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Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Nilai-Nilai Islam dalam Ruang Lingkup Adat Istiadat Betawi Al Fahmi, Faiz Fikri; Aisah, Siti; Maysaroh, Siti; Edilah, Selvi; Adawiyah, Robiatul
El-Mujtama: Jurnal Pengabdian Masyarakat  Vol. 5 No. 2 (2025): El-Mujtama: Jurnal Pengabdian Masyarakat
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/elmujtama.v5i2.6546

Abstract

The Betawi community, which is an ethnic gather with a wealthy and different culture. Zakat, which comes from a dialect meaning develop and develop, not as it were serves to extend the compensate for those who carry it out, but too to freed oneself of sin. Within the setting of sharia, zakat plays an critical part in making a difference individuals in require and making social welfare. This ponder employments a subjective expressive strategy to ponder Islamic values in Betawi instruction, as well as how social differences can make questions between devout communities. The Betawi community, as a result of the societies of different tribes, appears that conventional relational unions have a central part in building concordant social relations. Marriage is considered a blessing from God that ties men and ladies to live together in joy and success. In this setting, this article moreover highlights the significance of resilience and concern in a assorted society, in agreement with Islamic lessons that emphasize that contrasts are sunnatullah that must be acknowledged and regarded. Through an in-depth literature review, the creator looks for to extend his understanding of this point, within the trust of contributing to advance investigate on social interaction and devout values in Indonesian society. This inquire about is anticipated to be a reference for future considers related to devout information and the part of zakat in a different society.
TOROSIAJE MANGROVE ECOPARK DI KABUPATEN POHUWATO DENGAN PENDEKATAN ARSITEKTUR EKOLOGI Maysaroh, Siti; Siola, Amru; Eka, Rahmawati
Venustas Vol 2 No 2 (2023): Mei
Publisher : Program Studi Teknik Arsitektur, Fakultas Teknik - Universitas Ichsan Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37195/venustashome.v2i2.423

Abstract

Perancangan  ini bertujuan untuk merancang sebuah wisata mangrove ecopark yang  ada  di  Torosiaje Kabupaten Pohuwato  dengan  menggunakan sebuah  pendekatan Arsitektur Ekologi.   Wisata merupakan sebuah kegiatan yang dilakukan seseorang atau sekelompok orang untuk melepas penat atau berekreasi dan bersantai. Wisata juga menjadi salah satu kegiatan yang dapat membantu peningkatan pendapatan daerah. Adapun penelitian pada perancangan ini dilakukan dengan cara observasi, dokumentasi dan studi kepustakaan. Dari hasil data-data yang ada  kemudian menghasilkan sebuah rancangan kawasan wisata mangrove. Adapun tahap yang dilakukan sebelum perancangan yaitu pembuatan konsep pendekatan yang di gunakan agar kemudian akan menghasilkan sebuah gambar perancangan Mangrove Ecopark. Adapun pendekatan yang di gunakan yaitu pendekatan ekologi yang bertujuan agar pembangunan Ecopark tidak merusak lingkungan dan menggunakan material yang dapat di perbaharui sehingga tidak menyebabkan pencemaran pada lingkungan. Penerapan Arsitektur Ekologi pada perancangan terletak pada penataan bangunan yang berada di antara pepohonan mangrovesehingga nantinya tidak merusak kawasan mangrove yang ada.
EMPLOYING MIND MAPPING TECHNIQUE TO FACILITATE SECONDARY SCHOOL STUDENT TO WRITE NARRATIVE TEXT AT THE FIRST GRADE IN THE 2020/2021 ACADEMIC YEAR Maysaroh, Siti
Journal of English Teaching and Learning Vol 1 No 01 (2021): Journal of English Teaching and Learning
Publisher : Faculty of Teaching and Education, University of August 17, 1945, Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Employing Mind Mapping Technique to Facilitate Secondary School Student to Write Narrative Text At The First Grade In the 2020/2021 Academic Year. This research was conducted on class XI students of SMA Negeri 1 Wongsorejo Banyuwangi. This research used Class Action Research with research design using Pre-Test and Post-Test in cycle 1 and cycle 2. The data of this study were in the form of student scores both pre-test and post-test. The data collection technique was carried out by researchers by collecting student scores, observation sheets on student activities both before research, and after research, interviews with students and teachers on learning before and after using the mind mapping method. The results showed that: learning using the mind mapping method was classified as very supportive of students' abilities in learning writing narrative text. This is evidenced by the increase in student test results from cycle 1 with an average result of 72.79 students with a completeness level of 59%, increasing in cycle 2 with an average value of 79.26 with a completeness level reaching 85%. Student responses to learning using the mind mapping method are very happy, and students are quite active in group learning activities. In cycle 1 students do individual learning activities but the results are still not optimal because students still do not understand the mind mapping method, but in cycle 2 with group learning students experience an increase in ability because students can discuss with their group friends.
The Main Character's Inner Conflict in the Novel Ayah, Berjuang Sendiri Itu Capek by Adellia PD: A Psychoanalytic Perspective : Maysaroh, Siti
Jurnal Pembelajaran Bahasa dan Sastra Vol. 5 No. 1 (2026): Januari 2026
Publisher : Raja Zulkarnain Education Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55909/jpbs.v5i1.1223

