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1 Pengaruh Biji EKstrak Barringtonia asiatica L. (Kurz) (Lecythidaceae) Terhadap Mortalitas Larva dan Fekunditas Crocidolomia pavonana F. (Lepidoptera: Pyralidae) Danar Dono; Syarif Hidayat; Ceppy Nasahi; Emelda Anggraini
Agrikultura Vol 19, No 1 (2008): April, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2528.08 KB) | DOI: 10.24198/agrikultura.v19i1.601

Abstract

Ekstrak biji Barringtonia asiatica (Lecythidaceae) memiliki aktivitas insektisida, namun pengaruhnya terhadap fekunditas Crocidolomia pavonana (Lepidoptera: Pyralidae) belum diketahui. Percobaan uji toksisitas dilakukan urituk mendapatkan nilai LC50 dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri aras perlakuan ekstrak biji B. asiatica pada konsentrasi 0.02%: 0.05%: 0,1%: 0.2%; 0.3%: dan kontrol. Masing-masing perlakuan diulang tiga kali. Pengujian dilakultan dengan menggunakan metode residu pada daun sawi pakan. Uji pengaruh ekstrak biji B. asiatica terhadap fekunditas C. pavonana dilakukan dengan metode residu pada daun sawi pakan ke dalam ekstrak biji B asiatica pada konsentrasi 0.09%; 0,15%; 0,22% - (setara dengan LC30, LC50, LC70); dan kontrol. Imago yang berkembang dari larva yang diberi pakan perlakuan diamati fekunditasnya. Hasil percobaan menunjukkan bahwa ekstrak biji B. asiatica bersifat toksik terhadap larva C. pavonana dengan nilai LC50 sebesar 0,15% dan memiliki pengaruh sebagai penghambat aktivitas makan (antifidan). Ekstrak biji B. asiatica pada selang konsentrasi 0,09%-0.22% yang diberikan pada larva C. pavonana berpengaruh terhadap waktu pembentukkan telur, produksi telur, masa oviposisi, dan fertilitas dibandingkan dengan perlakuan kontrol.
PerkecambahanPeronosclerospora spp. Asal Beberapa Daerah di Jawa Barat pada Fungisida Berbahan Aktif Metalaksil, Dimetomorf dan Fenamidon Fitri Widiantini; Dwisari Januarily Pitaloka; Ceppy Nasahi; Endah Yulia
Agrikultura Vol 28, No 2 (2017): Agustus, 2017
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (733.947 KB) | DOI: 10.24198/agrikultura.v28i2.15753

