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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Journal of Marine and Aquatic Sciences Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Perikanan Universitas Gadjah Mada Jurnal Akuakultur Sungai dan Danau Jurnal Riset Akuakultur Jurnal Medik Veteriner Jurnal Penamas Adi Buana Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Journal of Marine and Coastal Science Jurnal Litbang Provinsi Jawa Tengah Berkala Penelitian Hayati Journal of Science and Science Education (JoSSEd) Sewagati Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Journal of Aquaculture Science
Akhmad Taufiq Mukti
Departemen Akuakultur, Fakultas Perikanan Dan Kelautan, Universitas Airlangga, Jl. Mulyorejo, Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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PEMBERDAYAAN SISWA-SISWI SEKOLAH MENENGAH KEJURUAN NEGERI PERIKANAN DALAM PEMBENIHAN IKAN AIR TAWAR Akhmad Taufiq Mukti; Muhamad Amin; Putri Desi Wulan Sari; Veryl Hasan; Gunanti Mahasri; Puguh Yugo Wijanarko; Lia Oktavia Ika Putri; Salman Aldo Alfaresi
Jurnal Penamas Adi Buana Vol 8 No 01 (2024): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol8.no01.a8740

Abstract

The limited knowledge and skills in the hatchery technology application of the freshwater fish to seed production business are obstacles faced by prospective young entrepreneurs in the aquaculture sector. The Community Partnership Program (PKM) was provided as a strategic step to overcome the obstacles faced by the target partner group in this PKM activity in line with the Public Vocational Secondary School's (SMKN) expectations to provide sufficient insight into science and technology for SMKN students as provision for independent entrepreneurship. The aim of this PKM was to transfer the application of freshwater fish (catfish) hatchery technology and the produce fish seeds independently. The activity method was conducted through: a) delivery of material and training related to broodstocks selection and gonad maturation, b) training on the application of freshwater fish hatchery technology (catfish), and c) regular monitoring and assistance to PKM target partner groups by involving related agencies (SMKN). Delivery of material was carried out regularly, both online and face to face (offline). Approximately 40 students of SMKN Ngadirojo, Pacitan Regency who took the flagship program of Aquaculture Agribusiness, attended this activity. This PKM activity was also attended by accompanying teachers. The practical results show an increase in the knowledge and skills of partners in catfish hatchery through the application of semi-artificial catfish broodstocks spawning technology.
Preliminary Study: Effect of Infrared Soft-Laser Irradiation on Gonadal Maturity Stage of Female Siamese Striped Catfish (Pangasianodon hypophthalmus) Puspitasari, Febriyanti; Mukti, Akhmad Taufiq; Mubarak, Ahmad Shofy
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.52410

Abstract

Gonadal maturity and spawning of Siamese striped catfish depend on the rainy season, while outside of the season, hormonal treatment is not effective. Alternative technologies should be explored to enhance the gonadal maturity of fish. This study aims to examine the effect of infrared soft-laser irradiation on the gonadal maturity stage (GMS) of female Siamese striped catfish (Pangasianodon hypophthalmus). This study was performed through experimental methods using a completely randomized design (CRD) consisting of six treatments with four replications. Treatments were used, namely negative control (K-, no treatment), positive control (K+, injection of human chorionic gonadotropin / hCG and ovaprimTM), soft-laser exposure dose of 0.2 J/cm2 (T1), 0.4 J/cm2 (T2), 0.6 J/cm2 (T3), and 0.8 J/cm2 (T4). The parameter of GMS was measured. Data was analyzed using ANOVA following Duncan’s multiple range test. The result showed that soft-laser irradiation showed significant differences (P < 0.05) in GMS of female Siamese striped catfish. The highest GMS was shown through soft-laser exposure dose of 0.4 J/cm2 (T2), with 98.75% of fish reaching GMS IV and 1.25% at GMS III. Infrared soft-laser irradiation dose of 0.4 J/cm2 was the optimal dose to accelerate the gonadal maturity of female Siamese striped catfish.
Application of Cellulase Enzyme in Feed on the Growth of Siamese Catfish (Pangasius hypophthalamus) Anjasmara, L. Dede Arta Satya; Amin, Muhamad; Mukti, Akhmad Taufiq; Mahasari, Gunanti; Agniny, Siswi Fililmi; Ali, Muhamad
Journal of Science and Science Education Vol. 6 No. 2 (2025): October
Publisher : Pascasarjana, Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jossed.v6i2.11894

