Articles
ANALYSIS OF LIMONENE AND OTHER ANTIOXIDANTS IN COMMERCIALESSENTIAL OIL PRODUCTS COMPARED TO HOMEMADE VIRGIN COCONUT OIL
Ni Made Suaniti;
I Wayan Bandem Adnyana;
Manuntun Manurung;
Oka Ratnayani;
Raisyah Anjani
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)
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DOI: 10.24843/IJBB.2019.v07.i01.p02
Essential oil production in the market is very diverse with various brands labeled as antioxidants that have gained more attention in the society recently. The purpose of this study was to analyze limonene and other antioxidants content of the essential oils in the market compared to homemade virgin coconut oil. The method used was Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier-transform infrared (FTIR) Spectroscopy. The infrared spectrogram showed the presence of alkanes and esters respectively at wave numbers of 3000-2850 and 1750-1730 cm-1. Chromatogram results showed the presence of limonenecompounds and some antioxidants with a fairly good separation in the several types of oils, which was not detected in the homemade virgin coconut oil.
PENGETAHUAN DAN PEMAHAMAN DINI NARKOBA DAN ZAT ADITIF PADA GENERASI MUDA SEBAGAI ASET BANGSA DI DESA MENGWI BADUNG
N.M. Suaniti;
I N. Wirajana;
N.K. Ariati;
M. Manurung
Buletin Udayana Mengabdi Vol 17 No 4 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat
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DOI: 10.24843/BUM.2018.v17.i04.p11
Era globalisasi sebagai suatu peluang dan tantangan kaum muda Indonesia untuk berpartisipasi aktif memperoleh hal-hal yang tidak baik, salah satunya memahami pengetahuan awal tentang obat dan zat aditif yang memungkinkan dapat menyesatkan generasi muda. Drugs (narkotika dan obat / bahan berbahaya lainnya) dikenal sebagai obat-obatan yang disalahgunakan (narkotika, psikotropika dan zat adiktif lainnya) oleh Kementerian Kesehatan Republik Indonesia yang merujuk pada sekelompok senyawa yang umumnya memiliki risiko penambahan bagi pengguna. Selanjutnya, aditif makanan juga bisa menyebabkan keracunan. Badung adalah salah satu bagian dari dunia di mana tujuan wisata desa Mengwi menghubungkan daerah utara dan selatan menjadi rentan terhadap efek dari upaya pencegahan yang dilakukan sehingga salah satunya dengan memberikan pengetahuan obat-obatan dan aditif oleh otoritas yang relevan. Metode ini dilakukan dengan memberikan kuliah dan sesi tanya jawab dengan mengisi kuesioner pre dan posttest. Hasil kuesioner dianalisis dan menunjukkan peningkatan pemahaman obat dan aditif secara signifikan untuk menyebar secara merata pada generasi muda di desa Mengwi setelah kegiatan, tetapi kegiatan pendidikan ini perlu dilakukan secara berkala di setiap banjar dan diperluas di komunitas pedesaan lainnya di Bali.
PENGETAHUAN DAN PEMAHAMAN DINI NARKOBA DAN ZAT ADITIF PADA GENERASI MUDA SEBAGAI ASET BANGSA DI DESA MENGWI BADUNG
N.M. Suaniti;
I.N. Wirajana;
N.K. Ariati;
M. Manurung
Buletin Udayana Mengabdi Vol 16 No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat
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The era of globalization is an opportunity and challenges Indonesian youths to participate actively obtain in things that are not good, one of them understood early knowledge of drugs and additives that enable the younger generation can be misleading. Drugs (narcotics and drug/ hazardous materials) with another term drug (narcotics, psychotropic and other addictive substances) by the Ministry of Health of the Republic of Indonesia refers to a group of compounds generally have a risk of addition for users. Furthermore, food additives can also cause poisoning. Badung is one of part of the world where tourist destinations Mengwi village is connecting northern and southern regions become vulnerable to the effects of prevention efforts of prevention efforts undertaken so that one of them by giving lectures drugs and additives by the relevant authorities. The method is performed by giving a lecture and question and answer session with filling out the questionnaire pre and posttest. The results of the questionnaire were analyzed and showed an increase understanding of drugs and additives significantly to the spread evenly on the younger generation in Mengwi village after the activity, but this dedication activities need to be carried out periodically in each row and expanded in other rural communities in Bali
PENINGKATAN KUALITAS AIR BERSIH PEDESAAN DENGAN PENERAPAN SISTEM PENYARING AIR ALIRAN UP-FLOW PADA SISTEM AIR BERSIH DI DESA MENYALI
M. Suarda;