Abstract

ABSTRACTGermination of Peronosclerospora spp. isolated from several maize plantation areas in West Java on fungicides containing active ingredient of metalaxyl, dimetomorph and fenamidoneDowny mildew is a major disease in corn plants that affect the world corn production, include in Indonesia. The control of downy mildew that caused by pathogen Peronosclerospora spp. are relying on the use of synthetic fungicides. However, the emergence of the Peronosclerospora spp. resistant against fungicides rise awareness for the need of constant surveillance. The aim of this study was to know the effectiveness of three commonly used active ingredients fungicide (Metalaxyl, Dimetomorph, and Fenamidone) in controlling Peronosclerospora spp. from five regions in Wes Java (Sumedang District,Bandung District, Majalengka District, Garut District and West Bandung District). Experiment was conducted using conidia germination method. The results of this study demonstrated that fungicide with active ingredient of Dimethomorph was able to suppress the germination of conidia Peronosclerospora spp. with 0% of conidia germination in 4 districts (Sumedang, Bandung, Majalengka and Garut) and only 0.47% conidia germinated in isolate collected from West Bandung District. Meanwhile, fenamidon experienced a decrease in effectiveness at West Bandung District. In this study, it was also found that the number of conidia germinated on isolates treated with Metalaxyl did not show any significant difference compared to control. This suggested that Peronosclerospora spp. might resistant to Metalaxyl.Keywords: Downy mildew, Peronosclerospora spp., metalaxyl, dimetomorph, fenamidoneABSTRAKPenyakit bulai merupakan penyakit utama pada tanaman jagung yang berpengaruh terhadap produksi jagung dunia, termasuk di Indonesia. Pengendalian penyakit bulai yang disebabkan oleh patogen Peronosclerospora spp. masih bertumpu pada penggunaan fungisida sintetik. Akan tetapi, terjadinya penurunan keefektifan fungisida terhadap Peronosclerospora spp. menyebbkan diperlukannya monitoring secara berkala. Penelitian ini bertujuan untuk mengetahui keefektifan tiga jenis bahan aktif fungisida yang umum digunakan (Metalaksil, Dimetomorf, dan Fenamidon) dalam mengendalikan penyakit bulai terhadap patogen Peronosclerospora spp.asal lima daerah di Jawa Barat (Kab. Sumedang, Kab. Bandung, Kab. Majalengka, Kab. Garut dan Kab. Bandung Barat). Pengujian keefektifan fungisida dilakukan dengan menggunakan metode perkecambahan konidia. Hasil penelitian menunjukkan bahwa perlakuan fungsida dimetomorf terhadap isolat Peronosclerospora spp. asal semua daerah dapat menekan perkecambahan konidia dengan persentase perkecambahannya 0% pada 4 daerah dan 0,47% pada isolat asal daerah Kab. Bandung Barat. Sementara Fenamidon menujukkan indikasi penurunan keefektifan di daerah Bandung Barat. Pada penelitian ini, ditemukan pula adanya indikasi resistensi patogen Peronosclerospora spp. terhadap fungisida Metalaksil. Hal ini terlihat dari perkecambahan konidia yang diperlakukan dengan Metalaksil yang hasilnya tidak berbeda nyata dengan kontrol yang tidak diperlakukan dengan fungisida.Kata Kunci: Penyakit bulai jagung, Peronosclerospora spp., Metalaksil, Dimetomorf, Fenamidon
PENGUJIAN KEMAMPUAN BEBERAPA BAHAN KIMIA DAN AIR PERASAN DAUN TUMBUHAN DALAM MENGINDUKSI RESISTENSI TANAMAN PADI TERHADAP PENYAKIT BERCAK DAUN CERCOSPORA Tarkus Suganda; Endah Rismawati; Endah Yulia; Ceppy Nasahi
Bionatura Vol 4, No 1 (2002): Bionatura Maret 2002
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Beberapa bahan kimia (asam salisilat, kitin asal kulit udang dan K2HPO4) dan airperasan daun tumbuhan (daun bayam, daun beluntas, dan daun melati) telahdiuji coba di rumah kaca untuk menginduksi resistensi tanaman padi cv. IR-64terhadap penyakit bercak daun cercospora. Benzothizadiazole-mankozeb(Bion), suatu bahan penginduksi (plant activator) komersil digunakan sebagaipembanding. Percobaan dilakukan di rumah kaca Jurusan Ilmu Hama danPenyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran di Jatinangor (700m d.p.l.) dari bulan April s.d. Oktober 2001, dengan menggunakan RancanganAcak Lengkap dan empat ulangan. Perlakuan penginduksian dilakukan dua kalidengan selang waktu seminggu. Penginokulasian dengan jamur Cercosporaoryzae dilakukan seminggu setelah perlakuan terakhir, sehingga tidak terjadikontak antara perlakuan dengan jamur patogen. Hasil percobaan menunjukkanbahwa asam salisilat, kitin asal kulit udang, dan K2HPO4 mampu menginduksiresistensi tanaman padi IR-64 sebagaimana diperlihatkan oleh intensitasserangan penyakit bercak daun cercospora yang tidak berbeda nyatadibandingkan dengan perlakuan pembanding. Air perasan daun tumbuhan yangdiuji, tidak memperlihatkan hasil yang memuaskan dan intensitas seranganpenyakitnya tidak berbeda dengan perlakuan kontrol (inokulasi patogen tanpaperlakuan penginduksi).Kata kunci : Resistensi sistemik terinduksi, bercak daun cercospora, padi
DETEKSI JAMUR Verticillium dahliae KLEBHAN PENYEBAB PENYAKIT LAYU TANAMAN DI SENTRA BUDIDAYA KENTANG LEMBANG DAN PANGALENGAN Tarkus Suganda; Fitri Widiantini; Andang Purnama; Ceppy Nasahi
Bionatura Vol 5, No 1 (2003): Bionatura Maret 2003
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Jamur V. dahliae merupakan jamur patogenik yang sangat merugikan berbagaijenis tanaman hortikultura, termasuk kentang, tomat, dan cabai. Sekali jamur initerinfestasikan ke suatu daerah pertanaman, maka ia dapat menyebabkanpenyakit yang hebat dan kerugian hasil. Kultivar resisten belum tersedia padatanaman apa pun, dan jamur ini mampu bertahan di dalam tanah untuk waktuyang lama serta memiliki kisaran inang yang luas. Suatu survey untukmengetahui keberadaan jamur V. dahliae di sentra pertanaman hortikultura diKecamatan Lembang dan Pangalengan telah dilakukan. Sampel tanah diambildari sembilan lokasi di tiga desa di Kecamatan Lembang dan dari 48 lokasi di limadesa di Kecamatan Pangalengan. Pengisolasian dilakukan dengan metodepengenceran dan penebaran suspensi ke atas media PDA dan ESA yang dilakukandi Laboratorium Fitopatologi Jurusan Ilmu Hama dan Penyakit Tumbuhan,Fakultas Pertanian Universitas Padjadjaran di Jatinangor. Penyampelan danpengisolasian dilakukan dua kali. Namun demikian, dari semua sampel, tidakditemukan keberadaan jamur V. dahliae dari Lembang dan Pangalengan.Kata kunci : Verticillium dahliae, deteksi, kentang, Lembang, Pangalengan
SOSIALISASI DAN PELATIHAN PEMBUATAN KASCING SERTA PEMANFAATANNYA UNTUK PENGENDALIAN PENYAKIT TANAMAN DI DESA PAGERWANGI, LEMBANG, BANDUNG BARAT Noor Istifadah; Ceppy Nasahi; Fitri Widiantini; Sri Hartati
Dharmakarya Vol 9, No 2 (2020): Juni, 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v9i2.25479