Abstract

Siamese catfish (Pangasius hypophthalamus) is one of the most economically importantfreshwater commodities in Indonesian aquaculture. A major challenge during the growth-out phase, is the limited ability to digest plant-based diets due to high cellulose and crude fiber content, which reduce feed digestibility. Supplementation of cellulase in feed has been proposed as a strategy to improve nutrient utilization. Therefore the present study investigated the effect of dietary celulase supplementation on feed digestibility and growth performance in Seamese catfish. Juvenile catfish with an average initial length of 6cm were reared for 30 days in floating net in a cement pond. A complete reandomized design was employed with four treatments, two control, and four replciates. Treatments included cellulase derived from mivrobes sources T1 = Plant feed + WT, T2 = Plant feed + (WT + SKIK), T3 = Plant feed + Mutant, T4 = Plant feed + (mutant + SKIK). The control consisted of C1 = Commercial feed and C2 = Plant-based feed without enzyme supplementation. The results showed that C1 showed the best results for the growth of fish. However, T2, T3 and T4 showed the best results when compared to C2 and T1 which used 100% vegetable protein feed. Therefore, it can be concluded that the addition of cellulase enzymes to the feed has an effect on increasing the growth of Siamese catfish.
Pengaruh Penembakan Laser Inframerah pada Titik Reproduksi Terhadap Tingkat Kematangan Gonad, Fertilitas, Daya Tetas Telur dan Sitasan Larva Ikan Patin Siam Betina Puspitasari, Febriyanti; Mukti, Akhmad Taufiq; Mubarak, A Shofy
Grouper Vol. 14 No. 2 (2023): Grouper: Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v14i2.211

Abstract

Laserpunktur adalah salah satu terapi foto-biomodulasi yang memancarkan cahaya dengan radiasi elektromagnetik dan bersifat koheren yang dapat diterapkan pada ikan. Pemijahan buatan dengan menggunakan bantuan laserpunktur pada umumnya diberikan pada ikan yang mengalami kesulitan berkembang biak dengan sempurna di lingkungan buatan. Adapun tujuan lainnya, yaitu untuk memperoleh benih ikan di luar musim pemijahan, meningkatkan efisiensi produksi, meningkatkan kelangsungan hidup larva ikan. Penelitian ini bertujuan untuk mengetahui pengaruh laserpunktur terhadap tingkat kematangan gonad, fertilitas, daya tetas telur dan sitasan larva ikan patin siam betina. Penelitian ini menggunakan ikan patin siam betina dengan berat 0,6-0,8 kg/ekor sebanyak 24 ekor. Perlakuan yang digunakan dalam penelitian ini adalah kontrol negatif (tanpa penambahan apapun), kontrol positif (dengan penambahan ovaprim dan HCG), P1 (perlakuan dengan dosis iradiasi 0,2 Joule), P2 (perlakuan dengan dosis iradiasi 0,4 Joule), P3 (perlakuan dengan dosis iradiasi 0,6 Joule) dan perlakuan P4 dengan dosis iradiasi 0,8 Joule). Data dianalisis menggunakan Analysis of Variance (ANOVA) dan jika menunjukkan hasil yang signifikan maka perhitungan dilanjutkan dengan Duncan's Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa perlakuan terbaik yang mempengaruhi kematangan gonad, fertilitas, daya tetas telur dan sitasan larva adalah P2 (perlakuan dengan dosis penyinaran 0,4 Joule).
Potential of Antioxidant Activity Caulerpa racemosa Extract Using DES Solvent and Different Sonication Times as An Antibacterial Against Pathogenic Bacteria Satyantini, Woro Hastuti; Mukti, Akhmad Taufiq; Bakhri, Saiful
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.53303

