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All Journal Sainstek Jambura Journal of Food Technology Jambura Nursing Journal Jurnal Pengabdian pada Masyarakat Kepulauan Lahan Kering Jurnal Kolaboratif Sains Journal of Language and Health Zaitun :Jurnal Ilmu Kesehatan Indonesian Red Crescent Humanitarian Journal Jurnal Pengabdian Masyarakat Farmasi : Pharmacare Society Medical and Health Journal Jambura Medical and Health Science Journal Medic Nutricia : Jurnal Ilmu Kesehatan Damhil: Jurnal Pengabdian kepada Masyarakat
Zuhriana K.Yusuf
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Sainstek

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POLYMERASE CHAIN REACTION (PCR) K.Yusuf, Zuhriana
Sainstek Vol 5, No 2, 2010
Publisher : Sainstek

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Abstract

(Polymerase Chain Reaction, PCR) adalah suatu metode enzimatis untuk amplifikasi DNA dengan cara in vitro. Pada proses PCR diperlukan beberapa komponen utama, yaitu DNA cetakan, Oligonukleotida primer, Deoksiribonukelotida trifosfat (dNTP), Enzim DNA Polimerase, dan Komponen pendukung lain adalah senyawa buffer. Pada proses PCR menggunakan menggunakan alat termosiklus. Sebuah mesin yang memiliki kemampuan untuk memanaskan sekaligus mendinginkan tabung reaksi dan mengatur temperatur untuk tiap tahapan reaksi. Ada tiga tahapan penting dalam proses PCR yang selalu terulang dalam 30-40 siklus dan berlangsung dengn cepat yaitu denaturasi, anneling, dan pemanjangan untai DNA. Produk PCR dapat diidentifikasi melalui ukurannya dengan menggunakan elektroforesis gel agarosa. Teknik PCR dapat dimodifikasi ke dalam beberapa jenis diantaranya : PCR- RFLP, PCR RAPD, nested- PCR,Quantitative- PCR, RT- PCR dan inverse PCR. Keunggulan PCR dikatakan sangat tinggi. Hal ini didasarkan atas spesifitas, efisiensi dan keakuratannya. Kata kunci : PCR
Analisis RAPD ( Random Amplified Polymorphic DNA)untuk Diferensiasi Mycobacteriun tuberculosis isolat klinik sensitif INH dan Rifampisin di Makassar Yusuf, Zuhriana K.
Sainstek Vol5, No 1, 2010
Publisher : Jurnal Sainstek

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Abstract

This study aims to find out: (1) whether there is a genetic diversity on M.tuberculosis isolat clinic that is sensitif to INH dan rifampisin with RAPD method, and (2) the description of its polimorfism diferentiation. The research samples include 5 Mycobacteriun tuberculosis isolate that is sensitive to INH and rifampisin. The study includes DNA extraction using Wizard Genom DNA purification method, amplification using 5 primer: A-2 (5 TGCCGAGTCG 3 , 70% G+C ), OPN-09 ( 5TGCCGGCTTG 3, 70% G+C), N-9 (5 TGCCGGCTTG 3, 70% G+C), BG-66 (5CGACGCTGCG 3, 80% G+C) , 80% G+C), U-19 ( 5 GTCAGTGCGG 3, 70% G+C), and electroforesis. The isolat genetic diversity was analysed by using Dendro Unweighted Pair Group Method of Aritmethic (UPGMA) : A Dendogram Construction Utility method from Dr. Santi GarciaValive/2009. The results show that there is a genetic diversity on the 5 isolate Mycobacterium tuberculosis that are sensitive to INH and rifampisin, amplified with 5 primer. The size of amplified DNA bands is between 200-1100bp. The genetic variation can be seen in the number of fragments, the size of fragments and the number of polimorfik bands. The polimorfik percentage of the isolate is between 66.67% - 100%, or 80.28 in average. The UPGMA analysis results in a dendogram with a group cooficient of 0 40% or a diversity of 60 100%. Keywords: RAPD, Mycobacterium tuberculosis, sensitive to INH and rifampisin.
Co-Authors Abdul, Romy Ali, Nabila Salsabila H. Antu, Yuniarty Busura, Fazrab Erwin Purwanto Gobel, Muhammad Rafliansyah Haji Jafar, Cindy Puspita Sari Handayani, Dwi Lutvia Hasan, Zahwanabila Ramadhanti Hunawa, Rachmawaty Dirham Ibrahim, Nurani Ilato, Khairurrijal Irsha Karim, Cecy Rahma Kasim, Vivien Novarina Liputo, Gusti Pandi Moh. Nisyar Sy. Abd. Azis mulyadi, jesica - Nagaring, Sulaiman Putra Nanang Roswita Paramata Nirwanto K. Rahim Nurdiana Djamaluddin Nurlin Nurlin, Nurlin Polapa, Sitti Muthmainnah Nur Putri Ayuningtias Mahdang Rahma, St Richard Arie Monoarfa Sitti Rahma, Sitti Sri Manovita Pateda Suleman Ibrahim Vivien Novarina Kasim Wahjudi, Chairul Zulkifli B. Pomalango