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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Sewaka Bhakti Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Dharma Jnana

Ni Made Puspawati

Universitas Udayana

Author-ID : 2920749

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK DAUN TREMBESI (Samanea saman (Jacq.) Merr) SEBAGAI PENGENDALI JAMUR Fusarium sp. PADA TANAMAN BUAH NAGA Putu Sariningsih; Wiwik Susanah Rita; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study aimed to examine antifungal activity of Trembesi (Samanea saman (Jacq.) Merr) leaves extract against Fusarium sp on dragon fruit and to identify types of flavonoid compounds present in the extracts. Isolation of the flavonoids was started by maceration followed by fractionation into n-hexane, chloroform, and ethyl acetate respectively. Separation was carried out by preparative layer chromatography while identification was done using Ultraviolet-Visible and Infrared spectrophotometer. Antifungal activity test showed that ethylacetate extract has mild activity in inhibiting the growth of Fusarium sp. (inhibition zone 6.75 mm). The antifungal activity testing of three isolates positive flavanoid (B4, B5, B6) showed at the concentration of 10 % they have not given activity yet. The infrared spectra of isolates (B4, B5, B6) were very similar, therefore they have the same functional groups (OH, C-OH, aromatic CH, aliphatic CH, C=O, C-O-C ether, and aromatic C=C). The UV-Vis spectra showed isolates B4 gave absorption at a wavelength of 336.00 nm (band I) and 268.40 nm (band II), isolates B5 at 269.20 nm (bands II), and 325.40 nm (band I), and isolates B6 at 475.40 nm (bandI) and 282.40 nm (band II). Further UV-Vis identification using shift reagents suggested that isolates B4 was tentatively identified as 3,7,8,4 ', 5' pentahydroxy flavonols, isolates B5 as 3,5,4 'trihydroxy flavones, and isolate B6 as 3,5,7,8,3', 4 'hexahidroxy anthocyanine.

ANALISIS KANDUNGAN MINYAK ATSIRI DAUN TENGGULUN (Protium javanicum Burm.F.) DENGAN METODE KROMATOGRAFI GAS-SPEKTROSKOPI MASSA I G. P. Sukmajaya A. P. T.; N. M. Puspawati; A. A. Bawa Putra
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.44 KB)

Leaves of Tenggulun are commonly used in traditional medicine for inflammatory therapy. This research aims to analyze the chemical composition of the volatile oil extracted from Tenggulun leaf by gass chromatography (GC) coupled to mass spectrometer (MS). The volatile oil was obtained by steam distillation method. GC-MS spectra demonstrate that the volatile oil of Tenggulun leaf is constituted mainly of monoterpenes such as ?-ocimene (49.87%) and ?-pinene (0.36%) and sesquiterpenes such as ?-caryophyllene (24.95%), germacrene (4.01%), ?-humulene (2.98%), ?-elemene (2.38%), caryophyllene oxide (0.81%), ?-amorphene (0.46%), and spathulenol (2.64%).

UJI TOKSISITAS MINYAK ATSIRI DAUN TENGGULUN (Protium javanicum Burm. F.) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) Ni Luh Putu Putri Setianingsih; I Wayan Suirta; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 2 Juli 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Toxicity of essential oils extracted from young and old leaves of Tenggulun (Protium javanicum, Burm) towards Artemia salina leach larvae has been evaluated using Brine Shrimp Lethality Test and their chemical compositions have been analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The essential oils were obtained by extracting fresh young and old leaves of Tenggulun using steam distillation method. Steam distillation of young leaves produced oils with 0.06% yield while old leaves yielded 0.07 % oils. The toxicity test results found thatthe essential oils obtained from old leaves were more toxic with LC50 of 15.85 compared to the essential oils of young leaves whose LC50 was 25.12 ppm. GC-MS data revealed that the essential oils of young leaves contained 11 compounds which were : ?-pinene (0.75 %), ?-pinene (0.54 %), delta-3-carene (0.38 %), octatriene (1.35%), trans-?-ocimene (77.63 %), ?-elemene (1.23 %), trans-caryophyllene (12.62 %), ?-humulene (1.18 %), germacrene (3.25 %), ethanone (0.65 %) and isospathulenol (0.42 %) while the essential oils of old leaves composed of 21 compounds whichwere : ?-pinene (0.71 %), ?-pinene (0.44 %), ?-myrcene (0.15 %), cis-ocimene (2.06 %), trans-?-ocimene (52.79 %), trans-caryophyllene (30.02 %), ?-humulene (2.80 %), germacrene (0.28 %), germacrene (4.64 %), ?-farnesene (0.85 %), ?-elemene (0.16 %), tridecatrienenitrile (0.19 %), ethanone (0.49 %), caryophyllene oxide (0.83 %), nerolidol (0.12 %), spathulenol (1.25 %), isospathulenol (1.18 %), kauran (0.30 %), isospathulenol (0.16 %), spathulenol (0.34 %) and ?-farnesene (0.23 %).

