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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Sewaka Bhakti Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Dharma Jnana

Ni Made Puspawati

Universitas Udayana

Author-ID : 2920749

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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KANDUNGAN KIMIA MINYAK ATSIRI DARI KULIT BUAH JERUK BALI (Citrus maxima) SERTA UJI AKTIVITAS ANTIBAKTERI TERHADAP Staphylococcus aureus DAN Escherichia coli Komang Ardipa Saputra; Ni Made Puspawati; I Wayan Suirta
Jurnal Kimia (Journal of Chemistry) Vol. 11. No. 1 Januari 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Penelitian ini bertujuan untuk menentukan aktivitas antibakteri minyak atsiri kulit buah jeruk Bali (Citrus maxima) dan mengidentifikasi komposisi senyawanya. Minyak atsiri kulit buah jeruk Bali diekstraksi menggunakan metode destilasi uap dan komposisi senyawanya diidentifikasi menggunakan KG-MS ( Kromatografi Gas –Spektrometer Massa). Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi agar terhadap bakteri Escherichia coli (E.coli ) dan Staphylococcus aureus (S.aureus). Minyak atsiri yang diperoleh dari ekstraksi kulit buah jeruk Bali berwarna bening, memiliki bau khas seperti jeruk dengan rendemen 0,14 %. Hasil uji aktivitas menunjukkan minyak atsiri kulit buah Jeruk Bali dapat menghambat pertumbuhan bakteri E.coli dan S.aureus. Pada konsentrasi 50, 75, dan 100 ppm minyak atsiri tersebut memberikan daya hambat yang kuat terhadap E.coli dengan diameter hambat berturut-turut, 11, 14, dan 17mm. Namun aktivitas antibakterinya terhadap S.aureus pada 50 ppm tergolong sedang dengan daya hambat 9 mm, dan aktivitas yang kuat ditunjukkan pada konsentrasi 75 dan 100 ppm dengan daya hambat berturut-turut 11 mm dan 14 mm. Hasil analisis spektra KG-MS menunjukkan minyak atsiri kulit buah Jeruk Bali mengandung lima senyawa yang teridentifikasi sebagai senyawa ?-pinen, mirsen, limonen, germakren dan ?-asaron

AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus N. M. Puspawati; N. L. P. F. Widiari; I M. Sukadana
Jurnal Kimia (Journal of Chemistry) Vol.14 No.1 Januari 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly. Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.

SINTESIS NANOKOMPOSIT LEMPUNG BENTONIT-ASAM SALISILAT DENGAN METODE SOL-GEL DAN KARAKTERISASINYA DENGAN XRD DAN IR-FTIR Ida Ayu Gede Widihati; Ni Putu Diantariani; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 11. No.2 Juli 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Dalam penelitian ini dilakukan sintesis nanokomposit lempung bentonit-asam salisilat dengan metode sol gel melalui mekanisme interkalasi. Pada proses interkalasi ini, asam salisilat harus dibuat dalam bentuk senyawa kompleks dengan ion Fe(III) agar asam salisilat dapat masuk ke ruang antar lapis lempung. Pada proses interkalasi tahap pertama, konsentrasi interkalat divariasikan dengan tujuan mendapatkan nanokomposit yang memliki karakter kimia fisik yang terbaik. Sifat kimia fisik nanokomposit lempung bentonit-asam salisilat dikarakterisasi dengan menggunakan metode difraksi sinar X, FTIR. Pada tahap kedua dilakukan proses interkalasi dengan waktu reaksi yang bervariasi. Dari data XRD didapatkan bahwa variasi konsentrasi interkalat tidak menyebabkan perubahan kristalinitas yang signifikan. Ketiga komposit yang terbentuk menunjukkan puncak difraktogram yang sama dengan bentonit awal. Interkalasi asam salisilat ke dalam ruang antar lapis lempung bentonit menyebabkan peningkatan jarak antar lapis (d basal spacing) menjadi 16-17Å. Dari spektra IR dapat dilihat beberapa pita absorpsi yang karakteristik untuk lempung dan modifikasinya. Pita serapan pada bilangan gelombang 3620 cm-1 menunjukkan vibrasi stretching dari –OH untuk Al-OH pada montmorillonit. Bilangan gelombang 1640 cm-1 mengindikasikan adanya –OH bending dari air yang terabsorpsi. Pada ketiga spektra di atas terjadi pergeseran bilangan gelombang ke, (a). 1631,78 cm-1 (b). 1635,64 cm-1 dan (c). 1633,7 cm-1. Pita serapan pada bilangan gelombang 1115 cm-1 mengindikasikan adanya Si-O stretching untuk lapisan-lapisan silikat. Pada nanokomposit yang dihasilkan terjadi pergeseran bilangan gelombang tersebut ke 1109,7 cm-1(a), 1111cm-1 (b dan c). Pita serapan pada bilangan gelombang 1633,71 cm-1, 1631,78 cm-1dan 1635 cm-1 pada ketiga spectra di atas menunjukkan adanya vibrasi dari gugus C=O yang berasal dari asam salisilat. Adanya pita serapan pada bilangan gelombang 920 cm-1 mengindikasikan vibrasi bending dari Al-Al-OH. Karakterisasi dengan FTIR menunjukkan bahwa pemanasan dari 120°C hingga 250°C tidak mengakibatkan perubahan struktur yang ditunjukkan dengan tidak adanya pergeseran pita vibrasi yang dimilki oleh masing-masing gugus fungsi dari nanokomposit lempung bentonit asam salisilat

