasetyaningtyas; Ketut Adnyane Mudite; Bibiana W Lay; Supar -; Arief Boediono
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the study was to assess the viability of vitrified embryo using cryoloop as a carrier ofembryo. The blastocyst stage embryos were collected from superovulated mice. Embryos were frozenusing vitrification method and vitrified embryos were loaded on copper filament cryoloop before dipped inliquid nitrogen. The viability of vitrified embryos was assess in vitro by medium cultered and in vivo bytransfered them to recipient mice. The result shows the viability of vitrified embryos was 85,7% after 24hours cultured and the embryos were born from two pregnant recipient mice out of nine (22%) or fouroffspring out of 63 trasfered embryos (6%). In conclusion, vitrified blatocyst stage embryos using cryoloopas a carrier could keep the viability of the embryos and they could be transfered to the recipient mice andwere born normally.

Black Seed (Nigella sativa) Extract Induce in vitro Proliferation and Differentiation of Rat Pancreatic and Bone Cells (EKSTRAK JINTAN HITAM (Nigela sativa) MENGINDUKSI PROLIFERASI DAN DIFERENSIASI SEL PANKREAS DAN SEL TULANG TIKUS SECARA IN VITRO) Wahono Esthi Prasetyaningtyas; Deny Putra Romadhon; Fitri Susana; Ita Djuwita; Kusdiantoro Mohamad
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Black seed (Nigella sativa), a medicinal plant, widely used for treating various diseases, includingdiabetes mellitus and osteoporosis. This study examined the proliferation and differentiation of pancreaticand bone cells of rat cultured in vitro in medium supplemented with N. sativa extracts (NS). Pancreaticand bone cells were isolated from five days old rat and cultured in Dulbecco modified eagle mediumsupplemented without NS (0%, as control), and with NS (0.05% and 0.5%, as treatment groups) in 5% CO2incubator at 37oC for seven days and observed for cell population doubling time (PDT); proportion anddiameter of Langerhans islets, osteoblast, and osteocyte; and proportion of Langerhans islets containingb cell expressing insulin secretion. The pancreatic b cells were observed using dithizone staining, while thebone cells using alizarin red staining. The result showed that supplementation of NS significantly (p<0.05)decreased the PDT of pancreatic and bone cells, increased the proportion and diameter of Langerhansislets, increased the proportion of expression the b cell producing insulin, and increased the diameter ofosteoblast. In conclusion, the supplementation of NS in culture medium improved the proliferation anddifferentiation of pancreatic and bone cells in vitro.

Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi Ita Djuwita; Roza Helmita; Adi Winarto; Wahyudin -
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.

CARBOHYDRATES OF CHANGES DURING THE FOLLICULAR DEVELOPMENT IN THE OVARY OF THE MOUSE DEER, TRAGULUS JAVANICUS Hamny -; Srihadi Agungpriyono; Ita Djuwita; Chairun Nisa; Wahono Esthi Prasetyaningtyas; Erdiansyah Rahmi
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The data available on the female reproductive organ of mouse deer (Tragulus javanicus) is still very limited. A study was therefore conducted to investigate the distribution and the concentration of carbohydrate residues during the development of ovary follicles. An ovary at luteal phase was used in this study. Thin sections of the ovary were prepared occording to the standard methods and they were then histochemically stained with flourecnece-labelled lectins such as peanut agglutinin (PNA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), Winged bean agglutinin (WGA) and Ulex europaeus agglutinin (UEA). The result showed that changes in the distribution and the concentration of carbohydrate occured during the development of the follicle. During the preantral stage, the cytoplasm of oosit contained carbohydrate with the residues of glucosa dan mannosa. Zona pelusida contained carbohydrates with residues of glucosa, mannosa, galactosa dan N-asetylgalactosamine, whereas extracellular matrix contained carbohydrate with the residues of glucosa dan mannosa. In the antral follicle, the cyitoplasm of oocytes contained carbohydarte with the residues of galactosa dan N-asetylgalactosamine, whereas its zona pelusida, extracellular matrix and follicular fluid contained carbohydarte with the residues of fucosa, N-asetylglucosamin and cyalic acid. Diffrences in the types and the distribution pattern of carbohydrates were observed in this study, both in preantral and antral follicles.

