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Efektivitas Limbah Kulit Kering Nanas Madu (Ananas Comosus l.Merr) untuk Pembuatan Bioetanol dengan Proses Fermentasi dan Distilasi E Hugeng Wandono; Endang Kusdiyantini; Hadiyanto Hadiyanto
Jurnal Energi Baru dan Terbarukan Vol 1, No 2 (2020): Juli 2020
Publisher : Program Studi Magister Energi, Sekolah Pascasarjana, Universitas Diponegoro, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.347 KB) | DOI: 10.14710/jebt.2020.9019

Abstract

Bioenergi adalah salah satu energi yang dapat diperbaruhi dan juga ramah lingkungan, salah satunya adalah bioetanol. Bahan baku yang mengandung glukosa, selulosa dan pati dapat digunakan menjadi bioetanol Nanas madu adalah tanaman yang dapat dimanfaatkan menjadi bioenergi. Penelitian ini bertujuan membuktikan efektivitas limbah kulit nanas madu kering untuk pembuatan bioetanol serta mengetahui kadar bioetanol yang dihasilkan. Proses fermentasi yang digunakan dalam peneltian ini . Kulit nanas madu yang sudah dikeringkan di proses hidrolisis, fermentasi dan distilasi. Proses fermentasi dengan variasi waktu fermentasi 1 hari, 2 hari, 3 hari, 4 hari, 5 hari, 6 hari, 7 hari,8 hari, 9 hari, 10 hari, 11 hari dan 12 hari. Hasil fermentasi tercepat akan didistilasi sebanyak dua kali dan dianalisa kadar bioetanol dengan GC-MS. Kadar bioetanol dari limbah kulit kering nanas madu didapatkan sebesar 95,66 % dengan waktu fermentasi selama 4 hari.
Isolasi dan identifikasi bakteri asam laktat dari pangan fermentasi berbasis ikan (Inasua) yang diperjualbelikan di Maluku-Indonesia Adde Lolita Putri; Endang Kusdiyantini
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (669.692 KB) | DOI: 10.14710/jbt.1.2.6-12

Abstract

Lactic acid bacteria are beneficial bacteria for the health of the body by improving the balance of the intestinal microflora. Lactic acid bacteria can generally be isolated from fermented foods, fruits, vegetables and meat. Inasua is a traditional fermented fish product originating from Central Maluku (Teon, Nila and Serua islands) wherein the fermentation process is carried out at room temperature for a certain time. The purpose of this study was isolation and identification based on the morphological characteristics of lactic acid bacteria in inasua. Isolation of Lactic Acid Bacteria was carried out using the pour plate method and the streak method on the media de Man, Rogosa and Sharpe Agar (MRSA) and Nutrient Agar media. The culture was incubated at 370C for 48 hours. The growing colonies were observed for morphological characteristics and GRAM staining of bacteria was carried out. The results of isolation of BAL from inasua in the media de Man, Ragosa and Sharpe Agar (MRSA) + CaCO3 obtained 4 bacterial isolates, namely INS-A1, INS-A2, INS-A3 and INS-A4 and only 2 isolates that have the characteristics of positive GRAM with Bacillus forms, namely INS-A2 and INS-A4. It is suspected that the 2 isolates were isolates of Lactic Acid Bacteria. The results of isolation on Nutrient Agar (NA) media contained 5 isolates namely INS-B1, INS-B2, INS B3, INS-B4, INS-B5 which have characteristics as negative GRAM shaped Bacillus
Karakterisasi morfologi, biokimia, dan uji enzimatis isolat khamir buah apel (Malus domestica Borkh.) yang berpotensi menghasilkan bioetanol Hilmi Fadhli; Endang Kusdiyantini; Nurhayati Nurhayati
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1267.955 KB) | DOI: 10.14710/jbt.2.2.62-73