I G.N.O. Suputra;
N.M. Suaniti
Buletin Udayana Mengabdi Vol 18 No 2 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat
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DOI: 10.24843/BUM.2019.v18.i02.p25
Ketersediaan kuantitas dan kualitas air bersih masih menjadi permasalahan baik di daerah perkotaan maupun pedesaan. Seperti di desa Menyali kecamatan Sawan kabupaten Buleleng provinsi Bali sumber air bersihnya memanfaatkan rembesan air sawah. Kualitas sumber air tersebut umumnya belum memenuhi syarat kualitas baku mutu air bersih. Untuk itu dibutuhkan teknolgi penyaring air yang sederhana, mudah dan murah. Salah satu alternative solusi potensialnya adalah penerapan teknologi saringan pasir lambat. Seperti yang telah diterapkan dalam program KKN-PPM di desa Menyali, media penyaring menggunakan media ganda yaitu pasir silica sebagai media penyaring dan karbon aktif sebagai penghilang warna dan bau. Aliran air melewati media penyaring dirancang dari bawah ke atas (up-flow), dan proses pencucian media penyaring secara gravitasi tanpa menggunakan pompa air dan blower. Penyaring air baku (kotor) menjadi air bersih yang dibuat menggunakan media penyangga ijuk, media penyaring pasir silica tebal 30 cm dan karbon aktif tebal 40 cm. Hasil pengujian menunjukkan bahwa air bersih hasil penyaringan telah memenuhi baku mutu air kelas I sesuai yang dipersyaratkan dalam Peraturan Gubernur Bali nomor 16 tahun 2016. Namun belum memenuhi baku mutu air minum, sehingga air bersih tersebut harus dimasak sampai mendidih terlebih dahulu jika akan diminum. Kata kunci : Penyaring air, air bersih, saringan pasir lambat, pasir silica, carbon aktif, upflow
BIMBINGAN TEKNIS TEKNOLOGI PENGOLAHAN IKAN HASIL TANGKAPAN PADA KELOMPOK NELAYAN AMERTA SEGARA
I.W.B. Adnyana;
I.N. Suarnadwipa;
H. Wijaksana;
N.M. Suaniti
Buletin Udayana Mengabdi Vol 16 No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat
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Indonesia is an archipelago that is known as a maritime nation. Indonesia is rich in marine resources of fishery subsector. The majority of Indonesia's population are their livelihood as fishermen, especially in coastal areas. Bugbug village located in East Bali which is a coastal area that some community livelihood as fishermen. There are more than four groups of fishermen in this village, one of which is a fishermen group of Amerta Segara. The education level of their members are from elementary, middle and high school are dominated by primary school education. The number of members amounted to 25 persons. The encountered often problems on the fishermen is the fish handling a problem. When the harvest that the fish marketing become not good, the price is very cheap and fishermen's income decreases or not the maximum. Lack of knowledge about the fish processing cause fishermen's group is overwhelmed and being resigned on the situation. Seeing this condition, with our dedicated of team doing technical guidance on the fish processing technology to fishermen group of Amerta Segara on the Bugbug village, Karangasem District, Bali Province. The method is used the counseling and evaluation before and after counseling. The results obtained, that the community of the fishing groups of Amerta Segara very seriosly to listen the counseling and an increase in knowledge or understanding on the technologies presented.
Isoprostan Urin Sebagai Biomarka Keracunan Etanol dan Upaya Detoksikasinya dengan Ekstrak Etanol Kulit Buah Manggis (URINE ISOPROSTANE AS TOXIC BIOMARKER OF ETHANOL AND DETOXICATION EFFORTS BY USING ETHANOL EXTRACT OF SKIN MANGOSTEEN)
Ni Made Suaniti;
Manuntun Manurung
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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Isoprostane as a marker of oxidative stress which is a toxic metabolite of fatty acids, can be formedafter experiencing lipid peroxidation duo to ethanol. The aim of this study was to analyze the isoprostaneas early detection biomarker in urine of Wistar rats after ethanol consumption in sub-acute, followed byadministration of ethanol extract of rind of mangosteen fruit (EERMF). ELISA was use in this study fordiagnostic method as it can analyze the content of biological fluids even in small amount. Detection ofisoprostane compounds in the urine Wistar rats after ingestion of ethanol 20%v/v in sub acute was (0.8837± 0.0625) ng/mL decrease levels (0.4500 ± 0.0625) ng/ mL after administration EERMF 20% w/v. Isoprostancan be used as biomarker ethanol toxic and decreased level after giving EERMF.
