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All Journal Indonesian Journal of Tropical and Infectious Disease The Indonesian Biomedical Journal Sari Pediatri Jurnal Teknologi Laboratorium THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Care : Jurnal Ilmiah Ilmu Kesehatan JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Journal of Livestock and Animal Health (JLAH) Jurnal Medis Umum Folia Medica Indonesiana
Hartono Kahar, Hartono
Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN Agustiningrum, Indah; Nugraha, Jusak; Kahar, Hartono
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 1 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i1.12696

Abstract

Dengue infection caused by DENV and transmitted by mosquitoes Aedes aegypti and Aedes albopictus is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection are very widely, from asymptomatic until dengue shock syndrome (DSS). DENV will attack macrophages and dendritic cells (DC) and replicate them. Monocytes are macrophages in the blood (±10% leukocytes). Macrophages produce cytokines and chemokines such as monocyte chemotactic protein-1 (MCP-1)/CCL2. The monocytes that are infected with DENV will express MCP-1, which will increase the permeability of vascular endothelial cells so that they have a risk of developing DHF/DSS. Macrophages and DC secrete NS1 proteins, which are the co-factors that are needed for viral replication and can be detected in the early phase of fever. The increased MCP-1 levels in dengue infection followed by an increase in the number of atypical lymphocytes indicate the arrival of macrophages and monocytes to the site of inflammation which triggers proliferation rather than lymphocytes. This is an observational analytical study with a cross-sectional design to determine the MCP-1 level in dengue infection patients with 1st until the 4th day of fever and the presence of atypical lymphocytes. Dengue infection was determined by rapid tests NS1 positive or negative and MCP-1 levels were measured using by ELISA sandwich method.MCP-1 level of sixty patients dengue infection NS-1 rapid positive or negative with 2nd until 4rt fever were significantly higher than healthy subjects (420.263±158,496vs29, 475±23.443;p=0.000), but there was no significant difference in subjects with DF, DHF or DSS (436,47±225,59 vs422,77±170,55vs 448,50±117,39; p =0.844). Atypicallymphosite differs significantly in healthy  subjects than subjects infected with DENV an average of 2% (p= 0,000). In conclusion, this shows the arrival of macrophages and monocytes to the site of inflammation, which triggers the proliferation of lymphocytes.
Hyperbaric Oxygen Therapy Increases Brain-Derived Neurotrophic Factor as well as Decreases Systemic Immune-Inflammatory Index and Systemic Inflammatory Response Index in Autism Spectrum Disorder Verna Biutifasari; Ni Komang Sri Dewi Untari; Pramita Anindya Nugraheni; Ronald Pratama Adiwinoto; Djatiwidodo Edi Pratiknya; Vendra Setiawan; Hartono Kahar; Betty Agustina Tambunan
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3358

Abstract

BACKGROUND: Neuroinflammation and immune dysregulation are frequently viewed as contributing factors of autism spectrum disorder (ASD). Brain-derived neurotrophic factor (BDNF) is involved in the maintenance of neuron viability, as well as in neuron differentiation. Meanwhile, Systemic Immune-Inflammatory Index (SII) and Systemic Inflammatory Response Index (SIRI) are basic hematological indices used to assess inflammation and immune status. Hyperbaric oxygen (HBO) is known to enhance cerebral blood flow and reduce inflammation, however, not many studies have observed the its effect on BDNF level, SII, and SIRI in ASD subjects; therefore, this study was performed.METHODS: Fifteen ASD subjects were involved in this study and received HBO therapy 10 times within a 2-week period. The HBO therapy was performed by letting the subjects got into an isolated chamber filled with 100% oxygen and 1.3 ATA pressure for 60 minutes. Pre- and post-therapy blood samples were taken from subjects. BDNF level was measured with Enzyme Linked Immunosorbent Assay (ELISA), while neutrophils, monocytes, lymphocytes and platelets were measured by hematology analyzer for the calculation of SII and SIRI.RESULTS: Post-therapy BDNF level was higher than pre-therapy (1.84 ng/mL vs. 1.46 ng/mL; p=0.039). The increased in BDNF suggested reduced neuroinflammation and enhanced connections between neurons. Both post-therapy SII (672.4 vs. 359.4; p=0.005) and SIRI (1.3 vs. 0.7; p=0.009) were significantly lower than pre-therapy indexes. Decreased in SII and SIRI signified a reduction in neuroinflammation.CONCLUSION: HBO therapy increases BDNF level, also decreases SII and SIRI in ASD subjects. These results suggest that HBO has an effect on neuroinflammation, specifically in ameliorating inflammation.KEYWORDS: autism spectrum disorder, BDNF, SII, SIRI, hyperbaric oxygen therapy
Identifikasi Antigen Eritrosit Kucing Domestik dan Kucing Persia dengan Menggunakan Metode Imunokromatografi: Identification of Domestic and Persian Cat Erythrocyte Antigens Using Immunochromatography Method Palestin; Wardhani, Hana; Hermawan, Intan Permatasari; Kahar, Hartono; Dachlan, Yus Prijatna; Zelpina, Engki
Journal of Livestock and Animal Health Vol. 8 No. 2 (2025): August
Publisher : Politeknik Pertanian Negeri Payakumbuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32530/jlah.v8i2.89

