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All Journal bionature AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati SEMIRATA 2015 BioWallacea Journal of Biological Research agriTECH Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL BIOLOGI INDONESIA BIOMA : Jurnal Biologi Makassar ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Pada Masyarakat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Biosaintifika: Journal of Biology & Biology Education Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Berkala Penelitian Agronomi Jurnal ABDINUS : Jurnal Pengabdian Nusantara IJOEP : International Journal of Ecophysiology Jurnal Pengabdian UNDIKMA Jurnal Mandala Pengabdian Masyarakat Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA Molekul: Jurnal Ilmiah Kimia Majalah Pengabdian Indonesia Mekongga: Jurnal Pengabdian Masyarakat Bionature
Nur Arfa Yanti
Jurusan Biologi FMIPA Universitas Halu Oleo, Kendari
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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BAKTERI ASAM LAKTAT DARI BUAH MANGGA ARUM MANIS (Mangifera indica L. var. Arum manis) Nur Arfa Yanti
bionature Vol 23, No 2 (2022): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i2.37860

Abstract

Bakteri asam laktat (BAL) merupakan bakteri yang banyak dimanfaatkan dalam bidang pangan karena aman dikonsumsi. Penelitian ini bertujuan untuk memperoleh bakteri asam laktat dari buah mangga Arum manis (Mangifera indica L. var. Arum manis). Isolasi BAL dilakukan dengan metode tuang (pour plate) mengunakan media spesifik de Man Rogosa Sharpe Agar (MRSA) + CaCO3. Karakterisasi yang dilakukan meliputi morfologi koloni, morfologi sel, uji katalase, dan uji motilitas. Bakteri asam laktat yang berhasil diisolasi  dari buah mangga Arum manis  sebanyak lima isolat yaitu isolat BAL1.3, BAL2.1, BAL3.2, BAL4.2 dan BAL5.2. Kelima isolat BAL memiliki karakter yang sama dengan karakter kunci BAL yaitu bentuk sel bacil (batang), bersifat Gram positif, non motil, tidak memiliki endospora  dan katalase negatif. Kelima isolate BAL diindikasikan sebagai anggota genus Lactobacilllus
EKSPLORASI BAKTERI TERMOHALOFILIK POTENSIAL PENGHASIL L-ASPARAGINASE SEBAGAI ANTIKANKER DI SUMBER AIR PANAS WAWOLESEA Jamaluddin Jamaluddin; Alfin Alfin; Muzuni Muzuni; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.59 KB) | DOI: 10.33772/biowallacea.v5i1.4591

Abstract

ABSTRAK Tujuan penelitian ini adalah untuk memperoleh isolat bakteri termohalofilik penghasil enzim L-aparaginase dari sumber air panas Wawolesea. Bakteri termohalofilik penghasil L-asparaginase asal sumber air panas Wawolesea diperoleh dengan tahapan: survey dan pengukuran parameter lingkungan sumber air panas Wawolesea; isolasi bakteri pada media NA (Nutrient Agar) dan seleksi bakteri penghasil enzim L-asparaginase pada media M-9. Hasil isolasi menunjukkan adanya 52 isolat bakteri termohalofilik dan 14 isolat diantaranya mampu menghasilkan L-asparaginase. Kata Kunci: Bakteri Termohalofilik, L-Asparaginase, Sumber Air Panas Wawolesea ABSTRACT  The objective of this study was to obtain isolates of thermohalophilic bacteria producing L-asparaginase enzyme from Wawolesea hot spring. L-asparaginase producing thermohalophilic bacteria from the Wawolesea hot spring are obtained by steps; survey and environmental parameters measurement of Wawolesea hot spring, isolation of bacteria on NA (Nutrient agar) medium and selection of L-asparaginase producing bacteria on M-9 medium. The isolation results showed 52 isolates of thermohalophilic bacteria and 14 isolates of them capable of producing L-asparaginase. Keywords: Thermohalophilic bacteria, L-asparaginase, Wawolesea hot spring
A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection Mashuni Mashuni; Nur Arfa Yanti; Irnawati Irnawati; Fitri Handayani Hamid; Muhammad Jahiding
Molekul Vol 18 No 1 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.1.6864