AKTIVITAS ANTIPIRETIK EKSTRAK ETANOL KULIT BUAH RAMBUTAN (Nephelium lappaceum L) SECARA IN VIVO DAN KANDUNGAN FENOLIK TOTALNYA I Made Dira Swantara; Riski Fatur Rachman; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 11. No.2 Juli 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Penelitian ini bertujuan untuk mengetahui efek antipiretik ekstrak etanol kulit buah rambutan dan menentukan kandungan fenolik totalnya. Metode untuk uji aktivitas antipiretik dilakukan secara invivo menggunakan ragi tape untuk menginduksi demam pada tikus jantan galur Wistar dan menggunakan metode Folin-Coicalteu untuk mengukur total fenolnya. Hasil skrining fitokimia menunjukkan adanya senyawa golongan fenolik, tanin, flavonoid, saponin, dan terpenoid, dengan kandungan senyawa fenoliknya yang lebih dominan. Hasil penelitian menunjukkan pemberian ekstrak etanol kulit buah rambutan pada dosis 5; 10 dan 20 mg/100 g BB bersifat antipiretik dengan terjadinya penurunan suhu rectal tikus dibandingkan dengan kontrol negatif dan penurunan suhu yang tajam terlihat pada menit ke-210 setelah pemberian ekstrak. Kandungan fenol total dalam ekstrak etanol kulit buah rambutan yang diperoleh sebesar 39,7861 g GAE/100 g atau 39,78%.

UJI TOKSISITAS EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) N. M. Puspawati; I K. D. Yasa; I. A. R. A. Asih
Jurnal Kimia (Journal of Chemistry) Vol. 15, No.2, Juli 2021
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2021.v15.i02.p15

Tumbuhan tenggulun (Protium javanicum Burm F.) secara tradisional telah digunakan untuk mengatasi berbagai macam penyakit seperti batuk, perut nyeri, diare, dan radang. Penelitian ini dilakukan dengan tujuan untuk menentukan toksisitas ekstrak n-heksana, etil asetat, n-butanol daun tenggulun terhadap larva Artemia salina L dan mengidentifikasi senyawa aktifnya. Toksisitas ekstrak ditentukan dengan metode Brine Shrimp Lethality Test (BSLT) dan identifikasi senyawa aktifnya dengan LCMS/MS (Liquid Chromatography tandem Mass Spectrometry/Mass Spectrometry). Maserasi 1000 g daun tenggulun dengan metanol menghasilkan 116,9936 g ekstrak kasar metanol. Partisi ekstrak kasar metanol memakai pelarut n-heksana, etil asetat, dan n-butanol masing-masing menghasilkan ekstrak kental n-heksana, etil asetat, dan n-butanol. Hasil uji toksisitas terhadap ektrak n-heksana, etil asetat, dan n-butanol diperoleh nilai LC50 sebesar 218,78; 134,90; dan 223,87 ppm. Ekstrak etil asetat menunjukkan toksisitas yang relatif lebih tinggi dibandingkan ekstrak lainnya. Pemisahan senyawa aktif pada ektrak etil asetat dilakukan dengan metode kromatografi vakum cair (KVC) dengan fase diam silika gel dan fase gerak (n-heksana 100% sampai metanol 100 %) menghasilkan 5 fraksi (FA, FB, FC, FD, dan FE). Toksisitas tertinggi pada konsentrasi uji 100 ppm ditunjukkan oleh FA dengan persentase kematian larva 83,33%. Hasil analisis spectra LC-MS/MS dari fraksi aktif ekstrak etil asetat daun tenggulun FA menunjukkan adanya kandungan senyawa aktif yang diduga sebagai benzodioxepin-7-yl-7H-furo[3,2-g]chromen-7-one dan 10-(1,3-benzodioxol-5-yl)-9H-[2]benzofuro[6,5-g][1,3]benzodioxol-7-one. Kata kunci: Tenggulun (Protium javaniccum Burm F), toksisitas, Brine Shrimp Lethality Test, LC-MS/MS

