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PRODUKSI BETA-1,3 GLUKAN DARI Agrobacterium DAN AKTIVITAS PENYEMBUHAN LUKA TERBUKA PADA TIKUS PUTIH Kusmiati, Kusmiati; Rachmawati, Fitria; Siregar, Syafrida; Nuswantara, Sukma; Malik, Amarila
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

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Abstract

Production of beta-1,3 glucan from Agrobacterium and its wound healing activity on white rat. The objective of this study was to determine the activity of beta-1,3 glucan product extracted from local Agrobacterium sp Bro 1.2.1, both wild-type and mutant-type, on opened-wound healing process. Beta-1,3 glucan product was extracted by precipitation, and the purification was carried out by column chromatography as KCl gradient fractions. In this study, white Sprague Dawley rats were employed, and have been treated for opened-wound condition. Seven groups were performed in this experiment, i.e. the negative control, the positive control employing povidone iodine, the two groups of two commercial beta-1,3 glucan with 0,02 mg/4 cm2 each, and the last three groups of beta-1,3 glucan as the test group with 0,02 mg/4 cm2, 0,10 mg/4 cm2 and 0,50 mg/4 cm2, respectively. The result showed significant differences of wound-healing activity performing statistical analysis of the least significance between the negative control, the positive control, as well as the highest dose of the test group of beta-1,3 glucan, at the dose of 0,5 mg/4 cm2 (p<0.05).
PRODUKSI BETA-1,3 GLUKAN DARI Agrobacterium DAN AKTIVITAS PENYEMBUHAN LUKA TERBUKA PADA TIKUS PUTIH Kusmiati, Kusmiati; Rachmawati, Fitria; Siregar, Syafrida; Nuswantara, Sukma; Malik, Amarila
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Production of beta-1,3 glucan from Agrobacterium and its wound healing activity on white rat. The objective of this study was to determine the activity of beta-1,3 glucan product extracted from local Agrobacterium sp Bro 1.2.1, both wild-type and mutant-type, on opened-wound healing process. Beta-1,3 glucan product was extracted by precipitation, and the purification was carried out by column chromatography as KCl gradient fractions. In this study, white Sprague Dawley rats were employed, and have been treated for opened-wound condition. Seven groups were performed in this experiment, i.e. the negative control, the positive control employing povidone iodine, the two groups of two commercial beta-1,3 glucan with 0,02 mg/4 cm2 each, and the last three groups of beta-1,3 glucan as the test group with 0,02 mg/4 cm2, 0,10 mg/4 cm2 and 0,50 mg/4 cm2, respectively. The result showed significant differences of wound-healing activity performing statistical analysis of the least significance between the negative control, the positive control, as well as the highest dose of the test group of beta-1,3 glucan, at the dose of 0,5 mg/4 cm2 (p<0.05).
PENGARUH PENAMBAHAN URASIL DALAM MEDIA FERMENTASI TERHADAP HASIL β-GLUKAN DARI DUA GALUR AGROBACTERIUM Kusmiati, Kusmiati; Tamat, Swasono R.; Nuswantara, Sukma; Muhamad, Salmah
Makara Journal of Science Vol. 11, No. 2
Publisher : UI Scholars Hub

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Abstract

Influence of Uracil in Fermentation Media on β-Glucan Production by Agrobacterium Radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1. Optimum β-glucan production can be achieved by an optimum condition in the fermentation media. Uracil, as a precursor of UDP-glucose, may act as a glucose donor in the formation of polysaccharides such as β-glucan. It is expected that addition of certain quantity of uracil into the fermentation media in a suitable growth phase of Agrobacterium radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1, will significantly increase the β-glucan production. In this investigation, 0.025%; 0.05% or 0.1% of uracil were added into the fermentation media during the logarithmic phase (24 hour) or stationary phase (46 hour) of growth. The β-glucan product was evaluated from the β-glucan (crude) dry-weight and from the β-glucan content. Beta-glucan content was determined as glucose by the Hisamatsu-AOAC and HPLC methods. The highest β-glucan (crude) dry-weight produced by the A. 1.5 was in a medium containg 0.025% uracil (24 hour), whilst by the A. Bro 1.2.1 was in a medium containg 0.1% uracil (46 hour), both higher than control. The highest β-glucan content produced by the A. 1.5 (27.03%) was in a medium containg 0.025% uracil (46 hour), while control produced only 23.28%. The highest β-glucan content produced by the Bro 1.2.1 (29.34%) was in a medium containg 0.025% uracil (24 hour), while control produced only 28.75%. Two-way anova analysis showed that there were no significant influence difference (α = 0,05) from various concentration of uracil in either growth phases, to the yield of β-glucan (crude) dry-weight nor to the β-glucan equivalent glucose content.
PENGEMBANGKAN WISER BERBANTUAN MEDIA REKAM TANGKAP LAYAR UNTUK MENINGKATKAN KETERAMPILAN KOMUNIKASI Kusmiati, Kusmiati
Jurnal Cerdik: Jurnal Pendidikan dan Pengajaran Vol. 2 No. 1 (2022): Jurnal Cerdik: Jurnal Pendidikan dan Pengajaran
Publisher : Jurusan Pendidikan Bahasa, Fakultas Ilmu Budaya, Universita Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jcerdik.2022.002.01.01

