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ISOLASI, KARAKTERISASI, DAN UJI POTENSI BAKTERI PENGHASIL ENZIM TERMOSTABIL DARI SUMBER AIR PANAS LAINEA, SULAWESI TENGGARA Marwati Arji; Wina Rezky; Wa Ode Sitti Nurhaliza; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.59 KB) | DOI: 10.33772/biowallacea.v5i1.4592

Abstract

ABSTRAK Penelitian ini bertujuan untuk memperoleh isolat bakteri yang berpotensi sebagai penghasil enzim ekstraseluler “Proliase” (Protease, Lipase, Amilase dan Selulase) dan  mengetahui karakteristik isolat bakteri dari air panas Lainea. Tahapan-tahapan yang dilakukan dalam penelitian ini meliputi pengambilan sampel, isolasi bakteri, seleksi bakteri pada media spesifik serta karakterisasi bakteri potensial penghasil enzim ekstraseluler berdasarkan karakter fenotipik. Hasil penelitian ini diperoleh 36 isolat bakteri dan hanya terdapat 3 isolat bakteri yang memiliki potensi ganda dalam menghasilkan enzim ekstraseluler. Ketiga isolat bakteri memiliki bentuk sel batang, Gram positif, tidak menghasilkan endospora, bersifat motil, katalase positif, menunjukkan hasil negatif pada uji Indol, Methyl Red, Voges Proskauer, sitrat dan mampu memfermentasi sukrosa, glukosa dan mannitol, toleran terhadap konsentrasi NaCl 3-7%, pH 5-7 dan suhu 37-70oC. Ketiga isolat bakteri dari sumber air panas Lainea tersebut identik dengan genus acuan Listeria. Kata kunci : Air panas Lainea, Bakteri Proteolitik, Bakteri Lipolitik, Bakteri Amilolitik, Bakteri Selulolitik          ABSTRACT This study aims to obtain bacterial isolates that have the potential as producers of "Proliase" extracellular enzymes (Protease, Lipase, Amylase and Cellulase) and determine the characteristics of bacterial isolates from Lainea hot water. The stages carried out in this study include sampling, bacterial isolation, bacterial selection in specific media and characterization of extracellular enzyme-producing potential bacteria based on phenotypic characters. The results of this study obtained 36 bacterial isolates and there were only 3 bacterial isolates which had double potential in producing extracellular enzymes. The three bacterial isolates have the form of stem cells, Gram positive, do not produce endospores, are motile, positive catalase, show negative results on the test of Indol, Methyl Red, Voges Proskauer, citrate and are able to ferment sucrose, glucose and mannitol, tolerant to NaCl 3- concentration 7%, pH 5-7 and temperature of 37-70oC. The three bacterial isolates from Lainea's hot springs are identical to Listeria's reference genus. Keywords : Lainea Hot Water, Proteolytic Bacteria, Lipolytic Bacteria, Amylolytic Bacteria, Cellulolytic Bacteria
Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara M. Rajab Sutra Mijaya; Nur Arfa Yanti; Ardiansyah Ardiansyah; Nurhayani H. Muhiddin
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.347 KB) | DOI: 10.33772/biowallacea.v6i2.8825

Abstract

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar
Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Nur Arfa Yanti; Langkah Sembiring; Sebastian Margino
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB) | DOI: 10.22146/ijbiotech.7804

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A new bacterial strain that produces amylase and poly-a-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics  and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by  Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Nur Arfa Yanti; Langkah Sembiring; Sebastian Margino; Nurhayani H. Muhiddin
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.882 KB) | DOI: 10.22146/ijbiotech.7877

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB. Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Optimasi Produksi Poli-β-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10 Nur Arfa Yanti; Sebastian Margino; Langkah Sembiring
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2587

