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Sunscreen Test of Methanol Extract, N-Hexane, Ethyl Acetate and Methanol-Water Fraction of Artocarpus lanceifolius Roxb. Leaves Based on In Vitro Method Eva Marliana; Novia Rahmawati Isyahro; Nanang Tri Widodo
Jurnal Ilmiah Sains Volume 23 Nomor 2, Oktober 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v23i2.51543

Abstract

Sunscreen test of  keledang (Artocarpus lanceifolius Roxb.) leaves based on in vitro method was carried out. This research aimed to investigate the property of sunscreen from methanol extract, n-hexane, ethyl acetate and methanol-water fraction of keledang leaves. The methods used include the in vitro methods by using spectrophotometer UV-Vis. Based on results of phytochemical test, it was known that  methanol extract contains flavonoids, phenolics, steroids/triterpenoids. n-Hexane fraction contains steroids/triterpenoids and quinones. Ethyl acetate fraction contains flavonoids, phenolics, steroids/triterpenoids and quinones. Methanol-water fraction contains flavonoids and phenolics. Methanol extract, n-hexane, ethyl acetate and methanol-water fraction also showed sunscreen activity based on the SPF value at a concentration of 25; 50; 100; 150; 200 and 250 mg/L. Crude methanol extract has SPF value respectively were 4.44; 7.36; 15.15; 22.47; 31.25 and 37.31 (minimum-ultra). N-Hexane fraction has SPF value respectively were 1.37; 2.24; 5.07; 7.51; 10.01 and 12.74 (minimum-maximum). Ethyl acetate fraction has SPF value respectively were 3.321; 7.20; 13.03; 19.54; 25.97 and 30.59 (minimum-ultra). Methanol-water fraction has SPF value respectively were 5.24; 10.87; 21.48; 30.89; 36.01 and 38.52 (medium-ultra). The SPF value increases with increasing concentration because the higher the concentration, the more active sunscreen compounds contained in the extract and fraction. In keledang leaves, methanol-water fraction has the highest suncreen activity folowed by methanol extract, ethyl acetate fraction and n-hexane fraction. Keledang leaves have a potency as  sunscreen agent. Keywords: Artocarpus lanceifolius; in vitro; sunscreen
POTENTIAL ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF SINGKIL LEAF (Premna cordifolia Roxb.) USE DPPH METHOD: A MINI RIVIEW Firmansyah, Muchammad Ravi; Daniel, Daniel; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Singkil leaves (Premna cordifolia Roxb.) are a plant that is widely found in Kalimantan, and usually grows in the yard of houses or plantations. The antioxidant activity test of methanol extract from Singkil leaves (Premna cordifolia Roxb.) has been done. This research aims to determine the antioxidant properties of methanol extract from leaves. Based on the results of the phytochemical test, it is known that the methanol extract contains alkaloids, flavonoids, phenolics, steroids and triterpenoids. The results of the literature show that the methanol extract exhibits antioxidant activity in the "Strong" category, which has an IC50 value of 77.46 mg/L. Methanol extract of singkil leaves (Premna cordifolia Roxb.) has potential as an antioxidant. Keywords : Singkil leaves (Premna cordifolia Roxb.), antioxidant, DPPH
TEST OF AMYLASE INHIBITION ACTIVITY OF METHANOL EXTRACT SINGKIL LEAF (Premna cordifolia Roxb.) Anggriani, Yusnia Wati; Astuti, Winni; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Singkil (Premna cordifolia Roxb.) is a plant that is widely found un Kalimantan. The people of Kalimantan are often used singkil leaf by as traditional medicine are believed to have medicinal properties as a cure for intestinal worms, colds, and antidiabetic. In this research, it used phytochemical screening and inhibition activity amylase test on singkil’s methanol extract. The objective of this study was to determine in-vitro enzyme amilase inhibition activity against sampel by DNS method. The result of this research was phytochemical screening in singkil’s methanol extract contains alkaloids, flavonoids, saponins and phenolics. Singkil leaf methanol extract at a concentration of 10 µg/mL can inhibit the activity of the amylase enzyme with an inhibition value of 60,93 %. Acarbose used as a positive control in this study showed activity against the amylase of 44,79 %. Amylase inhibition activity of methanol extract was higher than acarbose, the singkil leaf methanol extract has the potential as an antidiabetic drug. Keywords: Premna cordifolia Roxb., Amylase inhibition, Secondary Metabolites.
OPTIMATION TIME PRODUCTION SECONDARY METABOLITES ENDOFIT BACTERIES EXTRACT OF PUCUK MERAH LEAVES (Syzygium myrtifolium Walp.) Rahimah, Priliza; Astuti, Winni; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Pucuk Merah (Syzygium myrtifolium Walp.) is an ornamental plant that has benefits as an antioxidant, antibacterial, antiplatelet, antiulcerative, antimalarial, antiviral, antihyperglycemic and anti-inflammatory. This research aims to determine the optimum time for the production of secondary metabolites of pucuk merah endophytic bacteria for amylase and protease inhibition. Production of secondary metabolites was carried out at various times of 24 hours, 48 ​​hours and 72 hours. The method for the amylase inhibition test used the DNS method and the protease inhibition test used the Bradford method. The results showed that the optimum time for the production of secondary metabolites was 48 hours. Keywords: Syzygium myrtifolium Walp., Endophyte Bacteria, Production Time.