Abstract

Caulerpa racemosa, a type of green algae, has been studied  for its potential health benefits, including antioxidant and an- tibacterial properties. The extraction of bioactive compounds  from marine algae using green solvents like Deep Eutectic Sol- vents (DES) has gained interest due to their efficiency and en- vironmental friendliness. This research aimed to determine  the potential antioxidant activity of C. racemosa extract using DES solvents and different sonication times as an antibacterial  against pathogenic bacteria. The research employed a Facto- rial Completely Randomized Design (CRD) with two variables:  sonication time (10, 20, and 30 minutes) and different DES solvents (ethanol and ethylene glycol (1:20) with 90% aquades, and methanol and glycerol (1:10) with 90% aquades. The highest antioxidant activity was found in DES  Methanol-Glycerol (36.39 mg TE/g dw), followed by DES Eth- anol-Ethylene glycol (28.30 mg TE/g dw), both significantly  different (p<0.05). The C. racemosa extract inhibited the growth of V. parahaemolyticus with an inhibition zone of 8.08 mm at 50 ppm and 13.13 mm at 1,000 ppm. For V. harveyi, inhibition started at 6.37 mm at 50 ppm, reaching 11.70 mm  at 1,000 ppm. DES Methanol-Glycerol with a 10-minute soni- cation provided the highest antioxidant activity and effectively  inhibited V. parahaemolyticus and V. harveyi. Extraction of C. racemosa with DES-MG solvent and varying sonication times influences its antioxidant activity and antibacterial properties.
PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF TENGKAWANG BUKIT (Shorea beccariana) STEM EXTRACT AGAINST Vibrio parahaemolyticus Anisa, Hosia; Mukti, Akhmad Taufiq; Mahasri, Gunanti; Amin, Muhamad; Aminah, Nanik Siti
Jurnal Riset Akuakultur Vol 20, No 3 (2025): September (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.3.2025.245-255

Abstract

Vibrio parahaemolyticus infection poses a significant challenge in intensive shrimp culture, leading to substantial economic losses. Its common treatments using synthetic antibiotics have been linked to increased risks of antibiotic resistance and residual effects. Therefore, finding environmentally safe and effective natural alternatives is deemed essential. Tengkawang Bukit (Shorea beccariana) stem extract contains antibacterial compounds, including asiatic acid, oleanolic acid, and lupanone, all classified as terpenoids. This study aims to evaluate the antibacterial potential of S. beccariana stem extract through phytochemical screening and a disc diffusion test against V. parahaemolyticus. The screening results confirmed the presence of bioactive compounds, including alkaloids, flavonoids, saponins, tannins, and steroids. The disc diffusion test showed an increase in the inhibition zone with increasing extract concentration, with inhibition diameters ranging from 9.04 ± 0.48 mm to 10.75 ± 0.26 mm. The 17% extract yield indicates a high availability of active compounds. These findings suggest that tengkawang bukit stem extract has potential as a natural antibacterial alternative for controlling V. parahaemolyticus in shrimp culture. This approach could help reduce reliance on synthetic antibiotics and promote sustainable fisheries. Infeksi Vibrio parahaemolyticus merupakan salah satu kendala utama dalam budidaya udang yang menyebabkan kerugian ekonomi signifikan. Penggunaan antibiotik sintetis sebagai pengobatan telah menimbulkan kekhawatiran terkait resistensi dan residu, sehingga diperlukan alternatif alami yang aman dan efektif. Batang tengkawang bukit (Shorea beccariana) diketahui mengandung senyawa antibakteri seperti asam asiatik, asam oleanolik, dan lupanon yang tergolong ke dalam senyawa terpenoid. Studi ini bertujuan untuk mengevaluasi potensi antibakteri ekstrak batang S. beccariana melalui skrining fitokimia dan uji difusi cakram terhadap V. parahaemolyticus. Hasil skrining menunjukkan adanya kandungan senyawa bioaktif berupa alkaloid, flavonoid, saponin, tanin, dan steroid. Uji difusi cakram memperlihatkan zona hambat yang meningkat seiring dengan peningkatan konsentrasi ekstrak, dengan diameter hambatan berkisar 9,04 ± 0,48 mm hingga 10,75 ± 0,26 mm. Rendemen ekstrak sebesar 17% menunjukkan ketersediaan senyawa aktif yang melimpah. Hasil riset ini mengindikasikan bahwa ekstrak batang tengkawang bukit berpotensi sebagai alternatif antibakteri alami dalam pengendalian V. parahaemolyticus pada budidaya udang, sehingga dapat mengurangi ketergantungan terhadap antibiotik sintetis dan mendukung keberlanjutan perikanan.
PENINGKATAN AKTIVITAS DAN EKSPRESI PROTEIN TOTAL ENZIM SELULASE REKOMBINAN SERTA POTENSI APLIKASINYA PADA PAKAN IKAN Agniny, Siswi Fililmi; Mahasri, Gunanti; Mukti, Akhmad Taufiq; Amin, Muhamad
Jurnal Riset Akuakultur Vol 20, No 3 (2025): September (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.3.2025.257-267