Abstract

Sebagian besar masyarakat Desa Pagerwangi, Lembang, Bandung Barat adalah petani dan peternak terutama ternak sapi. Salah satu kendala utama yang dihadapi petani dalam budidaya tanamannya adalah adanya penyakit. Penggunaan pestisida yang terus menerus dapat menimbulkan berbagai dampak negatif. Salah satu cara pengendalian yang ramah lingkungan adalah dengan menggunakan bahan organik. Namun demikian banyak petani yang belum tahu mengenai hal tersebut. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk menyosialisasi dampak negatif dari pestisida dan perlunya pengendalian secara ramah lingkungan terutama dengan pemanfaatan bahan organik. Program yang telah dilaksanakan adalah penyuluhan tentang pentingnya pengendalian secara ramah lingkungan dan pemanfaatan bahan organik untuk pengendalian penyakit untuk petani di RW 10. Kegiatan ini ditindaklanjuti dengan pemberian benih sayuran dan pengarahan pemanfaatan bahan organik untuk pembuatan pupuk organik cair dan untuk pengendalian penyakit tanaman. Guna mendukung produksi bahan organik, dilakukan juga program yang berupa penyuluhan tentang pemanfaatan kotoran sapi untuk budidaya cacing yang limbahnya yaitu berupa kascing merupakan bahan organik yang sangat baik untuk pupuk dan juga pengendali penyakit tanaman. Penyuluhan ini ditindaklanjuti dengan adanya pelatihan budidaya cacing dan pembuatan kascing yang diikuti peternak, petani, serta wanita tani dari RW 03 dan RW 10. Selain diberikan keterampilan, peserta juga diberikan media dan bibit cacing, serta kotak kayu serta unit percontohan untuk budidaya cacing. Pendampingan dan koordinasi juga dilakukan untuk memfasilitasi pelaksanaan budidaya cacing, produksi kascing serta pemanfaatannya dalam budidaya tanaman ramah lingkungan.
Potency of Yeasts to Promote Induced Resistance on Chili Plant (Capsicum annuum L.) against Yellow Leaf Curl Disease Sri Hartati; Rika Meliansyah; Luciana Djaya; Ceppy Nasahi; Ramadhan Kamal Putra
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.52089

