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Biodegradability of Proteolytic Bacteria in Mangrove Ecosystems Claudia, Kristiwany Mayneke; Nursyirwani, Nursyirwani; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 2 No. 2 (2021): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

The purpose of this study was to awareness the optimal time for the growth of proteolytic bacteria in producing the protease enzyme and determine the effectiveness of the protease enzyme from proteolytic bacteria in degrading mangrove litter. The method used was the experimental method by using two proteolytic bacteria isolates, namely Bacillus manliponensis (K6) and Bacillus toyonensis (K20). This method used one treatment for each proteolytic bacterial isolate, namely the addition of different extract containing protease enzyme (0%, 25%, and 50%) with three replications. Measurement of bacterial growth was carried out every 6 hours for 24 hours using Total Plate Count (TPC) and spectrophotometric method. B. manliponensis and B. toyonensis have enzyme activity as indicate by the presence of a clear zone on Zobell Marine Agar 2216 and skim milk 1%. The growth bacteria based on the TPC measurement was directly proportional to the spectrophotometric measurement results. The highest bacterial count was (1.39 x 108 CFU's/ml) K6 bacteria and (1.52 x 108 CFU's/ml) K20 bacteria. The results of the measurement of cell growth in the spectrophotometric method occurred at the 6th hour, namely 10.36 x 108 CFU's/ml (K6 bacteria) and 10.97 x 108 CFU's/ml (K20 bacteria). The optimum time of the protease enzyme occurred at 6 hours, which was 0.0258 mg/ml (K6 bacteria) and 0.0262 mg/ml (K20 bacteria). The highest dissolved protein content was obtained at the 50% dose of 0.054 mg/ml (K6 bacteria) and 0.055 mg/ml (K20 bacteria), while the lowest was at 0%, namely 0.050 mg/ml (K6 bacteria) and 0.051 mg/ml (K20 bacteria).

Analysis of Indigenous Bacteria as Microplastic Degradation of Sediment in the Sea Waters of Dumai, Riau Province Pakpahan, Deni; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 2 No. 3 (2021): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Nowdays Marine pollution resulted by microplastics is a global concern. Microplastic particles have the potential to cause damage to biota. One of solution to overcome the problem of marine pollution by microplastics by applying indigenous bacteria as microplastic degradation. This study was conducted to determine the type and ability of indigenous bacteria in sediments that are able to degrade microplastics. The research was conducted from October to December 2020. Sediment sampling was conducted in Dumai Sea Waters and sample analysis was conducted at the Marine Microbiology Laboratory, Faculty of Fisheries and Marine, Riau University. Indigenous bacteria from sediments were isolated in bacterial breeding media and then tested degradation capabilities on PET plastic samples. The result of the degradation test obtained ISL8 was able to degrade the mycoplastic with a percent degradation about 7%. Molecular test of ISL8 using PCR (Polymer chain reaction) 16S rRNA method and GenBank analysis is known that ISL8 is Bacillus sp.

Isolation and Identification of Bacteria from Dumai Marine Waters that Have Potencial as Lead Bioremediation Agents Yanti, Della Hijri; Nursyirwani, Nursyirwani; Yoswaty, Dessy
Journal of Coastal and Ocean Sciences Vol. 2 No. 3 (2021): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

One of the harmful pollutants to human health is the heavy metal Lead (Pb). A high concentration of lead that has can accumulate in the body of living things if in a long period of time. Lead concentrations that exceed the threshold in an environment can cause damage that affects aquatic biota and other living things. This research was carried out in February-April 2021. The purpose of this study was to isolate and identify bacteria that have the potential as lead bioremediation agents from Dumai marine waters. Isolation and identification of bacteria were carried out at the Laboratory of Marine Microbiology, Department of Marine Science, Faculty of Fisheries and Marine Sciences, and the lead concentration test at the Laboratory of Chemical Testing and Analysis, Department of Chemical Engineering, Faculty of Engineering. The results of the isolation on Marine Agar media which was added with Pb acetate (Pb(CH3COO2)) obtained three bacterial isolates from Dumai sea waters, namely D1, D2, and D3. The isolate that had the best growth was isolated D1. The isolate D1 was able to reduce the highest Pb concentration from 10 ppm by 80.53%, at a concentration of 20 ppm Pb, the isolate D1 was able to reduce Pb concentration by 78.8% and at a concentration of Pb 30 ppm, isolate D1 was able to reduce Pb concentration by 77.21%.

