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SKRINING BAKTERI DARI AIR SUNGAI KARANG MUMUS SEBAGAI PENGHASIL ENZIM PROTEASE, SELULASE DAN LIPASE Amantha, Dhita Novalia; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1402

Abstract

Bacteria from polluted waters have the potential to produce extracellular hydrolase enzymes, namely protease enzymes, cellulase and lipase. This study was conducted to isolate bacteria from the water of the Karang Mumus River around Segiri Market, Samarinda as a producer of hydrolase enzymes. The bacteria were isolated in a nutrient-dense agar (NA) medium by the streak method. The results of bacterial isolation were obtained from 14 single colonies of bacteria. Single colonies of bacteria are selected to produce hydrolase enzymes in the form of proteases, cellulases and lipases. The results of the selection of hydrolase enzyme-producing bacteria were 3 protease-producing isolates, 10 cellulase-producing isolates, and 5 lipase-producing isolates.
ADSORPSI METHYLENE BLUE OLEH ARANG AKTIF BIJI BUAH LAI (DURIO KUTEJENSIS) -, Mulyana; Wirawan, Teguh; Marliana, Eva
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1437

Abstract

The research on the adsorption of methylene blue dye by activated charcoal of lai fruit seeds has been conducted. The purpose of this study was to determine the optimum pH and time as well as the maximum adsorption capacity and isotherm type (Langmuir and Freundlich) of activated charcoal of lai fruit seeds in adsorbing methylene blue dye. In this study, activated charcoal was made through a carbonation process at a temperature of 500 oC for 4 hours and was chemically activated by soaking in 2 M HCl for 24 hours. Characterization of activated charcoal was carried out using water content test, ash content, Fourier Transform Infrared (FTIR) spectrum analysis, and Scanning Electron Microscopy (SEM). In lai seed charcoal, the water content value was 8.97% and in lai seed activated charcoal it was 8.27%, the ash content was 0.16% in lai seed charcoal and 0.09% in lai seed activated charcoal. The surface area of lai seed charcoal and lai seed activated charcoal obtained values of 72.201 m²/g and 80.230 m²/g respectively. The results of the FTIR spectra on lai seed activated charcoal showed the presence of C-H (1426.21 cm-1), C=C (1591.65 cm-1), C=O (1690.06 cm-1), O-H (3320.22 cm-1). Based on the results of SEM on lai seed charcoal, the particle surface looks rough and uneven, and has pores and a larger surface area. The adsorption of methylene blue follows the Freundlich isotherm model. The methylene blue adsorption test showed an optimum pH at 8, and an optimum time of 45 minutes with a maximum adsorption capacity of 32.69 mg/g.
Co-Authors -, Nurfadzillah Afandi, Irvan Agustina R. M. Alma Yulvita Pebriana Amalia, Elfiza Aman S. Panggabean Amantha, Dhita Novalia Anggriani, Yusnia Wati Aryoga Oktabriangga Saputra Audia, Nuraini Meti Ayu Mellyanti Az-zahra, Deannisa Khoiru Chairul salaeh Chairul Saleh Daniel Daniel -, Daniel Daniel Daniel Daniel Daniel Daniel Daniel Daniel Daniel Dewi, Dynda Rosiana Djihan Ryn Pratiwi Djihan Ryn Pratiwi, Djihan Ryn Doni Prio Atmoko Dwi Lestari Ersa Marina Rahmawati Erwin Erwin Erwin Erwin Erwin Erwin Erwin Erwin Fikriah, Ika Firmansyah, Muchammad Ravi Hairani, Rita Husnul Chatimah Ika Yekti Liana Sari Indra Masmur irfa Ashari Hyahara Isyahro, Novia Rahmawati Jumaidil Awal K Y Margiati, K Y Kim, Soo-Ya Kim, Yong-ung Kusuma, Irawan W. Madaniah Fathirah Magdaleni, Agustina Rahayu Masmur, Hestina Masruhin, Muhammad A. Medi Hendra Medi Hendra, Medi Mikdariawan, Adha Muh. Naufal Hisyam Muhammad Marion Akbar Mulyadi Tanjung Mulyana Mulyana Mulyana Mulyana Nabila, Sahira Fara Nanang Tri Widodo Nanang Tri Widodo Nanang Tri Widodo Nisa, Nahdia Khairun Noor Hindryawati Noor Hindryawati, Noor Novia Rahmawati Isyahro Novia Rahmawati Isyahro Nur Aina Nur Hidayah Nur Khasanah Nur Khsanah Nurul Jannah Pramana, Aditiya Hadi Ryan Purnamasari, Ade Rezki Rachman, Ferry Aditya Rahimah, Priliza Rahmat Gunawan Rakasiwi, Dea Ramadhani, Riski Vebryanti Ratna Kusuma Rismawati Rismawati Rita Hairani Ritbey Ruga Ritson Purba, Ritson Rudi Kartika, Rudi Ruga, Ritbey Saibun Sitorus Siti Halidjah Siti Nurjanah Soerja Koesnarpadi Subur P Pasaribu Swandari Paramita Syamsul Arifin T, Nurnaningsi Teguh Wirawan, Teguh Tjitjik Sri Tjahjandarie Tjitjik Srie Tjahjandarie Tullah, Mirna Hidaya Winni Astuti Winni Astuti, Winni