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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung The Journal of Experimental Life Sciences (JELS) Indonesian Journal of Cancer Chemoprevention Program Kreativitas Mahasiswa - Penelitian The Indonesian Biomedical Journal Majalah Kesehatan FKUB Jurnal Penelitian Pendidikan IPA (JPPIPA) KESANS : International Journal of Health and Science Jurnal Ilmiah Kesehatan (JIKA)
Wibi Riawan
Biochemistry And Biomolecular Laboratory, Faculty Of Medicine, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Lactobacillus plantarum IS-20506 Probiotic Restores Galectin-4 and Myosin-1a Expressions in Duodenum, Jejunum and Ileum of Lipopolysaccharide-induced Rats Reza Gunadi Ranuh; Alpha Fardah Athiyyah; Andy Darma; Wibi Riawan; Ingrid Suryanti Surono; Ferry Sandra; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1098

Abstract

BACKGROUND: Galectin-4 and Myosin-1a are important proteins for normal intestinal brush border structure and composition. Damage of these proteins by inflammation may alter digestion, absorption and barrier function. Probiotic has been widely known in maintaining gut health. However, the molecular mechanism of Lactobacillus plantarum IS-2056 probiotic in repairing intestinal brush border is not well defined. Therefore, current study was conducted by investigating the Galectin-4 and Myosin-1a expressions in a rodent model.METHODS: Male Wistar rats were induced with/without lipopolysaccharide (LPS) and treated with/without L. plantarum IS-2056 probiotic. On the seventh day, duodenum, jejunum, and ileum were collected and analyzed with western blot and immunohistochemistry for Galectin-4 and Myosin-1a expressions.RESULTS: Rats administrated with L. plantarum IS-2056 probiotic showed significant increase of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum compared to the control group (p<0.05). While in control group, Galectin-4 level tended to increase in more distal of intestinal segment and Myosin-1a level tended to decrease in more distal intestinal segment.CONCLUSION: L. plantarum IS-20506 probiotic may facilitate the repairment of damaged intestinal brush border as demonstrated by significant restoration of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum of LPS-induced rats.KEYWORDS: Lactobacillus plantarum, IS-20506, probiotic, galectin-4, myosin-1a, duodenum, jejunum, ileum
Analysis of the Histopathology, TNF-α of Microglia Cells Expression, NRG-1/erbB Oligodendrocyte, and Ki67/Apoptosis of Dentate Gyrus Rattus novergicus Brain After Acute Traumatic Brain Injury Wibi Riawan; Putri Fitri Alfiantya; Oktavia Rahayu Adianingsih; Zulkarnaen Zulkarnaen; Alif Fariz Jazmi; Sitti Ayu Hemas N.
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp20-29

Abstract

Head trauma or traumatic brain injury (TBI) gives most serious impact on the central nervous system. Several experimental models have been established to mimic different pathogenesis characteristics of TBI. The purpose of this study was to determine whether there is evidence of hystopathological lesions in the brain tissue after Marmorou TBI models. This study uses Rattus norvegicus Sprague Dawley strain. Macroscopic and microscopic observations on the brain tissue were done. Macroscopic lesions were observed in the brain. Microscopic observation was performed with Haematoxylin-Eosin (HE) staining and immunohistochemistry on the distribution of microglia cells and pyramidal cells in the cortex. Meanwhile, the distribution of NRG-1/ErbB, proliferation, and apoptosis were observed in the hippocampus. The results of macroscopic observation showed that there were wounds caused by falling loads and vasodilatation. On microscopic observation, the TBI group showed an increase in neutrophils distribution and distribution of activated microglia to produce TNF-α, and decrease in the number of cortical pyramidal cells significantly. The distribution of NRG-1 tended to decrease after exposure of TBI and had no effect on its receptor, erbB. Exposure of TBI appears to lower the activity of neuronal cells proliferation in dentate gyrus (DG) area and significantly increase the number of apoptotic cells. Marmarou model is a physiological model of TBI that spontaneously occurs following a trauma to the head, for example trauma due to an accident. This data can be used as a preliminary data of inflammation and tissue regeneration of disrupted adult brain. Therefore, this research could be used as the basis in the studies of therapeutic agents in the process of neurogenesis of brain cells.Keywords: traumatic brain injury, ERG-1/ErbB, dentate gyrus, Ki67, TNF-a, microglia
Spade Leaf Extract Phytosome Modulates Krox-20, Neuregulinـ1, Phospholipids, and Cognitive Function of Traumatic Brain Injury Model in Rats Alif Fariz Jazmi; Putri Fitri Alfiantya; Sitti Ayu Hemas Nurarifah; Erlin Aditia Purmitasari; Lilin Andreas Vitania; Wibi Riawan
Indonesian Journal of Cancer Chemoprevention Vol 6, No 3 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss3pp105-110