Abstract

A new strain characterized as Bacillus sp. PSA10 was found to produce poly-β-hydroxybutyrate (PHB) at concentration of 52.28% (g PHB/g dry cell weight) in shaken flask culture, using sago starch as a carbon source. This research is aimed to determine the optimum culture condition of PHB production Bacillus sp. PSA10 at laboratory scale. Optimization of PHB production was conducted in this research, in terms of inoculum concentration, concentration of the major components in minimal medium, environmental condition and incubation time. The result showed that optimum conditions for the production of PHB by Bacillus sp. PSA10 were achieved at minimal medium (Ramsay medium) with 5% (v/v) inoculum concentration, 2% (w/v) sago starch, 1.0 g/l (NH4)2SO4, 6.7 g/l Na2HPO4.7H2O, and 0 g/l KCl. The optimum environmental conditions were achieved with initial pH 7, temperature 37oC, agitation speed at 150 rotary per minute (rpm) and the best of incubation time was 48 hour. Under this optimum condition, the maximum PHB production by Bacillus sp. PSA10 increased from 52.28% to 71.35% (g PHB/g dry cell weight) at 48 hour cultivation. Therefore, Bacillus sp. PSA10 is potential to apply for PHB production from sago starch at industrial scale.
Acceleration of Paraquat Biodegradation by Isolated Soil Bacteria Erni Martani; Nur Arfa Yanti; Sebastian Margino; Sri Noegrohati
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 1 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10105

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Herbicides residues were reported to have impact on the ecosystem. It was thought that acceleration of paraquat degradation would minimize these impacts. Paraquat was persistent in peat soil due to low pH. This study was done to investigate the role of bacterial isolates on the acceleration of paraquat degradation, especially in peat soil. The bacteria were isolated from several kinds of Indonesian soils using enrichment culture technique in a modified N-free medium. The medium was added with paraquat at gradually increase-concentration from 10, 20, and 40 ppm (w/w). Examinations in paraquat degradation were done in two levels. The first was in a synthetic medium (N-free medium); the second was in soil extract medium. Two kinds of peat were used to make the soil extract media, i.e. fibric and saphric peat soils. Several bacterial isolates were able to degrade paraquat in N-free medium. However, the degradation mode was different with those in peat soil extract media. None of them degraded paraquat in fibric and saphric soil extract media. It was suggested that the environmental limiting factors were responsible to the failure of paraquat degradation. Two selected isolates were able to degrade paraquat when the pH value of the extract medium was enhanced to around 5.5. Bacterial isolate of SM1, which was isolated from acid sulfate soil of Central Kalimantan, was the best isolate which was able to degrade paraquat in synthetic medium and peat soil extract media, especially in fibric extract medium. It degraded around 30%of paraquat within 15 day. Experiments are being done to enhance paraquat degradation by inoculation of mixed cultures of selected bacterial isolates.
POTENSI LIMBAH CAIR TAHU SEBAGAI SUMBER NITROGEN PADA PRODUKSI SELULOSA BAKTERI Nur Arfa Yanti; Sri Ambardini; Wa Ode Isra; Vidya Nur Riska Parakkasi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 1 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i1.8804

Abstract

Produksi selulosa bakteri (nata) pada umumnya menggunakan pupuk ZA (Amonium sulfat) atau urea sebagai sumber nitrogen. Penggunaan sumber nitrogen alami seperti limbah cair tahu dalam produksi selulosa bakteri, merupakan alternatif pengganti pupuk ZA yang  lebih aman untuk dikonsumsi. Penelitian ini bertujuan untuk mengetahui potensi limbah cair tahu sebagai sumber nitrogen pada produksi selulosa bakteri. Produksi selulosa bakteri dilakukan menggunakan substrat air kelapa yang ditambahkan limbah cair tahu dengan perlakuan perbandingan antara air kelapa dan limbah cair tahu adalah 1 : 1; 1 : 3 dan 3: 1. Fermentasi selulosa bakteri dilakukan dengan metode statis (diam) selama 14 hari menggunakan bakteri Acetobacter xylinum. Parameter yang diukur meliputi ketebalan, rendemen, kadar serat kasar dan kadar air. Hasil penelitian menunjukkan bahwa selulosa bakteri dapat diproduksi pada perlakuan  perbandingan air kelapa dan limbah cair tahu 1 :1 dan 3 : 1, namun perlakuan substrat dengan perbandingan air kelapa dan limbah cair tahu 1 : 3 tidak menghasilkan selulosa bakteri. Selulosa bakteri yang diproduksi menggunakan subtrat air kelapa dan limbah cair tahu sebagai sumber nitrogen dengan  perbandingan 1 : 1 memiliki ketebalan 15,03 mm, rendemen 13,25%, kadar serat 0,29% dan kadar air 86,76%, sedangkan selulosa bakteri dari substrat dengan perbandingan air kelapa  dan limbah cair tahu 3 : 1 memiliki ketebalan 16,69 mm, rendemen  12,89%, kadar serat 0,28%, dan kadar air 87,11%. Dengan demikian, limbah cair tahu berpotensi digunakan sebagai sumber nitrogen untuk memproduksi selulosa bakteri. Kata kunci :Selulosa bakteri, Limbah cair tahu, sumber nitrogen, nata.
Characteristics of Biocellulose from Sago Liquid Waste with Different Ammonium Sulfate Concentration Nur Arfa Yanti
International Journal of Ecophysiology Vol. 1 No. 1 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (926.831 KB) | DOI: 10.32734/ijoep.v1i1.848

