Article Per Year (5 Year)
p-Index From 2020 - 2025
5.746
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Indonesian Journal of Agricultural Science INDONESIAN JOURNAL OF PHARMACY Indonesian Journal of Chemistry JFIOnline Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Majalah Info Sains Journal of Pharmaceutical and Sciences.
Wilmar Maarisit
Universitas Kristen Indonesia Tomohon
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

Published : 61 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 61 Documents
Search

 1   2   3   4   5 
TOXIC ACTIVITIES OF HEXANE EXTRACT AND COLUMN CHROMATOGRAPHY FRACTIONS OF RODENT TUBER PLANT (Typhonium flagelliforme Lodd.) ON Artemia salina Sianipar, Nesti F.; Maarisit, Wilmar; Valencia, Alice
Indonesian Journal of Agricultural Science Vol 14, No 1 (2013): April 2013
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is a medicinal plant  particularly found in Java. The plant is used as an ingredient for  conventional cancer treatment. The aim of this study was to determine the toxic activities of crude extracts and column chromatography fractions of  rodent tuber on Artemia salina larvae. Rodent tuber plant was obtained  from the Indonesian Spice and Medicinal Crops Research Institute in  Bogor, West Java. The experiment was conducted in the Biology  Laboratory of Universitas Pelita Harapan, Tangerang, Banten. Leaves and petioles of the plant were macerated with acetone and the filtrates were evaporated (40°C) to obtain crude extracts. The crude extracts were partitioned with ethyl acetate, followed with hexane, chloroform and  butanol. Toxicity test of the extracts was performed using the Brine Shrimp Lethality Test (BSLT) method on A. salina larvae. Extract showing the most toxic was fractioned using column chromatography and then tested on the larvae. The experiment was designed in a completely randomized  factorial, four replicates for crude extracts and two replicates for the fractions. Treatments were different types of extracts (hexane, chloroform and butanol) at various concentrations (500, 1,000 and 1,500 μg ml-1 of 5% Tween solution). Fractions of the column chromatography used were taken from the column number 1, 3 and 10, and tested their toxicities at 200, 400, 600, 800 and 1,000 μg ml-1 of 5% Tween solution. Parameters observed were the death of A. salina expressed as LC50. The study  showed that hexane extract of the petioles had the most toxic to A. salina (LC50 = 762.08 μg ml-1). Fraction number 10 showed the highest toxic (LC50 = 381.07 μg ml-1), whereas the lowest was fraction number 3 (LC50 = 653.13 μg ml-1). The study indicates that rodent tuber plant from Bogor is toxic to A. salina and further test for its cytotoxic activity is justified.
Aktivitas Antibakteri dan Antimitotik dari Fungi yang Bersimbiosis dengan Spons Maarisit, Wilmar; Minelko, Marstella; Jan, Tan Tjie
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.295 KB)

Abstract

Sponge-derived fungi are source of natural bioactive compounds. The aim of this research is to test the anti-bacterial and anti-mitotic activities from marine sponge-derived fungus. The sponge-derived fungus (strain TID.24041021-2) has been successfully isolated from sponge (strain TID.24041021-2) collected from the sea around Tidung Island. The morphology of this fungus has been identified and classified in the genus Wangiella sp. This fungus was cultivated in PDA with salinity 25 ppm for 21 days at room temperature, extracted with acetone, partitioned with ethyl acetate, and tested the anti-bacterial activity against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus, and also tested the anti-mitotic activity using root tip cells of Allium cepat. The results showed that crude extract of ethyl acetate could inhibit the growth of B. subtilis 91.30-2.30 mm), E.coli (1.50-3.03 mm) dan S.aureus (2.80-5.0 mm) bacteria. Also, antimitotic activity from crude extract of ethyl acetate showed that there is chromosome abnormalities of 0.76 and 1.38 in extract concentration of 20% and 25%, respectively.Keywords : Sponge-derived fungi, anti-bacterial, anti-mitotic, chromosome abnormalities.
Aktivitas Antibakteri dan Antimitotik dari Fungi yang Bersimbiosis dengan Spons Maarisit, Wilmar; Minelko, Marstella; Jan, Tan Tjie
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.295 KB) | DOI: 10.35617/jfi.v6i2.130