Abstract

Traumatic brain injury (TBI) is a disorder of the central nervous system due to head trauma. TBI can damage nerve membrane phospholipids and decrease protein synthesis of neuregulin-1 (NRG-1) because of the transcription factor Krox-20. These conditions cause the lowering in nerve re-myelination which contribute to the decline of cognitive function. In Indonesia, citicoline is a neuroprotective drug that widely used to repair and prevent further damage of the nerve cells membrane caused by trauma. Spade leaf (Centella asiatica) extract phytosome (SEP) is a model of drug delivery system which expected to enhance the therapeutic effects as neuroprotective drug. This study aims to demonstrate and compare the effectiveness from SEP and citicoline as a neuroprotective characterized by increasing the activation of Krox-20, the expression of NRG-1, the distribution of phospholipids, and the improvement of cognitive levels on TBI-induced rats. Rats were divided into 5 groups namely: control (-); control (+); treatment with SEP 90mg/kgBW; citicoline 250mg/kgBW, and SEP in combination with citicoline. Krox-20, NRG-1, and phospholipids expression were measured by immunohistochemical assay, while cognitive function were assessed with the Morris Water Maze test. According to ANOVA test results, it was showed that SEP improved the nerve cells through the activation of Krox-20, NRG-1 expression, and distribution of phospholipids significantly (p <0.05). Based on Morris Water Maze test, SEP also improved the cognitive function in TBI-induced rats. Thus, it can be concluded that spade leaf extract phytosome combined with citicoline increase a higher phospholipids distribution and give the fastest time in the cognitive tests compared with of spade leaf extract phytosome and citicoline alone.Keywords : NRG-1, Krox-20, phospholipids, phytosome, Centella asiatica.
Anticancer Effects of LBA-ST Yogurt Supernatant on HeLa Cells via Heat Shock Protein 27 Decrease In Vitro Liziyyannida Liziyyannida; Wibi Riawan
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp16-19

Abstract

Heat Shock Protein 27 (Hsp27) is overexpressed in cervical cancer as a response to stress conditions. Hsp27 overexpression increase invasion, migration, and adhesion pathways of cancer cells. The Yogurt supernatant contains Short-Chain Fatty Acids (SCFA) include acetate, lactate, and butyrate which have anticancer activity. This study aimed to investigate supernatant of LBA-ST (Lactobacillusbulgaricus-acidophilus, Streptococcusthermophillus) Yogurt can decrease the expression of Hsp27 in HeLa culture cells. The mechanism on how supernatant yogurt inhibit invasion, migration, and adhesion was studied by immunocytochemistry. The data was then collected and analyzed using One-Way ANOVA. From this study, it can be concluded that the expression of proteins that play a role in invasion, adhesion, and migration of the Hsp27 was proven to be decreased (p< 0.05 ± 0.005).Keywords: HeLa cells, yogurt supernatant, Lactobacillus bulgaricus-acidophilus, Streptococcus thermophillus, Hsp27
Pemberian Dietilnitrosamin dan Karbontetraklorida Pada Gambaran Histologi Ginjal Tikus (Rattus Norvegicus) Almas Dwi Khairana; Ricadonna Raissa; Wibi Riawan; Suharti Suharti; Hendra Susanto; Aulanni’am Aulanni’am
Jurnal Ilmiah Kesehatan (JIKA) Vol. 4 No. 1 (2022): Volume 4 Nomor 1 April 2022
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v4i1.242