Abstract

This research aims to utilize sago liquid waste to produce biocellulose with different concentration of ammonium sulfate and to know characteristics of biocellulose. Production of biocellulose was done in sago liquid waste substrate with different concentration of ammonium sulfate, namely 0.5, 1, 1.5 and 2% (w/v) by using bacteria local strain Acetobacter xylinum LKN6. The observed parameters included thickness, yield, crude fiber and organoleptic.The analysis results showed that ammonium sulfate concentration given a different to the thickness, yield, crude fiber and organoleptic. The best characteristic of biocellulose obtained in the treatment of 1.5% ammonium sulfate concentration. The biocellulose from sago liquid waste potential as dietary fiber food.
Innovation in Scad Mackerel (Decapterus spp.) Processing Into Economically Valuable Products for Improving The Skills of Coastal Communities in Nambo Village Sitti Wirdhana Ahmad; Nur Arfa Yanti; Muhsin Muhsin; Andi Septiana; Muzuni Muzuni; Taufik Walhidayah
ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2022): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Muhammadiyah Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.919 KB) | DOI: 10.35568/abdimas.v5i1.1668

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Scad Mackerel (Decapterus spp.) is the main consumption commodity of coastal communities in Nambo village. Apart from public awareness is quite high, the price is affordable, and has nutritional value since unsaturated fats is low, so it good to consume for all ages. The public lack of knowledge about proper fish management. The solution is to provide an understanding of fish processing techniques so be more selling, delicious and nutritious, as well as for community empowerment with the aim of creating fisheries entrepreneurs. The method was the lecture method, socialization and training to the community as an empowerment effort to improve knowledge, skills and well-being. The implementation of the service in the form of location surveys, coordination with local regional chief of village and PKK administrators, preparation of equipment and materials, and some processed samples of fish, socialization about the benefits of fish and fish processing training
Penentuan Kandungan Fenolik Total Liquid Volatile Matter dari Pirolisis Kulit Buah Kakao dan Uji Aktivitas Antifungi terhadap Fusarium oxysporum Mashuni Pallawagau; Nur Arfa Yanti; M. Jahiding; La Ode Kadidae; Wahyu Ahwarul Asis; Fitri Handayani Hamid
ALCHEMY Jurnal Penelitian Kimia Vol 15, No 1 (2019): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.36 KB) | DOI: 10.20961/alchemy.15.1.24678.165-176