Abstract

Long-term accumulation of pesticides in the environment to human and animal health. Acetylcholinesterase (AChE) biosensors with highly sensitive potentiometer transducers based on the membranes of Ag, reduced graphene oxide (rGO), and chitosan (CS) has been successfully developed. The membrane was made with a composition of 0.5 mM AgNO3, 2.5 mg/mL rGO, and 2% (w/v) CS coated on the surface of the Au electrode. The composition of the membrane with three ratios, namely 1:1:2, 2:1:3, and 3:1:4. Then, membrane Ag/rGO/CS and the enzyme AChE were immobilized on the membrane surface. The prepared biosensor has excellent conductivity, catalytic activity, and biocompatibility attributed to the synergistic effect of Ag/rGO/CS and glutaraldehyde (GTA) as crosslinkers and providing a hydrophilic surface for AChE adhesion. The linear range in biosensors is 1 × 10-8 to 1 µg L-1 with a regression coefficient of 0.9803 for 1:1:2 membrane, 0.9836 for 2:1:3 membrane, and 0.9850 for 3:1:4 membrane. The LOD is about 1 × 10-7 µg L-1 for all membranes. In addition, the biosensor showed good sensitivity, acceptable reproducibility, and stability, having an RSD of less than 5%. This biosensor makes it possible to provide a new and promising tool for analyzing pesticides, especially organophosphates.
Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste Nur Arfa Yanti; Sitti Wirdhana Ahmad; Sri Ambardini; Nurhayani Haji Muhiddin; La Ode Iman Sulaiman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10241

Abstract

Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production. 
Aktivitas Antibakteri Kombucha Daun Sirsak (Annona muricata L.) Dengan Konsentrasi Gula Berbeda Yanti, Nur Arfa; Ambardini, Sri; Ardiansyah, Ardiansyah; Marlina, Wa Ode Leni; Cahyanti, Kartika Dwi
BERKALA SAINSTEK Vol 8 No 2 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i2.15968

Abstract

Kombucha daun sirsak merupakan minuman hasil fermentasi simbiosis antara bakteri asam asetat Acetobacter xylinum dan khamir Saccharomyces sp. yang menggunakan bahan baku rebusan daun sirsak. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kombucha daun sirsak dengan variasi konsentrasi gula yang berbeda. Konsentrasi gula yang digunakan dalam pembuatan kombucha daun sirsak adalah 10%, 20%, 30% dan 40% (b/v). Pengujian aktivitas antibakteri dilakukan dengan metode difusi sumuran menggunakan bakteri Escherichia coli dan Staphylococcus aureus. Kombucha daun sirsak memiliki aktivitas antibakteri dengan spektrum luas. Kombucha daun sirsak dengan konsentrasi gula 20% memiliki aktivitas antibakteri tertinggi. Dengan demikian, kombucha daun sirsak sangat berpotensi sebagai minuman kesehatan.
Training on Processing Natural Resources into Processed Food and Health Products for Mothers in Tondongggeu Village, Nambo District, Kendari City Ambardini, Sri; Ahmad, Sitti Wirdhana; Muhsin, Muhsin; Karya, Adi; Syah, Muhamad Azwar; Adi Parman Rudia, La Ode; Yanti, Nur Arfa
Majalah Pengabdian Indonesia Vol. 1 No. 3 (2024): Desember 2024
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v1i3.32