Abstract

Diethylnitrosamine (DEN) is a metabolic product of various foods and beverages in which there are preservatives in the form of nitrate and nitrite. Nitrosamines are found in various products such as alcoholic beverages, processed meats, cigarettes, and cosmetic products. DEN is toxic and carcinogenic to organs such as the liver, lungs, kidneys, skin, stomach, and blood. In addition to giving DEN compounds, there are also CCl4 compounds as tumor promoter agents known as genotoxic substances that can increase the risk of genetic errors and stimulate cells to become malignant neoplasms. Administration of DEN and CCl4 induction can damage various organs of the body, one of which is the kidney. In a healthy body, angiogenesis plays a role in wound healing and the formation of new tissue. However, angiogenesis also contributes to carcinogenesis or the uncontrolled growth of cancer cells, and is malignant when the body is contaminated with toxic substances. Therefore, to determine the effect of the administration of DEN and CCl4 observations were made using the immunohistochemical method, to see differences in the histology of the kidneys of experimental animals, the positive control group, and the negative control group based on the VEGF angiogenesis factor. From these results, there are significant differences. In the negative group, there was no necrosis and high-value VEGF expression, but in the positive group, there was necrosis and a decrease in the value of VEGF expression compared to the negative control group.
Effectiveness of Extract Supplements (Apium graveolens L.) to Estrogen Receptors Expression and Oocyte Diameter at Nucleolar Chromatin and Perinucleolar Stages in Female of Nile tilapia (Oreochromis niloticus L.) Rikardo, Novy Kurnia; Marhendra, Agung Pramana Warih; Kurniawan, Nia; Riawan, Wibi; Rahayu, Sri
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.03

Abstract

The study of the use of celery (Apium graveolens L.) in the health sector has been widely discussed. The analysis of the use of celery in the process of oocyte development still needs to be addressed. This study was conducted to see the effect of celery extract on the expression of estrogen receptors on the gonads and oocyte diameter in tilapia for the first time. Fish are divided into four groups: P0 = Control treatment, feed without mixture; P1 = Treatment with celery extract concentration of 2,000 mg.kg-1 of feed; P2 = Treatment with celery extract concentration of 4,000 mg.kg-1 of feed; P3 = Treatment with celery extract concentration of 6,000 mg.kg-1 of feed. The results showed celery extracts expressed estrogen receptors on the gonads. The expressed estrogen receptor is the estrogen receptor β, whereas the estrogen receptor α is not detected in the gonad. Group statistical tests ANOVA showed no significant results in the development of oocytes. On the other hand, celery extract also impacted the development of oocytes at the nucleolar stage of chromatin, with a markedly greater oocyte diameter. Meanwhile, in the perinucleolar stage, celery extract reduces the oocyte diameter. Keywords: Apium graveolens L, ERα, ERβ, nucleolar chromatin, perinucleolar.
Modulation of Endothelin-1 and Placental Microvascular Diameter by Extra Virgin Olive Oil in Preeclampsia Rat Model Dian Lovita, Agnestia Naning; Silvani, Yulia; Maharani, Afniari; Riawan, Wibi
Jurnal KESANS : Kesehatan dan Sains Vol 4 No 11 (2025): KESANS: International Journal of Health and Science
Publisher : Rifa'Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54543/kesans.v4i11.422

Abstract

Introduction: Preeclampsia is linked to abnormal placental vasoconstriction and elevated endothelin-1 (ET-1). Polyphenols in Extra Virgin Olive Oil (EVOO) has antioxidant and vasoprotective properties, but its impact on ET-1 and placental microvasculature in preeclampsia is unclear. Objective: Investigate whether EVOO can attenuate hypertension and improve placental vascular health in preeclampsia. Method: 20 pregnant rats randomly assigned to five groups: normal (K−), preeclampsia model (K+), and three EVOO-treated groups (D1, D2, D3) receiving 0.5, 1, and 2 mL/day, respectively. Preeclampsia was induced with L-NAME, blood pressure was monitored on gestational days 12, 15, and 19. Placental ET-1 expression and microvessel diameter were assessed on day 19 using immunohistochemistry. Result and Discussion: EVOO treatment normalized blood pressure by gestational day 19. ET-1 expression increased with EVOO dose suggesting a role in vascular remodelling. Microvessel diameter improved in D1 and D2 but slightly decreased in D3. Conclusions: EVOO mitigates hypertension and enhances placental microvasculature in preeclampsia, with optimal effects at moderate doses.
Oxyhydrogen Nanobubbles Suppress FoxP3 and Ki-67 Expression in a Wistar Rat Model of Hepatocellular Carcinoma Zahrah, Natasya Adiba; Widyarti, Sri; Wuragil, Dyah Kinasih; Riawan, Wibi; Indrajani, Olly; Lubab, Ahmad; Hernowo, Aditya Tri; Sumitro, Sutiman Bambang; Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13858