Abstract

Bioethanol can be produced by the fermentation using microorganisms such as yeast. The type of yeast that can produce bioethanol is still limited to certain yeast types such as Saccharomyces cerevisiae. Isolation and characterization of yeast need to be done to determine yeast isolates capable of producing bioethanol. Yeast can grow on a substrate that is rich in sugar as in the apple fruit. Enzymatic tests can support the ability to use on various substrates. This study aims to isolation, characterization, and enzymatic tests of yeast from apple that potential to produce bioethanol based on morphological and biochemical tests. The isolation method is done by using spread plates on PDA media. Morphological tests are carried out by making macroscopic (form, color, elevation, margin, the texture of colonies) and microscopic (form, budding, size of cells)observations of colonies and yeast cells. The growth was carried out on PDA at 6, 28, 37ᵒC, and also 50% glucose observed, Carbohydrate fermentation test (glucose, maltose, sucrose, lactose), and urease were used to the biochemicals characterization. Enzymatic tests were using amylase test, protease test, lipase test, and cellulase test. The bioethanol was measured bypycnometer method. The results of yeast isolation from apples were obtained 3 isolates, namely K1, K2, K3 with cell size 3,74 µm, 3,79 µm, and 3,41 µm respectively. Enzymatic test results showed that the presence of amylase enzyme activity in yeast. Based on morphological and biochemical characterization, K2 isolate was selected as genus Pichia sp. and able to producebioethanol by 5.6% (v / v).
Pengaruh variasi sumber karbon terhadap aktivitas enzim isolat khamir Ep A dari limbah kulit buah nanas madu (Ananas comocus L.) Evi F Simanjuntak; Endang Kusdiyantini; Arina Tri Lunggani
NICHE Journal of Tropical Biology Vol. 4, No. 1, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.1.1-7

Abstract

Deteksi Gamma-Aminobutyric Acid (GABA) pada bakteri asam laktat hasil isolasi produk fermentasi petis ikan dari Rembang Agni Rizqy Berliyanti; Agung Suprihadi; Endang Kusdiyantini
NICHE Journal of Tropical Biology Vol. 3, No. 2, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.2.59-67

Abstract

Respon Penurunan Kadar Glukosa Darah Tikus (Rattus norvegicus) Hiperglikemik Setelah Pemberian Berbagai Minyak Konsumsi Enny Yusuf Wachidah Yuniwarti; Tyas Rini Saraswati; Endang Kusdiyantini
Buletin Anatomi dan Fisiologi Volume 3, Nomor 2, Tahun 2018
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.328 KB) | DOI: 10.14710/baf.3.2.2018.146-149

Abstract

Olive oil, VCO, dan minyak buah merah memiliki manfaat sebagai antidiabetik karena ketiga minyak tersebut mengandung antioksidan yang tinggi. Penelitian ini bertujuan untuk membandingkan respon ketiga minyak tersebut terhadap persentase penurunan kadar glukosa darah, berat badan dan diameter sel hepatosit tikus jantan (Rattus norvegicus) hiperglikemik. Lima belas ekor tikus putih (Rattus norvegicus) jantan dengan umur sekitar 2 bulan digunakan sebagai hewan uji. Induksi aloksan sebesar 150 mg per kg BB tikus secara intraperitonial dilakukan untuk mendapatkan tikus hiperglikemik. Design penelitian menggunakan Rancangan Acak Lengkap, dengan tiga perlakuan dan lima ulangan, yaitu P1 merupakan  perlakuan dengan VCO 0,2 ml per BB tikus, P2 merupakan perlakuan dengan olive oil 0,2 ml per BB serta P3 merupakan perlakuan dengan minyak buah merah sebanyak 0,2 ml per BB tikus per hari. Perlakuan diberikan secara oral selama empat minggu, data yang didapat dianalisa dengan ANOVA dan uji lanjut dengan uji LSD. Hasil yang didapat menunjukkan bahwa rerata persentase penurunan kadar glukosa darah dan bobot badan pada P1 berbeda nyata dengan P2 dan P3 (p<0.05), sedang diameter sel hepatosit tidak menunjukkan perbedaan yang nyata antar perlakuan (p>0.05), sehingga dapat disimpulkan bahwa VCO lebih potensial sebagai antidiabetik dibanding olive oil dan minyak buah merah,
Aktivitas Antioksidan Berbagai Minyak Edible Menggunakan Metode DPPH Enny Yusuf Wachidah Yuniwarti; Tyas Rini Saraswati; Endang Kusdiyantini
Buletin Anatomi dan Fisiologi Volume 3, Nomor 1, Tahun 2018
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.3.1.2018.85-88