Abstract

Sponge-derived fungi are source of natural bioactive compounds. The aim of this research is to test the anti-bacterial and anti-mitotic activities from marine sponge-derived fungus. The sponge-derived fungus (strain TID.24041021-2) has been successfully isolated from sponge (strain TID.24041021-2) collected from the sea around Tidung Island. The morphology of this fungus has been identified and classified in the genus Wangiella sp. This fungus was cultivated in PDA with salinity 25 ppm for 21 days at room temperature, extracted with acetone, partitioned with ethyl acetate, and tested the anti-bacterial activity against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus, and also tested the anti-mitotic activity using root tip cells of Allium cepat. The results showed that crude extract of ethyl acetate could inhibit the growth of B. subtilis 91.30-2.30 mm), E.coli (1.50-3.03 mm) dan S.aureus (2.80-5.0 mm) bacteria. Also, antimitotic activity from crude extract of ethyl acetate showed that there is chromosome abnormalities of 0.76 and 1.38 in extract concentration of 20% and 25%, respectively.Keywords : Sponge-derived fungi, anti-bacterial, anti-mitotic, chromosome abnormalities.
The Effects of Gamma Irradiation on Growth Response of Rodent Tuber (Typhonium flagelliforme Lodd.) Mutant in In Vitro Culture NESTI FRONIKA SIANIPAR; ARIANDANA WANTHO; . RUSTIKAWATI; WILMAR MAARISIT
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.697 KB) | DOI: 10.4308/hjb.20.2.51

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is an Indonesian native plant that potential as anticancer. Even though the potential use of bioactive compounds from T. flagelliforme is very high, its genetic variation in Indonesia is low. Gamma irradiation can be used to increase genetic variation. The objective of this research was to obtain the first generation mutants (M1) of T. flagelliforme resulting from gamma irradiation of normal mother plantlets with different plant height and number of shoots. T. flagelliforme was irradiated at the doses of 0, 10, 20, 30, 40, and 50 Gy. The percentage of plant death was then calculated to determine the Lethal Dose (LD) 50. T. flagelliforme was then re-irradiated at LD50 dose to obtain M1 mutant. The plant height and number of shoots variation was observed after eight weeks of culture. The analysis of T. flagelliforme radiosensitivity resulted in LD50 at 25 Gy. Observation at six weeks after irradiation showed significant differences between the normal mother plant, 20 Gy mutants, and 25 Gy mutants with an average plant height of 9.57, 3.41, and 2.43 cm respectively, and the  average number of shoots produced was 7.85, 6.03, and 5.00 shoots respectively. Irradiation at 20 and 25 Gy produced 49 and 37 mutant plantlets. This research showed that gamma irradiation at LD50 dose could generate plant height and number of shoots variation of M1 mutant plantlets that are different from normal mother plantlets.
TOXIC ACTIVITIES OF HEXANE EXTRACT AND COLUMN CHROMATOGRAPHY FRACTIONS OF RODENT TUBER PLANT (Typhonium flagelliforme Lodd.) ON Artemia salina Nesti F. Sianipar; Wilmar Maarisit; Alice Valencia
Indonesian Journal of Agricultural Science Vol 14, No 1 (2013): April 2013
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v14n1.2013.p1-6

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is a medicinal plant  particularly found in Java. The plant is used as an ingredient for  conventional cancer treatment. The aim of this study was to determine the toxic activities of crude extracts and column chromatography fractions of  rodent tuber on Artemia salina larvae. Rodent tuber plant was obtained  from the Indonesian Spice and Medicinal Crops Research Institute in  Bogor, West Java. The experiment was conducted in the Biology  Laboratory of Universitas Pelita Harapan, Tangerang, Banten. Leaves and petioles of the plant were macerated with acetone and the filtrates were evaporated (40°C) to obtain crude extracts. The crude extracts were partitioned with ethyl acetate, followed with hexane, chloroform and  butanol. Toxicity test of the extracts was performed using the Brine Shrimp Lethality Test (BSLT) method on A. salina larvae. Extract showing the most toxic was fractioned using column chromatography and then tested on the larvae. The experiment was designed in a completely randomized  factorial, four replicates for crude extracts and two replicates for the fractions. Treatments were different types of extracts (hexane, chloroform and butanol) at various concentrations (500, 1,000 and 1,500 μg ml-1 of 5% Tween solution). Fractions of the column chromatography used were taken from the column number 1, 3 and 10, and tested their toxicities at 200, 400, 600, 800 and 1,000 μg ml-1 of 5% Tween solution. Parameters observed were the death of A. salina expressed as LC50. The study  showed that hexane extract of the petioles had the most toxic to A. salina (LC50 = 762.08 μg ml-1). Fraction number 10 showed the highest toxic (LC50 = 381.07 μg ml-1), whereas the lowest was fraction number 3 (LC50 = 653.13 μg ml-1). The study indicates that rodent tuber plant from Bogor is toxic to A. salina and further test for its cytotoxic activity is justified.
ANTIMICROBIAL METABOLITES FROM A MARINE-DERIVED FUNGUS Wilmar Maarisit; Katsuhiro Ueda
Indonesian Journal of Pharmacy Vol 24 No 3, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.513 KB) | DOI: 10.14499/indonesianjpharm0iss0pp163-169