CHALLENGING FOR SEAGRASS MANAGEMENT IN INDONESIA N adiarti; Etty Riani; Ita Djuwita; Sugeng Budiharsono; Ari Purbayanto; Harald Asmus
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Seagrasses, one of the important ecosystems in Indonesian coastal waters, have declined mostly due to a variety of multi-sector (i.e. ecology, socio-economy, technology and institution) anthropogenic disturbances. The decline and loss of seagrass meadows will have an effect not only on biodiversity and fisheries productivity within the ecosystems but also on the adjacent ecosystems (coral reef and mangrove forest), and even the effect will spread out far to the outside of the areas where seagrass grow. Seagrass ecosystems management in Indonesia is urgently required as part of fisheries management. However, this concept has not been understood by most of Indonesian people, including some government officials. Consequently, the seagrass ecosystems are still marginalized in the coastal resource management practices in Indonesia. In order to sustain fisheries productivity, knowledge of impact scales of each seagrass-related multi-sector human activities are very important as one of basic requirements in designing an effective seagrass management.

RESPONS SEL EPITEL USUS (CMT-93) TERHADAP NUTRASETIKAL GALOHGOR Katrin Roosita; Rimbawan R; Ita Djuwita; M. Rizal Damanik; Clara M. Kusharto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan membandingkan pengaruh nutrasetikal galohgor dalam bentuk serbuk (GS) dan ekstrak (GE) terhadap proliferasi, morfologi, dan ekspresi gen Aldh1a2 pada sel epitel usus (CMT-93). Galohgor serbuk (GS) dibuat dengan menghancurkan semua bahan dandikeringkan menggunakan drum-dryer sedangkan GE dibuat dengan mengeringkan semua bahan dengan oven, digiling, dan dimaserasi dengan etanol selama 3x24 jam. Perlakuan didasarkan pada konsentrasi akhir β-karoten yang berasal dari GS dan GE masing-masing sebesar 0,5; 1,5; dan 5,0 µM dalam larutan medium Dulbecco's Modified Eagle's medium (DMEM) yang dilengkapi serum (10%). Analisis proliferasimenggunakan asai 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dan ekspresi gen dianalisis dengan reverse transcriptasepolymerase chain reaction (RT-PCR). Hasil penelitian meunjukkan bahwa GE pada konsentrasi tinggi (5,0 μM) secara signifikan (P0,05) dapatmenekan proliferasi dan memengaruhi morfologi sel CMT-93. Beta-karoten dalam GE dan GS memengaruhi ekspresi gen Aldh1a2 pada sel epitel usus CMT-93

RESPONS SEL EPITEL USUS (CMT-93) TERHADAP NUTRASETIKAL GALOHGOR Katrin Roosita; Rimbawan R; Ita Djuwita; M. Rizal Damanik; Clara M. Kusharto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2825

Penelitian ini bertujuan membandingkan pengaruh nutrasetikal galohgor dalam bentuk serbuk (GS) dan ekstrak (GE) terhadap proliferasi, morfologi, dan ekspresi gen Aldh1a2 pada sel epitel usus (CMT-93). Galohgor serbuk (GS) dibuat dengan menghancurkan semua bahan dandikeringkan menggunakan drum-dryer sedangkan GE dibuat dengan mengeringkan semua bahan dengan oven, digiling, dan dimaserasi dengan etanol selama 3x24 jam. Perlakuan didasarkan pada konsentrasi akhir β-karoten yang berasal dari GS dan GE masing-masing sebesar 0,5; 1,5; dan 5,0 µM dalam larutan medium Dulbecco's Modified Eagle's medium (DMEM) yang dilengkapi serum (10%). Analisis proliferasimenggunakan asai 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dan ekspresi gen dianalisis dengan reverse transcriptasepolymerase chain reaction (RT-PCR). Hasil penelitian meunjukkan bahwa GE pada konsentrasi tinggi (5,0 μM) secara signifikan (P0,05) dapatmenekan proliferasi dan memengaruhi morfologi sel CMT-93. Beta-karoten dalam GE dan GS memengaruhi ekspresi gen Aldh1a2 pada sel epitel usus CMT-93

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