Abstract

Yellow leaf curl is a major disease on chili plants. The use of antagonistic yeasts as the control agents is a good alternative in  an environmentally friendly control method. This study was objected to evaluate the potencies of Rhodotorula minuta and Candida tropicalis to promote induced resistance on chili plants against yellow leaf curl disease. The experiment was arranged in the randomized complete block design with 9 treatments and 3 replications. The treatments were the application of the yeasts at three different times of virus inoculation, as follows: A. R. minuta, virus inoculation at 3 days after transplanting (dat), B. R. minuta, virus inoculation at 7 dat, C. R. minuta, virus inoculation at 10 dat, D. C. tropicalis, virus inoculation at 3 dat, E. C. tropicalis, virus inoculation at 7 dat, F. C. tropicalis, virus inoculation at 10 dat, G. control, virus inoculation at 3 dat, H. control, virus inoculation at 7 dat, and I. control, virus inoculation at 10 dat. No treatment was applied to the control.  The yeasts were applied by soaking the chili seeds, and pouring the suspension into the growth media at transplanting. The variables observed were incubation period, disease severity, and disease incidence. Peroxidase activity, phenylalanine ammonia lyase activity, and salicylic acid accumulation were also analyzed. The results showed that the application of R. minuta or C. tropicalis was able to extend the disease incubation period, but did not reduce the yellow leaf curl disease incidence and severity. The treatment of R. minuta, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6870.93 units (0.5 minute) and from 577.367 units to 1131.300 units (2.5 minutes), PAL activity from 16.059 to 17.911 A290/mg, and accumulation of salicylic acid from 2.785 to 6.263 ppm. Application of C. tropicalis, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6033.067 units (0.5 minute) and from 577.367 units to 950.967 units (2.5 minutes), and accumulation of salicylic acid from 2.785 to 6.982 ppm.
The effect of Tofu Wastewater on Cabbage Growth and Preference of Plutella xylostella Siska Rasiska; Nasya Nabila; Danar Dono; Toto Sunarto; Ceppy Nasahi
CROPSAVER Vol 5, No 1 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i1.36927

Abstract

Tofu wastewater containing a high proportion of organic compound may cause various adverse impacts, such as water pollution which bad smell and degrading the aesthetics of the environment.  The efforts uses of tofu wastewater is a organic liquid fertilizer. The aims of this study was to determine the effect of tofu wastewater on cabbage growth and preference of Plutella xylostella. The experiment was carried out from April to June 2018, at Ciparanje Experimental Field, Faculty of Agriculture, Universitas Padjadjaran. Randomized block design was used with nine treatment concentration of tofu wastewater consist of control 0%, 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, 100%, and three replication. The result showed that tofu wastewater by 100% could increase the growth of cabbage. The highest female P. xylostella preference.
Efek Pencampuran Bahan Pestisida Nabati Terhadap Keefektifannya Dalam Menekan Colletotrichum sp. In Vitro Serta Penyakit Antraknosa Pada Stroberi Noor Istifadah; Anggi Ayuningtyas; Ceppy Nasahi
Agrologia Vol 6, No 1 (2017): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i1.177