Abstract

Kulit buah kakao (KBK) adalah limbah organik dari hasil olahan buah kakao yang dapat digunakan sebagai sumber senyawa aktif. Kulit kakao mengandung senyawa selulosa, hemiselulosa dan lignin yang dapat terdekomposisi menghasilkan senyawa fenolik. Penelitian ini bertujuan untuk menentukan kandungan fenolik total atau Total Phenolic Content (TPC) dari liquid volatile matter (LVM) hasil pirolisis KBK dan uji aktivitas antifungi terhadap Fusarium oxysporum. Produksi LVM dihasilkan dari metode pirolisis KBK pada suhu 500 °C. Penentuan TPC dengan metode spektrofotometri UV-Vis menggunakan pereaksi Folin- Ciocalteu (FC) pada panjang gelombang maksimum 765 nm dengan larutan standar asam galat dan diukur setelah dibiarkan selama 60 menit pada suhu kamar. Uji aktivitas antifungi terhadap F. oxysporum dilakukan dengan metode dilusi. LVM dari hasil pirolisis KBK adalah 25,80% (b/b) dengan TPC 100% sebesar 2,28 g/L. Pirolisis KBK menghasilkan LVM yang memperlihatkan aktivitas antifungi dengan konsentrasi hambat minimal (KHM) 1% (v/v) dan konsentrasi bunuh minimal (KBM) 9% (v/v). Oleh karena itu, LVM KBK memiliki potensi untuk diaplikasikan sebagai fungisida alami.Determination of The Total Phenolic Content of Liquid Volatile Matter from Cocoa Pod Husk Pyrolysis and Antifungal Activity Test Against Fusarium oxysporum. The cocoa pod husk (CPH) is an organic waste from the processed cocoa potentially used as source of active compounds. The CPH contains cellulose, hemicelluloses, and lignin compounds that can be decomposed into phenolic compounds. The present study investigated the determination of total phenolic content (TPC) of liquid volatile matter (LVM) of CPH followed by the antifungal activity test against Fusarium oxysporum. The LVM production was performed by the pyrolysis method at temperature of 500 °C. The determination of TPC was evaluated by the UV-Vis spectrophotometric method using the Folin-Ciocalteu (FC) reagent at maximum wavelength of 765 nm with the standart solution of gallic acid measured after 60 min reaction at room temperature. The antifungal activity test against F. oxysporum was carried out by dilution method. The pyrolysis of CPH produces LVM of 25.80% (b/b) contained TPC 100% of 2.28 g/L. The LVM produced in this study shows antifungal activity with a minimal inhibitory concentration (MIC) 1% (v/v) and minimum fungicidal concentration (MFC) 9% (v/v). Therefore, the LVM CPH produced can be applied as a natural fungicide.
Co-Authors A. Nurhana Abdul Mun'im Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Alfin Alfin Amirullah -, Amirullah Andi Septiana Ardiansyah Ardiansyah Ardiansyah Ardiansyah Ardiansyah Ardiansyah Ardiansyah ARDIANSYAH ARDIANSYAH Asmawati Munir, Asmawati Asrul Sani Aswan, Muhammad Aswan Budiman, Herdi Cahyanti, Kartika Dwi Dedy Oetama Desty Tryaswaty DIRVAMENA BOER, DIRVAMENA Erni Martani Fahyuddin Fahyuddin, Fahyuddin Fatimah Alwi Albakar Haidin Haidin Haji Muhiddin, Nurhayani Handayani Hamid, Fitri Ida Usman Ida Usman Indrawati Indrawati Irnawati Irnawati Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili Karya, Adi Kurnia Yati La Ode Adi Parman Rudia La Ode Adi Parman Rudia La Ode Ahmad Nur Ramadhan La Ode Iman Sulaiman La Ode Kadidae, La Ode Langkah Sembiring Lili Darlian, Lili M. Jahiding M. Rajab Sutra Mijaya Marlina, Wa Ode Leni Marwati Arji Mashuni Mashuni Mashuni Pallawagau Muhammad Jahiding Muhiddin, Nurhayani Haji Muhsin Muhsin Muhsin Muhsin Muhsin Muhsin Muksar Muksar Muzuni, Muzuni Nurhayani H. Muhiddin Nurhayani H. Muhiddin, Nurhayani H. Nurhayani Haji Muhiddin Prasetya, Wandy Murti Ramlawati, Ramlawati Santi Septiana, Santi Sebastian Margino Sebastian Margino Sitti Wirdhana Ahmad Sitti Wirdhana Ahmad Sri Ambardini Sri Ambardini Sri Noegrohati Sulaiman, La Ode Iman Suleman, Darwis Surachma, Wahyuni Nurul Suriana Suriana Suriana Suriana Suriana Suriana Syah, Muhamad Azwar Taufik Walhidayah Vidya Nur Riska Parakkasi Wa Ode Isra Wa Ode Nanang Trisna Dewi Wa Ode Sitti Nurhaliza Wahyu Ahwarul Asis Wina Rezky