Abstract

Meskipun enzim selulase rekombinan banyak dikembangkan untuk meningkatkan efisiensi pemecahan serat, informasi mengenai bagaimana modifikasi seperti mutasi dan penambahan tag SKIK (Ser-Lys-Ile-Lys) dapat memengaruhi aktivitas enzim dan produksi protein total masih terbatas. Oleh karena itu, penelitian ini bertujuan mengevaluasi pengaruh mutasi dan penambahan tag SKIK terhadap aktivitas dan protein total enzim selulase rekombinan yang diproduksi oleh Escherichia coli BL21 (DE3). Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan, yaitu P1= wild type (WT), P2 = WT + SKIK, P3 = mutan, dan P4 = mutan + SKIK, masing-masing dengan empat ulangan. Produksi enzim dilakukan melalui kultur E. coli rekombinan yang membawa plasmid pET-22b-CellE dengan induksi laktosa monohidrat, kemudian dianalisis menggunakan uji aktivitas selulase metode 3,5-dinitrosalisilat (DNS) dan pengukuran protein total dengan metode Bradford. Data dianalisis menggunakan ANOVA satu arah dan uji lanjut Duncan pada tingkat signifikansi 5% (p < 0,05). Hasil penelitian menunjukkan bahwa perlakuan mutan + SKIK menghasilkan nilai tertinggi baik pada aktivitas enzim (10,66 U mL-1) maupun protein total (0,85 mg mL-1). Penambahan SKIK meningkatkan ekspresi dan kelarutan protein, sedangkan mutasi memperbaiki efisiensi katalitik enzim. Kombinasi keduanya memberikan efek sinergis dalam meningkatkan perfoma enzim selulase rekombinan. Penelitian ini merupakan yang pertama mengombinasikan penambahan tag SKIK dengan mutagenesis untuk meningkatkan ekspresi dan aktivitas katalitik enzim selulase pada E. coli. Temuan ini tidak hanya relevan untuk peningkatan produksi enzim pada industri bioteknologi, tetapi juga berpotensi diaplikasikan dalam formulasi pakan ikan. Although recombinant cellulase enzymes have been widely developed to enhance fiber-degradation efficiency, information regarding how modifications such as mutagenesis and the addition of the SKIK (Ser-Lys-Ile-Lys) tag influence enzymatic activity and total protein production remains limited. Therefore, this study aimed to evaluate the effects of mutagenesis and SKIK tagging on the activity and total protein yield of recombinant cellulase expressed in Escherichia coli BL21 (DE3). The research was conducted experimentally using a completely randomized design (CRD) with four treatments: P1 = wild type (WT), P2 = WT + SKIK, P3 = mutant, and P4 = mutant + SKIK, each consisting of four replications. Enzyme production was carried out using recombinant E. coli harboring the pET-22b-CellE plasmid induced with lactose monohydrate, and the resulting enzyme was analyzed for cellulase activity using the 3,5-dinitrosalicylic acid (DNS) method and total protein concentration using the Bradford assay. Data were analyzed using one-way ANOVA followed by Duncan’s post-hoc test at a 5% significance level (p < 0.05). The results showed that the mutant + SKIK treatment produced the highest values for both enzymatic activity (10.66 U mL-1) and total protein (0.85 mg mL-1). The addition of the SKIK tag enhanced protein expression and solubility, whereas mutagenesis improved catalytic efficiency. The combination of both modifications produced a synergistic effect, resulting in superior performance of the recombinant cellulase enzyme. This study is the first to combine SKIK tagging with mutagenesis to enhance the expression and catalytic activity of cellulase in E. coli. These findings are not only relevant for improving enzyme production in biotechnology industries but also hold potential applications in fish feed formulation.
Fekunditas, Diameter Telur, Persentase Fertilitas, Dan Daya Tetas Pada Strain Ikan Cupang (Betta Splendens) Yang Berbeda Darmawan Setia Budi, S.Pi., M.Si; Dian Pebianti; Akhmad Taufiq Mukti; Laksmi Sulmartiwi; Lailatul Lutfiyah; Mohammad Faizal Ulkhaq
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.273

