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UJI FITOKIMIA DAN ANTIBAKTERI EKSTRAK METANOL DAUN MACARANGA LAMELLATA WHITMORE Ramadhani, Riski Vebryanti; Marliana, Eva; Erwin, Erwin
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test and antibacterial activity of Macaranga lamellata Whitmore have been done. This research aims to find out the secondary metabolites using Thin Layer Chromatography (TLC) and to determine antibacterial activity by paper disc method from methanol extract on Macaranga lamellata Whitmore leaf. Based on the results obtained from phytochemical tests, it was known that methanol extract contains alkaloid, flavonoid, polyphenol and terpenoid/steroid compounds. Antibacterial activity obtained Minimum Inhibitory Concentration values from methanol extract for Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 at 0-0.5%.
UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL AKAR MERUNG (Coptosapelta tomentosa) Dewi, Dynda Rosiana; Marliana, Eva; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical tests and antibacterial activity of the roots of Merung plants (Coptosapelta tomentosa) have been done. This research aims to find out the content of secondary metabolites contained in the sample using the Thin Layer Chromatography method and to determine antibacterial activity by paper disc method from methanol extract Merung root (Coptosapelta tomemtosa). Based on results of phytochemical screening, it was known that methanol extract contained alkaloid, polyphenol, flavonoid and terpenoid/steroid. Methanol extract antibacterial activity against Propionibacterium acnes KCCM 11898 range of MIC of 0-1%. Methanol extract showed antibacterial activity against Escherichia coli bacteria ATCC 25922 range of MIC of 0-1%. From the results of the antibacterial activity test it can be concluded that the methanol extract have antibacterial activity against Propionibacterium acne KCCM 11898 and Escherichia coli ATCC 25922.
PHYTOCHEMICAL TEST ON METHANOL EXTRACT OF LEAF OF Macaranga hullettii King ex Hook.f. Rismawati, Rismawati; Marliana, Eva; Daniel, Daniel
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test of Macaranga hullettii King ex Hook.f have been done. This research aims to find out the secondary metabolites using Thin Layer Chromatography (TLC) from methanol extract on Macaranga hullettii leaf. Based on results of phytochemical screening, it is known that methanol extract contains alkaloid, steroid/triterpenoid, polifenol and flavonoid compounds.
Synthesis of Silver Nanoparticle Using Bio-Reductant from Wuluh Star Fruit (Averrhoa Bilimbi L.) Extract and Their Activity as Sunscreen Hindryawati, Noor; Muhammad Marion Akbar; Eva Marliana; Ratna Kusuma
Jurnal Bahan Alam Terbarukan Vol. 14 No. 1 (2025): June 2025 [Nationally Accredited Sinta 3]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v14i1.16552

Abstract

Synthesis of silver nanoparticles has been carried out using AgNO3 solution as precursor with wuluh star fruit extract as bioreductant and reacted under the sunlight. The synthesized silver nanoparticles were tested for its sunscreen activity. The synthesized has been done with optimation of AgNO3 precursor and bioreductant. Based on absorbances value using UV-Vis Spectrophotometer, it is shown that silver nanoparticles from 1,5 mM AgNO3 and 25% (v/v) extract are more stable than the other variation for 4 days. The characterization of silver nanoparticles using Transmission Electron Microscope (TEM) and Particle Size Analyzer (PSA) instrument result shows the morphology of rounded silver nanoparticles with diverse sizes at range 6,9 – 27,7 nm, and with average particle size of 14,8 nm. Particle Size Analyzer (PSA) instrument shows the distribution of silver nanoparticles at range under 100 nm. Sunscreen activity of silver nanoparticles shows the SPF value of 44,14 with ultra protection type and on high level. The SPF value obtained indicates that silver nanoparticles can be applied as sunscreen.  
UJI AKTIVITAS ANTIOKSIDAN KOMBINASI ESKTRAK METANOL DAUN REMEK DAGING (Hemigraphis colorata W.Bull) DAN DAUN KRATOM (Mitragyna speciosa Korth) Aina, Nur; Saleh, Chairul; Marliana, Eva
JURNAL ATOMIK Vol 10 No 1 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i1.1483