Kerusakan Hati Akibat Keracunan Alkohol Berulang pada Tikus Wistar (LIVER DAMAGE DUE TO ALCOHOL INTOXICATION REPEAT IN WISTAR RATS)
Ni Made Suaniti;
Anak Agung Gede Sudewa Djelantik;
Ketut Suastika;
Nyoman Mantik Astawa
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
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The aims of this study was to determine the liver damage from alcohol intoxication in Wistar rats.The design used in this study was a randomized true experimental post test only control group design. Thestudy used 15 rats divided into 3 treatment groups each of which consists of 5 rats. The first group wasgiven distill water. The second group was given 5% alcohol, and the third group was given 20% alcohol. Ratswere treated with alcohol daily for six weeks. Biochemical markers were detected the levels of aldehydedehydrogenase (ALDH) in serum and histological changes in liver tissue. ALDH is a biochemical markerof a sensitive and specific ethanol after chronic alcohol administration. Blood sample was collected at 6and 24 hours after the last peroral administration of repeated alcohol treatment, and serum levels ofALDH was tested by enzyme linked immunosorbent assay (ELISA). The results showed that the levels ofALDH in the blood of alcohol treated Wistar rats significantly higher as compared to those of control rats.ALDH levels increased by 83.11% after administration of 5% alcohol and 112.05% after administration of20% taken after 6 hours of alcohol for 6 weeks. On samples taken after 24 hours, ALDH levels by 95.11%after administration of 5% alcohol and 86.79% after administration of 20% alcohol. Oral treatment with20% alcohol chronically was led to changes in the microscopic structure (necrosis) of liver tissue in Wistarrats. Liver tissue damage occured due to repeated use of alcohol is accompanied by increasing serum levelsof ALDH in Wistar rats.
ALDEHID DEHIDROGENASE DALAM TIKUS WISTAR SEBAGAI BIOMARKER AWAL KONSUMSI ALKOHOL SECARA AKUT
NI MADE SUANITI;
A.A.GEDE SUDEWA DJELANTIK;
I KETUT SUASTIKA;
I NYOMAN MANTIK ASTAWA
Jurnal Biologi Udayana Vol 15 No 1 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana
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Research on oral consumption of alcohol on rat Wistar was done to examine level of aldehyde dehydrogenase (ALDH) in rat serum. The research design was true randomized experimental post test only control group design. This research was conducted in two stages: first, eight rats were treated with 5% alcohol continuously for 1 week and as control aquadest was given to eight rats. The second stage was determination of ALDH levels. Wistar rat serum were taken after 6 hours and 24 hours of 5% alcohol consumption. The levels of ALDH increase by 117.15% after 6 hours of 5% alcohol consumption, while after 24 hours the levels of ALDH increase by 108.14%. ALDH levels in serum rat Wistar can be used as early biomarker of acute alcohol consumption.
GAMBARAN MIKROSKOPIS GINJAL TIKUS PUTIH (Rattus sp) JANTAN DEWASA SETELAH PEMBERIAN ETANOL KRONIS
Ayu Kasmita Dewi;
Ni Made Rai Suarni;
Ni Made Suaniti
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana
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Telah dilakukan penelitian dengan tujuan untuk mengetahui gambaran mikroskopis ginjal tikus putih (Rattus sp.) jantan dewasa setelah pemberian etanol kronis selama 48 hari. Delapan belas ekor tikus jantan dewasa dibagi ke dalam tiga kelompok secara acak. Pemberian alkohol diberikan secara oral (gavage) dengan dosis alkohol 10% dan 20% sebanyak 1ml/hari. Pada kelompok kontrol hanya diberikan aquades sebanyak 1ml/hari. Hasil penelitian menunjukkan bahwa alkohol 10% menyebabkan kerusakan pada ginjal seperti vakuolisasi lumen tubulus, pendarahan, dan infiltrasi sel radang yang bersifat fokal. Alkohol 20% menyebabkan kerusakan pada ginjal seperti infiltrasi sel radang bersifat multifokal (menyebar), pendarahan, vakuolisasi lumen tubulus, akumulasi sel debris dalam lumen tubulus, dan karyomegali. Variabel perubahan histopatologi ginjal yang diamati kemudian ditentukan tingkat kerusakannya dengan metode skor. Hasil yang diperoleh dianalisis dengan menggunakan uji ANOVA (analysis of variance) selanjutnya diuji dengan uji LSD. Dapat disimpulkan bahwa semakin meningkat konsentrasi alkohol yang diberikan secara oral (gavage) pada tikus putih jantan dewasa dapat meningkatkan kerusakan pada ginjal.
Komparasi senyawa volatil dalam serai wangi secara proximate analysis
Ni Made Suaniti;
I Wayan Bandem Adnyana;
Manuntun Manurung
Jurnal Energi Dan Manufaktur Vol 14 No 2 (2021)
Publisher : Department of Mechanical Engineering, University of Udayana
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DOI: 10.24843/JEM.2021.v14.i02.p05
Proximate as an initial analysis to detect the percentage of volatile compounds in citronella as an antioxidant and citronella waste can be made activated charcoal to purify waste such as used cooking oil. The purpose of this study was to compare the volatile compounds contained in citronella powder and charcoal. The analytical method used is Proximate Analysis. The lemongrass powder was put in an oven at 500oC for 24 hours to get charcoal. The test results showed that there was a decrease in the content of volatile compounds after making citronella charcoal by 46.88% in wet conditions and 61.02% in dry conditions. However, after citronella was added to virgin coconut oil (VCO) 10% w/w increased the volatile percentage to 100% for charcoal and 99.98% for powder with 0.9% moisture in both citronella.