Abstract

Natural antioksidan secara tradisonal sudah digunakan untuk mencegah terjadinya penyakit dan juga digunakan untuk mengobati berbagai penyakit, terutama penyakit degeneratif. Indonesia mempunyai bahan alami yaitu berbagai minyak edible yang digunakan sebagai nutraceutical atau natural antioksidan. Penelitian ini bertujuan untuk mengkaji aktivitas antioksidan dari minyak buah merah, olive oil, vco dan minyak wijen, menggunakan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH), sehingga didapatkan jenis minyak edibel yang dapat digunakan sebagai natural antioksidan. Radikal bebas  1,1-diphenyl-2-picrylhydrazyl (DPPH) digunakan sebagai salah satu metode dalam pengukuran aktivitas antioksidan. Aktivitas scavenging DPPH melalui delokalisasi elektron yang akan mengubah larutan dari kuning menjadi ungu. Metode DPPH ini merupakan metode yang sensitif untuk menguji aktivitas antioksidan Penelitian ini menggunakan empat jenis minyak edible dengan lima kali ulangan pengukuruan. Berdasarkan hasil ANOVA dan uji lanjut BNT pada taraf kepercayaan 95% maka didapatkan bahwa olive oil merupakan antioksidan yang paling kuat. Kata kunci : olive oil; vco; red fruit oil; natural antioksidan
Pigment Production of Monascus sp. Isolated from Angkak in Semarang Region, Central Java, Indonesia Endang Kusdiyantini; Soni Nugraha; Arina Lunggani
Indonesian Food and Nutrition Progress Vol 14, No 1 (2017)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.24256

Abstract

Angkak (red fungi rice, red yeast rice) is a traditional fermentation by fungi Monascus in a lot of Asia Countries, which is useful as a natural food colorant.  The aim of this research was to obtain pure isolate of red fungus from angkak in Semarang. The growth and the red pigment production of the selected isolate in the different initial medium pH and nitrogen sources were observed. The treatment was done by growing the isolate in PDB (Potato Dextrose Broth) medium at initial pH 3, 5, 7 and 9 and as well as optimization of nitrogen sources with 1% ammonium chloride, 1% ammonium nitrate, and 1% peptone. Analysis of the fungi growth was performed by weighing mycelia biomass using dry weight method and the red pigments were analysis using spectrophotometer at a wavelength (λmax) of 500 nm. The results showed that the highest absorbance value of pigment (0.81) was obtained at pH 7, and the highest value of the cell dry weight was also obtained at pH 7 (1.23 g/L). The results showed that 1% ammonium chloride was the best nitrogen source for absorbance of red pigment production (0.82), but the highest of dry weight (2.56 g/L) was obtained by using medium with 1% ammonium nitrate as a nitrogen source.
Kempong, A Traditional Fermented Food in Karangpucung Kidul village, Linggapura Bumiayu, Central Java: Fermentation Agent and Their Roles Isworo Rukmi; Devia Kusmawati Arfina; Endang Kusdiyantini
Indonesian Food and Nutrition Progress Vol 14, No 1 (2017)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.24257

Abstract

Kempong is a traditional fermented food that is found only in South Karangpucung    Linggapura Bumiayu village, Central Java. It is prepared from palm kernel cake (PKC).  This fermented food is consumed mostly everyday by the people in the village as a side dish or snack. Study on the mold important in the kempong fermentation was done by isolating molds from kempong, PKC, laru and the air of preparation room. Eleven species of molds were successfully isolated from different samples. Among the mold isolates three species came out from kempong product, i.e. R. oryzae, E. chevaliery and A. oryzae. The isolates showed the capability in producing amylase, protease, lipase, and cellulase. Both R. oryzae and A. oryzae indicated as the main fermentation mold in kempong production, because the inoculum laru only contained these two species.
Peningkatan kualitas air Waduk Diponegoro Semarang dengan filter arang aktif Nia Fadlilatul Laily; Nurhayati Nurhayati; Endang Kusdiyantini; Arina Tri Lunggani
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.48195