Analysis of Lead and Copper Concentrations in Mangrove Clams (Geloina erosa) and Sediments in The North Coast of Bengkalis Island Gunova, M Taufik; Amin, Bintal; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 3 No. 2 (2022): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

This research was conducted in February-April 2021 by taking samples of mangrove clams (Geloina erosa) and sediment from Selat Baru and Teluk Pambang coastal area in Bantan District, Bengkalis Regency, Riau Province. The purpose of this study was to determine the concentration of heavy metals, namely Pb and Cu, in sediment and mangrove clams that are abundant in the studied area. The research method used is the survey method and samples were taken by means of the purposive sampling technique. Heavy metal concentrations were analyzed by using Atomic Absorption Spectrophotometer (AAS). The results showed that the concentrations of Pb and Cu in the mangrove clams and sediments in the studied area had not yet passed the threshold level. Pb and Cu concentrations found on clams were 0.0468 μg/g and 0.1043 μg/g respectively. Meanwhile, the concentration of Pb in the sediment was 0.10185 μg/g and Cu 0.3313 μg/g. Mangrove clams in the north coast of Bengkalis Island have the ability to accumulate Pb and Cu with a low ability (BCF<1). The maximum Tolerable Intake of the clams for humans with a bodyweight of 70 kg is 0.0983 kg/week (Lead) and 7.3010 kg/week for Copper

Antibacterial Activity of Avicennia alba Leaves and Stem Extracts Against Pathogenic Bacteria (Pseudomonas aeruginosa, Aeromonas salmonicida, Staphylococcus aureus) Purba, Paedo Yayaki; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 3 No. 2 (2022): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

This research was conducted in November 2020-February 2021 at the Laboratory of Fish Quarantine and Quality Control (BKIPM) Pekanbaru, Riau. The aim of this research was to test the activity of A. alba mangrove leaf extracts and stems in inhibiting the growth of pathogenic bacteria P.aeruginosa, A.salmonicida, and S.aureus. The research method used in this study is the experimental method. The sampling was conducted at the location of Marine Station UNRI Purnama Dumai. The results showed that A. alba leaf extract has the highest inhibitory power at a concentration of 100% with an average of 4.37–5.13 mm. The highest inhibitory power was against S.aureus (5.13 mm) and the lowest inhibition power was found in A. salmonicida (4.37 mm). The lowest inhibition was found at a concentration of 12.5% with an average of 0.45–0.67 mm. The stem extract of A. alba had the highest inhibitory power at a concentration of 100% with an average of 6.17–7.47 mm. The highest inhibition power was against S. aureus 7.47 mm and the lowest inhibition power was found in A.salmonicida (6.17 mm). The lowest inhibitory was at a concentration of 12.5% with an average of 1.52–2.29 mm. Based on the criteria for the strength of antibacterial activity of stem extract A. alba was in the medium category and the leaf extract was in the weak category

Nematocytes in the Coral Species Acropora formosa on Kasiak Island, Pariaman City, West Sumatera Pratama, Bima; Thamrin, Thamrin; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 6 No. 1 (2025): January
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jocos.6.1.39-43

This research was conducted in January 2024 on Kasiak Island, Pariaman City, West Sumatra Province. This research aims to identify nematocytes found in the body tissue of the Acropora formosa coral. The method used in this research is a survey method with direct observation and sampling in the field. Determination of the type of nematocytes from A. formosa coral was carried out using an Olympus microscope with an objective lens magnification of 10X40 (400) at the Marine Biology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. Of the 25 main types of nematocytes known in the phylum Cnidaria, one type was found in this species. The results of the research show that there is a type of Holotrichous isohiza in the A. formosa coral. This type of nematocyte is thought to be used to attack and kill prey

Nematocytes in the Coral Species Acropora formosa on Kasiak Island, Pariaman City, West Sumatera Pratama, Bima; Thamrin, Thamrin; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 6 No. 1 (2025): January 2025
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jocos.6.01.39-43

This research was conducted in January 2024 on Kasiak Island, Pariaman City, West Sumatra Province. This research aims to identify nematocytes found in the body tissue of the Acropora formosa coral. The method used in this research is a survey method with direct observation and sampling in the field. Determination of the type of nematocytes from A. formosa coral was carried out using an Olympus microscope with an objective lens magnification of 10X40 (400) at the Marine Biology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. Of the 25 main types of nematocytes known in the phylum Cnidaria, one type was found in this species. The results of the research show that there is a type of Holotrichous isohiza in the A. formosa coral. This type of nematocyte is thought to be used to attack and kill prey