Abstract

Antioxidant activity test of a combination of methanol extracts of meat remek leaves (Hemigraphis colorata w. bull) and kratom leaves (Mitragyna speciosa Korth) using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) silencing method with vitamin C as a positive control was analysed by UV-Vis spectrophotometry. The methanol extract of remek leaves contains flavonoids, triterpenoids/steroids and phenolic compound while the methanol extract of kratom leaves contains triterpenoids, flavonoids, alkaloids, saponins and phenolic. The combination of the two extracts gave the highest % inhibition in the 1:1 ratio. The IC50 values of methanol extracts of meat remek leaves, kratom leaves are 70.61; 33.43 ppm indicating antioxidant activity in the strong and very strong categories and the combination is 38.08 indicating the combination index is strong antagonis
The Utilization of Chitosan from Maggot (Hermetia illucens) Exuvia as Edible Coating for Tomatoes (Lycopersicon esculentum) and Edible Film with the Addition of Honey as an Antibacterial Agent Daniel; Sitorus, Saibun; Pasaribu, Subur P.; Marliana, Eva; Hairani, Rita; Ruga, Ritbey; Purba, Ritson; Saleh, Chairul; Pratiwi, Djihan Ryn; Magdaleni, Agustina Rahayu; Masmur, Indra; Masmur, Hestina
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1232-1241

Abstract

The use of chitosan from maggot (Hermetia illucens) exuvia as an edible coating for tomatoes (Lycopersicon esculentum) and an edible film with honey added as an antibacterial agent was investigated. This study aimed to determine the yield of chitosan from black soldier fly (BSF, Hermetia illucens) maggot exuviae as an edible coating. The chitosan was produced from chitin by deacetylation, and its effect on the weight loss and vitamin C levels of tomatoes for 7 days after treatment was investigated. The chitosan coating was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy. The antibacterial activity was evaluated using a disk diffusion method. The result showed that by deacetylation, conversion of chitin produced chitosan with a yield of 72% and a deacetylation degree of 75.05%. The chitosan coating significantly affected the weight loss of tomatoes, with the best concentration being 2%. However, the treatment did not significantly affect the decrease in vitamin C levels. The edible chitosan film from BSF maggot exuviae with added honey had a thickness, water content, and water vapor transmission rate of 0.156 mm, 16.9913%, and 30.45 g/m2/24 hours, respectively. SEM characterization showed a relatively dense surface structure, which was slightly smooth and porous. Regarding antibacterial activity, the edible chitosan film inhibited Staphylococcus aureus with an inhibition zone of 10.37 mm; however, Escherichia coli was not inhibited. These results suggest that chitosan from BSF maggot exuviae has potential as an effective edible coating for reducing weight loss in tomatoes despite showing limited antibacterial properties.
Pharmacological Potential of the Stem of Butterfly Root Plant (Phanera semibifida Roxb. var. semibifida): A Phytochemical, Toxicity, and Anti-Inflammatory Activity Study Ayu Mellyanti; Rita Hairani; Erwin Erwin; Winni Astuti; Medi Hendra; Eva Marliana
Jurnal Ilmiah Sains Volume 25 Issue 2, October 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v25i2.62442

