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All Journal Media Veteriner Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Veteriner Nusantara Journal of Veterinary and Animal Sciences Buletin Veteriner Udayana
I Gusti Ketut Suarjana
Laboratorium Bakteriologi Dan Mikologi Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Veteriner

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Analisis Gen Patogenik iroN Escherichia coli Penyebab Kolibasilosis pada Ayam Kampung Tania Ria Gunawan; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian colibacillosis is an infectious disease caused by avian pathogenic Escherichia coli (APEC). APEC ability to cause disease depends on many pathogenic factors, one of them is iroN pathogenic gene. This study purposed to find out iroN gene sequence in APEC in Bali. Two APEC isolates from free range chicken in Tabanan dan Badung has been used. The isolates have been been purified and were available at Laboratory of Veterinary Bacteriology, Faculty of Veterinary Medicine, Udayana University. Chelex 10%was used for DNA isolation. DNA amplification using published DNA primer has been conducted with polymerase chain reaction (PCR) method. The PCR product was sequenced at First Base Laboratories, Malaysia using Sanger’s Dideoxy Nucleotide Termination method. The iroN gene of both isolates can be analyzed and have 659 bp in length. Both were 100% homologous. Phylogenic test using 52 DNA sequence of iroN gene from Escherichia coli and other bacteria in the world was conducted in MEGA 5.2. All data have 24 polymorphic sites of nucleotide acid and eight polymorphic sites of amino acid. The iroN gene of Bali isolates was in the same group as iroN gene from Australia (MF174860) and Hong Kong (MF474175). This gene can be used as pathogenic marker of APEC in Indonesia.
Co-Authors Agnes Endang Tri H Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Alya Nita Shena Gayanti Anak Agung Gede Agung Ananta Kusuma Anak Agung Sagung Massita Jenika Putri ANITA KAROLINA SIMAMORA Ashley BERATA BERATA Bianca Violanda Junus Bolla, Nelci Elizabeth chyntia nirmalasari mantrawan Daniel Opristanta Barus Danu Suprayogo Dwi Arso Purba Dwi Astalia Zuanita Eggy Hidta Lusandika Elviana, Dina Fanayoni, Aditana Genta Dhamara Adam Putranto Gusti Ayu Yuniati Kencana Hapsari Mahatmi HERTATI ANRIANI LUBIS I Gede Eka Chandrawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Kadek Juli Handriana I Ketut Berata I Ketut Berata I Ketut Suada I Ketut Tono Pasek Gelgel I Made Adhi Kusuma Dwipayana I Made Kardena I Made Merdana I Made Sukada I Nengah Kerta Besung I Nengah Kerta Besung I Nengah Kerta Besung I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I Putu Cahyadi Putra, I Putu Cahyadi I Putu Yasmanta Primarta Putra I Wayan Aris Suharsa I Wayan Suarnata I.A.P. Apsari IB. KADE SUARDANA Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Made Oka Ida Bagus Oka Winaya Ike Siwi Widyaningtyas Indra Manik Pradipta Indra, Rusmin Iwan Harjono Utama JERRY BIROWO K. Tono P.G Kadek Andre Sulaksana Kadek Satria Adi Marhendra KARTINI HUTASOIT Ketut Tono Pasek Gelgel Leny Beatry Veronica Luh Made Sudimartini M Hasan Isnan Made Deddy Dharmana Putra Made Tubagus Dharmajaya Magfirah Syahruddin Makselina Ayu Dwi Purwanti MARGARET ANASTASIA MANULLANG Marlissa, Faccettarial Cylon Marchel MAS DJOKO RUDYANTO Matilda Krisnawati Mochammad Imron Awalludin MUHAMMAD RHIYAN SAKTI N.K. Suwi N.N. Suryani Nazara, Agustina Lesmauli Ni Kadek Meita Swandewi Ni Kadek Puspa Dewi Ni Ketut Suwiti Ni Putu Nicky Mirahsanti Nyoman Anandiya Ramaditya P. Suastika Paramita, Putu Wahyuni Pratanto, Aditya Putri Udayani Putri, Rindar Mentari Nusanti Putu Henrywaesa Sudipa Putu Henrywaesa Sudipa Ratna Pandu Finata Septian Naria Rusmaniarno Sousa, Rojelio Dias Trindade Tania Ria Gunawan Trisno, Komang Voni Cornelia Br Sembiring Wati, Ni Komang Desi Yena Widya Asmara Yuniarti Sasmita Yusmaniar Galuh Adi Luhung