Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : hayati journal of biosciences

Development of Few Significant SNP Markers from Transcriptomic Data for Selection of Sengon (Falcataria falcata (L.) Greuter and R. Rankin) Resistant to Boktor Stem Borer and Gall Rust Disease Nugroho, Aditya; Anita, Vilda Puji Dini; Matra, Deden Derajat; Siregar, Iskandar Zulkarnaen; Siregar, Ulfah Juniarti
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.110-125

Abstract

Sengon (Falcataria falcata (L.) Greuter and R. Rankin) plantations in Indonesia are threatened by attacks from Boktor stem borers and gall rust disease. Controlling pests and diseases is difficult therefore, planting resistant trees obtained from tree selection programs is necessary. Currently, genomic breeding often incorporates GWAS, which uses thousands of SNP markers to identify markers with significant associations with the traits studied. This study aimed to bypass such expensive studies by identifying and developing SNP markers from sequences of putative resistance genes to Boktor stem borer and gall rust disease, identified from sengon transcriptomic data analysis. A total of 496,194 putative SNP sites were identified from transcriptomic sequences using the SAMtools and BFCtools programs, of which 119 SNP sites were associated with resistance genes. Of the 101 non-synonymous SNPs selected, only 12 were located in the conserved domain of each gene and were used for primer design. Of the 13 primers designed, only 10 were successfully amplified. Validation of 10 developed SNP markers on 100 sengon accessions using the HRM method confirmed a significant association between SNP markers and resistance traits, with a -log 10 (P-value) between 10.49 and 16.63. A few SNPs markers developed from putative resistance gene sequences are associated with resistance traits in sengon. Therefore, the SNP markers could be applied in selection programs for sengon trees resistant to Boktor stem borers and gall rust disease.
Co-Authors - Surachman, - Abdul Wahab Ainur Rofiq AlMarwan, Usmar Almizani, Avisena Ahsan Andri Sutrisno Anita, Vilda Puji Dini apri dwi anggo Basalamah, Zahira Aura Rahma Bawono, Yoseph Hari Bayu Winata, Bayu Dede J Sudrajat Dede J. Sudrajat Dede Jajat Sudrajat, Dede Jajat Endah Suhaendah Evayusvita Rustam, Evayusvita Fadhil, Dimas Nu'man Fadhil, Dimas Nu’man Farosandi, Naufal Hilmi Fitri Indriani Fronthea Swastawati Gugat, Rudy Max Damara Hamdi, Fuadi Hardiawan, Ridwan Henti Rosdayanti, Henti Hesti Ratnasari, Hesti Himawan, Erik Imam Wahyudi Indrawan, Imam Wahyudi Irenita, Novembriani Irviana, Risna ISKANDAR ZULKARNAEN SIREGAR Jaka S, Muhammad Surya Juanda Astarani Khaeran, Rafi Rizaldi Kholis, Abid Khafida Kinandy, Wafiq J. Lingga Wijaya, Harma Oktafia Luluk Setyaningsih Maharani, Dewi Matra, Deden Derajat Muhammad Efendi, Muhammad Mulyana, Ade Nendi Nonik Kusuma Ningrum Noor Farikhah Haneda Nur Lailatul Rahmah Nurianti, Esti Nurul Myristica Indraswari Parhusip, Veronica Prahma, Kelvin Dianda Bella Pramisari, Yunita Pratama, Risqi Fitriyadi Rizal Pratiwi, Sekar Widyastuti Prihadiati , Rr Lyia Aina Raga, Paulus Rahayu Puji Suci Rosi di Rudi Kurniawan Salim, Mohammad Agus Septianti, Riana Siahaan, Juaksa Siregar, Ulfah J Sri Wilarso Budi Sudinta, Heru Suharto Abdul Majid Surachman Surachman Suryobuwono, Abdullah Ade Talib, Nur Avifah Zahra Tampubolon, Irvan Adam Udin, Rizaldy Ulfah Juniarti Siregar Utama, Rony Edward Utami, Fitriana Putri Veithzal Rivai Zainal Wahyu Andhyka Kusuma Wawangningrum, Hary Wijana, . Susinggih Wulandari, Septiani Zulkifli Zulkifli