ISOLASI, IDENTIFIKASI, SERTA UJI AKTIVITAS ANTIBAKTERI PADA MINYAK ATSIRI SEREH WANGI (Cymbopogon winterianus Jowitt) Ni Made Puspawati; I Wayan Suirta; Saeful Bahri
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 2 Juli 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Sereh wangi (Cymbopogon winterianus Jowitt) merupakan salah satu tanaman yang dimanfaatkan sebagai obat tradisional. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dan mengidentifikasi komposisi senyawa kimia minyak atsiri yang diekstraksi dari daun dan batang sereh wangi. Ekstraksi minyak atsiri dilakukan menggunakan metode destilasi uap dan komposisi kimianya diidentifikasi dengan KG-SM (Kromatografi Gas-Spekrometer Massa). Minyak atsiri yang diekstrak dari daun dan batang sereh wangi berwarna kuning muda dengan rendemen berturut-turut 0,31% dan 0,10%. Hasil uji aktivitas antibakteri terhadap bakteri Eschericia coli dan Staphylococcus aureus menunjukkan bahwa aktivitas antibakteri minyak atsiri daun dan batang sereh wangi bergantung pada konsentrasinya. Pada konsentrasi minimum 25 ppm, minyak atsiri daun aktif dengan diameter hambat sebesar 4,0 mm untuk E.coli dan 3,0 mm untuk S.aureus. Aktivitas penghambatan yang kuat terhadap bakteri E.coli didapatkan pada konsentrasi 100 ppm (10,25 mm pada daun dan 10,62 mm pada batang) dan terhadap S.aureus pada konsentrasi 100 ppm (11,25 mm pada batang). Hasil identifikasi dengan KG-SM, minyak atsiri pada daun dan batang memberikan 12 puncak senyawa dengan waktu retensi yang sama dengan luas area yang berbeda pada kromatogramnya. Hal ini menunjukkan komposisi kimia yang sama pada minyak atsiri daun dan batang sereh wangi. Berdasarkan hasil identifikasi dapat disimpulkan bahwa komponen utama dari minyak atsiri daun dan batang sereh wangi adalah sitronellal, cis-sitral, geraniol dan geranil asetat.

itu PRODUKSI HIDROLISAT PROTEIN ANTIOKSIDAN MELALUI HIDROLISIS ENZIMATIK PROTEIN KULIT AYAM BROILER DENGAN ENZIM PAPAIN N. M. Puspawati; P. P. Dewi; N. W. Bogoriani; N. K. Ariati
Jurnal Kimia (Journal of Chemistry) Vol. 14, No. 2 Juli 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2020.v14.i02.p16

