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Journal : Jurnal Veteriner

Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.
Identifikasi dan Karakterisasi Bakteri Asam Laktat Isolat Susu Segar Sapi Bali (IDENTIFICATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BALI CATTLE’S RAW MILK) I Nengah Sujaya; Komang Ayu Nocianitri; Ni Putu Desy Aryantini; Wayan Nursini; Yan Ramona; Yoshitake Orikasa; Fukuda Kenji; Tadashu Urashima; Yuji Oda
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle is an indigenous spesies in Bali, which pay great attention due to its uniqueness. Numerousarticles have been published on Bali cattle especially related to its disease, nutritional requirement forgrowth and domestication. Nevertherless, it was no any report has been published on the lactic acidbacteria (LAB) assosiated with the cattles raw milk and its potential used as probiotic. This work isaimed to identify LAB isolated from bali cattle raw milk and its resistance to secondary bile acid (sodiumdeoxy cholic), a prequisite in development of probiotic for human. The results revealed that based upon thehomology studies of the variable region I, II, and III sequences of the 16S rDNA showed that 44 out of 62isolates were closely related to Pediococcus acidilactici; 11 out of 62 isolats were closely related to Enterococusgallinarum, five out of 62 isolates were closely related to Lactococcus garvieae, while only one isolate was closely related to Lactobacillus plantarum and Weisella confusa. Some isolates showed resistant to 0.2-0.6mM deoxy cholic acid, which might be also resist in human gastrointestinal tract conditions. Based onthose finding, it can be concluded that the LAB associated with raw bali cattle milk were closey related toP. acidilactici, E. gallinarum, Lac. garvieae, Lb. plantarum and W. confusa, which different from thosecommonly LAB found in others cattle raw milk. Somes isolates were potential to be developed as probioticfrom human helath.
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM SUMBAWA MARE MILK Nengah Sujaya; Yan Ramona; Ni Putu Widarini; Ni Putu Suariani; Ni Made Utama Dwipayanti; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to isolate and characterize lactic acid bacteria (LAB) from the Sumbawa mares milk The Isolation of LAB was conducted in Man Rogosa Sharpe (MRS) agar. The isolates were characterized by standard methods, such as Gram staining, cell morphology study and fermentation activities. The ability of the isolates to inhibit some pathogenic bacteria was studied by dual culture assay. Isolates showing the widest spectrum of inhibiting pathogenic bacteria were further identified using API 50 CHL. The results showed that Sumbawa mare milk was dominated by lactobacilli and weisella/leuconostoc. As many as 26 out 36 isolates belong to homofermentative lactobacilli and another 10 isolates belong to both heterofermentative lactobacilli and weissella or leuconostoc. Twenty four isolates inhibited the growth of Escherichia coli 25922, Shigela flexneri, Salmonella typhimurium, and Staphylococcus aureus 29213. Two promising isolates with the widest spectrum of inhibiting pathogenic bacteria, Lactobacillus sp. SKG34 and Lactobacillus sp. SKG49, were identified respectively as Lactobacillus rhamnosus SKG34 and Lactobacillus ramnosus SKG49. These two isolates were specific strains of the sumbawa mare milk and are very potential to be developed as probiotic for human.
PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK I Nengah Sujaya; I Made Utami Dwipayanti; Ni Luh Putu Suariani; Ni Putu Widarini; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this capability is not appropriate for probiotic. This character is worthly to be studied since it has never been reported in lactobacilli.
Co-Authors ., Komalawati Agung Yogeswara, Ida Bagus Aimatun Nisfia Rizqy Aldy Wulang Kambombu Alfredo Umbu Londong Anak Agung Ayu Kanaka Mutiara Laksmi Ari Indra Dewi, Ni Nyoman Ate, Olyn Tien Ayu Wita Kusumawati, I Gusti Carolina Widyawati Christimulia Purnama Trimurti Cok. Istri Dewiyani Cakrawati Damayanti, Ni Made Rai Dewa Ayu Amira Budinitri Dewi, Ni Nyoman Ari Indra Djelantik, Sang Ayu Made Agung Prasetiawati Dylla Hanggaeni Dyah Puspaningrum Ela Tara Wini Bira Eunike Grace Br. Sinambela Fransiska Sisilia Bheni Fukuda Kenji Ginting, Maria Fransiska I Gede Mustika I Gede Mustika I Gede Mustika I Gede Putu Sedana Putra I Gusti Agung Ayu Dewi Landari I Gusti Ayu Wita Kusumawati I Gusti Kusumawati I Ketut Agung Sudewa I Komang Krisna Praskadewa I Made Wisnu Adhi Putra I Nengah Sujaya I Putu Krisna Indrayana I Wayan Surya Aryasa Adiprawira Ida Bagus Agung Yogeswara Ida Bagus Ketut Mantra Ida Bagus Mantra Indra Tirta Saman Jeany Ingnadea Sabuin Jessica Putri Lulu Budiarsa Jiwantara, Gusti Ngurah Oka Ketut Ayu Widiantini KOMANG AYU NOCIANITRI Krisantus Markus Dede Kurniati , Ni Made Luh Putu Melly Purnama Dewi Luh Suariani Luh Suariani, Luh Mantra, Ida Bagus Ketut Marcianus Raya Goa Maria Adensiani Dola Bili Maria Reinaldis Jebaut Subin Mertayasa, I Gede Agus NI KADEK YUNITA SARI Ni Ketut Wiradnyani Ni Luh Made Ema Juliasari Ni Luh Okta Ayuni Ni Luh Putu Suariani Ni Luh Utari Sumadewi Ni Made Ayu Suardani Singapurwa Ni Made Kurniati Ni Made Utami Dwipayanti Ni Putu Desy Aryantini Ni Putu Eny Sulistyadewi Ni Putu Suariani Ni Putu Widarini Ni Wayan Deswiniyanti Noviana Bili Petronela Yuyun Kurniawati Purwaningtyas Kusumaningsih Purwaningtyas Purwaningtyas Putri, Syadila Nugraheny Muliya PUTU INDAH LESTARI Putu Wida Gunawan Rafly Arisanto Rai Riska Resty Wasita Siti Zamiatun Zarah Sri Endah K, Natalia Sudewa, I Ketut Agung Sulatri, Ni Luh Sumadewi, Ni Luh Utari Tadashu Urashima Viani, Agata Winduyasa, I Wayan Y. Ayu Fitria Adi Pravitasari Yan Ramona Yoanita Theresia Sina Yogeswara, Ida Bagus Yoshitake Orikasa Yuji Oda