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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Sewaka Bhakti Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Dharma Jnana
Ni Made Puspawati
Universitas Udayana
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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PENGARUH PEMBERIAN EKSTRAK METANOL DAUN TENGGULUN (Protium javanicum Burm f.) TERHADAP KADAR MALONDIALDEHIDA DAN AKTIVITAS SUPEROKSIDA DISMUTASE PADA DARAH TIKUS WISTAR YANG TERPAPAR ASAP ROKOK Puspawati, N. M.; Paramartha, I. D. G. Y.; Rustini, N. L.; Wijaya, P. S.
Jurnal Kimia (Journal of Chemistry) Vol. 18, No.1, Januari 2024
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2024.v18.i01.p05

Abstract

Asap rokok merupakan salah satu penyedia radikal bebas eksogen yang dapat menimbulkan stres oksidatif dan berpotensi menyebabkan penyakit degenerative. Tenggulun (Protium javanicum Burm. f.) merupakan salah satu tanaman yang berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak metanol daun tenggulun terhadap kadar malondialdehid (MDA) dan aktivitas superoksida dismutase (SOD) pada darah tikus Wistar yang dipapar asap rokok. Penelitian ini merupakan penelitian eksperimental dengan rancangan acak kelompok dengan posttest-only control group design. 24 ekor tikus putih jantan galur Wistar dibagi dalam 4 kelompok yaitu kelompok kontrol P0 (hanya diberi paparan asap rokok); kelompok perlakuan P1, P2, dan P3 (diberi ekstrak metanol daun tenggulun dengan dosis berturut-turut 50, 100, dan 200 mg/kgBB dan paparan asap rokok). Semua kelompok dipapar asap rokok dengan dosis 3 batang per hari selama 15 menit selama 14 hari. Pada hari ke-15, darah masing-masing tikus diambil untuk dianalisis kadar MDA dan aktivitas SOD. Hasil penelitian menunjukkan bahwa kelompok kontrol P0 memiliki kadar MDA tertinggi yaitu 6,73 ± 0,47 µM dan aktivitas SOD terendah yaitu 1,01 ± 0,12 ng/mL dibandingkan dengan kelompok perlakuan P1, P2, dan P3. Hal ini menunjukkan bahwa paparan asap rokok menyebabkan stress oksidatif. Pemberian ekstrak dengan dosis 50, 100, dan 200 mg/Kg BB secara signifikan (p<0,05) menurunkan kadar MDA dan meningkatkan aktivitas SOD jika dibandingkan dengan kontrol. Semakin tinggi dosis ekstrak yang diberikan, maka kadar MDA cenderung semakin menurun dan aktivitas SOD meningkat. Pemberian ekstrak metanol daun tenggulun dengan dosis 200mg/Kg BB (P3) memberikan hasil terbaik dengan kadar MDA terendah yaitu sebesar 2,91 ± 0,22 µM dan aktivitas SOD tertinggi sebesar 2,73 ± 0,23 ng/mL pada tikus Wistar yang terpapar asap rokok. Pada penelitian ini dapat disimpulkan bahwa ekstrak metanol daun tenggulun secara signifikan mampu menurunkan kadar MDA dan meningkatkan aktivitas SOD pada tikus yang terpapar asap rokok. Kata kunci: asap rokok, malondialdehida, Protium javanicum Burm.f, superoksida dismutase. ABSTRACT Cigarette smoke is a source of free radicals that trigger oxidative stress which can cause inflammation and lung damage. Tenggulun (Protium javanicum Burm. f.) is a plant that is known to have potential as an antioxidant. This study aims to determine the effect of giving tenggulun leaf methanol extract on malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity in the blood of Wistar rats exposed to cigarette smoke. This research is a randomized posttest-only control group design using 24 male white Wistar rats which were divided into 4 groups namely the control group (P0), only given exposure to cigarette smoke; the treatment group (P1; P2, and P3) were exposed to cigarette smoke and given 50, 100, and 200 mg/Kg BW of methanol extract of tenggulun leaves respectively. All groups were exposed to cigarette smoke at a dose of 3 cigarettes per day for 15 minutes for 14 days. On the 15th day, the blood of each rat was taken to analyze its MDA level and SOD activity. The results showed that the control group (P0) had the highest MDA level of 6.73 ± 0.47 µM and the lowest SOD activity of 1.01 ± 0.12 ng/mL compared to the treatment groups P1, P2, and P3. Administration of the extract at doses of 50, 100, and 200 mg/Kg BW significantly (p<0.05) reduced MDA levels and boosted SOD activity when compared to control. The higher the extract dose given, the levels of MDA reduced and SOD activity tends to increase. In this study, the administration of methanol leaves extract of tenggulun with a dose of 200mg/Kg BW (P3) on Wistar rats exposed to cigarette smoke gave the best results with MDA level of 2.91 ± 0.22 µM and SOD activity of 2.73 ± 0.23 ng/mL. The study concluded that the methanol extract of tenggulun leaves significantly reduced MDA levels and boosted SOD activity in rats exposed to cigarette smoke. Keywords: cigarette smoke, malondialdehyde Protium javanicum Burm.f, superoxide dismutase.
PEMANFAATAN EKSTRAK BUAH NAGA MERAH (HYLOCEREUS POLYRHIZUS) SEBAGAI PEWARNA ALAMI UNTUK MENINGKATKAN PRODUKSI PADA KELOMPOK JAJANAN TRADISIONAL DI DESA SANGGING KUSAMBA, KLUNGKUNG Wahjuni, Sri; Ida Bagus Putra Manuaba; Ni Made Puspawati; Ni Ketut Puspa Sari; Haqqika Pasha; Ashri Rizki Hidayati
JURNAL SEWAKA BHAKTI Vol 11 No 2 (2025): Sewaka Bhakti
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32795/3eh7zz71