Abstract

A marine fungus was separated from the surface of a marine red algae. Ethyl acetate extract of the cultivated marine fungus inhibited the growth of the pathogenic bacteria E.coli and S.aureus, and the phytopathogenic fungus C. cucurbitarum. Separation of the EtOAc extract gave four compounds, which were identified as new anthraquinonederivatives 3 and 4, and the known chrysophanol (1) and rubelin A (2) by the extensive analysis of NMR data. Compounds 1-4 inhibited the growth of the gram negative bacteria E. coli and gram positive bacteria S. aureus. Compound 3 also inhibited the fungus C. cucurbitarum.Key words: marine-derived fungus, structure elucidation, anthraquinone, antimicrobial activity
ANTIANGIOGENESIS AND ANTIBACTERIAL ACTIVITIES FROM AN INDONESIAN MARINE-DERIVED FUNGUS Dactylaria sp Wilmar Maarisit; Dicki Huga Tangiono; Reinhard Pinontoan; Marstella Minelko; Joke Luis Tombuku; Tan Tjie Jan
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (639.561 KB) | DOI: 10.14499/indonesianjpharm24iss2pp100-106

Abstract

Marine-derived fungi have been proven to be rich sources of chemically diverse natural products with a broad range of biological activities. The aim of this study was to determine the antibacterial and antiangiogenesis activities of marine derived fungi Dactylaria sp. Cultivation of the fungus Dactylaria sp (strain TID 24041021-1) was isolated from the marine invertebrate sponge. Culture of marine fungus was macerated with acetone and partitioned with ethyl acetate. The ethyl acetate extracts with 50, 100, and 200µg/mL concentrations, were assayed for their antiangiogenic activity by using chorioallantoic membrane in vivo. At the same time, ethyl acetate extracts at levels of 5, 10, 15, and 20mg/mL were assayed to pathogenic bacteria Bacillus subtilis, Escherichia coli, and Staphylococcus aureus using well diffusion method. The result of this study showed that ethyl acetate extract at concentration 50µg/mL could inhibit angiogenesis. The best antiangiogenic activity was showed at concentration of 200µg/mL ethyl acetate extract. Antibacterial activity from ethyl acetate extract inhibited the growth of B. subtilis (2.25-5mm), E. coli (0.63-3.50mm) and S. aureus (01.75mm) bacteria. Key words: Marine sponge-derived fungi, antiangiogenesis activity, antibacterial activity
UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN KEJI BELING Stobilanthes crispa (L.) Blume PADA TIKUS PUTIH Rattus norvegicus YANG DIINDUKSI ALOKSAN Ferdinand Palit; Gideon Tiwow; Wilmar Maarisit; Einstein Karundeng; Ferdy Karauwan
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 1 No. 1 (2018): Oktober 2018
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.42 KB) | DOI: 10.55724/jbiofartrop.v1i1.27

Abstract

Penelitian ini merupakan penelitian eksperimental yang terdiri dari lima perlakuan, masing-masingperlakuan terdiri dari tiga ekor hewan uji sebagai ulangan. Perlakuan I kontrol negatif aquadest,Perlakuan II kontrol positif metformin, Perlakuan III ekstrak etanol daun keji beling 75 mg/kg BB,Perlakuan IV ekstrak etanol daun keji beling 150 mg/kg BB, Perlakuan V ekstrak etanol daun kejibeling 300 mg/kg BB. Hasil penelitian menunjukkan pemberian ekstrak etanol daun keji belingmampu menurunkan kadar gula darah, yaitu dosis 75 mg/kg BB dengan nilai rata-rata penurunan14%, dosis 150 mg/kgBB dengan nilai 19.33% dan dosis 300 mg/kgBB dengan nilai 31.66%.Kesimpulannya adalah ekstrak etanol daun keji beling memiliki aktivitas antidiabetes.
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN BANDOTAN Ageratum conyzoides L SEBAGAI HEMOSTATIS TERHADAP LUKA POTONG PADA TIKUS PUTIH. Rivaldy Lobot; Olvie Datu; Wilmar Maarisit; Silvana Tumbel
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 1 No. 1 (2018): Oktober 2018
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.451 KB) | DOI: 10.55724/jbiofartrop.v1i1.30