Abstract

Phanera semibifida Roxb. var. semibifida, commonly known as the butterfly root plant, is one of the plants frequently used by various indigenous tribes in Kalimantan as a traditional medicinal plant. This study was conducted to identify the secondary metabolite compounds, toxicity level, and anti-inflammatory activity of the plant. Extraction of the stem of the butterfly root plant was carried out using the maceration method with methanol as the solvent, followed by partitioning with n-hexane and ethyl acetate. Phytochemical screening of the stem was performed using Thin Layer Chromatography (TLC). Toxicity testing was conducted using the Brine Shrimp Lethality Test (BSLT), and anti-inflammatory activity was evaluated based on protein denaturation inhibition in vitro using a ultraviolet-visible (UV-Vis) spectrophotometer. The crude extract of the stem was found to contain alkaloids, triterpenoids, steroids, flavonoids, and phenolics; the n-hexane fraction contained triterpenoids and steroids; the ethyl acetate fraction contained alkaloids, triterpenoids, steroids, flavonoids, and phenolics, while the aqueous fraction contained alkaloids, flavonoids, and phenolics. The toxicity levels (LC₅₀) of the crude extract, n-hexane fraction, ethyl acetate fraction, and aqueous fraction were 57.45; 158.04; 42.34; and 72.88 µg/mL, respectively, indicating toxic activity against Artemia salina L. larvae. The anti-inflammatory activity levels (IC₅₀) of the crude extract, n-hexane fraction, ethyl acetate fraction, and aqueous fraction were 124.95 µg/mL (moderate category); 161.27 µg/mL (moderate category); 99.15 µg/mL (strong category); and 143.44 µg/mL (moderate category), respectively. These results indicate that the stem of the butterfly root plant (Phanera semibifida Roxb. var. semibifida) contains bioactive compounds and has potential as a natural anti-inflammatory agent.
The Potential oOf Butterfly Root Plant (Phanera semibifida Roxb. var. semibifida) Stem as a Source of Antioxidants and Natural Sunscreen Agent T, Nurnaningsi; Hairani, Rita; Saleh, Chairul; Ruga, Ritbey; Marliana, Eva; Hendra, Medi
JURNAL KIMIA MULAWARMAN Vol 23 No 1 (2025)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v23i1.1559

Abstract

Phanera semibifida Roxb. var. semibifida merupakan tanaman liana yang berasal dari famili Leguminosae. Tanaman ini umum digunakan sebagai tanaman obat oleh berbagai suku bangsa di Kalimantan. Penelitian ini bertujuan untuk mengidentifikasi metabolit sekunder menggunakan metode kromatografi lapis tipis (KLT), mengevaluasi aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil), dan menilai potensi tabir surya secara in vitro menggunakan spektrofotometer UV-Vis. Uji fitokimia menunjukkan bahwa baik ekstrak metanol kasar maupun fraksi etil asetat mengandung alkaloid, steroid, triterpenoid, fenolik, dan flavonoid. Fraksi n-heksana mengandung steroid dan triterpenoid. Fraksi air mengandung alkaloid, fenolik, dan flavonoid. Aktivitas antioksidan diamati dalam ekstrak metanol kasar, n-heksana, etil asetat, dan fraksi air dengan nilai IC₅₀ masing-masing 19,51; 105,08; 16,99 dan 137,30 mg/L. Aktivitas tabir surya ditentukan berdasarkan nilai SPF pada konsentrasi 25, 50, 100, 150, 200, dan 250 mg/L untuk ekstrak kasar, fraksi n-heksana, etil asetat, dan air, yang masing-masing berkisar antara 0,52–6,55, 0,63–6,91, 1,51–17,93, dan 0,23–3,01. Hasil ini menunjukkan potensi Phanera semibifida Roxb. var. semibifida sebagai kandidat alami dengan aktivitas antioksidan dan sifat pelindung matahari.
SKRINING BAKTERI DARI AIR SUNGAI KARANG MUMUS SEBAGAI PENGHASIL ENZIM PROTEASE, SELULASE DAN LIPASE Amantha, Dhita Novalia; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1402

Abstract

Bacteria from polluted waters have the potential to produce extracellular hydrolase enzymes, namely protease enzymes, cellulase and lipase. This study was conducted to isolate bacteria from the water of the Karang Mumus River around Segiri Market, Samarinda as a producer of hydrolase enzymes. The bacteria were isolated in a nutrient-dense agar (NA) medium by the streak method. The results of bacterial isolation were obtained from 14 single colonies of bacteria. Single colonies of bacteria are selected to produce hydrolase enzymes in the form of proteases, cellulases and lipases. The results of the selection of hydrolase enzyme-producing bacteria were 3 protease-producing isolates, 10 cellulase-producing isolates, and 5 lipase-producing isolates.
ADSORPSI METHYLENE BLUE OLEH ARANG AKTIF BIJI BUAH LAI (DURIO KUTEJENSIS) -, Mulyana; Wirawan, Teguh; Marliana, Eva
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1437