Abstract

Betta fish (Betta splendens) has export and import potential. Several popular betta fish strains include halfmoon, crown tail, double tail, and placard. We used the observation method to observe four betta fish strains with five replications. The four fish strains were spawned and will be dissected to obtain the female betta fish's gonads. The parameters observed in this study were fecundity, egg diameter, fertility, and hatchability. This study's data analysis method was analysis of variance (ANOVA) with Duncan's multiple range test. Based on the findings of the observations, it is known that the results of betta fish spawning and gonadal dissection on these various betta fish strains show a significant difference in fish fecundity (P 0.05). However, no significant difference in egg diameter, fertility, or hatchability was observed (P > 0.05). Betta splendes strains differ in terms of fecundity, fertility, hatching rate, and egg size.
Individual Growth And Gonad Development Of Female Tilapia (Oreochromis Sp.) With Addition Of Velvet Bean (Mucuna Pruriens) Seed Extract Ahmad Shofy Mubarak; Kamiliya Zahrah Taher; Putri Eriza Riyanto; Akhmad Taufiq Mukti; Muhamad Amin; Syifania Hanifah Samara
Journal of Aquaculture Science Vol 8 No 2 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v8i2.48698

Abstract

Tilapia is one of the fish that is widely cultivated in Indonesia. The obstacle to tilapia cultivation is high reproduction, which inhibits growth. An alternative to increase growth is by inhibiting gonad growth, so that feed energy is focused on somatic development. The aim of this research was to analyse the effect of velvet bean extract concentration on individual growth and gonad development of female tilapia fish and to determine the optimum concentration of velvet bean extract to increase growth and reduce reproductive activity in female tilapia fish. This study used a Completely Randomized Design (CRD) with the addition of various velvet bean extracts and compared with the control (without velvet bean extract). The use of velvet bean extract had a significant effect (p<0.05) on growth parameters such as length, weight, fecundity and egg diameter. The best treatment was found in treatment 3 (velvet bean extract 5 ml.kg-1 ) which resulted in growth in individual length of 16.1 cm, individual weight of 89.43 g, fecundity of 2206 and egg diameter of 1.11 mm. In addition, the dose in this treatment may reduce the reproductive activity of tilapia.
Conservation Challenges for Macrognathus acuelatus: A Case Study of Threatened Fish in Bengawan Solo and Brantas Rivers, East Java Nida'an Khofiyyah; Akhmad Taufiq Mukti; Laksmi Sulmartiwi
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.51654

