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All Journal bionature Science and Technology Indonesia Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi Window of Health : Jurnal Kesehatan PREPOTIF : Jurnal Kesehatan Masyarakat Pharmaceutical Reports Journal Microbiology Science Jurnal Novem Medika Farmasi MPSJ

Tadjuddin Naid

Laboratorium Kimia Farmasi, Fakultas Farmasi, Universitas Hasanuddin, Makassar

Author-ID : 327678

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Veterinary

Published : 30 Documents Claim Missing Document

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Articles

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IDENTIFICATION AND ANTIBACTERIAL ACTIVITY TEST OF ENDOPHYTE FUNGI MAHONI BARK (Swieenia mahagoni L.) IFKBM04 Nuryanti, Siska; Rosdah, Rosdah; Naid, Tadjuddin; Hardiyanti, Vivi
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Endophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites. This study investigated the antibacterial activity of endophytes fungi IFKBM04 isolated from Swietenia mahagoni L. and identified macroscopic and microscopic characteristics of endophytic fungi. Based on the results of macroscopic and microscopic identification compared with the literature to determine the general characteristics of the sample. The results of this morphological identification showed that the sample of mahogany bark isolate (Swietenia mahagoni L.) code IFKMB-04 had similarities with the genus Saccharomyces sp.

Evaluation of Ethanol Extract Tapak Dara Leaf (Catharanthus roseus L. ) for Antibacterial Activity against Skin Pathogens Alhijrah, Yayu; Naid, Tadjuddin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1006

Tapak dara leaves (Catharanthus roseus L.) are known to be efficacious for relieving muscle pain, antidepressants, as well as medicine for various diseases such as relieving swelling from wasp stings, nosebleeds, and sore throats, antidotes, antibacterials, and lowering blood pressure in humans because they contain chemical compounds such as essential oils, phenolic acids, flavonoids, tannins, and alkaloids. This study aims to determine the antibacterial activity of the ethanol extract of tapak dara leaves against bacteria that cause skin infections. This type of research is done in an experimental laboratory using the agar diffusion method, which is based on inhibiting the growth of bacteria that cause skin infections. The stages of this research began with sample preparation, screening for antibacterial extract activity, and testing the antibacterial extract activity at several concentrations against the test bacteria that cause skin infections. This study obtained the results that the ethanol extract of tapak dara leaves tested positive for inhibition of the growth of Staphyloccus aureus, Staphylococcus epidermidis, Pseudomonas aeroginosa, and Propionibacterium acne based on the extract screening results. The results of this study also showed that the ethanol extract of tapak dara leaves at a concentration of 40% had the greatest inhibition using the agar diffusion method based on the diameter of the inhibition zone, with a value of 8.37 mm (Propionibacterium acne); 8.47 mm (Pseudomonas aeruginosa); 9.19 mm (Staphylococcus epidermidis); and 11.68 mm (Staphylococcus aureus)

IDENTIFIKASI KOMPONEN KIMIA EKSTRAK ETANOL DAUN PACAR KUKU (Lawsonia inermis L.) SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN Herwin, Herwin; Nurung, Ayyub Harly; Ambon, Nur Intan; Naid, Tadjuddin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Prevention of the transmission of covid 19 can be done by implementing the health protocol, namely 3M (wearing masks, washing hands and social distance). Based on research, the use of masks can reduce the risk of transmission of COVID-19 by 45 percent as well as hand washing reduces the risk by 35 percent. WHO recommends washing hands with soap/antiseptic for 20-30 seconds and applying the right steps. Another effort is to maintain a distance of at least 1 meter and avoid crowds. To assist the government in preventing the transmission of covid 19, socialization of the 3M activity was carried out at the Darul Istiqamah Maros Islamic Boarding School which is located on the Maros-Makassar Street, Mandai District, Maros Regency. This location was chosen because Maros was the third highest area with the number of people who are positive for the corona virus in South Sulawesi. Islamic boarding school students are a partner group that is expected to apply the 3M health protocol so as to prevent the transmission of covid 19. The activity is carried out by providing counseling about the importance of implementing 3M, how to implement 3M and also by distributing masks, soap and disinfectants needed to implement health protocols. The activity was carried out well and received a good response from the participants with 33 participants from various classes who were students of the Darul Istiqamah Islamic Boarding School Makassar.