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol daun bandotan (Ageratumconyzoides L) sebagai hemostatis terhadap luka potong pada tikus putih. Metode penelitian yangdigunakan adalah metode Rancangan Acak Lengkap (RAL), dengan lima kelompok perlakuandengan tiga kali ulangan. Kelompok pertama yaitu kelompok tanpa perlakuan, kelompok kedua,pemberian ekstrak daun bandotan konsentrasi 50%, kelompok ketiga, pemberian ekstrak daunbandotan konsentrasi 75%, kelompok keempat, pemberian ekstrak daun bandotan konsentrasi 100%,kelompok kelima, kontol positif betadin povidone iodine 10%. Data dianalisis menggunakan metodeANOVA (Analysis of variant) dengan tingkat kepercayaan 95% (?=0,05). Dan dilanjutkan denganuji LSD (Least Significant Different) untuk melihat perlakuan mana yang menberikan efek yangberbeda. Hasil penelitian terdapat perbedaan waktu berhentinya pendarahan dari setiap perlakuan.Ekstrak daun bandotan memiliki efektivitas sebagai hemostatis terbaik pada konsentrasi 75%
Uji Efektivitas Ekstrak Etanol Daun Awar-awar Ficus septica Burm F. Sebagai Antipiretik Terhadap Tikus Putih Rattus novergicus Glaudia Tawi; Wilmar Maarisit; Olvie Datu; Yessie Lengkey
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 2 No. 1 (2019): April 2019
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.094 KB) | DOI: 10.55724/jbiofartrop.v2i1.32

Abstract

Tumbuhan herbal mempunyai potensi senyawa bioaktif sebagai antipiretik. Penelitian ini bertujuanuntuk membuktikan bahwa daun awar awar Ficus septica dengan konsentrasi 10%, 20% dan 40%memiliki efektivitas antipiretik dengan penginduksi demam pepton 5%. Pengukuran suhu rektal tikusmenggunakan thermometer. Penelitian ini bersifat eksperimental laboratorium yang menggunakanhewan uji tikus putih. Hewan uji dibagi menjadi lima kelompok yaitu kelompok Kontrol negatif(aquades), kontrol postif (suspensi parasetamol) dan kelompok uji yaitu pemberian ekstrak daunawar-awar 10%, 20% dan 40%. Data yang diperoleh dianalisis dengan menggunakan uji anova danuji tukey. Berdasarkan hasil penelitian yang dilakukan, maka dapat disimpulkan bahwa ekstrak daunawar-awar Ficus septica konsentrasi 40% memiliki efektivitas sebagai antipiretik yang lebih efektifdalam penurunan suhu.
Co-Authors . RUSTIKAWATI Aditia Biasa Alice Valencia Alice Valencia Amal Ginting Amal Ginting Ana Neman Anti, Reski ARIANDANA WANTHO Asmiati Mulkin Asmuruf, Yunice Besin, Cecilia S. M. Carolina, Saroinsong F. Christin, Andriany M. Citra Mentari Payow Debora Christy Palit Debora J.A Tumbel Deby Mongi dengen, Alvina maria Deo Tampongangoy Desi E. Gusungi Desmita Muada Dicki Huga Tangiono Douglas N. Pareta Effie Karlin Nathania Einstein Karundeng Einstein Z. Karundeng Einstein Z. Z. S. Karundeng Esterlina J. Watung Febrilia Mangirang Ferdinand Palit Frengky Mawea Friska Mery Montolalu Gideon A. R. Tiwow Ginting, Margaretha S. Glaudia Tawi Glorya Sevani Pongoh Greti M. Kurama Hanna M. Rumagit, Hanna M. Hariyadi Hariyadi Haryadi Haryadi Indriani Maitulung Iteke Tuda Jabes Wolter Kanter Jeane Mongi Jeivia Matialo Jesica N. Bawondes Julianti Julianti Kalalo, Michael R. C. Kalalo, Reine Kalangi, Only Imando Karauwan, Ferdy Katsuhiro Ueda Kojongian, Cliveno Kristian Stefano Pinatik Lengkey, Yessie Maarisit, Abigail F. Marsela Pauner Marshella Mokoagouw Marstella Minelko Marstella Minelko, Marstella Meisy Permata Citra Nayoan Misran Lawani Nerni Potolangi Nesti F. Sianipar Nesti F. Sianipar NESTI FRONIKA SIANIPAR Olvie Datu Olvie S. Datu Paat, Vlagia Indira Pakingki, Priska Pangkei, Octry M. Pareta, Douglas Natan Patrick Rambe Pelealu, Nancy C. Priska Juanita Pakingki Rahmat S. Santoso Rallia Binuni Randi Tampa’i Rawung, Intan J. F. Reinhard Pinontoan Rivaldy Lobot Rondonuwu, Tesalonika Royke R. Palandi Rudy Pareda Sambou, Christel N. Sarah Sambow Sheren J. Bermula Silvana L. Tumbel Simanjuntak, Alfared S. Sonny Untu Sumagando, Adolfina Supit, Arter Tampa'i, Randy Tan Tjie Jan, Tan Tjie Tenci Lie Sarbunan Tombuku, Joke Luis Tulandi, Selvana Stien Veronica Davis Yapi Saroinsong Yappi Saroinsong Yosna Yundu Yuliana Polopadang Yunita Syawal Yusuf Tapehe