Abstract

This article aims to describe the importance of improving students' communication skills by developing WISER assisted with screen capture media on Smart Digital Learning (SDL). Communication skill is a skill that needed in today's era of globalization. Good communication skills can lead students to reach their future. However, in reality, there are still many obstacles to this skill. So, a strategy, as well as media, is needed to support its improvement. This study uses classroom action research using two cycles. The research subjects were 29 students of VII graders. The results showed that the students' communication skills in the first cycle with the percentage of completeness of 89.66% increased to 96.55% in the second cycle. Then, the score for students' communication skills from the category good improved to the very good. The conclusion is that the students' communication skills improve by developing WISER (Wondering habits, Investigating skills, Synthezing skills, Expressing skills , and Reflecting habits) assisted by screen capture media of procedure text in learning to present procedure texts for VII grade of SMP Islam Sabilillah Malang.
ANALISIS NOVEL NIKA BARONTA KARYA ALAN MALINGI: SEBUAH KAJIAN FEMINISME kusmiati, Kusmiati; Darmurtika, Linda Ayu; Suyasa, I Made; Waluyan, Roby Mandalika
Jurnal Ilmiah Telaah Vol 9, No 1: January 2024
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/telaah.v9i1.21559

Abstract

Abstrak: Eksistensi perempuan dalam novel Nika Baronta karya Alan Malingi tidak dapat dipungkiri. Hal ini disebabkan oleh penulis mengungkap fakta sejarah bahwa Jepang pernah menguasai tanah Bima. Jepang menginginkan gadis-gadis Bima sebagai Jugun lanfu. Usaha masyarakat Bima melawan Jepang agar gadis Bima tidak dijadikan pelayan Bar oleh tentara Jepang. Dengan memahami fakta dapat diketahui keberadaan perempuan pada masa penjajahan Jepang. Berdasarkan hal itu maka rumusan masalah yang diangkat dalam penelitian ini adalah Bagaimanakah eksistensi perempuan dalam novel Nika Baronta karya Alan Malingi dalam Perspektif Feminisme. Tujuan penelitian yakni mengkaji tentang eksistensi perempuan dari perspektif feminisme dan selanjutnya mendeskripsikan dan menjawab masalah penelitian secara lebih detail. Adapun manfaat penelitian sebagai berikut Manfaat teoretis dan Manfaat praktis. Metode yang digunakan dalam penelitian ini adalah metode deskriptif kualitatif yang berkaitan dengan teori feminisme. Sumber data dalam penelitian ini adalah novel Nika Baronta karya Alan Malingi. Adapun langkah-langkah untuk menganalisis data yakni 1) Identifikasi, 2) Klasifikasi, 3) Interpretasi. Dari hasil analisis yang telah dilakukan maka kesimpulan yang terdapat dalam novel Nika Baronta karya Alan Malingi yaitu 1) penindasan nilai-nilai budaya, 2) relasi kuasa, 3) budaya patriarki.  Abstract:  The existence of women in the novel Nika Baronta by Alan Malingi cannot be denied. This is because the author reveals the historical fact that Japan once controlled the land of Bima. Japan wanted Bima girls as “Jugun lanfu”. The efforts of the Bima people against the Japanese so that the Bima girls would not be used as barmaids by the Japanese soldiers. By understanding the facts, we can find out the existence of women during the Japanese colonial period. Based on this, the formulation of the problem raised in this research is What is the existence of women in the novel Nika Baronta by Alan Malingi from a Feminist Perspective. The aim of the research is to examine the existence of women from a feminist perspective and then describe the answer to the research problem in more detail. The benefits of research are as follows: Theoretical benefits and practical benefits. The method used in this research is a qualitative descriptive method related to feminist theory. The data source in this research is the novel Nika Baronta by Alan Malingi. The steps for analyzing data are 1) Identification, 2) Classification, 3) Interpretation. From the results of the analysis that has been carried out, the conclusions contained in the novel Nika Baronta by Alan Malingi are 1) oppression of cultural values, 2) socio-economic oppression, 3) power relations, 4) patriarchal culture.                       
PENGARUH KEMAMPUAN KERJA DAN PROFESIONALISME PEGAWAI TERHADAP KUALITAS PELAYANAN KEPADA ANGGOTA DI KOPERASI SATYA ARDHIA BANDARA HALIM PERDANAKUSUMA JAKARTA TIMUR Kusmiati, Kusmiati; Hendra Permadi
Musytari : Neraca Manajemen, Akuntansi, dan Ekonomi Vol. 8 No. 3 (2024): Musytari : Neraca Manajemen, Akuntansi, dan Ekonomi
Publisher : Cahaya Ilmu Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.8734/musytari.v8i3.5582

