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Journal : Rumphius Pattimura Biological Journal

DNA Barcoding Application Using CYTB (Cytochrome-B) Marks for Authentication of Patin Fish (Pangasius sp.) Products in Surabaya Area Aulia, Wina Intan Nur; Setiawan, Edwin; Triwibowo, Radestya; Saputro, Triono Bagus; Hidayati, Dewi; Nurhayati, Awik Puji Dyah; Ridho, Muhammad Ainur Rosyid
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p079-092

Abstract

DNA barcoding is a practical and accurate molecular technique for identifying processed fish products that lack morphological characteristics. However, its application is limited by the availability of reference databases, DNA quality, and primer suitability. This study aims to determine the genetic identity of pangasius catfish (Pangasius sp.) marketed in Surabaya using the Cytochrome b (CytB) gene and to evaluate PCR-RFLP as an alternative to sequencing. A total of twenty samples (fresh and frozen fillets) were analyzed through DNA extraction and PCR amplification of the CytB gene, followed by in silico and in vitro analyses.The results show that CytB demonstrates high amplification success and provides sufficient resolution for species-level identification based on Neighbor-Joining analysis with the Kimura 2-parameter model. The study confirms that the samples were genetically identified as Pangasius pangasius and Pangasius hypophthalmus. This research highlights the novelty of integrating in silico prediction with laboratory validation to improve marker evaluation in DNA barcoding studies. Furthermore, it demonstrates that CytB-based PCR-RFLP is a rapid and cost-effective alternative to sequencing for detecting species mislabeling. Overall, the CytB gene is proven to be a reliable molecular marker for species authentication, supporting traceability systems and strengthening seafood authenticity monitoring in commercial markets.
Co-Authors Adik Mayang Indiani Adik Mayang Indiani Afrilya Dina Salsabila Agus Wahyudi Agus Wahyudi Alfani Raziful Dwi Ersandy Alifia Cahyaning Putri Anak Agung Gede Sugianthara Anjar Tri Wibowo Annisya Fitri Rachmada Ary Bachtiar Krishna Putra Aulia Istighfarotunnisa Aulia, Wina Intan Nur Bagastyo, Arseto Yekti BUDI SETIADI DARYONO Danang Samudra Devi Pramesti Wulandari Dewi Hidayati Didit Prasetyo Dini Adni Navastara, Dini Adni Dini Ermavitalini Edwin Setiawan Elshinta Riantica Firli Rahmah Primula Dewi Fitri Lianingsih Geoloveriana Esasi Hapsari, Lukiteswari Dyah Tri IDAA Warmadewanthi Istriyati Istriyati Kamila Islamiati Kharisma Armida Ulayya Kristianto Kristianto Kusuma, Baskara Wiku Adi Kuswytasari, Nengah Dwianita Latifah, Vida Rahma Lissa Rosdiana Lissa Rosdiana Maitsa Adzro Fania Mamuaya, Brigita Klara Krisdina Meitha, Karlia Miftahudin . Millah, Ro’iqotul Nadhila, Auliya Rahma Nadila Wulan Cahyani Natanael Hari Wijaya Ni Wayan Ni Wayan Nichole J de Voogt Noor Nailis Sa'adah Noor Nailis Saadah Noor Nailis Saa’dah Noor Nailis Sa’adah Nova Maulidina Ashari Nova Maulidina Ashuri Nugroho, Giri Nurlita Abdulgani Nurlita Abdulgani Nurul Jadid Putro, Karso Suryo Rahmadani, Syakirah Shafwah Rarastoeti Prastiwi Rarastoeti Pratiwi Ratna Rintaningrum riantica, elshinta Ridho, Muhammad Ainur Rosyid Rony Irawanto Sa'adah, Noor Nailis Saptarini, Dian Sari, Noorkomala Sartika, Dian Sa’adah, Noor Nailis Shabrina Syifa Ghaissani Shabrina Syifa Ghaissani Shovitri, Maya Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Subagus Wahyuono Sukardiman Sukma, Nur Awaliyah Mentari TATIK CHIKMAWATI Triono Bagus Saputro, Triono Bagus Triwibowo, Radestya Welly Herumurti Wijayanti Pujitono Yusuf, Adib Fakhruddin Zufar Azzaria Azmi Zuwanita, Cyntya Sri