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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia Jurnal Pengendalian Hayati Berkala Ilmiah Pertanian Jurnal Sain Peternakan Indonesia JURNAL PETERNAKAN JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Caraka Tani: Journal of Sustainable Agriculture Warta Pengabdian PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Tropical Wetland Journal Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia INTEGRITAS : Jurnal Pengabdian Jurnal Abdidas Jurnal Sains dan Teknologi Peternakan Jurnal Proteksi Tanaman Tropis Journal La Lifesci Jumat Pertanian: Jurnal Pengabdian Masyarakat AGARICUS: Advances Agriculture Science & Farming KARYA: Jurnal Pengabdian Kepada Masyarakat Inovasi Jurnal Pengabdian Masyarakat (IJPM) PAPUMA: Journal of Comunnity Services Jurnal Agripet IPSSJ
Sigit PRASTOWO
Fakultas Pertanian, Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Jurnal Agripet

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Pengaruh Keberadaan Corpus Luteum terhadap Kualitas dan Tingkat Maturasi Oosit Domba Lokal Umur Pubertas Awal Secara In Vitro Widyastuti, Rini; Syamsunarno, Mas Rizky Anggun Adipurna; Yusuf, Alvin; Ridhlo, Muhammad Rosyid; Prastowo, Sigit
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i2.12103

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 (4 lapis), grade 2 (34 lapis) dan grade 3 (02 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro.(Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty)ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by 4, 34, and 02 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production program
Co-Authors . Jihan . Jihan . Suharto Adi Ratriyanto Agung Sih Kurnianto Agung, Sih Al Musa, Akmalus Salam Alfarisy, Fariz Kustiawan Ali Wafa Ambar Susanti Ananta, Rizqi Candra Yuda Anggara, Yudistira Dwi Ani Toshiba Anindya Salsabilla Ankardiansyah Pandu Pradana ARI SUSILOWATI Arsanti, Titania Artamevia, Adella Vadia Aryahya, Mayisya Najmuts Zahra Aryo Fajar Sunartomo Ati Kusmiati Ayunda, Berliana Dwi Bagaskara, Septian Tri Cahyo Basuki, Basuki Chandra, Vegarico Dani Kurniawan, Dani Darusalam, Zulfan Riski Distiana Wulanjari Fadilla Rahma Aulia Fahrani, Dinda Inggar Farok, Ahfas Syahdan Anwar Fathurrohmah Putri Dzikriyah Fawnia, Antesa Farah Fermansyah, Mahatir Muhammad Febi Gabrillo, Christina A. Hadian, Gloria Rida Handayani, Nurika Hari PURNOMO Hasjim, Saifuddin Hoesain, Mohammad Hutapea, Fredella Ibanah, Indah Ika Sumantri Indah Kurniasari Khansa Bashira Pondavi Kusumaningrum, Ayu Kusumawati, Ati Laily Dwi Dzulhijja Laily Dwi Dzulhijja Lanjar Sumarno Lenny Widjayanthi Mas Rizky A.A. Syamsunarno Maulidyanu, Firdaus Putra Mawla, Ega Fazle Moh. Wildan DJADMIKO Mubarok, Ananda Nur Ichwan Muhammad Hoesain MUHAMMAD RIZAL Mukarromah, Dilayatul Mustaqiem, Muhammad Muwahidi, Amirul Nafis, Muhammad Nisa, Nadia Novitasari, Erliana Dwi Nugraheni, Dita Fauziah Nurlita Pratiwi Pambuko, Galih Pradiar Ikhsanu Pradista, Luthfi Adya Pratama, Andhika Surya Pratama, Yoga Surya Purwa Desriyandi Purwandarini, Anggi Arsy Putra, Muhammad Agustin Akhirul Putri, Azzahra Purwo Sugito Rachmi Masnilah Rahayu Kusumaningrum Rasidi, Ahmad Haris Ridhlo, Muhammad Rosyid Rini Widyastuti Rosi, Annisa Salsabila S, Setiyono Saviera, Shavya Sarah Shohab MABDUH Sitio, Raffly Pandapotan Suharto Suharto Sutarno Sutarno Teni Wahyuningsih Tri Candra Setiawati Tri Satya Mastuti Widi Tristianto Nugroho Ulfa, Laila Badriyah Vanessa, Rebecca Verdaningsih, Yasmin Wachju Subchan Wagiyana Wagiyana Wagiyana Wagiyana, Wagiyana Widi, TriSatya Mastuti Widyas, Nuzul Widyas, Nuzul Wulanjani, Distiana Wulanjari, Distiana Yagus Wijayanto Yusuf, Alvin Zahra, Ayu Fatimatus Zikri, Fahrul