Abstract

This study aims to analyze the inner conflict of the main character, Brichia, in the novel Ayah, Berjuang Sendiri Itu Capek! by Adellia P.D. (2025). This inner conflict arises due to the conflict between financial and moral responsibilities towards the family and the character’s desire to achieve her dream of becoming a doctor. This study uses a qualitative descriptive method with a literary psychology approach, specifically Sigmund Freud’s psychoanalytic theory which includes the personality structure of the id, ego, and superego as well as self-defense mechanisms. The main data source of this study is the novel Ayah, Berjuang Sendiri Itu Capek! published in 2025. Supporting data is obtained from reference books, scientific articles, and relevant research related to literary psychology. Data collection techniques are carried out through intensive reading and recording data in the form of narrative excerpts and dialogues that represent the character’s inner conflict. Data analysis is carried out by classifying data based on aspects of the id, ego, superego, and forms of self-defense mechanisms. The results of the study indicate that Brichia’s inner conflict is caused by the dominance of the id that drives the fulfillment of personal ambition, the superego that instills moral values and a sense of responsibility towards the family, and the role of the ego that tries to mediate these two drives through rational actions, such as seeking realistic educational alternatives. To reduce psychological stress, Brichia uses self-defense mechanisms, including repression, projection, and sublimation. This inner conflict contributes to the formation of Brichia’s character as an independent, tough, and principled woman.Keywords: inner conflict, Sigmund Freud’s psychoanalysis, id, ego, superego, Father, Fighting Alone Is Tiring!
STRATEGI KEPEMIMPINAN KEPALA MADRASAH DALAM MENGEMBANGKAN BUDAYA RELIGIUS DI MTs NEGERI 1 PURWOREJO Maysaroh, Siti; Musolin, Muhlil; Khasanah, Fitrohtul
Jurnal Manajemen Pendidikan Vol. 11 No. 1 (2026): Regular Issue (In Progress)
Publisher : STKIP Pesisir Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34125/jmp.v11i1.1711

Abstract

The decline in internalization of religious values among students as a result of digital culture. Madrasahs require strong leadership strategies to overcome the impact of digital culture, especially to foster a strong religious environment. The purpose of this study was to determine the leadership strategies of madrasah principals in developing a religious culture and the inhibiting and supporting factors at MTs Negeri 1 Purworejo. This study used a qualitative methodology with a phenomenological approach. The research subjects included madrasah principals, religious teachers, and students. Data collection techniques were carried out through in-depth interviews, observation, and documentation. Data analysis used the Miles and Huberman model which includes data reduction, data presentation, and drawing conclusions. The results showed that the madrasah principal's strategy plays a role as an educator, manager, administrator, supervisor, leader, innovator, and motivator in building a religious culture in madrasahs. The strategies implemented include organizing religious activities, continuous supervision and monitoring, appreciation for teachers and students, and coaching for students. Supporting factors for the success of religious culture include support from all madrasah members and adequate worship facilities, while inhibiting factors are the influence of digital culture and limited time for religious activities.
Sosialisasi Sistem Depurasi Kerang dan Keamanan Pangan Produk Kerang kepada Taruna/i Lintas Program Studidi Politeknik Kelautan dan Perikanan Sorong Maurrenzha Leatemia, Brigitha; Lestariaji, Chandika; Maysaroh, Siti; Lady First Bara, Grace; Hafiizh Tantr, Ashr; Rachmawan Tantri, Arnest; Tantri, Akbar Falah
Jurnal Pengabdian Perikanan Indonesia Vol 6 No 1 (2026): Jurnal Pengabdian Perikana Indonesia
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppi.v6i1.9276

