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Journal : bionature

Isolasi dan Uji Bioaktivitas Senyawa Metabolit Sekunder Ekstrak N-Heksan Daun Pare (Momordica charantia L.) Muharram, .
bionature Vol 11, No 2 (2010): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.866 KB) | DOI: 10.35580/bionature.v11i2.1380

Abstract

Bittermelon (Momordica charantia L.) is a bioactivity plant that used as a medicine traditional. The purpose of this research is to isolate the bioactivity secondary metabolites of n-hexane extract from the bittermelon leaves and bioassay toward larval shrimp A. salina. Isolation of the secondary metabolites including extraction, fractionation, purification, and identification. Bioassay was conducted to the extract and to mixed fractions (fraction A – G) the results of column chromatography. The results of research showed that the extracts and fractions from bittermelon leaves was toxic to A. salina. The LC50 from extracts bittermelon leaves was 91, 84 ppm. The LC50 for the fractions A and B (steroid) were 38,26 ppm and  85,90 ppm, the fractions C – G (terpenoid) were 94,45 ppm; 79,56 ppm; 92,53 ppm; 70,41 ppm; and 92,69 ppm, the most toxic fractions was fraction of A. From the C-fraction as a green coulor was obtained a crystal and needle-shaped. After recystalization with n-hexane and ethylacetat was obtained a pure compound that positive to terpenoids with Liebermann-Burchard reagents. 
Isolasi dan Identifikasi Sterol dari Ekstrak n-heksana Daun Meniran Hijau Phyllanthus niruri L. (Euphorbiaceae) Muharram, .; Jannah, Nur
bionature Vol 10, No 2 (2009): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.286 KB) | DOI: 10.35580/bionature.v10i2.291

Abstract

One of the secondary metabolite, namely a sterol had been iosolated from the n-hexane extract of Phyllanthus niruri L leaves. This compound was obtained by isolation that consist of extaction, fractionation, purification, and identification. The identification covers the melting point test, thin layer chromatography and Liebermann-Burchard test. The research results show that the compound is white needle crystal with the malting point 120-122⁰C and positive with Lieberman-Burchard reagens that give blue-green coulor. This compound have not yet been established, because it must be evaluated by spectroscopy.methods.   Keywords: isolation, Phyllanthus niruri L., sterol, Liebermann-Burchard reagent, spectroscopy method
POTENSI EKSTRAK TUMBUHAN TEMBELEKANG (LANTANA CAMARA LINN.) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI. Iwan, Dini; Muharram, .; Sitti, Faika
bionature Vol 12, No 1 (2011): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.675 KB) | DOI: 10.35580/bionature.v12i1.1391

Abstract

The extract of leaf, fruit and tree bark of tembelekang (L. camara Linn.) as antibacterial activity to Staphylococcus aureus and Escherichia coli has been studied using agar diffusion method. The extract producted with extraction with maseration fraction teqnique. The aim of this research is to show the potential L. camara plant as antibacterial to against Staphylocuccus aureus and Escherichia coli. The research result showed that extract of leaf of L. camara plant having potency as antibacterial and showing that there is a real correlation between the L. camara plant as the medicinal plant and the bioactive compounds contained in it. 
Daya Hambat Ekstrak Metanol Daun Sukun (Artocarpus communis Forst) terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus Penyebab Infeksi pada Luka Muharram, .; Ramdani, .; Eviyusmiawati Sius, .
bionature Vol 9, No 2 (2008)
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v9i2.112

Abstract

This research is aimed to know the inhibiting capacity of methanol extact of Artocarpus communis Forst leaves to bacteria that causing infection on wound, i.e. P. aeruginosa and S. Aureus. Therefore, the sensitivity of both bacteria must be examined to methanol extract of Artocarpus communis Forst leaves. The sensitivity test was condsucted by paper disc method. The inhibition zone of methanol extract of Artocarpus communis Forst leaves was founded as a colourless zone that obtained after incubation for 24 hours. It is means P. aeruginosa and S. Aureus are sensitive to methanol extract of this plant. The inhibition zone is shown by methanol extract of Artocarpus communis Forst leaves toward bacteria coloni growth of P. aeruginosa and S. aureus that describing the linear increasing of consentration from 5% to 20%.  It is shown that Artocarpus communis Forst leaves contains the activity compounds as antibacteria, especially P. aeruginosa and S. aureus.  
Senyawa Alkoloid dari Ekstrak Aseton Daun Sambung Nyawa Gynura procumbens L. (GpL) Darminto, .; Muharram, .
bionature Vol 10, No 1 (2009)
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v10i1.261

Abstract

These research aims to isolate and identify alkaloid compound in acetone extracts from sambung nyawa leafs Gynura procumbens L. This compound obtained trough keeping the plant and isolation. The isolation consists of extraction, fractionation, purification, and identification. The identification consists of melting point test, three phase system test, and Dragendorf test. The results of the research are: 90 mg of white crystal with the range of its melting point is 134-1360 C. The crystals indicate positively with Dragendorf test. These results indicate that the compound can be classified in the alkaloid compound.   Keywords: alkaloid, acetone, Gynura procumbens L.