Abstract

Air bersih harus memiliki kualitas yang baik dari segi fisika, kimia, maupun biologisnya. Waduk Diponegoro mempunyai peran strategis dalam pengelolaan sumber daya air Kota Semarang. Berdasarkan hasil survei pada Waduk Diponegoro. Tujuan penelitian untuk meningkatkan kualitas air Waduk Diponegoro berdasarkan kategori kelas indeks kualitas air parameter biologi, fisika, dan kimia dengan perlakuan filter arang aktif. Arang aktif banyak digunakan untuk menghilangkan kontaminan dalam air karena kapasitasnya yang tinggi untuk adsorpsi senyawa tersebut, yang timbul dari luas permukaan dan porositasnya yang besar serta dapat digunakan untuk mengurangi keberadaan E. coli pada air. Proses uji bakteriologis air dilakukan dengan menggunakan metode deteksi bakteri dengan 3M Petrifilm E.coli/Coliform. Metode yang digunakan pada penelitian meliputi sterilisasi alat, pengambilan sampel air pada titik inlet dan outlet, filtrasi air dengan ketebalan arang aktif 10, 15, dan 20 cm, inokulasi sampel air, perhitungan jumlah koloni, pengukuran dan penentuan kualitas air. Hasil penelitian menunjukkan bahwa semakin tebal media arang aktif yang digunakan semakin baik kualitas air yang dihasilkan. Perlakuan filtrasi arang aktif dapat meningkatkan kualitas air Waduk Diponegoro Semarang dari kelas D kategori tercemar berat menjadi kelas C kategori tercemar sedang berdasarkan parameter biologi, fisika, dan kimia.Improve the water quality of Diponegoro Reservoir with active charcoal filterClean water must have good quality in terms of physics, chemistry, and biology. Diponegoro Reservoir has a strategic role in the management of Semarang City’s water resources. Based on the results of the survey on Diponegoro Reservoir. The purpose of the study is to improve the water quality of Diponegoro Reservoir based on the class category of water quality index of biological, physical, and chemical parameters with active charcoal filter treatment. Activated charcoal is widely used to remove contaminants in water due to its high capacity for adsorption of the compound, which arises from its large surface area and porosity and can be used to reduce the presence of E. coli in water. The water bacteriological test process is carried out using a method of detection of bacteria with 3M Petrifilm E.coli/Coliform. Methods used in the study include sterilization of tools, sampling of water at inlet and outlet points, filtration of water with the active charcoal thickness of 10, 15, and 20 cm, inoculation of water samples, calculation of the number of colonies, measurement and determination of water quality. The results showed that the thicker the active charcoal media used the better the quality of the water produced. Active charcoal filtration treatment can improve the water quality of Diponegoro Reservoir Semarang from class D category heavily polluted to class C moderately polluted category based on biological, physical, and chemical parameters.
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggraini, Ika Anggraini Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Eka, Prastya Muhammad Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Fransenda, Auxensius Rexer Ginting, Ledy Ginting, R Cinta Badia H Hadiyanto Hadi Endrawati Hapzi Ali Herida, Azalia Puspa Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kumudasmoro, Bagas Mukti Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurmila, Ika Oktavia Pardede, Lasria Pratama, Novi Alvita Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Putri, Adde Lolita Octavia Rejeki Siti Ferniah Rida Yuliana, Rida Safitri, Linda Saniha Adini, Saniha Sarjana Parman Setiawan Wicaksono, Setiawan Siregar, Mangasa Tua Pandapotan Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suryaningsih, Vivi Sutaryo TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W Yeti Darmayati