Antibacterial Activity Test of Ceremai Leaf Ethanol Extract (Phyllanthus acidus (L.) Skeels) Against Bacteria Causing Skin Infections by KLT-Bioautography and Agar Diffusion Naid, Tadjuddin; Herwin, Herwin; Muldin, Nurul Insani
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1204

Ceremai leaves (Phyllanthus acidus) are known for their medicinal properties, including the treatment of infectious diseases. This study aims to evaluate the antibacterial activity of ethanol extract from Ceremai leaves against skin infection-causing bacteria using Thin Layer Chromatography-Bioautography (TLC-Bioautography) and agar diffusion methods. The study began with an antibacterial screening test, followed by TLC-Bioautography, and then determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) at various concentrations (0.125% to 16%). The agar diffusion method was used to further assess antibacterial activity. Screening results showed antibacterial activity at a concentration of 1%. TLC-Bioautography with a chloroform: methanol: water (20:2:0.5) eluent revealed active spots with Rf values of 0.8, 0.54, 0.36, and 0.29. The MIC values were 0.25% for P. acnes and S. aureus, and 0.5% for P. aeruginosa and S. epidermidis. The MBC values were 0.5% for P. acnes and S. aureus, and 1% for P. aeruginosa and S. epidermidis. The largest inhibition zone in the agar diffusion test was observed at a 32% concentration against S. aureus, measuring 20.11 mm. These findings suggest that Ceremai leaf extract has significant antibacterial potential and could be considered for developing alternative treatments for skin infections.

Antimicrobial Activity of Ethanol Extract of Pinang Seed (Areca catechu L.) Against the Growth of Candida albicans and Malassezia furfur Fungi Using the Agar Diffusion Method Naid, Tadjuddin; Saputri, Febrianti Ayu; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1229

Pinang seeds (Areca catechu L.) are known for their various benefits, including antimicrobial properties. This study aims to evaluate the antimicrobial activity of ethanol extract from pinang seed against the growth of Candida albicans and Malassezia furfur fungi using the agar diffusion method. The research involved extracting the pinang seed using maceration with 96% ethanol, followed by evaporation using a rotary vacuum evaporator to obtain a concentrated extract. Antimicrobial activity was tested across concentrations of 32%, 16%, 8%, 4%, 2%, 1%, 0.5%, and 0.1%. The results showed that at a concentration of 32%, the ethanol extract produced the largest inhibition zones: 27.78 mm against Candida albicans and 15.51 mm against Malassezia furfur, both categorized as very strong activity. These findings suggest that ethanol extract of pinang seed has significant potential as an antimicrobial agent, particularly against fungal pathogens.

Detection of Salmonella Bacteria In Soybean Tempeh Produced In Palekko Village, Takalar Regency Anggraeni, Nur Afni; Naid, Tadjuddin; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1231

Tempeh is a staple food in Indonesia, known for its nutritional benefits, including 25% protein, 5% fat, 4% carbohydrates, vitamin B12, and rich mineral content. Ensuring the safety and hygiene of tempeh is crucial, especially in preventing contamination by harmful bacteria like Salmonella, which poses serious health risks. This study aimed to detect the presence of Salmonella bacteria in tempeh produced in Palekko Village, Takalar Regency, using Salmonella Shigella Agar (SSA) medium. The method employed was experimental, involving the preparation and dilution of tempeh samples followed by incubation on SSA medium. The results is a no samples detected Salmonella met the Salmonella contamination limit requirements, which were negative/25g.