Penelitian ini bertujuan untuk menentukan aktivitas antioksidan hidrolisat protein yang dihasilkan dari proses hidrolisis enzimatik protein kulit ayam dengan menggunakan variasi konsentrasi enzim papain. Proses hidrolisis dilakukan dengan variasi konsentrasi enzim 1, 3,dan 5% (b/b protein), pada pH 7, suhu 50oC dan waktu hidrolisis 24 jam. Parameter yang diukur meliputi derajat hidrolisis, persen peredaman radikal DPPH (diphenylpicryl hidrazyl), dan komposisi asam amino hidrolisat protein yang paling aktif antioksidan. Hasil penelitian menunjukkan penggunaan konsentrasi enzim yang berbeda menghasikan hidrolisat protein dengan derajat hidrolisis dan persen peredaman DPPH yang berbeda. Penggunaan konsentrasi enzim papain 1, 3, dan 5% menghasilkan hidrolisat protein dengan derajat hidrolisis berturut-turut sebesar 9,3±1,8, 18,09±5,6, dan 23,15±6.33%, dan persentase hambatan radikal DPPH pada konsentrasi uji 100 ppm berturut-turut sebesar 16,75±0,07, 58,35±0,00, dan 52,99±0,07%. Hidrolisat protein yang diperoleh menggunakan enzim papain 3% (b/b protein) menunjukkan persen peredaman tertinggi dengan nilai IC50 92,98 ppm, memiliki komposisi asam amino tertiggi asam glutamat dan terendah valine. Kata kunci: antioksidan, enzim papain, hidrolisat protein, kulit ayam This study aimed to determine the antioxidant activity of protein hydrolyzate produced from the enzymatic hydrolysis of chicken skin protein by using variations of the enzyme papain concentration. The hydrolysis process was carried out with variations in the concentration of the enzymes 1, 3, and 5% (w/w protein base), at pH 7, temperature 50oC, and hydrolysis time 24 hours. The parameters measured including degree of hydrolysis, percentage of inhibition of DPPH radical (diphenylpicryl hydrazyl) and amino acid composition of the protein hydrolyzate with the highest antioxidant activity. The results showed that the use of different enzyme concentrations resulted in protein hydrolyzate with different degrees of hydrolysis and percentage of inhibition of DPPH. The use of papain enzymes 1, 3, and 5% produced protein hydrolyzate with the degree of hydrolysis of 9.3 ± 1.8, 18.09 ± 5.6, and 23.15 ± 6.33%, and the percentage of inhibition of DPPH radical at a concentration of 100 ppm 16.75 ± 0.07, 58.35 ± 0.00, and 52.99 ± 0.07% respectively. Protein hydrolyzate obtained using the enzyme papain 3% (w/w protein) exhibited the highest percentage of inhibition of DPPH with an IC50 value of 92.98 ppm, having the highest amino acid composition of glutamic acid and the lowest valine. Keywords: antioxidant, enzyme papain, protein hydrolyzate, chicken skin

ISOLASI DAN IDENTIFIKASI SENYAWA AKTIF LARVASIDA DARI BIJI MIMBA (Azadirachta indika A. Juss) TERHADAP LARVA NYAMUK DEMAM BERDARAH (Aedes aegypti) I W. Suirta; N. M Puspawati; N. K. Gumiati
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 2 Juli 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.573 KB)

Isolation and identification of larvicidal active compounds towards Aedes aegypti from Mimba seed havebeen conducted. One kilogram dry powder of Mimba seed was extracted with ethanol at room temperature.Evaporation of ethanol gave 30 g of crude ethanol extract which showed activity against Aedes aegypti (LC50282.29). This extract was dissolved into methanol-water (7:3) and was then partitioned with n-hexane, chloroformand ethyl acetate respectively. The three extracts obtained i.e. n-hexane, chloroform and ethyl acetate were showedtheir activity against Aedes aegypti in which the-n-hexane extract was the most active with LC50 of 143.97.Therefore the n-hexane extract was further purified using silica gel column chromatography with chloroform:nhexane(9:1) as eluent. Three fractions was obtained i.e. F1, F2 and F3 and they were all active against Aedes aegyptiwith LC50 78.45, LC50 113.54 and LC50 58.70 respectively. It can be seen that F3 was the most active fractions butfrom TLC result, F1 showed the relatively pure compounds since it only gave one spot. Therefore F1 was furtheridentified using pyhtochemical testing, Uv-Vis, infrared and GC-MS. It was found that the larvicidal activecompounds F1 was belong to carboxylic acids groups,with l max 290.1 having functional groups such as methyl,methylene and carbonil. Identification using GC-MS indicated that the larvicidal active compounds F1 was assumedto be a combination of 7 compounds derived from carboxylic acids including hexa-decanoic acid, etil-hexadecanoate,oleic acid, etyl-oleate ester, octadecanoic acid, etyl-octadecanoate and dioctyl-hexadioate.

