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Training on Processing Natural Resources into Processed Food and Health Products for Mothers in Tondongggeu Village, Nambo District, Kendari City Ambardini, Sri; Ahmad, Sitti Wirdhana; Muhsin, Muhsin; Karya, Adi; Syah, Muhamad Azwar; Adi Parman Rudia, La Ode; Yanti, Nur Arfa
Majalah Pengabdian Indonesia Vol. 1 No. 3 (2024): Desember 2024
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v1i3.32

Abstract

This community service activity is a training activity on the manufacture of health and food products that have the opportunity to improve the community's economy as well as providing education on the use of moringa leaves as face masks and papaya fruit as an analog tomato sauce to mothers in Tondonggeu Village, Nambo District, Kendari City. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Papaya as a substitute for tomatoes in making analog tomato sauce can be done because papaya has characteristics like tomatoes, namely color, organic acid content, and viscosity, even papaya has a higher vitamin C level than tomatoes.  Moringa leaves contain many vitamins, including vitamin A, vitamin C, amino acids and antioxidants such as flavonoids, quercetin and zeatin which function to maintain dry skin hydration and prevent the appearance of wrinkles, because it helps fight free radicals, so it is very good to be used as a cosmetic ingredient in the form of a face mask. Participants in this training involved residents of Tondonggeu village, Thematic KKN students and a team of accompanying lecturers from the Biotechnology and Biology study program of FMIPA Halu Oleo University. The training methods used are lectures, demonstrations and discussions. The lecture method was carried out with the aim of providing information and knowledge about moringa masks and analog ketchup. The demonstration aims to provide skills to mothers through the practice of directly processing moringa leaves into masks and papaya fruits into analog tomato sauce while the discussion is intended to show the activeness and understanding of the community during the training process. The results of the training on making masks and analog tomato sauce carried out by mothers are in the good category with an average success of 85%, their response to the implementation of the training is very good as seen from the enthusiasm of the residents to participate in the activity from the beginning to the end. This training activity can increase knowledge and improve mothers' skills in producing moringa masks and papaya analogue tomato sauce.
Skrining Bakteri Termohalofilik Penghasil L-asparaginase dari Sumber Air Panas Wawolesea Sulawesi Tenggara dan Uji Aktivitas Enzimnya Muzuni Muzuni; Jamaluddin Jamaluddin; Suriana Suriana; Ardiansyah Ardiansyah; Nur Arfa Yanti
ALCHEMY Jurnal Penelitian Kimia Vol 20, No 1 (2024): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.20.1.73523.12-21