Antibacterial Activity of N-Hexane and Ethyl Acetate Fractions Of Pandanus Nut (P. Julianettii) Against Oral Pathogens Nur, Wardah Rafidah; Naid, Tadjuddin; Herwin, Herwin
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1333

Oral infections caused by Streptococcus mutans and Porphyromonas gingivalis are common health problems. This in vitro study aimed to evaluate the antibacterial activity of Pandanus nut (Pandanus julianettii) with n-hexane and ethyl acetate fractions against these pathogens through agar diffusion assays. The n-hexane and ethyl acetate fractions were subjected to agar diffusion testing, with Minimum Inhibitory Concentration (MIC) values determined at 0.8% and 0.2% respectively against both bacterial strains. Subsequent Minimum Bactericidal Concentration (MBC) testing revealed values of 6.4-12.8% for n-hexane fraction and 3.2-6.4% for ethyl acetate fraction. In agar diffusion assays, the n-hexane fraction at 12.8% concentration produced inhibition zones of 15.54±0,20 mm on S. mutans and 15.46±0,35 mm on P. gingivalis. The ethyl acetate fraction showed significantly larger inhibition zones of 18.76±0,79 mm against S. mutans and 15.59±0,42 mm against P. gingivalis at the same concentration. These findings demonstrate that both n-hexane and ethyl acetate fractions of Pandanus nut possess substantial antibacterial activity against oral pathogens.

Antibacterial Activity of Ethanolic Extract of Pandanus Nut (Pandanus julianettii) Gastrointestinal Bacteria Using The Agar Diffusion Method Monika, Arum; Naid, Tadjuddin; Herwin, Herwin
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1334

Gastrointestinal infections are a major global health concern, often caused by pathogenic bacteria. This study aimed to evaluate the antibacterial activity of the ethanolic extract of Pandanus nut (Pandanus julianettii) against gastrointestinal pathogens using the agar diffusion method. The Minimum Inhibitory Concentration (MIC) of the extract was determined to be 6.4% for Shigella dysenteriae, Salmonella typhi, and Escherichia coli, and 3.2% for Vibrio cholerae. The Minimum Bactericidal Concentration (MBC) for all tested bacteria was 12.8%. Antibacterial activity analysis revealed that at the 12.8% concentration, the largest inhibition zones were observed: 22.68 mm for Salmonella typhi, 22.20 mm for Shigella dysenteriae, 21.26 mm for Escherichia coli, and 20.80 mm for Vibrio cholerae. These findings indicate that the ethanolic extract of Pandanus julianettii possesses strong antibacterial properties and holds potential as a natural therapeutic agent for managing gastrointestinal infections.

Co-Authors A Amaliah Dahlia ABD. MALIK Adelina, Nabila Aisya, A. Henni Alhijrah, Yayu Ambon, Nur Intan Aminah Aminah Andi Maulana Kamri Anggraeni, Nur Afni Anisa, Rianti Asni Amin Bantulu, Ananta Rahayu Dewi Yuliana Dharmawat, Dewi Tri Fitriana Fitriana Fitriani Mangerangi, Fitriani Gemini Alam Hanifah Almahdaly, Hanifah Hardiyanti, Vivi Hasan, Tahirah Herwin Herwin, Herwin Irfanitha, irfanitha Kalsum T, Ummu Kasim, Athia Kurnia Kus Haryono Latif, Safira Kartika Handayani Mamat Pratama, Mamat Marda, Marda Mas'ud, Ita Ayuningsih Maulidiyah, Zakiya Mirawati Mirawati Monika, Arum Muhammad Aswad Muldin, Nurul Insani Muzakkir Baits, Muzakkir Nasution, Annio Indah Lestari Nisaa, Nur Rezky Khairun Nur, Wardah Rafidah Nurung, Ayyub Harly Nuryadin, Yudhi Nuryadin, Yudhi Paduai, Muh. Harry Bagas Setiawan R. Palongko, Siska Oktavina S. Qawiy, Haqi Romadhona R. Rusli Rahmawati, Rahmawati Ramadhani, Nur Ramadini, Ristieyen Ramlan, Pratiwi Risfah Yulianty Rosdah, Rosdah Sadjidin, Sugiarto Saputri, Febrianti Ayu Seniwati Dali Seniwati Seniwati, Seniwati Septiyanti, Septiyanti Siska Nuryanti, Siska Umasangaji, Ria Fitriani Virsa Handayani Wahab, Ummu Kalsum Yenni Triana, Yenni Yoga Yuniadi Yusnita Rifai, Yusnita Zainal Abidin

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