KOMPOSISI ASAM AMINO DAN POLA PITA PROTEIN GELATIN HALAL DARI KULIT AYAM BROILER Ni Made Puspawati; Ida Ayu Gede Widihati; I Nyoman Widana
Jurnal Kimia (Journal of Chemistry) Vol. 11. No. 1 Januari 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Penelitian ini bertujuan untuk mempelajari pengaruh perbedaan jenis asam yang digunakan pada proses perendaman terhadap komposisi asam amino, pola pita protein dan kekuatan gel produk gelatin yang diekstrak dari kulit ayam Broiler. Pada penelitian ini asam yang digunakan pada proses perendaman yaitu asam asetat (AA), sitrat (AS), dan laktat (AL). Komposisi asam amino produk gelatin dianalisis dengan HPLC, pola pita protein dianalisis menggunakan metode elektroforesis, dan kekuatan gel diukur dengan CT3 Texture Analyzer. Hasil analisis menunjukkan, ketiga produk gelatin yaitu GAA, GAL,dan GAS mempunyai komposisi asam amino yang tidak jauh berbeda dengan persentase tertinggi glisin, diikuti prolin, glutamat, arginin, dan alanin sedangkan terendah metionin, sistin dan tirosin, serta tidak terdeteksi adanya histidin. Persentase glisin (18,31%) dan prolin (9,18%) untuk GAA tidak berbeda secara signifikan dengan GAS (17,88% dan 8,85%) dan GAL (17,77% dan 7,46%). Elektroforegram SDS-PAGE untuk GAA dan GAS hampir sama yaitu memberikan pita protein dengan berat molekul tinggi pada 85kDA yang diikuti dengan pita protein dengan berat molekul lebih rendah pada 66, 45, 31, dan 25kDA namun pita protein pada GAA lebih tebal dari GAS. GAL tidak menunjukkan pita protein dengan berat molekul yang tinggi, hanya agregates protein dengan berat molekul rendah pada 21 kDA. Hasil pengukuran kekuatan gel untuk GAA 216,63 g bloom, GAS 109,01g bloom, dan GAL 32,73 g bloom. Dari penelitian ini, dapat disimpulkan bahwa jenis asam yang digunakan pada proses perendaman tidak berpengaruh terhadap persentase kandungan asam amino tetapi berpengaruh terhadap pola pita proteinnya (berat molekul) dan kekuatan gel dari produk gelatin yang dihasilkan.

IDENTIFIKASI SENYAWA AKTIF ANTIFEEDANT DARI EKSTRAK DAUN PANGI (Pangium Sp) DAN UJI AKTIVITASNYA TERHADAP ULAT KUBIS (Plutella Xylostella) Ida Bagus Putra Mahardika; Ni Made Puspawati; Ida Ayu Gede Widihati
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.236 KB)

Leaves of pangi (Pangium edule) are known to have many benefits, including antifeedant activity. 500 g of pangi leaf powder was extracted by maceration method using n-hexane extract yieled 4.51 g of dark green extract. This extracts showed high antifeedant activity (47.23%) against Plutella Xylostella at a concentration of 0.1% (w / v). Separation of 2 g of n-hexane extract by chromatography column using n-hexane: ethyl acetate: chloroform (6:2,5:1,5) as mobile phase and silica gel 60 as stationary phase produced 6 groups of fractions. Fractions 3 reveiled the highest antifeedant activity that is 52.15%, 69.88%, and 91.66% respectively at a concentration of 10 ppm, 100 ppm, and 1000 ppm. This active fraction which was considered relatively pure following purity test on TLC was then identified using GC-MS spectrometre. GC-MS spectra of antifeedant active compound contained at least 11 compounds with 8 compounds successfully identified as ? – pinene, benzene trimethyl, acetic acid triflouro-tetradecyl ester, nonadecene, 13-Hexyloxacyclotridec-10-en-2-one, phytol, 3-eicosene, , benzenedicarboxylic acid diisooctyl este.

KAJIAN PENGARUH VARIASI KONSENTRASI ASAM SITRAT TERHADAP KEKUATAN GEL PRODUK GELATIN KULIT AYAM BROILER DIKAITKAN DENGAN POLA PROTEINNYA Tutut Hardikawati; Ni Made Puspawati; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Gelatin is a biopolymer that can be generated from partially hydrolysis of collagen tissue. Extraction of gelatin consists of pretreatment and thermal extraction steps. Pretreatment process used sodium hydroxide to remove non collagen protein in matrix sample, sulfuric acid to demineralize, and citric acid to hydrolyse. The aim of this research was to study the effect of variation in concentrations of citric acid used in hydrolysis process on the gel strength and protein profile of gelatin products extracted from broiler chicken skin. The variation of the concentration citric acid used was 0.7 % (GA); 1.5% (GB); and 3.0% b/v (GC) respectively. The gel strength was measured using CT3 Texture Analyzer and protein profile of gelatin product was analyzed by SDS-PAGE method. The result showed that variation in concentration of citric acid used in the pretreatment process affected the gel strength and protein profile of gelatin product. Increasing the concentration of citric acid used in pretreatment process decreased the gel strength and molecular weight of gelatin product. Gel strength of each gelatin product was 265.81 g bloom for GA ; 196.05 g bloom for GB (1.5%), and 35.32 g bloom for GC (3.0 %) respectively. The electropherogram of both GA (0.7%) and GB (1.5%) revealed similar pattern of protein bands but the thickness of each bands was different. On the other hands, GC (3.0%) did not show any protein bands on the eletropherogram. The best gelatin product obtained in this experiment was found by using 0.7 % b/v citric acid (GA) in the pretreatment process. The gelatin product (GA) had characteristics as follows: yield 15.73%; moisture 7.30%; ash 0.51%; protein content 97.95%; fat content 0.62%; gel strength 265. 81 g bloom and thicker protein bands than others.