Abstract

Kualitas Pelayanan adalah fasilitas yang disediakan oleh institusi guna memudahkan pelanggan untuk memanfaatkan layanan yang diberikan oleh pegawai secara langsung. Pada penelitian ini menggunakan pendekatan kuantitatif dengan tujuan untuk mengetahui pengaruh Kemampuan Kerja (X1) dan Profesionalisme Pegawai (X2) terhadap Kualitas Pelayanan (Y). Pengumpulan data dilakukan dengan cara menyebar kuesioner kepada 62 anggota Koperasi Satya Ardhia di Bandara Halim Perdanakusuma Jakarta Timur. Hasil penelitian ini menunjukkan bahwa Kemampuan kerja dan Profesionalisme Pegawai berpengaruh positif dan tidak signifikan terhadap Kualitas Pelayanan kepada anggota di Koperasi Satya Ardhia Bandara Halim Perdanakusuma Jakarta Timur secara parsial maupun secara simultan. Besaran pengaruh sebesar 20,4% variabel Kemampuan Kerja dan Profesionalisme Pegawai dapat mempengaruhi Kualitas Pelayanan kepada anggota di Koperasi Satya Ardhia Bandara Halim Perdanakusuma Jakarta Timur, sedangkan sisanya yaitu sebesar 79,6% lainnya dipengaruhi oleh variabel lain diluar penelitian ini.
Tingkat Ketergantungan Pasien dan Hubungannya dengan Beban Kerja Perawat Kusmiati, Kusmiati; Kamilah, Siti; Lannasari, Lannasari
Journal of Management Nursing Vol. 3 No. 1 (2023): Journal of Management Nursing
Publisher : Scipro Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53801/jmn.v3i1.159

Abstract

Latar Belakang: Tingkat ketergantungan pasien  saat melakukan aktivitas dasar sehari-hari (Activity Daily Living/ADL) berbeda-beda, dan dapat mempengaruhi beban kerja perawat. Untuk mengukur tingkat ketergantungan pasien dalam pemenuhan kebutuhan sehari-hari dapat dilakukan dengan indeks Barthel. Tingkat ketergantungan akan mempengaruhi waktu pelayanan yang diberikan perawat kepada pasien. Beban kerja akan meningkat apabila waktu yang diperlukan perawat saat mendampingi pasien melakukan pemenuhan kebutuhan sehari-hari melebihi dari rata-rata waktu perawatan langsung yang diperlukan perawat ke pasien yaitu 4 jam/pasien/hari. Tujuan: Untuk mengetahui hubungan tingkat ketergantungan pasien dengan beban kerja perawat di ruang rawat inap rumah sakit BSH. Metode: Desain penelitian menggunakan jenis kuantitatif dengan pendekatan cross-sectional. Populasi dalam penelitian ini adalah seluruh perawat rawat inap di rumah sakit BSH dengan sampel berjumlah 31 perawat yang diambil dengan tehnik total sampling. Pengumpulan data menggunakan lembar observasi dan dianalisa menggunakan Uji korelasi Chi-Square. Hasil: Berdasarkan hasil penelitian diketahui bahwa hasil uji statistic  p- Value = 0,019. Kesimpulan: Ada hubungan tingkat ketergantungan pasien dengan beban kerja perawat.
Analysis of Learning Interest, Level of Discipline, and Student’s Responsibility for Post Covid-19 Learning Outcomes Kusmiati, Kusmiati; Ahyani, Nur; Fitria, Happy
Journal of Social Work and Science Education Vol. 5 No. 2 (2024): Journal of Social Work and Science Education
Publisher : Yayasan Sembilan Pemuda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52690/jswse.v5i2.803