KARAKTERISTIK MUTU GELATIN DARI KULIT AYAM BROILER MELALUI PROSES PERENDAMAN KOMBINASI ASAM-BASA I Nengah Simpen; Ni Made Puspawati; A A I Rahma Prabawanti
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 2 Juli 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Kandungan kolagen yang cukup tinggi pada kulit ayam berpotensi untuk dikembangkan sebagai bahan baku alternatif gelatin halal. Penelitian ini bertujuan untuk mempelajari pengaruh variasi pelarut asam, yaitu asam asetat, asam laktat dan asam sitrat dengan konsentrasi masing-masing 1% setelah perendaman basa NaOH 0,15% terhadap rendemen, pH, viskositas, dan kekuatan gel produk gelatin yang diekstrak dari kulit ayam broiler. Proses perendaman yang menghasilkan gelatin terbaik dipilih berdasarkan acuan standar mutu, kemudian gelatin tersebut dikarakterisasi sifat fisikokimianya dan dibandingkan dengan gelatin komersial. Penelitian ini disusun menggunakan rancangan acak lengkap (RAL) enam perlakuan dengan tiga kali ulangan dan dianalisis dengan metode one-way ANOVA. Hasil penelitian menunjukkan perbedaan perlakuan pada proses perendaman tidak berpengaruh nyata (P>0,05) terhadap hasil rendemen, sedangkan terhadap pH, viskositas, dan kekuatan gel berpengaruh nyata (P<0,05). Gelatin terbaik dihasilkan dari proses perendaman dengan NaOH 0,15% dilanjutkan dengan asam asetat 1%. Analisis sifat fisikokimia gelatin terpilih menunjukkan hasil yang tidak berbeda dengan gelatin komersial, serta memenuhi standar mutu SNI dan British Standard dengan karakteristik pH 5,20; viskositas 4,20 cP; kekuatan gel 239,92 g bloom; kadar air 11%; kadar abu 1,08%; kadar protein 91,82%; dan kadar lemak 0,99%. Hasil analisis spektra FTIR menunjukkan gugus fungsi khas gelatin pada sampel, yaitu gugus O-H, N-H, C-O, C=O, C-N dan NCO.

IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA TANIN DARI EKSTRAK DAUN TREMBESI (Samanea saman (Jacq.) Merr) SEBAGAI ANTIBAKTERI Escherichia coli (E. coli) Putu Puspita Sari; Wiwik Susanah Rita; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation and identification of tannin compounds from trembesi leaves (Samanea saman (Jacq.) Merr) and its anti-bacterial activity test against Escherichia coli (E. coli) have been done in this research based on the utilization of trembesi leaves to treat diarrhea. Extraction was done by maceration and partition, separation by preparative TLC. The anti-bacterial activity was tested using wells that diffusion method, and the identification of the compounds was done with UV-vis spectrophotometer and FTIR. Maceration with ethanol produced 36.80 g crude ethanol extract. Phytochemical test showed that acetone and water fractions gave positive result for hydrolyzed tannin compounds but acetone fraction revealed more concentrated than water fraction. Anti-bacterial test result showed that the acetone fraction was active towards E.coli with medium activity. Separation of the eluent n-butanol: acetic acid: water (4:1:5) (BAA) gave six isolates but only two isolates (isolate 2 with Rf 0.61, and isolates 3 with Rf.0.65) gave positive results for tannin. These two isolates were relatively pure on TLC purity test and showed weaker anti-bacterial activity compared to the acetone fractions. Identification using UV-Vis spectrophotometer showed that isolates 2 and 3 gave two similar peaks with maximum absorbance at 346.50 nm and 347.00 nm respectively due to n?*and ??* electron transitions, which indicated the presence of C=O and C=C chromophores. Infrared spectra of isolates 2 and 3 revealed peaks that correspond to characteristic functional groups of tannin including –O-H, C-H aliphatic, C=O esther, C=C aromatic, C-O-H, and C-O-C ether.

OPTIMASI DEASETILASI KHITIN DARI KULIT UDANG DAN CANGKANG KEPITING LIMBAH RESTORAN SEAFOOD MENJADI KHITOSAN MELALUI VARIASI KONSENTRASI NaOH N. M. Puspawati; I N. Simpen
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 1 Januari 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.363 KB)

Chitin resulted from treatment of shrimp and crab shells wasted of seafood restaurant, can be transformedinto chitosan through deacetylation process using concentrated NaOH. This research aims to investigate the optimumcondition of the deacetylation process to achieve a high yield and deacetylation degree of chitosan by varying theconcentration of NaOH while temperature and length of reaction are made constant at 120oC for 4 hours. Theconcentration of NaOH used in this research was 50; 55; and 60%, respectively. All chitosan obtained were purifiedand characterized by FTIR. The deacetylation degree of chitosan was calculated based on FTIR spectra data.The result found, the optimum condition for deacetylation of chitin from shrimp shells at 120oC for 4hours, was achieved using NaOH 60%, giving a high yield of 54.90% and deacetylation degree of 88.04%.. Usingthe same condition as for deacetylation chitin from shrimp, chitosan obtained from crab shells gave a better yieldwhich was 62.76 %, and the deacetylation degree of 88.53%. Both chitosans obtained from shrimp and crab shellssolubled in a 2% acetic acid solution.

MINYAK ATSIRI DAUN TENGGULUN (Protium javanicum Burm.F.) SEBAGAI REPELAN NYAMUK DEMAM BERDARAH (Aedes aegypti) Gusti Ayu Primandari Utami; Sri Rahayu Santi; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Tenggulun leaves (Protium javanicum Burm.F.) have been used traditionally as a medicine and insecticide. Activity test of essential oils from Tenggulun leaves as repellent against Aedes aegypti was done at concentrations of 7.5%, 15%, 20%, and 40% in 96% ethanol to determine its effectiveness. The essential oils were obtained by steam distillation methods, while the repellent activity test was conducted using Aedes aegypti adult females. Isolation of 12 kg fresh tenggulun leaves using steam distillation yielded 0.07% yellowish oils (13.7 mL) with the density of 0.8774 g/mL and sour sting smell. Gas chromatogram of the essential oils indicated that there were 13 compounds and identification of each compounds based on their mass spectra suggested that the compounds were ?-pinene (16.85%), myrcene (1.53%), ?-phellandrene (45.34%), p-cymene (5.60%), limonene (15.70%), ?-ocimen (0.34%), bicyclogermacrene (1.61%), ?-elemene (2.27%), ?-caryophyllene (7.90%), ?-humulene (0.88%), germacrene (1.50%), spathulenol (0.23%), caryophyllene oxide (0.24%). The repellent test result analyzed by One Way ANOVA indicated that at each concentration of the essential oils given showed significant differences during 6 hours treatment. Furthermore Tukey HSD test result showed that the essential oils at concentration of 40% revealed repellent activity which was comparable to positive control at the start of the test until the fifth hour of testing. Four of the compounds which are ?-pinene, myrcene, limonene and ?-caryophyllene have been known to have activity as repellent against mosquitoes.