Abstract

Macrognathus aculeatus is a local fish from the Bengawan Solo and Brantas Rivers threatened with extinction. Due to the lack of literacy on the biology of this fish. Biological parameters can be used to determine the condition of this fish in nature. Field observations at five stations from the two rivers were conducted to obtain samples. Biological and reproductive parameters such as weight and length, gonad maturity level (GML), Gonadosomatic Index (GSI), Hepatosomatic Index (HSI), and Condition Factor (CF) were the parameters observed in this study. All data obtained were presented as mean and standard deviation. Furthermore, analysis of variance was conducted using ANOVA (p < 0.05) to determine differences in samples from each observation station. The results showed that some biological factors had significant results (p < 0.05) on Gonadosomatic Index and Hepatosomatic Index). However, there was no significant difference (p > 0.05) in the condition factor. On the other hand, there were significant differences (p < 0.05) in body weight, total length, ovary weight, and fecundity parameters across all sampling sites.
Co-Authors A. Shofy Mubarak Adriansyah, Ivan Agniny, Siswi Fililmi Agung Pamuji Rahayu Alim Isnansetyo Alvira Febrianti Pratiwi Anisa, Hosia Anjasmara, L. Dede Arta Satya Arafik Lamadi Ardiyan, Buyung Raga Aulia Ikhsan Syamsuri Aziz, Ahmad Saiful Boedi Setya Rahardja Browijoyo Santanamurti Catur Pujiono Danita Garneda Rezi DARMAWAN SETIA BUDI Denta Batara Kusuma Hendra Destya Twinandia Diah Ayu Safitri Dian Pebianti Diki Firdiansyah Dyah Hariani Dyah Hariani Edi Rukyanto Endah Sih Prihatini Endang Tri Wahyurini Fachriza Tri Aprilia Falatehan, Naufal Fasya, Arif Habib Firdaus, Fitrul Azizi Fuquh Rahmat Shaleh Gunanti Mahasri gustini, Maria A Herliana Arsianingtyas Imlani, Ainulyakin Isroni, Wahyu Jati, Ilham Shofananda Pramono Kamiliya Zahrah Taher Kenconojati, Hapsari Kismiyati , Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Lana Nafisyah, Ayu Lia Oktavia Ika Putri Lutfianasari, Lutfianasari Luthfiana Aprilianita Sari M Wahyu Alfian Mahasari, Gunanti Maria A gustini Mariah, Sharven Rao Mike van Keulen Mohammad Faizal Ulkhaq Muhamad Ali Muhamad Amin Muhamad Amin Muhamad Amin Muhammad Arief Muhammad Arief Mutiara Rahmawati Nanik Siti Aminah Naufal Abiyu Pratama Nida'an Khofiyyah Nina Nurmalia Dewi Nizar Afiansyah Loekman Panhar, Fio Surya Pratama Semendo Puguh Yugo Wijanarko Pungky Slamet Wisnu Kusuma Puspitasari, Febriyanti Putri Eriza Riyanto Rahmadhani, Desi Rahmahani, Jola Rahmawan, Dani Bagus Rozi Rozi Rr. Juni Triastuti Sabrina, Annisa Nur Saiful Bakhri Salman Aldo Alfaresi Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Sapto Andriyono Sari, Luthfiana Aprilanita Sari, Putri Desi Wulan Septuresty Hartri Eka Setyantini, Woro Hastuti South, Josie Sri Harweni Sri Subekti Suciyono, Suciyono Sudarno, Sudarno Veryl Hasan Vivaldy Phaza Muharta Yudi Cahyoko