Abstract

L-asparaginase merupakan enzim yang mengubah L-asparagin menjadi L-aspartat. L-asparagin dapat dimanfaatkan oleh sel kanker leukemia sebagai salah satu sumber nutrisinya. Penambahan L-asparaginase dapat menghambat pertumbuhan sel kanker. Penggunaan L-asparaginase dalam skala industri lebih mengutamakan L-asparaginase yang memiliki aktivitas dan stabilitas optimum di suhu tinggi, karena kecepatan reaksi dalam menghidrolisis L-asparagin tinggi, stabil dari denaturan misalnya detergen dan senyawa organik, dan stabil pada kondisi asam maupun basa. L-asparaginase yang memiliki aktivitas dan stabilitas di suhu tinggi dapat dieksplorasi dari mikroorganisme yang hidup di lingkungan yang bersuhu dan bersalinitas tinggi. Tujuan penelitian ini adalah untuk memperoleh isolat bakteri termohalofilik penghasil enzim L-aparaginase dari sumber air panas Wawolesea dan untuk mengetahui aktivitas enzim L-asparaginase yang dihasilkannya. Bakteri termohalofilik penghasil enzim L-asparaginase diperoleh dengan tahapan: isolasi bakteri pada media NA yang mengandung NaCl 1,5% – 1,6%; seleksi bakteri penghasil L-asparaginase pada media M-9; produksi L-asparaginase dengan prinsip fermentasi pada media produksi serta pengukuran aktivitas dan aktivitas spesifik enzim L-asparaginase. Hasil isolasi menunjukkan adanya 14 isolat bakteri termohalofilik yang mampu menghasilkan enzim L-asparaginase. Aktivitas enzim L-asparaginase tertinggi yaitu 86,61 IU/mL pada isolat AAT3.2 dan terendah yaitu 38,24 IU/mL pada isolat CAT1.1. Aktivitas spesifik tertinggi 6767,98 IU/mg pada isolat CAT3.2 dan terendah 684,54 IU/mg pada isolat CAT1.1.Screening of L-asparaginase-Producing Thermohalophilic Bacteria from Wawolesea Hot Springs in Southeast Sulawesi and Their Enzyme Activity Test. The L-asparaginase is an enzyme that can convert L-asparagine to L-aspartate. L-asparagine can be utilized by leukemia cancer cells as a source of nutrition. The use of L-asparaginase on an industrial scale prioritizes L-asparaginase that exhibits optimal activity and stability at high temperatures due to the high reaction rate in hydrolyzing L-asparagine, stability against denaturants such as detergents and organic compounds, and stability under acidic or basic conditions. L-asparaginase with activity and stability at high temperatures can be explored from microorganisms that live in high-temperature and high-salinity environments. This study aimed to obtain isolates of thermohalophilic bacteria that produce L-asparaginase enzymes from Wawolesea hot springs and determine the activity of the L-asparaginase enzymes. Thermohalophilic bacteria producing L-asparaginase from Wawolesea hot springs were obtained by the following steps: isolation of bacteria on NA (Nutrient Agar) media containing 1.5% – 1.6% NaCl, selection of L-asparaginase-producing bacteria on M-9 media, production of L-asparaginase with the principle of fermentation on production media and measurement of activity and specific activity of L-asparaginase enzyme. The isolation results showed that there were 14 isolates of thermohalophilic bacteria capable of producing L-asparaginase. The highest L-asparaginase enzyme activity was 86.61 IU/mL in AAT3.2 isolates, and the lowest was 38.24 IU/mL in CAT1.1 isolates. The highest specific activity was 6767.98 IU/mg in isolate CAT3.2, and the lowest was 684.54 IU/mg in isolate CAT1.1.
Detection and Identification of Pathogenic Bacteria in Monomorium sp. Ant in The Environment of Kendari City Hospital, Southeast Sulawesi Amirullah, Amirullah; Yanti, Nur Arfa; Surachma, Wahyuni Nurul; Suriana, Suriana
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.41032

Abstract

Monomorium sp. ant is one of the insects that are considered pests in the hospital environment and potential to be mechanical vectors that can carry and spread pathogenic bacteria and cause nosocomial infections. This study aims to detect and identify pathogenic bacteria found in Monomorium sp. ants originating from the environment of Kendari City Hospital, Southeast Sulawesi. Sampling of Monomorium sp. was carried out by the bait/sugar trap method in 3 locations of Kendari City Hospital, namely internal inpatient rooms, nutrition installations, and laboratory installations. Detection of pathogenic bacteria was carried out by the pour plate method on selective media, namely MacConkey Agar and Mannitol Salt Agar. The identification of bacteria was carried out by numerical-phenetic analysis based on phenotypic characters using the MVSP 3.1 application. The results of the study identified 5 species of pathogenic bacteria found in Monomorium sp. ants from the Kendari City Hospital environment. Three species of bacteria were found in ants from the internal inpatient room, namely Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Brevundimonas vesicularis, and two species found in ants from nutrition installations, namely Pseudomonas luteola and Staphylococcus auricularis. No pathogenic bacteria were found in ants from the laboratory installation.
Inovasi Produk Olahan Pangan melalui Pemanfaatan Limbah Ampas Kelapa untuk Meningkatkan Pendapatan Masyarakat Kelurahan Talia, Kecamatan Abeli, Kota Kendari Ahmad, Sitti Wirdhana; Ardiansyah, Ardiansyah; Yanti, Nur Arfa; Ambardini, Sri; Muzuni, Muzuni; Amirullah, Amirullah; Prasetya, Wandy Murti
PUSAKA ABDIMAS Vol. 2 No. 1 (2025)
Publisher : Yayasan Serumpun Karang Konservasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61548/pa.v2i1.50