ANALISIS ASAM LEMAK RUMPUT LAUT Ulva reticulata Forsskal YANG DIPEROLEH DARI PANTAI SEGARA SANUR Ni Made Puspawati; Ni Gusti Ayu Made Dwi Suastuti; Dewa Ayu Indra Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 2 Juli 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.564 KB)

Lipid content and fatty acid composition of seaweed Ulva reticulata Forsskal obtained from Segara Sanur Beach have been analyzed. Lipid was extracted from this seaweed using soxhlet extractor with chloroform:methanol (2:1) as solvent, while the composition of fatty acid was analysed using gas chromatrography accomplished by a mixture of 37 standard fatty acids (ST37AL85.D01). It was found that Ulva reticulata Forsskal contained (1,41±0,04)% (w/w) of crude lipids. The crude lipid was composed of 11 major fatty acids including pentadecanoic acid (12,08%), heptadecanoic acid (40,00%), cis-10-heptadecenoic acid (4,37%), oleic acid (1,77%), linolelaidic acid (4,31%), linoleic acid (13,01%), arachidic acid (4,71%), g-linolenic acid (2,27%), heneicosanoic acid (7,58%), cis-11,14-eicosedienoic acid (8,19%), and cis-8,11,14-eicosetrienoic acid (1,71%).

Co-Authors A A I Rahma Prabawanti A. A. Bawa Putra A. A. Tia Santika Dewi Anak Agung Istri Agung Mayun Laksmiwati Arisma Damayanti Ashri Rizki Hidayati Desak Putu Eka Nilakusmawati Dewa Ayu Indra Dewi Diah Prihatiningsih G. A. G. Indukirana Gusti Ayu Primandari Utami Haqqika Pasha Helen Helda Prastika I G. P. Sukmajaya A. P. T. I Gede Tangkas Mei Yasa I Gusti Putu Agus Ferry S.P. I K. D. Yasa I M, Sukadana I M. Sukadana I Made Dira Swantara I Made Oka Adi Parwata I Made Sudarsana I Nengah Simpen I Nyoman Suarsana I Nyoman Sumerta Miwada I Nyoman Widana I Wayan Karta I Wayan Pramana Eka Putra I Wayan Suirta I. A Raka Astiti Asih I. A. Gede Widihati I. A. R. Astiti Asih IDA AYU ASTARINI Ida Ayu Gede Widihati Ida Ayu Putu Sri Adnyasari Ida Bagus Putra Mahardika Ida Bagus Putra Manuaba Indriani Wisnu Susanto Panjaitan Irdhawati Irdhawati James Sibarani K. Sari K. Swandiyasa Ketut Ratnayani Ketut Ratnayani Komang Ardipa Saputra M. G. Agus Mandana Mahardika Aprilia Iflahah Manuntun Manurung N. K. Gumiati N. L. P. F. Widiari N. W. Bogoriani Ni G. A. M. Dwi Adhi Suastuti Ni Ketut Puspa Sari Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Mega Wahyuni Ni Luh Putu Putri Setianingsih Ni Luh Putu Suciptawati Ni Luh Rustini Ni Made Suaniti Ni Putu Adriani Astiti Ni Putu Diantariani Ni Putu Rahayu Kusuma Pratiwi Ni Putu Rusma Eva Arista Ni Putu Widayanti Ni Wayan Deswiniyanti Ni Wayan Oktarini A.C.Dewi Oka Ratnayani Oscar Yonathan Hambiyono P. P. Dewi Paramartha, I. D. G. Y. Putu Puspita Sari Putu Sariningsih Putu Suarya Riana Dyah Suryaningrum Riski Fatur Rachman Saeful Bahri Sakinatul Fatimah Simalango, N. T. H. Sri Rahayu Santi Sri Wahjuni Sri Wahjuni Tutut Hardikawati Wijaya, P. S. Wiwik Susana Rita WIWIK SUSANAH RITA Yenni Ciawi

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