Abstract

HCC is difficult to treat due to its complex tumor microenvironment and the ineffectiveness of available therapies. Gas-based anticancer approaches are promising, but are hampered by instability and difficulties in targeted delivery. HHOnbs (containing hydrogen (H2), oxygen (O2), and low-dose hydrogen peroxide (H2O2) at the nanoscale) have been proposed to improve the stability and precision of gas-based therapies. This study aimed to investigate the effects of HHOnbs on the immunoregulatory marker FoxP3 and the proliferation marker Ki-67 in a Wistar rat model of HCC induced by diethylnitrosamine (DEN)/carbon tetrachloride (CCl4). The design used was a true experimental design. HHOnbs were administered intravenously. Liver tissue was assessed using immunohistochemistry to measure FoxP3 and Ki-67 expression. HHOnbs treatment significantly reduced FoxP3 and Ki-67 expression (p<0.05). FoxP3 is a marker of regulatory T cells (Tregs), which often inhibit antitumor immune responses. Its decrease indicates an increased immune response potential. Ki-67 is a marker of cell proliferation. Its decrease indicates the suppression of cancer cell growth. Further investigations are warranted to compare its efficacy with standard therapies, assess different treatment durations, and elucidate the underlying molecular signaling pathways.
Co-Authors Agung Pramana Warih Marhendra, Agung Pramana Warih Ahmad Lubab, Ahmad Alif Fariz Jazmi Alif Fariz Jazmi Almas Dwi Khairana Alpha Fardah Athiyyah Andy Darma Aris Widayati Asnah Hidayat Aulanni'am Aulanni'am, Aulanni'am Desie Suci Permata Sari, Desie Suci Edy Widjajanto Erita Rahmani, Erita Erlin Aditia Purmitasari Ferry Sandra Fitri Armania Hendra Susanto HERAWATI, LILIK Hernowo, Aditya Tri HMS. Chandra Kusuma I Gusti Made Reza Gunadi Ranuh I Ketut Gede Muliartha Indrajani, Olly Ingrid S. Surono Ingrid Suryanti Surono Kusworini Handono Kalim Laksmi Wulandari Lilin Andreas Vitania Liziyyannida Liziyyannida Lizziyyannida Lizziyyannida, Lizziyyannida Lovita, Agnestia Naning Dian M. R. Indra Maharani, Afniari Masdar Muid Muslim Akmal Nancy M. Rehatta, Nancy M. Nia Kurniawan Nunuk Sri Muktiati Oktavia Rahayu Adianingsih Putri Fitri Alfiantya Putri Fitri Alfiantya Raden Argarini Ricadonna Raissa Rikardo, Novy Kurnia Samodijanti Wibowati Samodrijanti Wibowati Satya Hanggara Putra Pratama, Satya Hanggara Silvani, Yulia Sitti Ayu Hemas N. Sitti Ayu Hemas Nurarifah SRI RAHAYU Sri Widyarti Sudarmo, Subijanto Marto Suharti Sutiman B. Sumitro Titis Sari Kusuma Tri Yudani Mardining Raras Triwahju Astuti Wigati, Kristanti Wanito Wildan Mochamad Ridho, Wildan Mochamad Wuragil, Dyah Kinasih Zahrah, Natasya Adiba Zulkarnaen Zulkarnaen Zurniatus Sholihah, Zurniatus