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All Journal Jurnal Penelitian Saintek Agrotrop : Journal on Agriculture Science Indonesian Journal of Biotechnology Jurnal Pendidikan Biologi Indonesia Bioeksperimen: Jurnal Penelitian Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Caraka Tani: Journal of Sustainable Agriculture Jurnal Bioedukatika Biosfer: Jurnal Pendidikan Biologi Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Jurnal Pertanian Agros AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Agroista: Jurnal Agroteknologi Kingdom (The Journal of Biological Studies) IJOBI
Ixora Sartika Mercuriani
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Neuroscience Physics Public Health

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Journal : IJOBI

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MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Inducing An Axillary Bud of Dendrobium Red Emperor ‘Prince’ With An Addition of Bap in Vitro Septasari, Mistri; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.216

Abstract

The purpose of this study is to determine the effect of the addition of BAP and the position of nodus on the formation of axillary shoots and to know the optimum concentration of BAP for the induction of Dendrobium Red Emperor 'Prince' axial shoots. The research design used is a Complete Factorial Randomized Design consisting of one treatment, namely: concentration of growing regulatory substances (ZPT) BAP (0ppm, 1 ppm, 2 ppm, and 3 pmm), each treatment repeated 5 times. The explant of the nodus were taken from plants produced by previous in vitro culture having 5 nodi. The basic medium used is New Phalaenopsis (NP) + Coconut Water + 1 ppm 2.4-D). The growth of axillary bud growth is measured based on the time of the bud emergence, growth of crown, and that of root. Data obtained were then analyzed using ANOVA. If there was a significant difference, the analysis was continued with a test of DMRT with a significant level of 5%. The results showed the addition of concentrations of 1 ppm and 2 ppm BAP influenced the time of the bud emergence, growth of crown, and that of root. The optimum concentration of BAP to induce the orchid axillary shoots is 2 ppm.
Co-Authors Afrizal Haris Agus Slamet Akram Akram Anisa Maulidiya* Annisa Ul Furqhoni Aries Bagus Sasongko Astuti Ayu Purnamasari Aziz Purwantoro Bekti Sulistya Utami Bernadetta Octavia Budiastuti, Ririn Candra, Puspa Mega Desi Indriani Dwi Kurniasari Eli Rohaeti ENDANG SEMIARTI Endang Semiarti Endik Deni Nugroho Endik Deni Nugroho Evy Yulianti Heru Nurcahyo HESTIN YUSWANTI Huang, Tzou-Chi Isana Supiah Yosephine Louise Khoirunnisa Khoirunnisa Kun Sri Budiasih Lili Sugiarto Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Madhani, Anisa Tiara Miftakhul Khasanah Nur Fitriyana Nurfina Aznam Paramita Cahyaningrum Kuswandi Puspa Mega Candra Putra, Rinaldi Rizal Ratna Dewi Ratnawati Ratnawati Ratnawati Ratnawati Ratnawati Retno Arianingrum Rifqi, Daffa Muhammad Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Seonghoe Jang Septasari, Mistri Setiawan, Andri Dias Sidabutar, Nurhalis Sofiatul Milah Sukarti Moeljopawiro Sukarti Moeljopawiro Suyitno Aloysius Vlorensius Vlorensius Wiharyanti, Risma Wiharyanti, Risma Wika Rinawati Yuyun Fitriani