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Epidemi Penyakit Antraknosa pada Tanaman Jeruk Nipis [Citrus aurantifolia (christm.) Swingle] di Desa Kertalangu, Kecamatan Denpasar Timur NI WAYAN SUNITI; I KETUT SUADA; I MADE SUDARMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.1, Januari 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Epidemic of Anthracnose Disease in the Lemon [Citrus aurantifolia (Christm.) Swingle] at Kertelangu Village, East Denpasar District           Anthracnose disease on lemon  was a newly discovered disease in Kertalangu Village, East Denpasar. Status of the disease unknown,. and lemon plants grown less well maintained and neglected, almost all plants showing symptoms of disease. Planting conditions like this it's possible anthracnose disease that can destroy rapidly growing cultivation of lemon. The research was conducted from April to June 2015 in the village of Kertalangu, district East Denpasar, Bali.           The results showed that the leaf spot disease found on lemon was anthracnose that caused by Colletotrichum gloeosporioides. Disease incidence of anthracnose was 26.16 – 35.00%, and disease severity was 17.38 – 24.10%, that is mean relatively mild disease. The infection of rate was  0.00128 – 0.0162 per leaf per day, its mean development of the disease was slowly.   Keywords: Anthracnose, Colletotrichum gloeosporioides, inhibiting ability, prevalence, endophytic and exophytic.  
Aktivitas In Vitro Anti Jamur Ekstrak Bulung Sangu Gracilaria sp. terhadap Jamur Patogen Fusarium solani (Mart) Sacc. Cabai Rawit ANDRIANI ANDRIANI; I GEDE PUTU WIRAWAN; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antifungal Activity of Gracilaria sp. against Pathogenic Fungi Fusarium solani (Mart) Sacc. Cayenne pepper The use of chemical pesticides as a control for cayenne pepper fusarium wilt caused by the Fusarium solani continuously for a long time can have a negative impact on the ecosystem, and toxic for humans. Seaweed Gracilaria sp. as a marine commodity that is abundant in Indonesia, especially in the Bali region, it contains bioactive compounds that have the potential to be developed as an antifungal. This study was aimed to determine the effectiveness of Gracilaria sp. in inhibiting the growth of Fusarium solani (Mart) Sacc. This research was a single factor experiment which was compiled based on a completely randomized design (CRD) consisting of six treatments of the concentration of Gracilaria sp. and each treatment was repeated 3 times. The treatment was given various concentrations of Gracilaria sp. in the fungus F. solani, the concentration is 0%; 0.5%; 1%; 1.5%; 2%; and 2.5%. Treatment was positive control (ketoconazole) and negative control (Tween 80). The results of this study indicate that the extract of Gracilaria sp. has antifungal activity that is fungistatic with weak inhibition against the fungus F. solani. MIC test results show that the minimum extract that can inhibit the growth of Fusarium solani is a concentration of 0.5%. MIC test results show that the minimum extract that can inhibit the growth of F. solani is concentration of 0.5% with an average diameter of 4 mm with a weak category. The highest inhibition power of Gracilaria sp. to F. solani in this study was 2.5% with inhibition of colony growth of 79.3%. The percentage of spore growth inhibition was 67.49%. The concentrations studied showed that the higher the extract concentration, the greater the inhibition power of Gracilaria sp. against F. solani.
Pengaruh Inokulasi Trichoderma sp. Indigenus terhadap Penyakit Akar Gada dan Pertumbuhan Tanaman Kubis (Brassica oleracea L.) NI PUTU MERRY SENI ANTARI; NI MADE PUSPAWATI; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Influence of Indigenous Trichoderma sp. Inoculation against Clubroot and the Growth of Cabbages (Brassica oleracea L.) The Cabbage (Brassica oleracea L.) is a plant that contains many vitamins and minerals needed by human. The problem which often experienced by cabbage farmers is clubroot deseases that caused by pathogenic Plasmodiophora brassicae Worr. Eco-friendly controls are needed so as not to adversely affect ecosystems by using natural enemies of the pathogen such as Trichoderma sp. The purpose of this research was to obtain the type Trichoderma sp. which effectively suppress clubroot and increase the growth of cabbage plants. The results showed that the most effective isolate was isolate from zucchini plant (Cucurbita pepo L.) rhizosphere (T1), followed by tomato plant (Lycopersicon esculentum L.) rhizosphere (T2), romana plant (Lactuca sativa L. var. longifolia) rhizosphere (T4), and from the spinach plant (Spinacia oleracea L.) rhizosphere (T9). The isolates were able to decrease clubroot and promote cabbage plant growth as well.