THE POTENTIAL OF EXTRACT SINGKIL LEAVES (Premna cordifolia Roxb.) AS ANTIBACTERIAL AGENTS AGAINST Propionibacterium acnes Az-zahra, Deannisa Khoiru; Astuti, Winni; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

One of the causes of acne is infection by the bacteria Propionibacterium acnes. Singkil (Premna cordifolia Roxb.) is a kind of herb plant from Kutai Kartanegara which is used as a traditional herb including for acne’s treatment. This study aims to know the content of secondary metabolites compound in singkil’s extract and determine the antibacterial activity against Propionibacterium acnes KCCM 41747 bacteria. In this study, it’s phytochemical screening and antibacterial activity test using the disc diffusion method on methanol extract, n-hexane, ethyl acetate and water-methanol fractions. the result of phytochemical screening revealed that methanol extract of singkil contains alkaloids, flavonoids, steroids, and saponins. The n-hexane fraction contains steroids and the ethyl acetate fraction contains alkaloids, flavonoids, and phenolics while the water-methanol fraction contains alkaloids, flavonoids, and phenolics. The antibacterial activity test extract with a concentration of 10% showed that the diameter of clear zone of the metanol extract, n-hexane fraction, ethyl acetate fraction, and water-methanol fraction were 13 mm, 9 mm, 13,67 mm and 6 mm, respectively. The ethyl acetate fraction had the highest antibacterial activity among the four extracts. A minimum inhibitory concentration value of the ethyl acetate fraction against Propionibacterium acnes KCCM 41747 was 0,625%. The ethyl acetate fraction have the potential as an antibacterial against the bacteria Propionibacterium acnes KCCM 41747. Keywords: Premna cordifolia Roxb., Secondary metabolites, Propionibacterium acnes KCCM 41747
UJI AKTIVITAS ANTIBAKTERI RIMPANG KUNYIT PUTIH (Kaempferia rotundaL.) TERHADAP Streptococcus sobrinus DAN Salmonella typhi -, Nurfadzillah; Marliana, Eva; Ruga, Ritbey
JURNAL ATOMIK Vol 6 No 2 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

An infectious disease caused by bacteria is a disease that often occurs in tropical areas. The used of plant extract is an interesting object of study to overcome these infectious diseases and because of an increase in bacterial resistance to antibiotics. White turmeric rhizome widely used by Indonesia society as an herb which has been used for the treatment of diarrhea and abdominal pain. This study aims to determine the content of secondary metabolities and inhibitory of white turmeric rhizome (Kaempferia rotunda L.) against Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 bacteria. The results of phytochemical screening white turmeric rhizome contained compound triterpenoids, steroids, phenolics, and saponins. An antibacterial activity test used agar diffusion method against Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 bacteria. Antibacterial activity white turmeric rhizome (Kaempferia rotunda L.) with concentration 1, 2, 4, 8% have an inhibition zone diameter against Streptococcus sobrinus KCCM 11898 bacteria is 10.33, 10.67, 17 and 25.17 mm respectively with the antibacterial strength is strong, meanwhile in Salmonella typhi ATCC 422 bacteria have an inhibition zone diameter was 8, 8.3, 11.83 and 13 mm respectively with the antibacterial strength is intermediate. Keywords: Antibacterial, White Turmeric Rhizome (Kaempferia rotunda L.), Agar Difussion
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL KULIT BUAH PISANG AMBON (Musa paradisiaca var. sapientum (L.) Kunt.) DENGAN METODE DPPH Tullah, Mirna Hidaya; Marliana, Eva; Erwin, Erwin
JURNAL ATOMIK Vol 8 No 2 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Test of antioxidant activity of ambon banana peel (Musa paradisiaca var. sapientum (L.) Kunt.) methanol extract has been done. Test were carried out using a UV-Vis spectrophotometer based on the DPPH radical scavenging method with positive control of vitamin C. Antioxidant activity was obtained based on the IC50 value of 104,31 mg/L. IC50 value indicates that the peel of Ambon banana (Musa paradisiaca var. sapientum (L.) Kunt.) has antioxidant activity which is included in the moderate category.
