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All Journal HAYATI Journal of Biosciences Jurnal Biotek Medisiana Indonesia The Indonesian Biomedical Journal Universa Medicina Argipa (Arsip gizi dan Pangan) Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Acta Biochimica Indonesiana Jurnal Ilmu Kefarmasian Indonesia Jurnal Kesehatan Bioscientia Medicina : Journal of Biomedicine and Translational Research Majalah Kesehatan Pharmamedika Bioscientia Medicina : Journal of Biomedicine and Translational Research Indonesian Archives of Biomedical Research (InABR) Makara Journal of Science Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Ani Retno Prijanti
Departemen Biokimia Dan Biomolekuler Fakultas Kedokteran Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Cytoglobin expression in rat kidney during exposure to systemic chronic hypoxia Ika Superti Daruningrum; Ani Retno Prijanti; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.55

Abstract

Background: The kidneys in physiological conditions are always in a state of relative hypoxia. Cytoglobin (Cygb) is the newest globin protein found of the globin family. One of the functions of Cygb is in oxygen supply. Cygb expression is found to increase in hypoxic conditions, which are thought to be an adaptation response to hypoxia. Objective: This study aimed to analyze the expression of Cygb in rat kidneys which were exposed to chronic systemic hypoxia. Methods: Twenty five male Sprague-Dawley rats, weighing 150-200 g were used in this experiment. Rats were divided into 5 groups: The control group was exposed to normoxia; the hypoxia groups (10% oxygen / 90% nitrogen) for 1 day; 3 days; 7 days and 14 days. After treatment, rats were sacrificed and their kidneys were taken. Cygb mRNA expression was measured by qRT-PCR, while Cygb protein expression was measured by the ELISA method. Results: The expressions of Cygb mRNA and protein were found to be highest on day 3 of hypoxia and was correlated very strongly and significantly (r2 = 0.96; p <0.05). Conclusion: The highest expression of Cygb on day 3 of chronic systemic hypoxia exposure is suggested as an attempt to restore oxygen supply to the kidneys.
Correlation between malondialdehyde level and FOXO3 and CASP3 mRNA expression changed in early-onset preeclampsia placenta Ni Made Wiasty Sukanty; Febriana Catur Iswanti; Syarifah Dewi; Muhammad Faruqi; Alyssa Shafa Andiana; Ani Retno Prijanti
Acta Biochimica Indonesiana Vol. 4 No. 2 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.61

Abstract

Background: Preeclampsia is one of the factors causing the high maternal mortality rate. The risk of morbidity and mortality is higher in Early Onset Preeclampsia (EOPE). Failure of spiral artery remodeling can cause oxidative stress that can inhibit placental development and increase trophoblast apoptosis. Objective: This study aims to analyze the oxidative stress and apoptosis of EOPE placentas. Methods: This study is an observational study with a cross-sectional design. A total of 31 EOPE placentas and 31 normal term placentas were used to measure the concentration of malondialdehyde (MDA) and the relative mRNA expression of FOXO3 and CASP3 using the spectrophotometric and RT-qPCR methods. Results: There was no difference in MDA concentration (p = 0.580) and FOXO3 (p = 0.467) and CASP3 (p = 0.243) mRNA expression in the normal and EOPE groups. There was a strong positive correlation between FOXO3 and CASP3 mRNA expression in the normal (p= 0.0001; r = 0.938) and EOPE groups (p = 0.0001; r = 0.855). There was no correlation between MDA concentration to FOXO3 (p = 0.124; r = 0.282) and CASP3 (p = 0.569; r = 0.106) mRNA expression in normal placenta. There was positive correlation between MDA concentration to FOXO3 (p = 0.016; r = 0.429) and CASP3 mRNA expression in EOPE placenta (p = 0.028; r = 0.395). Conclusion: These results indicate that cell integrity is still maintained through the autophagy process and the level of apoptosis in the EOPE placenta is regulated by ROS through FOXO3.
Control of HIF-1α Levels Potentially Promotes the Tissue Repair in Various Conditions Through Target Gene Expression Riyadh Firdaus; Ani Retno Prijanti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 1 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v6i1.429

