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All Journal Jurnal Ilmiah Mahasiswa FKIP Prodi Biologi International Journal of Economics, Business and Accounting Research (IJEBAR) Jurnal Perseda : Jurnal Pendidikan Guru Sekolah Dasar Jurnal Ilmiah Biologi UMA (JIBIOMA) BioEksakta : Jurnal Ilmiah Biologi Unsoed Al-fahim : Jurnal Manajemen Pendidikan Islam Indonesian Values and Character Education Journal KIDDO: Jurnal pendidikan Islam Anak Usia Dini Albariq: Jurnal Pendidikan Bahasa Arab SOCIAL : Jurnal Inovasi Pendidikan IPS Prosiding Seminar Nasional Pendidikan Matematika, Universitas Mulawarman Jurnal Arsitektur, Sains Bangunan, Kota dan Pemukiman Innovative: Journal Of Social Science Research Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Gudang Jurnal Multidisiplin Ilmu JIM: Jurnal Ilmiah Mahasiswa Pendidikan Sejarah Indonesian Proceedings and Annual Conference of Islamic Law And Sharia Economic (IPACILSE) El-Mujtama: Jurnal Pengabdian Masyarakat Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Penelitian Multidisiplin Bangsa BIO-SAINS | Jurnal Ilmiah Biologi Journal of English Teaching and Learning LA RIBA: Jurnal Perbankan Syariah
Siti Maysaroh
Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Pasir Pengaraian
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Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Nilai-Nilai Islam dalam Ruang Lingkup Adat Istiadat Betawi Al Fahmi, Faiz Fikri; Aisah, Siti; Maysaroh, Siti; Edilah, Selvi; Adawiyah, Robiatul
El-Mujtama: Jurnal Pengabdian Masyarakat  Vol. 5 No. 2 (2025): El-Mujtama: Jurnal Pengabdian Masyarakat
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/elmujtama.v5i2.6546

Abstract

The Betawi community, which is an ethnic gather with a wealthy and different culture. Zakat, which comes from a dialect meaning develop and develop, not as it were serves to extend the compensate for those who carry it out, but too to freed oneself of sin. Within the setting of sharia, zakat plays an critical part in making a difference individuals in require and making social welfare. This ponder employments a subjective expressive strategy to ponder Islamic values in Betawi instruction, as well as how social differences can make questions between devout communities. The Betawi community, as a result of the societies of different tribes, appears that conventional relational unions have a central part in building concordant social relations. Marriage is considered a blessing from God that ties men and ladies to live together in joy and success. In this setting, this article moreover highlights the significance of resilience and concern in a assorted society, in agreement with Islamic lessons that emphasize that contrasts are sunnatullah that must be acknowledged and regarded. Through an in-depth literature review, the creator looks for to extend his understanding of this point, within the trust of contributing to advance investigate on social interaction and devout values in Indonesian society. This inquire about is anticipated to be a reference for future considers related to devout information and the part of zakat in a different society.
TOROSIAJE MANGROVE ECOPARK DI KABUPATEN POHUWATO DENGAN PENDEKATAN ARSITEKTUR EKOLOGI Maysaroh, Siti; Siola, Amru; Eka, Rahmawati
Venustas Vol 2 No 2 (2023): Mei
Publisher : Program Studi Teknik Arsitektur, Fakultas Teknik - Universitas Ichsan Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37195/venustashome.v2i2.423