Abstract

Abstrak: Kerang merupakan komoditas perikanan bernilai ekonomi tinggi namun berpotensi menimbulkan risiko kesehatan akibat akumulasi mikroorganisme patogen, logam berat, dan biotoksin laut. Salah satu upaya mitigasi risiko keamanan pangan adalah penerapan sistem depurasi kerang sebelum dikonsumsi. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan dan pemahaman taruna/i lintas program studi di Politeknik Kelautan dan Perikanan Sorong mengenai sistem depurasi kerang dan prinsip keamanan pangan produk kerang. Metode pelaksanaan meliputi sosialisasi, ceramah interaktif, diskusi, serta evaluasi menggunakan pre-test dan post-test. Hasil kegiatan menunjukkan adanya peningkatan pemahaman peserta terhadap konsep depurasi, faktor kontaminasi kerang, serta implikasinya terhadap kesehatan manusia. Kegiatan ini berkontribusi dalam penguatan kapasitas sumber daya manusia sektor kelautan dan perikanan untuk mendukung pengelolaan produk perikanan yang aman dan berkelanjutan.
Co-Authors afiyah, nur Agus Susanti Ajiza, Pratisa Deifirera Al Fahmi, Faiz Fikri Alia, Rahmah Amru Siola Anggraini, Nurlita Anggraini, Nurlita Putri Annisa Siregar*, Nabilla Asnawi, Felisa Asnawi, Muhammad Hasan Azizah, Sarah Balqis Azzahra Beginer Subhan Cindya Alfi Dzikri Alhamda Siregar Edilah, Selvi Eka, Rahmawati Elvira Elvira Faizah, Intan Nur Fendy Hardian Permana Firdausiyah, Nuri Fitria, Azijatul Hafiizh Tantr, Ashr Hairunnisa Harto Nuroso Heru Juabdin Sada Indriani, Amanda Juhji, Juhji Jumrodah Jumrodah, Jumrodah Karlina, Siti Khairotunnisa, Maya Khasanah, Fitrohtul Lady First Bara, Grace Lestari, Eka Dwi Lestariaji, Chandika Magfiroh, Siti Aisyatul Maurrenzha Leatemia, Brigitha Melati Meutia Samira Ismet Mohamad Fatih Muh.Amin, Astuti MUHAMMAD ALI Musolin, Muhlil Musrifa, Musrifa Najhah, Nor Rezqiyatun Nasution, Fatimatuzzahra Nikmah, Himayatin Nirmalasari, Ridha Noor Hujjatusnaini Nuridah, Nuridah Nuriyah, Sinta Nurjannah, Eka Nurrohmah, Anissa Ika Pitria, Pitria Putri Yasnu, Anjani Rachmawan Tantri, Arnest Rayani Sinuhaji, Tri nanda Reski Saputra, Reski Respi, Respi Riki Riharji Lubis, Riki Riharji RITA ANDINI Robiatul Adawiyah Rofiza Yolanda, Rofiza Rohmaniyah, Ismatur Rosida, Anis Rr. Eko Susetyarini Salsabila, Chairunisa Samira Ismet, Meutia Satria Kurniawan Sembiring, Enny Rimita Siti Aisah Sofiana, Nadia Nurcahyani Sofiyah Sofiyah Sugianto Sugianto Sulistyowati, Ayu Sunan Baedowi, Sunan Tantri, Akbar Falah Ulida, Ulida Umi Prihatini , Hikmah Virnanda, Irma Winda Sherly Utami Yetti Elfina Yuliyana, Via