Abstract

Hypoxia inducible factor-1 (HIF-1) is a transcription factor that plays an important role in maintaining oxygen balance at both the cellular and systemic levels, and is associated with various controls in the body. HIF-1 is a heterodimer of alpha and beta subunits. Alpha subunits are mostly dependent on oxygen levels in the body. In many cancers, excessive HIF-1α is thought to be involved in the promotion of tumor growth and metastasis. In addition, in the induction of systemic hypoxia, there is an increase of HIF-1α in the heart, brain, and even the kidneys as an adaptation response to hypoxia. Several studies regarding HIF-1a expression in traumatic brain injury, found that HIF-1a increased immediately after TBI, and decreased significantly after 24 hours. This can be used as a basis for further research on HIF-1a control as an effort to stop tissue damage or even help tissue repair.
Penghambatan Ekspresi Gen dengan Antisense Oligonukleotida sebagai Upaya Pengobatan Penyakit Dian Ratih Laksmitawati; Ani Retno Prijanti
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2084.271 KB)

Abstract

Due to the development of biomolecular science eg. overexpression genes, genes that cause diseases can be identfied, Based on this fact, researchers developed a therapeutic strategy by inhibiting the gene expression using oligonucleotide antisense. Therapy using oligonucleotide antisense was based on a natural process of gene expression. Specific artificial antisense will match complementary with DNA and mRNA. By this process the transcription will stop. The effort of therapy is relatively new but a few have been carried out in the clinical trial phase. Obstacles are encountered in reaching the target cell by the oligonucleotide antisense.
PENGARUH SUPLEMENTASI SERAT PSYLLIUM HUSK DAN DIET RENDAH KALORI SEIMBANG TERHADAP KADAR APO B PENYANDANG OBES I Rozana Nurftri Yulia; Victor Tambunan; Ani Retno Prijanti
ARGIPA (Arsip Gizi dan Pangan) Vol 1 No 2 (2016)
Publisher : UHAMKA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.402 KB)

Abstract

ABSTRACTGlobal raising of obesity prevalence nowday had influence to increasing morbidity andmortality cardiovascular disease (CVD). One of obesity implication is incresing of aterogeniclipid profles such as low-density lipoprotein (LDL). This lipoprotein contains apolipoproteinB (apo B) as hydrophilic agent in circulation. Researches show that level of apo B was betterpredictor of CVD risk factor. The most effective technique in weight loss such as llifestylesinterventions for obese is combining diet and exercise. Current advice is to increase the amountof dietary fber up to 20-35 g/day, for more effective weight loss management. Dietary fber hassignifcant characteristics that affect physiological and metabolic roles including its solubilityin water. Psyllium husk (PH) is a source of natural soluble fber obtained from plantago ovataForssk seed. The aim of this study to investigate the change of apo B level in obese I aftergiven supplementation psyllium husk (PH) 8.4 g/day and low-calorie balanced diet (LCBD)for 4 weeks. We conducted a double blind randomized clinical trial with parallel design.Subjects were randomly allocated into two groups; treatment (T) group and plasebo (P) group.The T subjects received psyllium husk (PH) 8.4 g/day and LCBD 1200 kcal/day and the Psubjects received placebo and LCBD 1200 kcal/day. Data collected in this study consist ofsubject distribution and characteristic, intake of energy, macronutrient, fber, water and apoB level that assessed before and after treatment. Level of statistical analyses signifcance was5%, independent t-test and Mann-Whitney. A total 28 subjects (14 subjects in each group)had completed the intervention. There were no serious adverse events were reported along theintervention. Mean of age in T and P groups respectively was 35.0 (30.0-45.0) years and 34.5(30.0-48.0) years, and BMI was 28.0 ± 1.1kg/m2 and 27.2 ± 1.4 kg/m2. The energy intake in Tgroup 1130.9 ± 221.9 kcal/day was signifcantly higher (p = 0.02) than P group 1024.3 ± 269.9kcal/day. Simple carbohydrate intake in T group (35,6 (8.3-69.9) g/day) was signifcantlyhigher (p<0.0001) than in P group (13.8 (3.4-55.5) g/day). Intake of dietary fber in T groupwas 17.2 ± 2.8 g/day had signifcantly higher than P group 8.2(5.2-15.2) g/day, even addingPH supplementation cannot meet the recommendation of fber intake (20-35 g/day). Decreasingof apo B level in P group was -6.1 ± 8.9 mg/dL that statistically insignifcant difference (p =0.13) with T group -1.3 ± 7.3 mg/dL. We concluded PH supplementation 8.4 g/day and LCBD1200 kcal/day in obese I can not reduce apo B level in 4 weeks.
The role and regulation of FOXO1 in carbohydrate metabolism and its targeting in metabolic diseases Ani Retno Prijanti; Ni Made Wiasty Sukanty
Indonesian Archives and Biomedical Sciences Vol 1 No 2 (2021): Indonesian Archives of Biomedical Research (InABR). 1(2): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.708 KB) | DOI: 10.55392/indarcbiores.v1i2.8