Abstract

Antrachnose disease caused by Colletotrichum sp. is one of the important diseases of strawberry in the field or after postharvest. The environmentally-friendly control measures is the use of botanical pesticides. Botanical pesticides that are commonly used by the farmers are mixture of several kinds of plant extract. This paper discuses the effects of combining water extract of garlic, galangal rhizome, and betel leaves on their efficacy in inhibiting conidial germination of Colletotrichum sp., the growth of miselium in vitro and the development of antracnose disease in strawberry. Preliminary study was conducted to determine the concentration of the extract that was used for further experiment. The experiment was arranged in the completely randomized design with 10 treatments involving water extract of garlic, galangal rhizome, betel leaves and their mixtures, fungicide and control/check. Each treatment was repeated three times.The results showed that combination of water extract of garlic, galangal rhizome and betel leaves did not enhance their efficacy. The inhibition effect of garlic water extract (5% concentration) was relatively better than its mixtures with galangal and betel leaves (total concentration 15%). The effects of galangal rhizome or betel leaves water extract and their mixtures were not significantly different. Water extract of garlic (15 %) inhibited Colletotrichum sp. which was showed by inhibition zone of 9.3 mm width; and suppressed the antrachnose disease in strawberry stolon by 83,7 %. 
Antagonistic Effects of Bacterial Rhizosphere of Oil Palm in Biocontrol of Basal Stem Rot Disease (Ganoderma boninense Pat.) Widiantini, Fitri; Nugraha, Gema Takbir; Yulia, Endah; Nasahi, Ceppy
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1071-1081

Abstract

Basal stem rot disease caused by Ganoderma boninense is a major problem for oil palm cultivation. The research was conducted to obtain biocontrol agents from rhizosphere of oil palm to control the disease as part of sustainable pest management in oil palm plantation. Rhizosphere bacteria were isolated from rhizospheres of healthy oil palm trees. Isolation of bacteria was done using serial dilution method. The isolated bacteria were then tested for its antifungal activity against G. boninense in vitro using dual culture assay. The ability of the bacteria to produce antifungal compound was also determined by culturing the bacteria on ISP2 liquid media. Once the bacterial cells were removed, the crude metabolites were then tested against G. boninense using agar well diffusion and toothpick colonization. The result showed that several isolates demonstrated strong antifungal activity against G. boninense. Some isolates were also able to degrade chitin and to solubilize phosphate. Furthermore, the crude metabolites produced by the rhizosphere bacteria demonstrated the ability to inhibit the growth of G. boninense in the agar diffusion method. Colonization of the G. boninense on toothpick following soaking in the crude metabolites was also inhibited. The isolated rhizosphere bacteria (BARK7 and BARK15 in which identified as Burkholderia sp.) showed promising ability to be developed as biocontrol agent for basal stem rot disease of oil palm.
Inhibition of the In Vitro Growth of Colletotrichum sp. the Cause of Anthracnose on Avocado Fruit by Yeast Hartati, Sri; Rahmah, Syifa Aulia; Nasahi, Ceppy
CROPSAVER Vol 8, No 1 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i1.63318

Abstract

Anthracnose caused by Colletotrichum sp. is a major disease on  avocado fruit. An alternative to control this post harvest disease is by using biocontrol agents, such as yeasts. The research was objected to test the abilities of three yeast isolates i.e. Aureobasidium pullulans Dmg 11 DEP, Rhodotorulla minuta Dmg 16 BE and Candida tropicalis Lm 13 BE, to inhibit the in vitro growth of Colletotrichum sp. The experiment was arranged in the completely randomized design consisting of five treatments that were repeated four times each. The treatments were dual culture and double dish system of the culture of Colletotrichum sp. vs the yeast isolates as follow A. pullulans Dmg 11 DEP, R. minuta Dmg 16 BE, C. tropicalis Lm 13 BE, fungicide mancozeb, and a control. The results showed that the three yeast isolates were able to inhibit the colony growth of Colletotrichum sp. by 19,9% – 56,10%  on the dual culture and 15,56% – 26,08% on the double dish system. The yeasts caused abnormal growth of the Colletotrichum sp. hyphae, such as swollen, curly, rolled, and lysis. A. pullulans Dmg 11 DEP caused the highest inhibition, with the category of strong antifungal activity on dual culture, and moderate on double dish system.  It was concluded that the three yeast isolates have the ability to inhibit the in vitro growth of Colletotrichum sp. the caused of  anthracnose on avocado fruit.