Uji Keefektifan Rizobakteri dalam Menghambat PertumbuhanJamurColletotrichum spp. Penyebab Antraknosa pada Cabai Merah (Capsicum annuum L.) NI LUH GEDE SRIYANTI; DEWA NGURAH SUPRAPTA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effectiveness of Rhizobacteria to Inhibit the Growth of Colletotrichum spp. the Cause of Antracnose on Red Chilli (Capsicum annuum L.) Colletotrichumspp. is one of pathogenic fungi that cause significant losses on red chilli. The use of rhizobacteria is considered as an alternative to control the growth of the fungus. The use of bio agent control is considered as selective and environmentally friendly. The aim of this study is to know the effectiveness of rhizobacteria and their filtrates in suppressing the growth of Colletotrichumspp. Results of this study showed that treatment with Pseudomonas fluorescens isolate TBZA on PDA medium could suppress the growth of Colletotrichumspp. by 94% when compared to control 14 days after inoculation. Treatment with filtrate of P. fluorescens isolate TBZA could suppress the anthracnose disease indicated by the lower disease incidence viz 32%. It is necessary to conduct further experiment under field condition to know the effectiveness of P. fluorescens isolate TBZA to suppress the growth of Colletotrichumspp. In addition isolation and identification of antifungal compounds in the filtrate is necessary to be done. Keywords: rhizobacteria, biologicalagents, and Colletotrichumspp.
Identifikasi Mikoriza Vesikular Arbuskular pada Rhizosfer Kopi Arabika (Coffea arabica L.) dan Kopi Robusta (Coffea robusta L.) dan Perbanyakannya dengan Media Zeolit PUTU AYU MEITA YUDIA DEWI; MADE SRITAMIN; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Vesicular Arbuscular Mycorrhizae Identification of Arabica Coffee(coffea arabica L.) and Robusta Coffee (Coffea robusta L.) Rhizosphere andIts Spore Multiplication in Zeolite Media.The high demand of coffee in the world led the farmers to improve coffee productivity by using inorganic fertilizers. Considering potential problems that may occured due to inorganic fertilizer use, aplication of biological fertilizer which one of them is containing Vesicular Arbuscular Mycorrhizae (VAM) could be expected to assist the growth of Arabica coffee and Robusta coffee plants in more naturally manner. This study aimed to determine the types mycorrhizal of rhizosphere coffee plants and determine the effectiveness of zeolite media and corn symbiont plant in propagation of VAM. The study began in April to August 2015. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated by root staining method and spores multiplication through trapping culture method. The results showed that VAM spores found in the rhizosphere arabica coffee plants are two genera VAM Acaulospora and Glomus, whereas in robusta coffee plant found three genera Acaulospora, Glomus, and Gigaspora. Mycorrhizal structures found in the rhizosphere of Arabica and Robusta coffee plants were arbuscular, vesicles, hyphae and inner spores. Corn symbionts plant and zeolite media were good combination for the propagation of VAM spores.