Abstract

Carbohydrate is the first energy source used in metabolic processes. Failure in carbohydrate metabolism can cause metabolic diseases, and many studies are focused on its therapy. Until now, there is no specific therapy approved. Despite that, one of the main alternatives is regarding the involvement of FOXO1 protein in metabolic disease progress. As a transcription factor for gluconeogenesis genes, FOXO1 can increase blood glucose levels. It also involves various signaling pathways in carbohydrate metabolisms such as PI3K/Akt and PKA in which many proteins act as FOXO1 regulators through posttranslational modification. The vital role of FOXO1 in carbohydrate metabolism provides an opportunity to make FOXO1 the main target of metabolic disease therapy. Various proteins and natural compounds can either directly or indirectly regulate FOXO1 activity. It can be an option to be used to control blood glucose levels by targeting FOXO1
Spirulina platensis effect on oxidative stress of rat’s offspring brain exposed to cigarette smoke during pregnancy and lactation Kenny Cantika Abadi; Febriana Catur Iswanti; Sri Widia A Jusman; Fadilah Fadilah; Ani Retno Prijanti
Acta Biochimica Indonesiana Vol. 5 No. 1 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.64

Abstract

Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for the fetus. The smoke contains many free radicals that could be eliminated by antioxidant. This study aimed to investigate the effect of Spirulina platensis ethanol extract as antioxidant against cigarette smoke exposure during pregnancy until lactation by assessing oxidative stress markers in neonatal brain tissues. Methods: The experimental study used 26 offspring divided into four groups: (C) = offspring of maternal control group; (Cg) = offspring of maternal exposed to cigarette smoke; (CgSp) = offspring of maternal given spirulina and exposed to cigarette smoke; and (Sp) = offspring of maternal given spirulina only group, during gestation and 9 days lactation (30 days). Each group consisted of 6 offspring obtained from 2 adult females mated with male Sprague-Dawley rats. The exposure of cigarette smoke was 4 burn cigarettes/day for 30 days. The dose of extract was 200 mg/kg BW. The offspring were sacrificed, and the brain tissues were taken for MDA, MnSOD activity, as well as catalase activity, carbonyl, and GSH. Results: There was no significant differences in MDA level between groups. The carbonyl, SOD, and catalase activity did not differ between the control and smoked group. Conclusion: Exposure of four burned cigarettes smoke per day during pregnancy, and 9 days of lactation did not trigger oxidative stress. However, the effect of Spirulina platensis administration on rat offspring brain could not be analyzed.
Modulation of the NF-κB Activation Pathway by Phycocyanobilin from Spirulina platensis: An in Silico Study Iswanti, Febriana Catur; Purba, Hastuti Handayani S; Prijanti, Ani Retno; Fadilah, Fadilah; Herlina, Linda; Paramita, Reni
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several studies have predicted the molecular interactions of the active ingredient of Spirulina platensis as an anti-inflammatory compound. However, these interaction studies did not review the modulation of the NF-κB activation pathway, which involves various factors. This study demonstrated the potential of the bioactive compounds of S. platensis for modulating immune function by reducing inflammation through the inhibition of the NF-κB activation pathway. Phycocyanobilin was predicted to have good potential for molecular docking with multisubunit IκB kinase (IKK)1/IKKA, IKK2/IKKB, NF-κB-inducing kinase, and the IκBα/NF-κB complex. Furthermore, β-carotene exhibited good potential for interactions with NF-κB essential modulator/IKK and the NF-κB complex, and α-glucan had the potential for interactions with COX-2. Therefore, supplementation with S. platensisand its bioactive compounds is expected to provide optimal benefits. Several studies have predicted the molecular interactions of the active ingredient of Spirulina platensis as an anti-inflammatory compound. However, these interaction studies did not review the modulation of the NF-κB activation pathway, which involves various factors. This study demonstrated the potential of the bioactive compounds of S. platensis for modulating immune function by reducing inflammation through the inhibition of the NF-κB activation pathway. Phycocyanobilin was predicted to have good potential for molecular docking with multisubunit IκB kinase (IKK)1/IKKA, IKK2/IKKB, NF-κB-inducing kinase, and the IκBα/NF-κB complex. Furthermore, β-carotene exhibited good potential for interactions with NF-κB essential modulator/IKK and the NF-κB complex, and α-glucan had the potential for interactions with COX-2. Therefore, supplementation with S. platensis and its bioactive compounds is expected to provide optimal benefits.
Supplementation Impact of Spirulina platensis Ethanol Extract on Inflammatory Homeostasis Modulation of Rat Spleen at Different Ages Paramita, Reni; Purba, Hastuti Handayani S; Prijanti, Ani Retno; Iswanti, Febriana Catur
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1231-1242