Abstract

Identifikasi antigen eritrosit pada kucing penting dilakukan. Hal ini bertujuan untuk mengetahui golongan darah pada kucing yang merupakan salah satu prosedur  wajib jika seekor kucing memerlukan transfusi darah pada keadaan tertentu, misalnya kucing yang menderita kanker, anemia, pendarahan akut atau kronis, serta infeksi virus. Selain untuk berbagai permasalahan tersebut, identifikasi antigen eritrosit juga penting dilakukan untuk mencegah kasus isoeritrolisis neonatus pada anak kucing akibat perkawinan jantan dan induk yang berbeda jenis antigen eritrositnya. Sistem penggolongan darah kucing didasarkan pada perbedaan antigen eritrosit yang membentuk tiga tipe utama, yaitu A, B, dan AB. Golongan darah A merupakan yang paling dominan, ditemukan pada sekitar 95% populasi kucing global, sementara tipe B dan AB tergolong langka. Secara biokimiawi, antigen pada tipe A dan B merupakan gangliosida berbeda dengan berat molekul 50 kD - tipe A hanya mengekspresikan NeuGc, sedangkan tipe B secara eksklusif mengandung NeuAc. Adapun tipe AB mengekspresikan kedua komponen (NeuAc dan NeuGc) dalam proporsi seimbang.
POLA JUMLAH TROMBOSIT PENDERITA DEWASA TERINFEKSI VIRUS DENGUE DI RSUD DR.SOETOMO SURABAYA Afgriyuspita, Lelyana Sih; Kahar, Hartono; Sudiana, I Ketut
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 1 (2021): EDITION MARCH 2021
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i1.1365

Abstract

ABSTRACT The trend of dengue viral infection in Indonesia and several countries has changed from children to older age groups. Thrombocytopenia is a common laboratory parameter used to diagnose dengue virus infection that has the possibility to change day by day. This research was expected to improve understanding thrombocyte pattern in adult patients. This was a descriptive study with a retrospective design using medical records of Dr. Soetomo general hospital Surabaya during 2017-2018. The sample used was adult patients in the age range of 18-55 years old with Dengue Fever (DF) or Dengue Haemorrhagic Fever (DHF). The sampling technique was total sampling and obtained 62 samples. Data was analyzed using an independent t-test and Mann-Whitney. The pattern of average thrombocyte count decreased on the 3rd day till the 6th day. Entering the 7th day, it increased but did not reach the normal value. There were significant differences in the average of thrombocyte count between DF and DHF patients during the 4th day (p=0.003), 5th day (p = 0.006) and the 6th day (p= 0.014). Decreased thrombocyte count < 100,000/µL in DHF patients should be intensively observed starting from the 3rd day of illness. Keywords : DF,DHF, thrombocyte,adult
Transformasi Patologi Klinik melalui Kecerdasan Buatan: Sebuah Tinjauan Pustaka Sistematis Ambar, Nabil Salim; Utama, Muhamad Reza; Kahar, Hartono
JurnalMU: Jurnal Medis Umum Vol 1 No 3 (2024): Jurnal Medis Umum
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmu.v1i3.24771