Pengendalian Penyakit Antraknosa (Colletotrichum gloeosporioides Penz)pada Buah Jeruk Siam (Citrus nobilis var. microcarpa) dengan Menggunakan Minyak Atsiri Cengkeh dan Sereh Dapur KADEK INTAN SURYANINGSIH; I MADE SUDANA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT   Anthracnose (Colletotrichum gloeosporioides Penz) DiseaseControlon “Siam”Orange (Citrus nobilis var. microcarpa) by UsingEssential Oilsof CloveandLemongrass The experimental designwas used Factorial Completely Randomized Design, with two  factors: FactorI : CloveEssential Oil with six treatmentstestedconcentration of 0.2%, 0.4%, 0.6%, 0.8%, 1% andwithoutconcentration. FactorII :LemongrassEssential Oil with six treatmentstestedconcentration of 0.2%, 0.4%, 0.6%, 0.8%, 1% andwithoutconcentration. The resultsshowedthat theessential oilof cloveandlemongrassconcentrations below1% cansuppressthe growth offungiC.gloeosporioides. The minimumconcentration ofcloveessential oiltreatmentsthat block100%is0,6% andlemongrassessential oilis0,4%. The effectiveness oflemongrassessential oilis higher thancloveessential oils.   Keywords: Orange,anthracnose, clove, lemongrass
Uji Efektivitas Ekstrak Daun Cengkeh (Syzygium Aromaticum L.) Terhadap Phytophthora Palmivora Penyebab Penyakit Busuk Buah Kakao (Theobroma Cacao L.) NI LUH PUTU SONIA SAVITA DEWI; DEWA NGURAH SUPRAPTA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effectiveness of Clove Leaf Extract (Syzygium aromaticum L.) againstPhytophthora palmivora the Cause of Cocoa Fruit Rot (Theobroma cacao L.) Cocoa fruit rot caused by Phytophthora palmivora is one of the main diseases that can greatly reduce the yield of cocoa production in the world. Therefore it isnecessary to control the disease in an environmentally friendly way. One of which isusing botanical pesticides. Clove leaf is one of the plants which extract can be usedas a fungicide. This study aimed to examine the effectiveness of clove leaf extract onP. palmivora the cause of cocoa fruit rot. MIC test and colony test was done using 10extract concentrations namely 0,1; 0,2; 0,3; 0,4; 0,5; 0,6; 0,7; 0,8; 0,9; 1,0 and 0,0%(control). The in vivo test uses 6 extract concentrations of 0,5; 0,6; 0,7; 0,8; 0,9; 1,0and 0,0% (control). The results showed the MIC (minimum inhibitory concentration)of clove leaf extract to suppress the growth of P. palmivora was 0.5% which means itis feasible to be used as a botanical pesticide. Under in vivo condition, clove leaf extractinhibited the growth of fungal colonies and infection and can be used as fungicide tosuppress the growth of P. palmivora. The extract concentration of 1% prevented thedamage of cocoa fruit caused by P. palmivora by 100%.
Pengamatan Gejala Penyakit CVPD pada 12 Spesies Tanaman Jeruk VEDERIS LEUNARDUS; I GEDE PUTU WIRAWAN; I KETUT SUADA; I GUSTI AYU DIAH YUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Diseases Symptoms Observation at 12 Species of Citrus Plants This study was conducted to observe symptoms that showed by citrus plantswhich already tested by PCR carry CVPDr DNA Fragment. CVPDr is resistance geneto prevent plant from CVPD diseases. Based on observations in the field showed thatfive from twelve species showed the symptoms even already carry the gene. Five ofthem are Citrus reticulata Selayer Buleleng, Citrus nobilis Buleleng, Citrus nobilisPecatu, Citrus nobilis Tabanan, dan Citrus reticulata Keprok Gianyar. Mutationanalysis and homology analysis should be done after this observation to know whythis gene is not functional to prevent CVPD diseases.
Analisis Fitokimia Ekstrak Bulung Boni (Caulerpa spp.) dan Uji Daya Hambatnya terhadap Fungi Fusarium moniliforme (Sacc.) Nirenberg VICKY TANDYA; I GEDE PUTU WIRAWAN; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Phytochemical Analysis Of Bulung Boni Extract (Caulerpa spp.) and Its Inhibiting Ability Against Fusarium moniliforme (Sacc.) Nirenberg Seaweed is a marine biota that produces bioactive compounds which are useful for the development of the pharmaceutical industry. Caulerpa spp. is a green algae that is commonly consumed as vegetables or fresh vegetables by tropical communities, but the use of Caulerpa spp. in other fields is not optimal. The purpose of this study was to determine the content of antifungal compounds in the extract of Caulerpa spp. and antifungal activity against F. moniliforme. The research method used was Gas Chromatography and the diffusion well method with 14 treatments and 3 replications. The design used was completely randomized with ANOVA analysis followed by Duncan's test with a confidence level of 5%. The results showed that the ethanol is extract of Caulerpa spp. has antifungal compounds, namely alkaloids, saponins, and organic acids which indicated fungistatic effect. Ethanol extract of Caulerpa spp. had minimal antifungal activity (MIC) against F. moniliforme at a concentration of 0.6% with an inhibition zone diameter of 5 mm and was categorized as weak. The best extract concentration in inhibiting F. moniliforme in the treattested was a concentration of 5% with an inhibition zone diameter of 13.67 mm and a percentage was 54.54% colony inhibition.
