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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Caraka Tani: Journal of Sustainable Agriculture Nandur Advances in Tropical Biodiversity and Environmental Sciences
I Ketut Suada
Faculty Of Agriculture, Udayana University, Denpasar, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Performance Evaluation of Induced Mutagenesis using Colchicine and EMS Solution on Cowpea M3 Purple and Mung Bean Vima1 to Increase Resistance I Gede Ketut Susrama; Ni Made Trigunasih; I ketut Suada; Ni Nyoman Ari Mayadewi
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 2 (2022): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i2.56341

Abstract

Induced mutagenesis is one way to improve the quality of crops, especially to increase the resistance to pests and diseases. This research aimed to determine colchicine and ethyl methane sulfonate (EMS) mutagenesis on cowpea M3 purple and mung bean Vima1, in concatenation for developing resistant crops. This research consists of four packages: (1) first package (cowpea M3 purple seeds treated with colchicine solution, (2) second package (cowpea M3 purple seeds treated with EMS solution), (3) third package (mung bean Vima1 seeds treated with colchicine solution) and (4) fourth package (mung bean Vima1 seeds treated with EMS solution). The results of this research revealed that induced mutagenesis by colchicine solution treatment reduced the incidence of bean leaf beetles up to 19% on cowpea M3 purple, as well leaf miners by 5% to 9% and bean leaf beetles up to 5% on mung bean Vima1. Treatment of EMS solution decreased the incidence of bean leaf beetles by 17% on cowpea M3 purple and pink mealybug by 5% to 15% on mung bean Vima1. Induced mutagenesis using EMS solution significantly decreased vegetable leaf miner incidence by 33% to 93% or 71% on average. Colchicine and EMS solution treatment caused aphid attacks in cowpea M3 purple, particularly in pods. The attacks did not happen on leaves and aphid incidence in pods was slower than in control. Some promising mutant candidates were found from this research that will be used in further crop development studies. It is expected that the method and results of this research could inspire faster development of resistant crops.
PHYTOCHEMICAL ANALYSIS OF Mucuna pruriens L. LEAVES EXTRACT AND ITS INHIBITION TEST AGAINST Curvularia lunata (WAKK). BOED. THAT CAUSED LETTUCE LEAF SPOT I Made Ary Putra Nata; I Ketut Suada; Trisna Agung Phabiola
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p06

Abstract

Curvularia lunata is a pathogenic fungi that attack various plant species including lettuce. Its control needs to be carried out in an environmentally friendly manner, namely using botanical fungicides. M. pruriens is a legume plant that is widespread in Indonesia that has a potential as a botanical fungicide, beside as a traditional medicine. The purpose of this study was to study the chemical content of M. pruriens leaves and its potency to inhibit the growth of C. lunata. The determination of the compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the inhibition test was carried out using diffusion well, and agar dillution method. The result showed that the crude extract of M. pruriens leaves contained ethyl isothiocyanate that act as an antifungal with AUC of 20,78%. The MIC of the extract was 0,9%. The extract at 5% concentration was capable to inhibit C. lunata growth, mycelium mass, and conidia production respectively 38,33%; 67,14%; 99,88% compared to control.
Pollutants at Lake Buyan (Bali, Indonesia) and Solutions for Improving Its Environmental State Ni Made Savita Rasjman Rasjman; I Nyoman Adi Khrisna Wijaya; I Made Ary Putra Nata; Ni Putu Ratna Dewi Damayanti; I Wayan Budiasa; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lake Buyan in Buleleng, Bali, has various purposes, such as supplying water for crop and livestock farming and the domestic sector. Wastes generated by human activities in its immediate surroundings are increasing population pressures on the lake’s environment. According to Gubernatorial Regulation Number 16 of 2016, the lake’s water quality is in a poor state and is categorized as ‘polluted’. One of the ways proposed in this research to reduce the pollution level was to build a constructed wetland, which is a waste treatment system combining aquatic plants, soil or other growing media, and microbial assemblages. Results showed that 50 sets of vertical flow constructed wetlands with a single capacity of 40 m3 are needed around Lake Buyan. Another applicable design is concentrating wastewater flows through pipes into a 2000 m3 constructed wetland
Detection of Klebsiella spp. Causing Subclinical Mastitis in Sows on Farms in Puhu Village, Gianyar, Bali Ni Putu Deana Kanaya Sahrani; I Ketut Suada; Ni Nyoman Werdi Susari; Romy Muhammad Dary Mufa
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 3 (2025): October 2025
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i03.p04