Abstract

Kecerdasan buatan (Artificial Intelligence/AI) telah mengalami perkembangan signifikan dalam bidang patologi klinik selama dekade terakhir. AI mendukung analisis data kompleks dalam histopatologi, meningkatkan efisiensi diagnosis, dan mempercepat pengambilan keputusan klinis. Artikel ini bertujuan untuk mengulas peran AI dalam patologi klinik, termasuk penerapannya dalam diagnostik, prediksi klinis, dan personalisasi pengobatan. Metode yang digunakan adalah tinjauan pustaka sistematis dengan mencakup literatur dari database utama dalam 10 tahun terakhir. Hasil menunjukkan bahwa AI mampu meningkatkan akurasi diagnostik hingga 96,3% dan spesifisitas hingga 93,3%, serta mempercepat workflow klinis. Meskipun demikian, terdapat tantangan seperti regulasi, etika, dan kesenjangan digitalisasi yang perlu diatasi. AI menawarkan peluang besar untuk transformasi patologi klinik menuju era pengobatan presisi.
Effect of Long-Term Ketogenic Diet in Mice Serum Adiponectin Kurniasari, Hamidah; Rejeki, Purwo Sri; Kahar, Hartono; Sunarti, Sri
Folia Medica Indonesiana Vol. 58, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlight: • Adiponectin is an important protective factor for cardiovascular disease and increased insulin sensitivity. • Ketogenic diet effect on adiponectin level in mice besides decrease weight gain was determined. • The difference in serum adiponectin level and body weight is uncorrelated. Abstract: Ketogenic diet is a popular diet to reduce weight gain quickly. This diet has become a lifestyle . The ketogenic diet has been reported to affect adiponectin level, although it is still contraindicated. Adiponectin is a biomarker for metabolic disease that plays an important role as a protective factor for cardiovascular disease and increase insulin sensitivity. This study aimed to explore the effect of the ketogenic diet on adiponectin level in mice, besides decrease weight gain. This study was an experimental laboratory study with a randomized post-test-only control group design. Fourteen male mice (20-30 g) aged 2-3 months were divided randomly into K1 (n=7, standard diet) and K2 (n=7, ketogenic diet), were given diet for eight weeks, ad libitum. Body weight was measured at the pre and post-intervention, whereas adiponectin level were measured at the post-intervention using ELISA. The statistical analysis using SPSS version 16. The Shapiro Wilk test was used to determine normality, Independent T-Test was used to determine mean difference for normal distribution, Mann Whitney Test was used to determine mean difference for abnormal distribution, and Pearson Correlation was used to determine correlation. Difference (∆) of body weight on K1 (12.00±6.26) g, K2 (1.29±7.41) g with p=0.02. Serum adiponectin level of K1 (0.082±0.014) µg/ml and K2 (0.096±0.008) µg/ml with p=0.035. This study showed ketogenic diet-induced higher serum adiponectin level and slower weight gain. There is no correlation between the difference in body weight and serum adiponectin level (p=0.403).
Immunogenomic Phase-Based Differences in Complete Blood Count Profiles of COVID-19 Patients With and Without Comorbidities Yusuf, Ratu Diva Nuralifia; Rusli, Musofa; Kahar, Hartono
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 17 No. 1 (2026): JANUARY 2026 (IN-PROGRESS ISSUE)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V17I12026.22-28

Abstract

Highlights: Complete blood count profile represents the most frequently conducted laboratory test, pivotal for prognostic assessment, particularly in COVID-19 patients presenting with comorbidities. Significant differences exist within CBC profiles across various immunogenomic phases, notably leukocyte count, thrombocyte count, and NLR.   Abstract Introduction: The coronavirus disease (COVID-19) pandemic has resulted in significant mortality in Indonesia, particularly affecting those with comorbidities. Utilizing complete blood count (CBC) profiles can inform clinical management strategies and enhance patient care. This study examined the correlations among CBC profiles, immunogenomic phase, and disease pathophysiology. Methods: This was a cross-sectional, retrospective, observational study that used medical records from Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Demographic data from 134 COVID-19 patients were analyzed, focusing on differences in CBC profiles between patients with and without comorbidities across immunogenomic phases. Statistical comparisons were conducted using the International Business Machines Corporation (IBM) Statistical Package for the Social Sciences (SPSS) version 13, with significance set at p<0.05. Results: Most patients were male, with a mean age of 52.13 years old. Cardiovascular comorbidities were prevalent (36%) across all immunogenomic phases. Significant differences in leukocyte and platelet counts were observed among patients with comorbidities. The neutrophil-to-lymphocyte (NLR) ratio varied significantly between immunogenomic phases in both comorbid and non-comorbid groups. Additionally, significant variations in leukocyte and platelet counts were found in the comorbid group, along with differences in NLR in both groups (p<0.05). Conclusion: Variations in leukocyte count, platelet count, and NLR indicated that comorbidities and CBC profiles might contribute to the development of cytokine storm, acute respiratory distress syndrome, and multiple organ failure.
Co-Authors Afgriyuspita, Lelyana Sih Agustiningrum, Indah Ambar, Nabil Salim Arifoel Hajat Betty Agustina Tambunan Biutifasari, Verna Dachlan, Yus Prijatna Djatiwidodo Edi Pratiknya Dwiyanti Puspitasari, Dwiyanti Elly Sulistyani Engki Zelpina Hamidah Kurniasari Harida Zahraini Heny Arwati Hermawan, Intan Permatasari Hildegardis Dyna Dumilah Jelita Aprisano Putri Jusak Nugraha Kasiyati, Menik Kurniasari, Hamidah Kusuma, Edward Putra Lelyana Sih Afgriyuspita M. Robiul Fuadi Malfira Iswary Muhamad Reza Utama Nabilla Feirizky Chairunnisa Ni Komang Sri Dewi Untari Nur Vita Purwaningsih, Nur Vita Palestin Pramita Anindya Nugraheni Rahma Widyastuti Rejeki, Purwo Sri Rizkiya Candra Sari Ronald Pratama Adiwinoto Rusli, Musofa S.Pd. M Kes I Ketut Sudiana . Setiawan, Philia Shinta Lungit Ambaringrum Soebroto, Heroe Sri Sunarti Sri Sunarti Tito Yustiawan Tri Ade Saputro Vella Rohmayani Vendra Setiawan Waras Budiman Wardhani, Hana Yetti Hernaningsih Yulia Nadar Indrasari Yusuf, Ratu Diva Nuralifia Zubir Zubir