Uji Aktivitas Antimikroba Beberapa Ekstrak Bumbu Dapur terhadap Pertumbuhan Jamur Curvularia lunata (Wakk.) Boed. dan Aspergillus flavus LINK. IRMA SELVYANA Br. SITEPU; I KETUT SUADA; I GEDE KETUT SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol. 1, No. 2, Oktober 2012
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Antimicrobial Activity Test of Some Kitchen Seasoning Extracts on Growth of Fungus Curvularia lunata (Wakk.) Boed. and Aspergillus flavus LINK. This research was aimed to determine the ability of herbs extract in inhibiting the growth of fungus C. lunata and A. flavus and to determine the minimum inhibition concentration of each extracts as well. The results showed that the extracts could inhibit the growth of the two fungus. The most effective extract was turmeric against C. lunata and galangal extract against A. flavus with each inhibition was 38,6% and 26,6% respectively. The minimum inhibition concentration of all extracts were 0,5% on both C. lunata and A. flavus. Keyword : Antimicrobial, Kitchen seasoning, Curvularia lunata, Aspergillus flavus
Co-Authors A.A.A.A.S. Sunari A.A.N.G. Suwastika Aida Lousie Tenden Rompis Aleksey Stepanov Anak Agung Ngurah Gede Suwastika ANDRIANI ANDRIANI DELVIANA PANJAITAN Dewa Ayu Ari Febriyanti Dewa Ngurah Suprapta DWI SURYANTO EUSEBIO GOMES Fransen Sinaga GEDE WIJANA Gusti Ayu Yuniati Kencana HERLINDAH CHOIRI I Gede Ketut Susrama I GEDE PUTU WIRAWAN I GEDE SUSRAMA I Gusti Agung Ayu Suartini I Gusti Ayu Diah Yuniti I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I Kadek Ngestika Pradnyana I KADEK NGESTIKA PRADNYANA I MADE AGUS WIARTANA I Made Ary Putra Nata I Made Ary Putra Nata I Made Merdana I Made Parama Nanda Mahatya I MADE SUDANA I MADE SUDANA I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I Made Sukada I Nyoman Adi Khrisna Wijaya I NYOMAN MANTIK ASTAWA I NYOMAN RAI I Nyoman Suarsana I Nyoman Suartha I Putu Agus Santika Putra I WAYAN BUDIASA I WAYAN EKA ADI WIRAWAN I Wayan Suardana I WAYAN WIRAATMAJA I. A. P. Apsasri I. B. Ardana I.H. Utama I.W. Batan IDA AYU GEDE DIANGGI ADIATHY Ida Bagus Ngurah Swacita IRMA SELVYANA Br. SITEPU KADEK INTAN SURYANINGSIH Kadek Karang Agustina KETUT AYU YULIADHI M. Praborini M.D. Rudyanto MADE PHARMAWATI MADE SRITAMIN MARLIN MARHAENI PE N.W.S. Sutari Nataliya Shchegolkova NI KADEK LIA SWANDEWI Ni Komang Alit Astiari NI LUH GEDE SRIYANTI NI LUH PUTU SONIA SAVITA DEWI Ni Made Ayuratih Utami NI MADE PRITIYANTI DEWI Ni Made Puspawati Ni Made Savita Rasjman Rasjman NI MADE TRIGUNASIH NI NYOMAN ARI MAYADEWI NI PUTU LINDA SUNARIASIH NI PUTU MERRY SENI ANTARI Ni Putu Ratna Dewi Damayanti NI WAYAN SUNITI Oleg Gladkov Olga Yakimenko Onesimus Ke Lele PUTU AYU MEITA YUDIA DEWI Putu Ayu Sisyawati Putriningsih PUTU SUGITA Rodion Poloskin SANGGUL HUTASOIT SHEILA SIMANJUNTAK T. Sari Nindia TRISNA AGUNG PHABIOLA VEDERIS LEUNARDUS VICKY TANDYA WAYAN ADIARTAYASA