IDENTIFIKASI GOLONGAN SENYAWA AKTIF ANTIMAKAN DARI DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP LARVA Epilachna sparsa L M. G. Agus Mandana; Ni M. Puspawati; Sri Rahayu Santi
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Protium is the main genusl in the family of Burseraceae which has been used traditionally as medicine, cosmetic as well as insecticide. This research aimed to isolate and identify antifeedant active compounds from leaf of Protium javanicum Burm, F which is locally known as tenggulun. Epilachna sparsa (E.sparsa) larvae were used as bioindicator for antifeedant activity assay. The assay was performed both on crude extract, fractions, and isolate. Dried leaf powder of tenggulun (1000 g) was extracted with methanol to yield 20.89 g dark green extract. This extract showed 71.61% antifeedant activity at 0.1% (b/v). This active extract was then fractionated into n-hexane, chloroform and water fractions. The chloroform fraction was the most active which give 89.62% activity at 0.1% (b/v) compared to n-hexane (36.83%), and water fractions (1.96 %). Purification of chloroform fraction was done using silica gel column chromatography with n-hexane : chloroform (3:4) as eluent and three groups of fractions (isolates) were obtained. The most active isolate which showed 70.53% antifeedant activity at 10 ppm was found relatively pure, therefore it was then identified. Based on phytochemical reaction, analysis of infrared spectra and ultraviolet-visible spectra, the antifeedant active isolate was identified as triterpenoid class of compounds which have functional group such as OH, CH3, CH2, C=O, C=C and ?max at 245 and 416 nm.

Co-Authors A A I Rahma Prabawanti A. A. Bawa Putra A. A. Tia Santika Dewi Anak Agung Istri Agung Mayun Laksmiwati Arisma Damayanti Ashri Rizki Hidayati Desak Putu Eka Nilakusmawati Dewa Ayu Indra Dewi Diah Prihatiningsih G. A. G. Indukirana Gusti Ayu Primandari Utami Haqqika Pasha Helen Helda Prastika I G. P. Sukmajaya A. P. T. I Gede Tangkas Mei Yasa I Gusti Putu Agus Ferry S.P. I K. D. Yasa I M, Sukadana I M. Sukadana I Made Dira Swantara I Made Oka Adi Parwata I Made Sudarsana I Nengah Simpen I Nyoman Suarsana I Nyoman Sumerta Miwada I Nyoman Widana I Wayan Karta I Wayan Pramana Eka Putra I Wayan Suirta I. A Raka Astiti Asih I. A. Gede Widihati I. A. R. Astiti Asih IDA AYU ASTARINI Ida Ayu Gede Widihati Ida Ayu Putu Sri Adnyasari Ida Bagus Putra Mahardika Ida Bagus Putra Manuaba Indriani Wisnu Susanto Panjaitan Irdhawati Irdhawati James Sibarani K. Sari K. Swandiyasa Ketut Ratnayani Ketut Ratnayani Komang Ardipa Saputra M. G. Agus Mandana Mahardika Aprilia Iflahah Manuntun Manurung N. K. Gumiati N. L. P. F. Widiari N. W. Bogoriani Ni G. A. M. Dwi Adhi Suastuti Ni Ketut Puspa Sari Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Mega Wahyuni Ni Luh Putu Putri Setianingsih Ni Luh Putu Suciptawati Ni Luh Rustini Ni Made Suaniti Ni Putu Adriani Astiti Ni Putu Diantariani Ni Putu Rahayu Kusuma Pratiwi Ni Putu Rusma Eva Arista Ni Putu Widayanti Ni Wayan Deswiniyanti Ni Wayan Oktarini A.C.Dewi Oka Ratnayani Oscar Yonathan Hambiyono P. P. Dewi Paramartha, I. D. G. Y. Putu Puspita Sari Putu Sariningsih Putu Suarya Riana Dyah Suryaningrum Riski Fatur Rachman Saeful Bahri Sakinatul Fatimah Simalango, N. T. H. Sri Rahayu Santi Sri Wahjuni Sri Wahjuni Tutut Hardikawati Wijaya, P. S. Wiwik Susana Rita WIWIK SUSANAH RITA Yenni Ciawi

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