Sensitivity Test of Pedada (Sonneratia alba) Leaf Extract Against the Growth of Staphylococcus aureus, Escherichia coli, and Aeromonas hydrophila Bacteria Wiya, Siti Ada; Wahyuni, Sri; Mursawal, Asri; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 6 No. 2 (2025): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jocos.6.2.114-118

Sonneratia alba (Pedada) is one of the potential mangroves as a natural antibacterial because it contains secondary metabolites, such as flavonoids, saponins, steroids, tannins, and terpenoids. This study aimed to determine the inhibitory power of Pedada leaf extract in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Aeromonas hydrophila. The research method used is experimental, including sample preparation, extraction, and sensitivity tests using the Kirby-Bauer disc method. This study used 100%, 50%, 25%, and 12.5% extract doses, positive control (Oxytetracycline), and negative control (a quade). The results showed that Pedada leaf extract could inhibit the growth of pathogenic bacteria; 100% concentration was the concentration with the widest inhibition zone diameter of S. aureus 15.48 mm, E. coli 13.37 mm, and A. hydrophila 13.88 mm. Based on this study's results, the best extract concentration for inhibiting bacterial growth is 100% concentration

Analysis of Lead and Copper Concentrations in Mangrove Clams (Geloina erosa) and Sediments in The North Coast of Bengkalis Island Gunova, M. Taufik; Amin, Bintal; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 3 No. 2 (2022): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

This research was conducted in February-April 2021 by taking samples of mangrove clams (Geloina erosa) and sediment from Selat Baru and Teluk Pambang coastal area in Bantan District, Bengkalis Regency, Riau Province. The purpose of this study was to determine the concentration of heavy metals, namely Pb and Cu, in sediment and mangrove clams that are abundant in the studied area. The research method used is the survey method and samples were taken by means of the purposive sampling technique. Heavy metal concentrations were analyzed by using Atomic Absorption Spectrophotometer (AAS). The results showed that the concentrations of Pb and Cu in the mangrove clams and sediments in the studied area had not yet passed the threshold level. Pb and Cu concentrations found on clams were 0.0468 μg/g and 0.1043 μg/g respectively. Meanwhile, the concentration of Pb in the sediment was 0.10185 μg/g and Cu 0.3313 μg/g. Mangrove clams in the north coast of Bengkalis Island have the ability to accumulate Pb and Cu with a low ability (BCF<1). The maximum Tolerable Intake of the clams for humans with a bodyweight of 70 kg is 0.0983 kg/week (Lead) and 7.3010 kg/week for Copper.

Antibacterial Activity of Avicennia alba Leaves and Stem Extracts Against Pathogenic Bacteria (Pseudomonas aeruginosa, Aeromonas salmonicida, Staphylococcus aureus) Purba, Paedo Yayaki; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Journal of Coastal and Ocean Sciences Vol. 3 No. 2 (2022): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

This research was conducted in November 2020-February 2021 at the Laboratory of Fish Quarantine and Quality Control (BKIPM) Pekanbaru, Riau. The aim of this research was to test the activity of A. alba mangrove leaf extracts and stems in inhibiting the growth of pathogenic bacteria P.aeruginosa, A.salmonicida, and S.aureus. The research method used in this study is the experimental method. The sampling was conducted at the location of Marine Station UNRI Purnama Dumai. The results showed that A. alba leaf extract has the highest inhibitory power at a concentration of 100% with an average of 4.37–5.13 mm. The highest inhibitory power was against S.aureus (5.13 mm) and the lowest inhibition power was found in A. salmonicida (4.37 mm). The lowest inhibition was found at a concentration of 12.5% with an average of 0.45–0.67 mm. The stem extract of A. alba had the highest inhibitory power at a concentration of 100% with an average of 6.17–7.47 mm. The highest inhibition power was against S. aureus 7.47 mm and the lowest inhibition power was found in A.salmonicida (6.17 mm). The lowest inhibitory was at a concentration of 12.5% with an average of 1.52–2.29 mm. Based on the criteria for the strength of antibacterial activity of stem extract A. alba was in the medium category and the leaf extract was in the weak category.

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