Abstract

This community service activity is a training activity on the manufacture of health and food products that have the opportunity to improve the community's economy as well as providing education on the use of moringa leaves as face masks and papaya fruit as an analog tomato sauce to mothers in Tondonggeu Village, Nambo District, Kendari City. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Papaya as a substitute for tomatoes in making analog tomato sauce can be done because papaya has characteristics like tomatoes, namely color, organic acid content, and viscosity, even papaya has a higher vitamin C level than tomatoes.  Moringa leaves contain many vitamins, including vitamin A, vitamin C, amino acids and antioxidants such as flavonoids, quercetin and zeatin which function to maintain dry skin hydration and prevent the appearance of wrinkles, because it helps fight free radicals, so it is very good to be used as a cosmetic ingredient in the form of a face mask. Participants in this training involved residents of Tondonggeu village, Thematic KKN students and a team of accompanying lecturers from the Biotechnology and Biology study program of FMIPA Halu Oleo University. The training methods used are lectures, demonstrations and discussions. The lecture method was carried out with the aim of providing information and knowledge about moringa masks and analog ketchup. The demonstration aims to provide skills to mothers through the practice of directly processing moringa leaves into masks and papaya fruits into analog tomato sauce while the discussion is intended to show the activeness and understanding of the community during the training process. The results of the training on making masks and analog tomato sauce carried out by mothers are in the good category with an average success of 85%, their response to the implementation of the training is very good as seen from the enthusiasm of the residents to participate in the activity from the beginning to the end. This training activity can increase knowledge and improve mothers' skills in producing moringa masks and papaya analogue tomato sauce.
Detection and Identification of Pathogenic Bacteria in Monomorium sp. Ant in The Environment of Kendari City Hospital, Southeast Sulawesi Amirullah, Amirullah; Yanti, Nur Arfa; Surachma, Wahyuni Nurul; Suriana, Suriana
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.41032

Abstract

Monomorium sp. ant is one of the insects that are considered pests in the hospital environment and potential to be mechanical vectors that can carry and spread pathogenic bacteria and cause nosocomial infections. This study aims to detect and identify pathogenic bacteria found in Monomorium sp. ants originating from the environment of Kendari City Hospital, Southeast Sulawesi. Sampling of Monomorium sp. was carried out by the bait/sugar trap method in 3 locations of Kendari City Hospital, namely internal inpatient rooms, nutrition installations, and laboratory installations. Detection of pathogenic bacteria was carried out by the pour plate method on selective media, namely MacConkey Agar and Mannitol Salt Agar. The identification of bacteria was carried out by numerical-phenetic analysis based on phenotypic characters using the MVSP 3.1 application. The results of the study identified 5 species of pathogenic bacteria found in Monomorium sp. ants from the Kendari City Hospital environment. Three species of bacteria were found in ants from the internal inpatient room, namely Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Brevundimonas vesicularis, and two species found in ants from nutrition installations, namely Pseudomonas luteola and Staphylococcus auricularis. No pathogenic bacteria were found in ants from the laboratory installation.
Pendampingan Program Pengembangan Edu-Ekowisata Mangrove Desa Tanjung Tiram Kabupaten Konawe Selatan Sulawesi Tenggara Jamili Jamili; Ida Usman; La Ode Kadidae; Suriana Suriana; La Ode Adi Parman Rudia; Nur Arfa Yanti
Jurnal Pengabdian UNDIKMA Vol. 4 No. 2 (2023): May
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v4i2.6553

Abstract

The service activity aims to provide an understanding to the youth organization regarding the potential of mangrove ecosystems to be developed as Edu-Ecotourism in Tanjung Tiram Village, North Moramo District, South Konawe Regency, Southeast Sulawesi. The method of implementing this service was carried out education, practice, and mentoring. A questionnaire and data analysis were carried out descriptively to evaluate the increase in understanding of youth organization. The results of this community service activity showed that the understanding of youth organizations in Tanjung Tiram village increased regarding the conservation of the mangrove ecosystem and its potential to be developed as an edu-ecotourism village and in practice-assistance activities, 11 types of mangrove ecosystem constituents were identified in Tanjung Tiram Village. With a good understanding of the community and the high diversity of mangroves, Tanjung Tiram Village could be developed into a mangrove Edu-Ecotourism Village.
Bakteri Asam Laktat dari Buah Mangga Arum Manis (Mangifera Indica L. Var. Arum Manis) Nur Arfa Yanti; Ardiansyah; Kurnia Yati
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lactic acid bacteria (LAB) are bacteria that are widely used in the food sector because they are safe for consumption. The aim of this study was to obtain lactic acid bacteria from the Arum sweet mango (Mangifera indica L. var. Arum sweet). Isolation of LAB was carried out by pour plate method using specific media de Man Rogosa Sharpe Agar (MRSA) + CaCO3. Characterization carried out included colony morphology, cell morphology, catalase test, and motility test. There were five isolates of lactic acid bacteria that were isolated from the mango fruit, namely BAL1.3, BAL2.1, BAL3.2, BAL4.2 and BAL5.2. The five LAB isolates had the same characteristics as the key LAB characters, namely bacillus (stem) cell shape, Gram positive, non-motile, no endospores and catalase negative. The five LAB isolates were indicated as members of the genus Lactobacilllus.
Detection and Identification of Pathogenic Bacteria in Monomorium sp. Ant in The Environment of Kendari City Hospital, Southeast Sulawesi Amirullah, Amirullah; Yanti, Nur Arfa; Surachma, Wahyuni Nurul; Suriana, Suriana
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.41032