Abstract

Perancangan  ini bertujuan untuk merancang sebuah wisata mangrove ecopark yang  ada  di  Torosiaje Kabupaten Pohuwato  dengan  menggunakan sebuah  pendekatan Arsitektur Ekologi.   Wisata merupakan sebuah kegiatan yang dilakukan seseorang atau sekelompok orang untuk melepas penat atau berekreasi dan bersantai. Wisata juga menjadi salah satu kegiatan yang dapat membantu peningkatan pendapatan daerah. Adapun penelitian pada perancangan ini dilakukan dengan cara observasi, dokumentasi dan studi kepustakaan. Dari hasil data-data yang ada  kemudian menghasilkan sebuah rancangan kawasan wisata mangrove. Adapun tahap yang dilakukan sebelum perancangan yaitu pembuatan konsep pendekatan yang di gunakan agar kemudian akan menghasilkan sebuah gambar perancangan Mangrove Ecopark. Adapun pendekatan yang di gunakan yaitu pendekatan ekologi yang bertujuan agar pembangunan Ecopark tidak merusak lingkungan dan menggunakan material yang dapat di perbaharui sehingga tidak menyebabkan pencemaran pada lingkungan. Penerapan Arsitektur Ekologi pada perancangan terletak pada penataan bangunan yang berada di antara pepohonan mangrovesehingga nantinya tidak merusak kawasan mangrove yang ada.
EMPLOYING MIND MAPPING TECHNIQUE TO FACILITATE SECONDARY SCHOOL STUDENT TO WRITE NARRATIVE TEXT AT THE FIRST GRADE IN THE 2020/2021 ACADEMIC YEAR Maysaroh, Siti
Journal of English Teaching and Learning Vol 1 No 01 (2021): Journal of English Teaching and Learning
Publisher : Faculty of Teaching and Education, University of August 17, 1945, Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Employing Mind Mapping Technique to Facilitate Secondary School Student to Write Narrative Text At The First Grade In the 2020/2021 Academic Year. This research was conducted on class XI students of SMA Negeri 1 Wongsorejo Banyuwangi. This research used Class Action Research with research design using Pre-Test and Post-Test in cycle 1 and cycle 2. The data of this study were in the form of student scores both pre-test and post-test. The data collection technique was carried out by researchers by collecting student scores, observation sheets on student activities both before research, and after research, interviews with students and teachers on learning before and after using the mind mapping method. The results showed that: learning using the mind mapping method was classified as very supportive of students' abilities in learning writing narrative text. This is evidenced by the increase in student test results from cycle 1 with an average result of 72.79 students with a completeness level of 59%, increasing in cycle 2 with an average value of 79.26 with a completeness level reaching 85%. Student responses to learning using the mind mapping method are very happy, and students are quite active in group learning activities. In cycle 1 students do individual learning activities but the results are still not optimal because students still do not understand the mind mapping method, but in cycle 2 with group learning students experience an increase in ability because students can discuss with their group friends.
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Co-Authors afiyah, nur Agus Susanti Ajiza, Pratisa Deifirera Al Fahmi, Faiz Fikri Alia, Rahmah Amru Siola Anggraini, Nurlita Anggraini, Nurlita Putri Annisa Siregar*, Nabilla Asnawi, Felisa Asnawi, Muhammad Hasan Azizah, Sarah Balqis Azzahra Beginer Subhan Cindya Alfi Dzikri Alhamda Siregar Edilah, Selvi Eka, Rahmawati Elvira Elvira Faizah, Intan Nur Fendy Hardian Permana Firdausiyah, Nuri Fitria, Azijatul Hairunnisa Harto Nuroso Heru Juabdin Sada Indriani, Amanda Juhji, Juhji Jumrodah Jumrodah, Jumrodah Karlina, Siti Khairotunnisa, Maya Lestari, Eka Dwi Magfiroh, Siti Aisyatul Melati Meutia Samira Ismet Mohamad Fatih Muh.Amin, Astuti MUHAMMAD ALI Musrifa, Musrifa Najhah, Nor Rezqiyatun Nasution, Fatimatuzzahra Nikmah, Himayatin Nirmalasari, Ridha Noor Hujjatusnaini Nuridah, Nuridah Nuriyah, Sinta Nurjannah, Eka Nurrohmah, Anissa Ika Pitria, Pitria Putri Yasnu, Anjani Rayani Sinuhaji, Tri nanda Reski Saputra, Reski Respi, Respi Riki Riharji Lubis, Riki Riharji RITA ANDINI Robiatul Adawiyah Rofiza Yolanda, Rofiza Rohmaniyah, Ismatur Rosida, Anis Rr. Eko Susetyarini Salsabila, Chairunisa Samira Ismet, Meutia Satria Kurniawan Sembiring, Enny Rimita Siti Aisah Sofiana, Nadia Nurcahyani Sofiyah Sofiyah Sugianto Sugianto Sulistyowati, Ayu Sunan Baedowi, Sunan Ulida, Ulida Umi Prihatini , Hikmah Virnanda, Irma Winda Sherly Utami Yetti Elfina Yuliyana, Via