Abstract

Subclinical mastitis is a disease that quite often affects sows during lactation without any apparent clinical symptoms, one of which is caused by Klebsiella Spp. The purpose of this study was to determine the quality of pig milk and whether Klebsiella spp. was detected in the pig milk. Thirty-four samples were tested using the California Mastitis Test (CMT) method, and samples positive for subclinical mastitis were further tested using MacConkey media. Additional tests were conducted, including the TSIA biochemical test and microscopic examinations of the growing bacterial isolates, to confirm the bacterial identity. The results of this study showed that 29 out of 34 positive samples had subclinical mastitis with different levels of severity. According to the laboratory results, the presence of Klebsiella spp (85%) bacteria was identified. The results of this study indicate that subclinical mastitis caused by Klebsiella spp remains a significant threat in pig farms in Puhu Village, Payangan Subdistrict, Gianyar Regency, particularly when cage sanitation and management are not optimal. Early detection and improved husbandry systems are needed to prevent similar incidents.  
Co-Authors A.A.A.A.S. Sunari A.A.N.G. Suwastika Aida Lousie Tenden Rompis Aleksey Stepanov Anak Agung Ngurah Gede Suwastika ANDRIANI ANDRIANI DELVIANA PANJAITAN Dewa Ayu Ari Febriyanti Dewa Ngurah Suprapta DWI SURYANTO EUSEBIO GOMES Fransen Sinaga GEDE WIJANA Gusti Ayu Yuniati Kencana HERLINDAH CHOIRI I Gede Ketut Susrama I GEDE PUTU WIRAWAN I GEDE SUSRAMA I Gusti Agung Ayu Suartini I Gusti Ayu Diah Yuniti I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I Kadek Ngestika Pradnyana I KADEK NGESTIKA PRADNYANA I MADE AGUS WIARTANA I Made Ary Putra Nata I Made Ary Putra Nata I Made Merdana I Made Parama Nanda Mahatya I MADE SUDANA I MADE SUDANA I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I Made Sukada I Nyoman Adi Khrisna Wijaya I NYOMAN MANTIK ASTAWA I NYOMAN RAI I Nyoman Suarsana I Nyoman Suartha I Putu Agus Santika Putra I WAYAN BUDIASA I WAYAN EKA ADI WIRAWAN I Wayan Suardana I WAYAN WIRAATMAJA I. A. P. Apsasri I. B. Ardana I.H. Utama I.W. Batan IDA AYU GEDE DIANGGI ADIATHY Ida Bagus Ngurah Swacita IRMA SELVYANA Br. SITEPU KADEK INTAN SURYANINGSIH Kadek Karang Agustina KETUT AYU YULIADHI M. Praborini M.D. Rudyanto MADE PHARMAWATI MADE SRITAMIN MARLIN MARHAENI PE N.W.S. Sutari Nataliya Shchegolkova NI KADEK LIA SWANDEWI NI LUH GEDE SRIYANTI NI LUH PUTU SONIA SAVITA DEWI Ni Made Ayuratih Utami NI MADE PRITIYANTI DEWI Ni Made Puspawati Ni Made Savita Rasjman Rasjman NI MADE TRIGUNASIH NI NYOMAN ARI MAYADEWI Ni Nyoman Werdi Susari Ni Putu Deana Kanaya Sahrani NI PUTU LINDA SUNARIASIH NI PUTU MERRY SENI ANTARI Ni Putu Ratna Dewi Damayanti NI WAYAN SUNITI Oleg Gladkov Olga Yakimenko Onesimus Ke Lele PUTU AYU MEITA YUDIA DEWI Putu Ayu Sisyawati Putriningsih PUTU SUGITA Rodion Poloskin Romy Muhammad Dary Mufa SANGGUL HUTASOIT SHEILA SIMANJUNTAK T. Sari Nindia TRISNA AGUNG PHABIOLA VEDERIS LEUNARDUS VICKY TANDYA WAYAN ADIARTAYASA