Abstract

Monomorium sp. ant is one of the insects that are considered pests in the hospital environment and potential to be mechanical vectors that can carry and spread pathogenic bacteria and cause nosocomial infections. This study aims to detect and identify pathogenic bacteria found in Monomorium sp. ants originating from the environment of Kendari City Hospital, Southeast Sulawesi. Sampling of Monomorium sp. was carried out by the bait/sugar trap method in 3 locations of Kendari City Hospital, namely internal inpatient rooms, nutrition installations, and laboratory installations. Detection of pathogenic bacteria was carried out by the pour plate method on selective media, namely MacConkey Agar and Mannitol Salt Agar. The identification of bacteria was carried out by numerical-phenetic analysis based on phenotypic characters using the MVSP 3.1 application. The results of the study identified 5 species of pathogenic bacteria found in Monomorium sp. ants from the Kendari City Hospital environment. Three species of bacteria were found in ants from the internal inpatient room, namely Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Brevundimonas vesicularis, and two species found in ants from nutrition installations, namely Pseudomonas luteola and Staphylococcus auricularis. No pathogenic bacteria were found in ants from the laboratory installation.
Co-Authors A. Nurhana Abdul Mun'im Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Alfin Alfin Aminah Maryani Amirullah -, Amirullah Andi Septiana Andi Tenri Ampa Anto, Ananda Azzahra Ardiansyah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Asmawati Munir, Asmawati Asrul Sani Aswan, Muhammad Aswan Bakkareng, Wirdhana Budiman, Herdi Cahyanti, Kartika Dwi Dedi Oetama Desty Tryaswaty DIRVAMENA BOER, DIRVAMENA Erni Martani Fahyuddin Fahyuddin, Fahyuddin Haidin Haidin Haji Muhiddin, Nurhayani Handayani Hamid, Fitri Ida Usman IDA USMAN Ihtiar, Muhammad Indrawati Indrawati Irnawati Irnawati Irnawati Irnawati Jamaluddin Jamaluddin Jamili Jamili Karya, Adi Kurnia Yati La Ode Adi Parman Rudia La Ode Ahmad Nur Ramadhan La Ode Ahmad Nur Ramadhan La Ode Iman Sulaiman La Ode Kadidae, La Ode Langkah Sembiring Lili Darlian M. Jahiding M. Rajab Sutra Mijaya Marlina, Wa Ode Leni Marwati Arji Mashuni Mashuni Mashuni Pallawagau Muhammad Jahiding Muhiddin, Nurhayani Haji Muhsin Muhsin Muhsin Muhsin Muhsin Muhsin Muksar, Muksar Muzuni, Muzuni Nurhayani H. Muhiddin Nurhayani H. Muhiddin, Nurhayani H. Nurhayani Haji Muhiddin Putri, Kila Dayana Ramlawati, Ramlawati Reza Kurniawan Rudia, La Ode Adi Parman Santi Septiana, Santi Sebastian Margino Sebastian Margino Silondae, Kinaya Aghisna Y. Sitti Wirdhana Ahmad Sri Ambardini Sri Ambardini Sri Noegrohati Sulaiman, La Ode Iman Suleman, Darwis Surachma, Wahyuni Nurul Suriana Suriana Suriana Suriana Syah, Muhamad Azwar Taufik Walhidayah Vidya Nur Riska Parakkasi Wa Ode Isra Wa Ode Nanang Trisna Dewi Wa Ode Sitti Nurhaliza Wahyu Ahwarul Asis Wina Rezky