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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Disease Prevention and Public Health Journal Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Agroekoteknologi dan Agribisnis Berita Kedokteran Masyarakat Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Widagdo Sri Nugroho
Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Protektivitas Sapi di Kabupaten Kupang Terhadap Penyakit Ngorok (Septicaemia Epizootica) (PROTECTIVITY AGAINST SEPTICAEMIA EPIZOOTICA OF COWS IN KUPANG DISTRICT) Hilda Susiyanti Debora Berek; Widagdo Sri Nugroho; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Controlling SepticemiaEpizooticae (SE) through vaccination program has been undertaken in KupangDistrict. However, numbers of fatal cases is still being reported. A cross sectional study have been undertakenin order to determine the prevalence and factors affecting herd immunity at farm level in Kupang District.A total of 434 cow’s sera from 87 farmers were collected and further analyzed using Enzyme-linkedImmunosorbent Assay (ELISA). An antibody titer of e”200 ELISA Unit was used as the indicator ofprotective immunity. The prevalence of herd immunity was 73.7% and vaccination coverage was 90.8%.Based on unweighted logistic regression analysis it was found that factors affecting the animals protectiveimmunity were: animal >2 years of age (r = +1.45601; OR = 4.3); housing system ( r = +1.03958; OR = 2.8);reservoir animals (r = +0.090147; OR = 2.5), the sex of the animal (female) (r = + 0.080138; OR =2.2); oncefrequency of vaccination (r = +0.61015; OR = 1.8); and 6-12 months post vaccination period (r = + 0.58968;OR = 1.8). Based on linier regression analysis, the prevalence of herd immunity was increased during rainyseason, whereas it was decreased when cows werebought from animal market or from other district.
Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Widodo Suwito; Widagdo Sri Nugroho; Bambang Sumiarto; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).
Deteksi Mycobacterium Avium Subspesies Paratuberculosis pada Susu Pasturisasi yang Dijual di Bogor Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Ewald Usleber
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mycobacterium avium subspesies paratuberculosis (MAP) is a thermal tolerant bacteria. The presenceof these bacteria in pasteurized dairy milk is associated with infectious bowel disease in human known asCrohn’s disease. The aim of this study was to detect MAP in pasteurized dairy milk sold in Bogor. Fourtytwo samples of plain flavoured milk (180–250 ml) from 7 producers were bought from supermarkets inBogor. The presence of MAP was detected by isolation and conventional polymerase chain reaction (PCR)using IS 900 and F57. Bacterial isolation were done by Herrold’s egg yolk medium with mycobactine J(HEYMj) and without mycobactin J (HEYM) and incubated at 37°C for 20 weeks. The DNA extraction ofall pasteurized dairy milk samples were conducted by DNeasy® Tissue Kit. Amplification conditionsfor PCR were: 1 cycle at 94°C for 10 minutes, 40 cycles at 94°C for 1 minute, 58°C for 1 minute, and72°C for 3 minutes, and 1 cycle at 72°C for 7 minutes. After 20 weeks of incubation, there were no sign ofMAP which grew in all isolation mediums. The PCR IS 900 and F57 did not detect the DNA band of thetarget. In the conclusion, there was no MAP detected in pasteurized dairy milk sold in Bogor.
Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Mazdani Ulfah Daulay; Mirnawati Sudarwanto; Widagdo Sri Nugroho; Etih Sudarnika
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.
Co-Authors Abdulwahed Ahmed Hassan AETH Wahyuni Wahyuni, AETH Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Aris Haryanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih D.W Lukman Defryana Rakebsa Denny Widaya Lukman Dian Bekti Hadi Masithoh Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih E. Setiyaningsih E. Usleber Erwan Agus Purwanto Etih Sudarnika Ewald Usleber F. Lintang R. Daru Maya Fitriana, Ida Gallantiswara, Putu Cri Devischa Hari Kusnanto Heris Kustiningsih Heru Susetya Hilda Susiyanti Debora Berek Ismadi, Joko Kajang, Elphan Augusta Khrisdiana Putri Khrisdiana Putri, Khrisdiana Kusnanto, Hari M. Haryadi Wibowo M. Sudarwanto Maftuhah Nurbeti, Maftuhah Marla Anggita Mazdani Ulfah Daulay Minarwati Sudarwanto Mirnawati Sudarwanto Nurbeti, Maftuhah Prisha Lini Sivalingham Puspa Wikan Sari Raden Gagak Donny Satria Rahmat Setya Adji Rahmat Setya Adji Rahmi, Siwi Meylina Rochman Naim Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Salim, Devi Andreani Setiawan, Dwi Cahyo Budi Setyawan Budiharta Silalahi, Gustaf Eifel Surachmi Setyaningsih Teguh Budipitojo Toha, Larry Richard Wellem Widodo Suwito Widodo Suwito Widodo Suwito Widya Asmara Yatri Drastini Yuda Heru Fibrianto Yudhanto, Setyo