Abstract

The research on the adsorption of methylene blue dye by activated charcoal of lai fruit seeds has been conducted. The purpose of this study was to determine the optimum pH and time as well as the maximum adsorption capacity and isotherm type (Langmuir and Freundlich) of activated charcoal of lai fruit seeds in adsorbing methylene blue dye. In this study, activated charcoal was made through a carbonation process at a temperature of 500 oC for 4 hours and was chemically activated by soaking in 2 M HCl for 24 hours. Characterization of activated charcoal was carried out using water content test, ash content, Fourier Transform Infrared (FTIR) spectrum analysis, and Scanning Electron Microscopy (SEM). In lai seed charcoal, the water content value was 8.97% and in lai seed activated charcoal it was 8.27%, the ash content was 0.16% in lai seed charcoal and 0.09% in lai seed activated charcoal. The surface area of lai seed charcoal and lai seed activated charcoal obtained values of 72.201 m²/g and 80.230 m²/g respectively. The results of the FTIR spectra on lai seed activated charcoal showed the presence of C-H (1426.21 cm-1), C=C (1591.65 cm-1), C=O (1690.06 cm-1), O-H (3320.22 cm-1). Based on the results of SEM on lai seed charcoal, the particle surface looks rough and uneven, and has pores and a larger surface area. The adsorption of methylene blue follows the Freundlich isotherm model. The methylene blue adsorption test showed an optimum pH at 8, and an optimum time of 45 minutes with a maximum adsorption capacity of 32.69 mg/g.
Co-Authors -, Nurfadzillah Afandi, Irvan Agustina R. M. Alma Yulvita Pebriana Amalia, Elfiza Aman S. Panggabean Amantha, Dhita Novalia Anggriani, Yusnia Wati Aryoga Oktabriangga Saputra Audia, Nuraini Meti Ayu Mellyanti Az-zahra, Deannisa Khoiru Chairul salaeh Daniel Daniel -, Daniel Daniel Daniel Daniel Daniel Daniel Daniel Daniel Daniel Dewi, Dynda Rosiana Djihan Ryn Pratiwi Djihan Ryn Pratiwi, Djihan Ryn Doni Prio Atmoko Dwi Lestari Ersa Marina Rahmawati Erwin Erwin Erwin Erwin Erwin Erwin Erwin Erwin Fikriah, Ika Firmansyah, Muchammad Ravi Husnul Chatimah Ika Yekti Liana Sari Indra Masmur irfa Ashari Hyahara Isyahro, Novia Rahmawati Jumaidil Awal K Y Margiati, K Y Kim, Soo-Ya Kim, Yong-ung Kusuma, Irawan W. Madaniah Fathirah Magdaleni, Agustina Rahayu Masmur, Hestina Masruhin, Muhammad A. Medi Hendra Medi Hendra, Medi Mikdariawan, Adha Muh. Naufal Hisyam Muhammad Marion Akbar Mulyadi Tanjung Mulyana Mulyana Mulyana Mulyana Nabila, Sahira Fara Nanang Tri Widodo Nanang Tri Widodo Nanang Tri Widodo Nisa, Nahdia Khairun Noor Hindryawati Noor Hindryawati, Noor Novia Rahmawati Isyahro Novia Rahmawati Isyahro Nur Aina Nur Hidayah Nur Khasanah Nur Khsanah Nurul Jannah Pramana, Aditiya Hadi Ryan Purnamasari, Ade Rezki Rachman, Ferry Aditya Rahimah, Priliza Rahmat Gunawan Rakasiwi, Dea Ramadhani, Riski Vebryanti Ratih Dewi Saputri Ratna Kusuma Rismawati Rismawati Rita Hairani Ritbey Ruga Ritson Purba, Ritson Rudi Kartika, Rudi Ruga, Ritbey Saibun Sitorus Saleh, Chairul Siti Halidjah Siti Mariyatul Ulfah Siti Nurjanah Soerja Koesnarpadi Subur P Pasaribu Swandari Paramita Syamsul Arifin T, Nurnaningsi Teguh Wirawan, Teguh Tjitjik Sri Tjahjandarie Tjitjik Srie Tjahjandarie Tullah, Mirna Hidaya Winni Astuti Winni Astuti, Winni