Abstract

The COVID-19 pandemic significantly impacted various aspects of life, including education, lifestyle, habits, and social behavior. Online learning at SMA Negeri 1 Jebus faced unstable conditions and limited signal, leading to low discipline and responsibility among students. This decreased interest in learning and quality of education was analyzed in 2021/2022 to understand the impact on post-Covid-19 learning outcomes. This problem made the author analyze students’ interest in learning, level of discipline, and responsibility for post-COVID-19 learning outcomes at SMA Negeri 1 Jebus, West Bangka Regency in 2021/2022. This research uses quantitative methods with a correlational type of research because this research aims to see the relationship between one variable and another variable. In this study, the population was all students at SMA Negeri 1 Jebus, totaling 715 students from class X to class XII. This sample will be taken from class XI students. In this research, the data collection techniques that researchers used were questionnaires and documentation. Based on this research, the results were obtained The study found that interest in learning, discipline, and responsibility significantly influenced post-Covid-19 learning outcomes at SMAN 1 Jebus, West Bangka Regency. The variables of interest in learning, discipline, and responsibility had a positive impact, with the remaining variables being 0.288 or 28.8%. The adjusted R square figure is 0.712 or 71.2%.
EFEKTIVITAS PENERAPAN PERATURAN FULL DAY SCHOOL BAGI PESERTA DIDIK TINGKAT MI DI MI PLUS JÁ-ALHAQ Hidayaturrahman, M.; Fadlillah, Andini; Diah Parmitha, Rike; Kusmiati, Kusmiati; Wafiq Azizah, Anisa
Jurnal Ilmu Pendidikan Islam Vol 23 No No.1 (2025): JIPI: Jurnal Ilmu Pendidikan Islam, Maret 2025
Publisher : FAKULTAS TARBIYAH UNIVERSITAS QOMARUDDIN GRESIK - JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36835/jipi.v23iNo.1.4453

Abstract

This research aims to analyze the effectiveness of implementing Full Day School regulations for Madrasah Ibtidaiyah (MI) level students at MI PLUS JA-ALHAQ Bengkulu City. discipline and student learning achievement, but also raises challenges such as physical and mental fatigue. In conclusion, the implementation of Full Day School is effective in improving the method used in this research is a qualitative descriptive method with data collection techniques through observation, interviews and documentation. The research results show that the implementation of Full Day School has a positive impact on improving the quality of education if it is balanced with fun learning strategies and support from various parties, including teachers, parents and the school environment..
Strategi Pembelajaran Olah Vokal pada Kegiatan Ekstrakurikuler Paduan Suara SMP Negeri 2 Legok Kusmiati, Kusmiati; Lestari, Dwi Junianti; Rizal, Syamsul
Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i2.29372

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui strategi pembelajaran olah vokal yang digunakan dalam kegiatan ekstrakurikuler paduan suara di SMP Negeri 2 Legok, serta dampak penerapan berbagai strategi pembelajaran terhadap perkembangan siswa. Penelitian ini menggabungkan pendekatan deskriptif dengan pendekatan kualitatif. Metode pengumpulan data yang digunakan adalah dokumentasi, wawancara, dan observasi. Temuan penelitian menunjukkan bahwa, dalam hubungannya dengan pendekatan humanisme Carl Rogers dan konstruktivisme Jean Piaget, strategi pembelajaran berikut ini digunakan: pembelajaran langsung, pembelajaran tidak langsung, pembelajaran interaktif, pembelajaran berdasarkan pengalaman, dan pembelajaran mandiri. Telah terbukti bahwa metode ini dapat meningkatkan ekspresi diri, motivasi, keingintahuan, dan bakat menyuarakan pendapat.