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Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Mubarakati, Nurul Jadid; Aulanni'am, Aulanni'am; Sumitro, Sutiman Bambang; Ciptadi, Gatot
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Nurul Jadid Mubarakati; Aulanni'am Aulanni'am; Sutiman Bambang Sumitro; Gatot Ciptadi
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Co-Authors Abdul Hamid A. Toha Abdullah Rozzen Muzayyan Adi Sofyan Ansori, Muhammad Agus Mohammad Hikam Ahmad Taufiq Alik Maulidiyah Annisatul Anis Hakimah Ari Hayati Arifiati, Shinta Arina Roikhana Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Azis Mawardi Azis Mawardi Chintya Ayu Pertiwi Della Aljanna Savita Devi Indrayani Fitri Asari Dewi Qurrot 'Aini Dewi, Atikah Nur Shabrina Razan Dyan Aprillia Susanti Eka Kumalasari Fradina, IntanTrixi Gatot Ciptadi Gatra Ervi Jayanti Gatra Ervi Jayanti Hakimah, Annisatul Anis Hasan Zayadi Hatif Khusnin Nida' Hikam, Agus Mohammad Husain Latuconsina Islami, Darajatun Islamya, Nur Afni Auli Ismatud Diniyah Istirochah Pujiwati Januarista, Terra Kamilah, Nahdhiyatun Kharisma, Viol Dhea Kurniasari, Yulia Lestari, Tengku Anggun M. Abdul Qodir Jailani Ma'ruf, Muhammad Maghfiroh, Faldha Laili Malia Anjani Moch Mustakim Moch Mustakim Muchammad Faruq Vikriansyah Muhammad Dailami, Muhammad Musaffak, Taufik Ridwan Nabilah Miftachul Khoiriyah Nafisa Nafisa Nia Novita Nurrohmah Nour Athiroh Abdoes Sjakoer Nur Cahya Kartika Slamet Nur Laily Azizah Nur Mufida Nur Mufida Nurrohmah, Nia Novita Nurul Humaidah Oktavia Rahayu Puspitarini Oktaviana, Nimas Ajeng Pinanti, Honesty Nurizza Pratiwi, Radita Intan Aisyah Radita Intan Aisyah Pratiwi Rahayu Dwi Lestari Ramadhan, Majida Ramizard Rafsanjani Rasyadan Taufiq Probojati Rossa Martha Wulandari Rossa Martha Wulandari Saimul Laili Sama’ Iradat Tito Saputri, Syntia Diyah Hayu Ria Silvia, Putri Azizatul Siti Khoirun Ni’mah Siti Qurrotul Aini Sulis Setiawati Suroyya, Mariyam Sutiman B. Sumitro Sutiman Bambang Sumitro Syaiful Arifin Tengku Anggun Lestari Thiya Fathiyatul Fauziyah Tintrim Rahayu, Tintrim Ula, Erfa Navadiatul Umu Intan Kinasih Vina Fitria Wulandari Waliyyul Ahdi Alhikami Widyananda, Muhammad Hermawan Yunani, Silvi