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Journal : IJOBI

Identification and Mercury Sensitivity Test of Rhizosphere Bacteria From Ageratum conyzoides Plants Growing In Ratatotok Gold Mine, North Sulawesi Rania Nur Farida; Anna Rakhmawati; Tien Aminatun; Sri Atun
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 2 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i2.933

Abstract

Indonesia is known for its abundant natural resources, such as gold. However, the mercury-based amalgamation method in illegal gold mining causes environmental pollution. This research explores the potential of utilizing mercury-resistant rhizosphere bacteria from Ageratum conyzoides, a gold hyperaccumulator plant, to develop an effective bioremediation strategy for contaminated gold mining areas. This descriptive-exploratory study aims to identify and determine the sensitivity of mercury in five rhizosphere bacteria isolates from previous research. Bacteria were isolated from the roots of Ageratum conyzoides growing in the Ratatotok gold mining area of North Sulawesi. Bacteria identification was determined using profile-matching methods based on microscopic, macroscopic, physiological, and biochemical features. Similarity Indexes were determined using dendrogram visualization with MVSP software. Mercury sensitivity was analyzed using the cup-plate technique, varying mercury concentrations from 0 ppm to concentrations where clear zones appeared, indicating bacterial growth inhibition. The result indicated that the five isolates were bacteria from the genera Alcaligenes, Pseudomonas, Enterococcus, Streptococcus, and Micrococcus. Notably, Pseudomonas showed the highest potential for mercury resistance, being mercury sensitive at a concentration of 470 ppm, followed by Alcaligenes (110 ppm), Enterococcus (30 ppm), and both Streptococcus and Micrococcus (20 ppm).
Characterization And Identification of Halophilic Bacteria (Rastrelliger sp.) Extracellular Lipase Generator in Beringharjo Market Octavia, Bernadetta; Rakhmawati, Anna
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 2 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i2.1056

Abstract

Advances in biotechnology have allowed for the widespread use of lipase enzymes. One of the producers of lipase enzymes is halophilic bacteria. This study aims to determine the characteristics and genus, as well as the kinship relationship between lipase-producing halophilic bacterial isolates from salted fish (Rastrelliger sp.) in Beringharjo Market, Yogyakarta. Salted fish samples were isolated on Nutrient Agar + 3% NaCl + Tween 80 + 0.1% Rhodamine-B selective media to grow lipase-producing halophilic bacteria. Identification was carried out by the matching profile method based on Bergey's Manual of Determinative Bacteriology. The kinship relationship between bacterial isolates was classified using MVSP 3.1 software with the UPGMA clustering algorithm and constructed in the form of a dendogram.
Exploration and Identification of Lichen in the Temon, Depok, and Tegalrejo Hafizh Adyatma; Anna Rakhmawati; Bernadetta Octavia
Indonesian Journal of Bioscience (IJOBI) Vol. 3 No. 2 (2025): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v3i2.2484

Abstract

This research aims to identify the diversity of lichen species in three different areas: Temon, Kulon Progo; Depok, Sleman; and Tegalrejo, Magelang. Besides that to analyze their ecological role as environmental bioindicators. The method used was direct field observation with visual documentation using a mobile phone camera, followed by morphological identification. The results of the study successfully identified nine lichen species: Flavoparmelia caperata, Physcia atrostriata, Dirinaria applanata, Phlyctis argena, Acarospora socialis, Ropalospora viridis, Pyxine sorediata, Cryptothecia striata, and Graphis sp.. These species exhibited unique adaptations to various substrates (tree bark, rocks, and urban environments) and showed sensitivity to environmental changes, particularly air pollution. The mutualistic symbiosis between fungi and photobionts (algae/cyanobacteria) is key to the resilience of lichens in marginal habitats. These findings strengthen the potential of lichens as natural bioindicators and ecological pioneers, while also highlighting the need for further research to map the distribution and applications of lichens in Indonesia.