Abstract

ABSTRACT Coconut fruit processing produces by-products in the form of coconut pulp. Coconut pulp organic waste has a high fiber content that can benefit the body when consumed. So far, coconut pulp is only used as animal feed with very low product prices. The high amount of fiber content and the potential for protein and fat, provide opportunities for waste dregs to be developed into processed products. One of the efforts that can be done is to optimize the use of organic waste of coconut pulp into flour as raw material for making bread through community service activities. The problem faced by partners is the lack of information obtained by the community about making coconut pulp flour which can be used as a substitute for wheat flour for other food products.   The solution to this problem is to provide training and guidance on making coconut pulp flour and its processed products. This service aims to improve the skills of mothers by utilizing their potential and the potential of available natural resources optimally, in an effort to increase economic income. This training is the use of coconut pulp into flour for mothers in Talia Village, Abeli District, Kendari City. This method of service uses lectures, discussions and demonstrations. This training was attended by mothers in Talia Village, Abeli District. The results of the training obtained by the mothers of Talia Village can make bread from coconut pulp with various flavors. The training greatly supports the improvement of participants' skills and knowledge. During the training, all mothers enthusiastically participated in the course of activities and actively discussed both ways. Participants understand and can practice the technique of processing coconut pulp into raw materials for making bread and can be a business opportunity. Keywords: Coconut Dregs Waste, Coconut Dregs Flour, Bread, Kelurahan Talia   Abstrak Pengolahan buah kelapa menghasilkan produk samping berupa ampas kelapa. Limbah organik ampas kelapa memiliki kandungan serat yang tinggi yang dapat bermanfaat bagi tubuh apabila dikonsumsi. Selama ini ampas kelapa hanya dimanfaatkan sebagai pakan ternak dengan harga produk yang sangat rendah. Kandungan jumlah serat yang tinggi serta potensi protein dan lemak, memberi peluang limbah ampas untuk dikembangkan menjadi produk olahan. Salah satu upaya yang dapat dilakukan yaitu dengan mengoptimalkan pemanfaatan limbah organik ampas kelapa menjadi tepung sebagai bahan baku pembuatan roti melalui kegiatan pengabdian masyarakat. Permasalahan  yang  dihadapi  oleh  mitra  adalah  kurangnya  informasi  yang  didapatkan  oleh masyarakat tentang   pembuatan   tepung   ampas   kelapa   yang   dapat dimanfaatkan   sebagai   pengganti   tepung   terigu   untuk   produk   pangan   lainnya.   Solusi dari permasalahan tersebut adalah memberikan pelatihan dan bimbingan tentang pembuatan tepung ampas kelapa beserta produk olahannya. Pengabdian ini bertujuan meningkatan keterampilan ibu-ibu dengan memanfaatkan potensi yang dimiliki dan potensi SDA yang tersedia secara optimal, dalam upaya meningkatkan pendapatan ekonomi. Pelatihan ini merupakan pemanfaatan ampas kelapa menjadi tepung pada ibu ibu Kelurahan Talia Kecamatan Abeli Kota Kendari. Metode pengabdian ini menggunakan metode ceramah, diskusi dan demonstrasi. Pelatihan ini diikuti oleh ibu ibu di Kelurahan Talia Kecamatan Abeli. Hasil pelatihan diperoleh ibu-ibu Kelurahan Talia dapat membuat roti dari ampas kelapa dengan berbagai rasa. Pelatihan tersebut sangat mendukung peningkatan keterampilan dan pengetahuan peserta. Selama pelatihan, semua ibu-ibu antusias mengikuti jalannya kegiatan serta aktif berdiskusi dua arah. Peserta memahami dan dapat mempraktikkan teknik pengolahan ampas kelapa menjadi bahan baku pembuatan roti dan dapat menjadi peluang usaha. Kata Kunci: Limbah Ampas Kelapa, Tepung Ampas Kelapa, Roti, Kelurahan Talia
Pendampingan Program Pengembangan Edu-Ekowisata Mangrove Desa Tanjung Tiram Kabupaten Konawe Selatan Sulawesi Tenggara Jamili Jamili; Ida Usman; La Ode Kadidae; Suriana Suriana; La Ode Adi Parman Rudia; Nur Arfa Yanti
Jurnal Pengabdian UNDIKMA Vol. 4 No. 2 (2023): May
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v4i2.6553