POTENSI ANTIBAKTERI EKSTRAK ETANOL MENIRAN (Phyllanthus niruri L.) TERHADAP BAKTERI Streptococcus sobrinus DAN Salmonella typhi Rakasiwi, Dea; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 8 No 1 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Meniran (Phyllanthus niruri L.) is one of the herb plants used as traditional medicine, such as toothache medicine and diarrhea medicine. The purposed of this study was to determined the type of secondary metabolites in the ethanol extract of meniran herb and to determined the minimum inhibitory concentration (MIC) value of the ethanol extract of meniran (Phyllanthus niruri L.) to Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422. The ethanol extract of meniran was tested for phytochemicals. The MIC value of the ethanol extract of meniran herb for the test bacteria was obtained by varying the concentration of the ethanol extract of meniran herbs. Phytochemical test results showed that the ethanol extract of meniran herbs contains alkaloids, phenolics and steroids. The minimum inhibitory concentration value of the ethanol extract of meniran herb at the bacteria Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 each was 0.625%. Keywords: Phyllanthus niruri L, minimum inhibitory concentration (MIC), Streptococcus sobrinus KCCM 11898, Salmonella typhi ATCC 422
PHYTOCHEMICAL TEST AND TOXICITY (Brine Shrimp Lethality Test) IN ETHANOL EXTRACT, ETHYL ACETATE FRACTION AND RESIDUAL ETHANOL FRACTION OF AJERAN LEAVES (Bidens pilosa L.) Audia, Nuraini Meti; -, Daniel; Marliana, Eva
JURNAL ATOMIK Vol 5 No 2 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test and toxicity in ethanol extract, ethyl acetate fraction and residual ethanol fraction of ajeran leaves (Bidens pilosa L.) has been done. The method used to determine secondary metabolites is clualitative test and the value of Lethal Concentration (LC50) with the Brine Shrimp Lethality Test (BSLT). Phytochemical test results showed that the ethanol extract and ethyl acetate fraction contained flavonoid, steroid and phenol compound. Residual ethanol fraction contained flavonoid and phenol compound. Toxicity test showed ethanol extract had LC50 value of 862.19 ppm. Ethyl acetate fraction has LC50 value of 8.26 ppm. Residual ethanol fraction had LC50 value of 275.24 ppm. The results of the toxicity test can be concluded that the ethyl acetate fraction very toxic category, ethanol extract and residual ethanol fraction included toxic category. The results that obtained from GC-MS characterization were steroid
TEST OF AMYLASE INHIBITION ACTIVITY OF Melicope PLANTS WHICH POTENTIAL AS ANTIDIABETIC AGENTS Nurjanah, Siti; Marliana, Eva; Astuti, Winni
JURNAL ATOMIK Vol 5 No 2 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The test of amylase inhibition activity of the leaves of Melicope lunu-ankenda and bark of Melicope glabra (BI.) T.G. Hartley extracts were done. The objective of this study was to determine in-vitro α-amylase inhibition activity against two samples by DNS method. The result showed that two samples of Melicope lunu-ankenda leaves and bark of Melicope glabra (BI.) TG Hartley displayed the enzyme inhibitory effect with percent inhibitions of 48.71% and 46.36%. Two sample extracts can inhibit amylase. It can be suggested that about sample were potential as antidiabetic agents.
Co-Authors -, Nurfadzillah Afandi, Irvan Agustina R. M. Alma Yulvita Pebriana Amalia, Elfiza Aman S. Panggabean Anggriani, Yusnia Wati Aryoga Oktabriangga Saputra Audia, Nuraini Meti Ayu Mellyanti Az-zahra, Deannisa Khoiru Chairul salaeh Chairul Saleh Daniel Daniel -, Daniel Daniel Daniel Daniel Daniel Daniel Daniel Daniel Daniel Dewi, Dynda Rosiana Djihan Ryn Pratiwi Djihan Ryn Pratiwi, Djihan Ryn Doni Prio Atmoko Dwi Lestari Ersa Marina Rahmawati Erwin Erwin Erwin Erwin Erwin Erwin Erwin Erwin Fikriah, Ika Firmansyah, Muchammad Ravi Hairani, Rita Husnul Chatimah Ika Yekti Liana Sari Indra Masmur irfa Ashari Hyahara Isyahro, Novia Rahmawati Jumaidil Awal K Y Margiati, K Y Kim, Soo-Ya Kim, Yong-ung Kusuma, Irawan W. Madaniah Fathirah Magdaleni, Agustina Rahayu Masmur, Hestina Masruhin, Muhammad A. Medi Hendra Medi Hendra, Medi Mikdariawan, Adha Muh. Naufal Hisyam Muhammad Marion Akbar Mulyadi Tanjung Mulyana Mulyana Nabila, Sahira Fara Nanang Tri Widodo Nanang Tri Widodo Nanang Tri Widodo Nisa, Nahdia Khairun Noor Hindryawati Noor Hindryawati, Noor Novia Rahmawati Isyahro Novia Rahmawati Isyahro Nur Aina Nur Hidayah Nur Khasanah Nur Khsanah Nurul Jannah Pramana, Aditiya Hadi Ryan Purnamasari, Ade Rezki Rachman, Ferry Aditya Rahimah, Priliza Rahmat Gunawan Rakasiwi, Dea Ramadhani, Riski Vebryanti Ratna Kusuma Rismawati Rismawati Rita Hairani Ritbey Ruga Ritson Purba, Ritson Rudi Kartika, Rudi Ruga, Ritbey Saibun Sitorus Siti Halidjah Siti Nurjanah Soerja Koesnarpadi Subur P Pasaribu Swandari Paramita Syamsul Arifin Teguh Wirawan, Teguh Tjitjik Sri Tjahjandarie Tjitjik Srie Tjahjandarie Tullah, Mirna Hidaya Winni Astuti Winni Astuti, Winni