Abstract

This community service activity aims to improve the quality and competitiveness of traditional snacks in Sangging Kusamba Village, Klungkung, by utilizing red dragon fruit (Hylocereus polyrhizus) pulp extract as a natural dye. Extraction was carried out using a maceration method using 90% ethanol. The characterization results using LC-MS Shimadzu LCMS-8040 showed the presence of betanidin pigments (2.28%), dopaxanthin-quinone (2.27%), valinebetaxanthin (2.12%), 3-O-methylquercetin (2.38%), kaempferol 3-O-galactoside (3.18%), kaempferol 7-O-D-glucopyranoside (2.40%), quercetin 3-rutinoside (2.18%), isorhamnetin 3-O-neohesperidoside (2.92%), betanin (0.94%), isobetanin (0.94%), and neobetanin (1.61%) were detected and contributed to the antioxidant activity of the extract. The presence of betacyanin and betaxanthin pigments plays a role in providing bright red-purple to orange colors, while flavonoid compounds (quercetin, kaempferol, isorhamnetin) and phenolics (ferulic acid, vanillic acid) support the antioxidant activity of the extract. Implementation in traditional snack business groups shows that natural dyes from red dragon fruit produce bright, stable colors, and are more in demand by consumers than synthetic dyes. Thus, the use of red dragon fruit extract can be a functional food solution that is safe, environmentally friendly, and has high economic value. These results indicate that dragon fruit flesh can be used effectively in this community service aims to develop natural dyes based on red dragon fruit peel as a safer alternative to synthetic dyes.
Co-Authors A A I Rahma Prabawanti A. A. Bawa Putra A. A. Tia Santika Dewi Anak Agung Istri Agung Mayun Laksmiwati Arisma Damayanti Ashri Rizki Hidayati Desak Putu Eka Nilakusmawati Dewa Ayu Indra Dewi Diah Prihatiningsih G. A. G. Indukirana Gusti Ayu Primandari Utami Haqqika Pasha Helen Helda Prastika I G. P. Sukmajaya A. P. T. I Gede Tangkas Mei Yasa I Gusti Putu Agus Ferry S.P. I K. D. Yasa I M, Sukadana I M. Sukadana I Made Dira Swantara I Made Oka Adi Parwata I Made Sudarsana I Nengah Simpen I Nyoman Suarsana I Nyoman Sumerta Miwada I Nyoman Widana I Wayan Karta I Wayan Pramana Eka Putra I Wayan Suirta I. A Raka Astiti Asih I. A. Gede Widihati I. A. R. Astiti Asih IDA AYU ASTARINI Ida Ayu Gede Widihati Ida Ayu Putu Sri Adnyasari Ida Bagus Putra Mahardika Ida Bagus Putra Manuaba Indriani Wisnu Susanto Panjaitan Irdhawati Irdhawati James Sibarani K. Sari K. Swandiyasa Ketut Ratnayani Ketut Ratnayani Komang Ardipa Saputra M. G. Agus Mandana Mahardika Aprilia Iflahah Manuntun Manurung N. K. Gumiati N. L. P. F. Widiari N. W. Bogoriani Ni G. A. M. Dwi Adhi Suastuti Ni Ketut Puspa Sari Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Mega Wahyuni Ni Luh Putu Putri Setianingsih Ni Luh Putu Suciptawati Ni Luh Rustini Ni Made Suaniti Ni Putu Adriani Astiti Ni Putu Diantariani Ni Putu Rahayu Kusuma Pratiwi Ni Putu Rusma Eva Arista Ni Putu Widayanti Ni Wayan Deswiniyanti Ni Wayan Oktarini A.C.Dewi Oka Ratnayani Oscar Yonathan Hambiyono P. P. Dewi Paramartha, I. D. G. Y. Putu Puspita Sari Putu Sariningsih Putu Suarya Riana Dyah Suryaningrum Riski Fatur Rachman Saeful Bahri Sakinatul Fatimah Simalango, N. T. H. Sri Rahayu Santi Sri Wahjuni Sri Wahjuni Tutut Hardikawati Wijaya, P. S. Wiwik Susana Rita WIWIK SUSANAH RITA Yenni Ciawi