Abstract

The service activity aims to provide an understanding to the youth organization regarding the potential of mangrove ecosystems to be developed as Edu-Ecotourism in Tanjung Tiram Village, North Moramo District, South Konawe Regency, Southeast Sulawesi. The method of implementing this service was carried out education, practice, and mentoring. A questionnaire and data analysis were carried out descriptively to evaluate the increase in understanding of youth organization. The results of this community service activity showed that the understanding of youth organizations in Tanjung Tiram village increased regarding the conservation of the mangrove ecosystem and its potential to be developed as an edu-ecotourism village and in practice-assistance activities, 11 types of mangrove ecosystem constituents were identified in Tanjung Tiram Village. With a good understanding of the community and the high diversity of mangroves, Tanjung Tiram Village could be developed into a mangrove Edu-Ecotourism Village.
Bakteri Asam Laktat dari Buah Mangga Arum Manis (Mangifera Indica L. Var. Arum Manis) Nur Arfa Yanti; Ardiansyah; Kurnia Yati
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lactic acid bacteria (LAB) are bacteria that are widely used in the food sector because they are safe for consumption. The aim of this study was to obtain lactic acid bacteria from the Arum sweet mango (Mangifera indica L. var. Arum sweet). Isolation of LAB was carried out by pour plate method using specific media de Man Rogosa Sharpe Agar (MRSA) + CaCO3. Characterization carried out included colony morphology, cell morphology, catalase test, and motility test. There were five isolates of lactic acid bacteria that were isolated from the mango fruit, namely BAL1.3, BAL2.1, BAL3.2, BAL4.2 and BAL5.2. The five LAB isolates had the same characteristics as the key LAB characters, namely bacillus (stem) cell shape, Gram positive, non-motile, no endospores and catalase negative. The five LAB isolates were indicated as members of the genus Lactobacilllus.
Co-Authors A. Nurhana Abdul Mun'im Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Alfin Alfin Amirullah -, Amirullah Andi Septiana Ardiansyah Ardiansyah Ardiansyah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Ardiansyah Ardiansyah Asmawati Munir, Asmawati Asrul Sani Aswan, Muhammad Aswan Budiman, Herdi Cahyanti, Kartika Dwi Dedy Oetama Desty Tryaswaty DIRVAMENA BOER, DIRVAMENA Erni Martani Fahyuddin Fahyuddin, Fahyuddin Fatimah Alwi Albakar Haidin Haidin Haji Muhiddin, Nurhayani Handayani Hamid, Fitri Ida Usman Ida Usman Indrawati Indrawati Irnawati Irnawati Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili Karya, Adi Kurnia Yati La Ode Adi Parman Rudia La Ode Adi Parman Rudia La Ode Ahmad Nur Ramadhan La Ode Iman Sulaiman La Ode Kadidae, La Ode Langkah Sembiring Lili Darlian, Lili M. Jahiding M. Rajab Sutra Mijaya Marlina, Wa Ode Leni Marwati Arji Mashuni Mashuni Mashuni Pallawagau Muhammad Jahiding Muhiddin, Nurhayani Haji Muhsin Muhsin Muhsin Muhsin Muhsin Muhsin Muksar Muksar Muzuni, Muzuni Nurhayani H. Muhiddin Nurhayani H. Muhiddin, Nurhayani H. Nurhayani Haji Muhiddin Prasetya, Wandy Murti Ramlawati, Ramlawati Santi Septiana, Santi Sebastian Margino Sebastian Margino Sitti Wirdhana Ahmad Sitti Wirdhana Ahmad Sri Ambardini Sri Ambardini Sri Noegrohati Sulaiman, La Ode Iman Suleman, Darwis Surachma, Wahyuni Nurul Suriana Suriana Suriana Suriana Suriana Suriana Syah, Muhamad Azwar Taufik Walhidayah Vidya Nur Riska Parakkasi Wa Ode Isra Wa Ode Nanang Trisna Dewi Wa Ode Sitti Nurhaliza Wahyu Ahwarul Asis Wina Rezky