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All Journal Medical Journal of Indonesia Universa Medicina Journal of General Procedural Dermatology and Venereology Indonesia Majalah Kedokteran Andalas Media Dermato-Venereologica Indonesiana Journal of General-Procedural Dermatology & Venereology Indonesia Bali Dermatology Venereology and Aesthetic Journal Jurnal Kedokteran Meditek Ophthalmologica Indonesiana
Wresti Indriatmi B. Makes
Departemen Dermatologi Dan Venereologi, Fakultas Kedokteran Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Journal : Universa Medicina

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Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum Effendi, Ida; Rosana, Yeva; Yasmon, Andi; Indriatmi, Wresti
Universa Medicina Vol 37, No 1 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2018.v37.75-84

Abstract

BackgroundSyphilis is a multistage disease transmitted primarily through sexual intercourse. Nowadays, the polymerase chain reaction (PCR) test for Treponema pallidum has been widely used and is expected to overcome problems in diagnostic tests for syphilis. The Treponema pallidum PCR is influenced by type of specimens, PCR methods and target genes. This study aimed to assess the use of blood and serum in multiplex nested PCR for Treponema pallidum, targeting the 23S rRNA.MethodsA cross-sectional study was conducted from April 2015 - April 2016. Sampling was carried out consecutively among patients with clinical features of secondary syphilis who came to Sexually Transmitted Disease (STD) clinics in Jakarta. All sera were also tested with Rapid Plasma Reagin (RPR) and Treponema pallidum Hemagglutination Assay (TPHA) assay, which was considered as the gold standard for this study. We determined the sensitivity and specificity of the multiplex nested PCR for Treponema pallidum using blood and serum.ResultsPCR test was performed on 122 clinical specimens (61 blood and 61 serum). The positive results of PCR test on blood was 22.95% and serum was 6.56%, while the positive results of serology was 68.85%. The sensitivity of Treponema pallidum multiplex nested PCR on blood was 30.95% compared to serum 9.52% (p=0.006). PCR test on blood is able to detect 3.25 times higher than serum. ConclusionThe use of blood has a higher proportion of positives compared to serum in Treponema pallidum multiplex nested PCR using 23S rRNA target gene.
Microscopic examination using negative staining for rapid diagnosis of syphilis Rosana, Yeva; Effendi, Ida; Indriatmi, Wresti; Yasmon, Andi
Universa Medicina Vol. 41 No. 1 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.64-70

Abstract

BACKGROUNDSyphilis is a global health problem, especially in developing countries including Indonesia. Treponema pallidum, the etiologic agent of syphilis, cannot be cultured in vitro. Syphilis has several clinical manifestations, making laboratory testing a very important aspect of diagnosis. Microscopic examination may support the diagnosis but is rarely used in Indonesia. The aim of this study was to evaluate negative staining using the light microscope to detect T. pallidum in syphilitic lesions. METHODSA cross-sectional study was conducted involving 27 subjects who came to several dermato-venereology clinics in Jakarta. Exudates were collected from genital ulcers, condylomata lata, and dry mucocutaneous rash on palms and soles of syphilis patients. Negative staining using one drop of Indian ink was used to examine for treponemas under the light microscope at 10x100 magnification. RESULTSMicroscopic examination using negative staining showed a few clusters of small and spiral shaped bacteria. Of the 39 specimens from 27 subjects, microscopic examinations were successfully done on 10 specimens. Observations could only be conducted on 5 specimens, 3 (60.0%) of which showed the morphology of spirochetes. This examination is the easiest method for detecting the bacteria. Moreover, the bacteria that were isolated from painless genital ulcers could be observed more clearly than those from erythematous maculopapular lesions. CONCLUSIONTreponema pallidum was successfully detected by microscopic examination in all moist lesions, but was difficult to detect in dry lesions. Negative staining under the light microscope appears to be simple, affordable, and available in most microbiology laboratories in Indonesia.
Co-Authors Adisasmito, Ayutika Saraswati Ahmad Yudianto Aldri Frinaldi Andi Yasmon Anggraini, Ika Anissa, Lidwina Aria Kekalih Arlha Aporia Debinta Benny Nelson Budi Iman Santoso Caroline Oktarina Christa Desire Gracia Djaja Surya Atmadja Efendi, Ida Effendi, Ida Emma Rusmayani Endi Novianto Erwinanto Evita Halim Effendi Evita Halim Effendi Fathan, Hafiza Fauziah, Siti Nurani Fourina, Peppy Githa Rahmayunita Gultom, Desy A. Hafiza Fathan Halim, Melissa Hanny Nilasari Harrina Erlianti Rahardjo, Harrina Erlianti Hartono Gunardi Haswinzky, Rose Amalia Heri Wibowo Herkutanto Hutabarat, Hernayati Ika Anggraini Indina Sastrini Inge Ade Krisanti Irawan, Yudo Irene Dorthy Santoso Jacoeb, Tjut N.A. Jhauharina Rizki Fadhilla Joedo Prihartono Kayika, I Putu Gede Kemal N. Siregar Krisanti, Inge Ade Krisanti, Roro Inge Ade Kuraga, Rahmaniar Desianti Kurniawan, Kristian Kusmarinah Bramono Larisa Paramitha Leonardo Lesmana, Elvira Lidwina Anissa Lili Legiawati Manalu, Patricsia Marissa, Melani Martinus Tarina Martinus, Martinus Mawardi, Prasetya Melani Marissa Melani Marissa Nelson, Benny Nur Azizah, Anggita Nurhadi Ibrahim Patricsia Manalu Peppy Fourina Pusponegoro, Erdina H.D. Rahadi Rihamadja Rahadi Rihatmadja Rahadi Rihatmadja Rahmaniar Desianti Kuraga Santoso, Irene Dorthy Sastrini, Indina Satya Wydya Yenny Shannaz Nadia Yusharyahya Sirait, Sondang P. Siti Nurani Fauziah Siti Setiati Sondang Pandjaitan Sirait Sri L. Menaldi Sugiharto, Ade Firmansyah Surahman Hakim Suyatna, Fransiscus Dhyanagiri Wahyu Widodo Widjajalaksmi Kusumaningsih, Widjajalaksmi Widya Artini Windy Keumala Budianti Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Yeva Rosana Yudo Irawan Yudo Irawan Zainal Hakim