Abstract

Pro- and anti-inflammatory mediators need to be released in a balanced way to maintain a healthy state as we age. One important regulatory element in the equilibrium of pro- and anti-inflammatory mediators is NF-κB. The purpose of this study was to examine how S. platensis affected the control of inflammatory mediators in young, healthy, emerging adults and adults in rats. In this investigation, 200 mg/kg BW of S. platensis extract was administered to six groups of male Wistar rats, ages 12, 18, and 24 weeks, along with a control group. In both the treatment and control groups, NF-κB p65 protein expression was lower at 24 weeks than it was at 12 and 18 weeks. TNF-α and COX-2 proteins were lower in the treatment group than in the control group. All age groups in the treatment group had higher levels of IL-10 protein than the control group. The quantity of NF-κB p65 was positively correlated with COX-2 and TNF-α. By raising the concentration of NF-κB p65, the ethanolic extract of S. platensis altered a mediator of cellular immunity. A decrease followed this in TNF-α and COX-2 and a rise in IL-10 in the rat spleen at different ages.
Significance Increased of Xanthine Oxidase Activity in Placenta with Pregnancy Complications of Preeclampsia and Gestational Diabetes Compared to Normal Novientri, Gissi; Prijanti, Ani Retno
Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education Research Institute Vol. 6 No. - (2022): Proceedings Book of International Conference and Exhibition on The Indonesian M
Publisher : Writing Center IMERI FMUI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69951/proceedingsbookoficeonimeri.v6i-.132

Abstract

Certain risky complications in pregnancy are due to failure of pseudo-vasculogenesis of arteria spiralis, which leads to inadequate nutrition, hypoxia, and inflammation. These can be caused by eating disorders, stress, or other risk factors. Preeclampsia (PE) and Gestational Diabetes (GD) are pregnancy complications that are currently of concern. In Indonesia, the prevalence of PE reaches 3-10%, with a maternal mortality rate of 50%. Meanwhile, the prevalence of GD ranges from 1.9-3.6%, and half of GD patients are diagnosed with type 2 Diabetes Mellitus (DM) after 5-10 years of giving birth. Oxidative stress arises as a result of those pregnancy complications. Various studies have also been conducted to determine the pathways that produce reactive oxygen species (ROS), the leading causes of oxidative stress. Xanthine oxidase (XO) is an enzyme that contributes to ROS production. Uniquely this enzyme is the converted form of xanthine dehydrogenase (XDH), which plays a minimal role in ROS production. The conversion of XDH to XO significantly increased in PE and GD cases, characterized by increased XO activity in maternal and fetal circulation and placental tissue compared to normal pregnancies. This paper will describe the conversion mechanism of XDH to XO in PE and GD conditions and compare its enzyme activity to normal pregnancy.
Co-Authors AA Hawali Adiyarianni, Ghina Alyssa Shafa Andiana Andriani Andriani Andrijono Andrijono Andrijono Andrijono Anggraeni, Tricia Dewi Bambang Sutrisna Dalilati, Annia Zhafarina Desak Gede Budi Krisnamurti Dian Ratih Laksmitawati Dicky Levenus Tahapary Dinutanayo, Wimba Widagdo Fadilah Fadilah Fadilah Fadilah, Fadilah Febriana Catur Iswanti Ferry Sandra Fitriyadi Kusuma Frans D Suyatna Frans Ferdinal H R Helmi Hariyono Winarto Heri Wibowo Hikmat, Ujang Saeful Ika Superti Daruningrum Inawati Wanandi, Septelia Jusman, Sri Widia Azraki Jusman, Sri Widia Azraki Kencana, Faradiyan Kenny Cantika Abadi Laila Nuranna Liem, Isabella Kurnia Linda Herlina Luh Putu Ratna Sundari M. Rafi Malano, Yoarva Mohamad Sadikin Mohamad Sadikin Mohamad Sadikin Muhamad Arif Budiman Muhammad Faruqi Ni Made Wiasty Sukanty Ninik Mudjihartini Novientri, Gissi Purba, Hastuti Handayani S Purwosunu, Yuditiya Puwosunu, Yuditiya Reni Paramita Riyadh Firdaus Rizka Ramadhani Rozana Nurftri Yulia Sadikin, Abdul Halim Sari, Adinda Juwita Sri Mutya Sekarutami Sri Widia A Jusman Syarifah Dewi Tjam Diana Samara Tri Handayani Utami, Tofan Widya Victor Tambunan Wawan Mulyawan Werdhasari, Asri Yurika Sandra