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Journal : BERITA BIOLOGI

REGENERASI TANAMAN KERKLILY {Lilium longiflorum Thunb.) MELALUI EMBRIOGENESIS SOMATIK PADA EKSPLAN DAUN Priyono Priyono
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1125

Abstract

The effects of plant growth regulators on somatic embryo genesis in Lilium longiflorum Thunb.leaf cultures were studied.The aim of the research is to study the regeneration ofi. longiflorum via somatic embryogenesis of leaf explant.IAAat5 level of concentrations (0-1 mg/1) combined with BAP at 4 level of concentrations (0-2 mg/1) were tested to induction of somatic embryogenesis in the first stage. Combination of two cytokinins, namely BAP and kinetin, combined with either of four auxins, namely IAA, IBA, NAA or 2,4D were tested to induce the somatic embryo genesis in the second stage. Combination of two concentrations of ABA, namely 0 and 1 mg/1 combined with 5 level concentrations of GA3, namely 0, 0.25, 0.5, 0.75 and 1 mg/1 were tested to improve the somatic embryos maturation.The results showed that somatic embryos were promoted by addition of IAA and BAP. The study of auxin and cytokinin combinations on somatic embryogenesis showed that somatic embryogenesis of kerk lily induced by addition of auxin and cytokinin in the culture medium.The maximum somatic embryo induction was obtained on medium contained of 0.25 mg/1 IAA combined with 1 mg/1 kinetin. Somatic embryos maturation were improved by transferring the somatic embryo from induction medium to maturation medium that contained 0.5 mg/I GA3 +1 mg/1 ABA.
PENGARUH ARAH DAN UKURAN POTONGAN SISIK UMBI KERK LILY (Lilium longiflorum Thunb.) TERHADAP PEMBENTUKAN TUNAS MIKRO DAN BULBLET SECARA IN VITRO Priyono Priyono; Sri Winarsih
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1102

Abstract

Mass production by using micro propagation technique has an important role for Lilium longiflorum regarding that it can produce seedlings in relatively short time, disease free, and regularly time. Several factors affected the successful micro shoots and bulblets formation, i.e.medium, explant, and temperature. This research aimed to know the effect of direction and width of scale section on the multiplication of kerk lily in vitro.Research has been conducted in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute, Jember.The research was arranged in factorial completely randomised design, i.e.direction of scale section and width of scale section. Two levels of direction of scale section namely longitudinal and transversal and five levels of width of scale section namely 1, 4, 6, 8, and 10 mm.The results showed that scales are able to produce micro shoot only, bulblet only, or both micro shoot and bulblet.The number of micro shoots produced from longitudinal section are higher than those of produced from transversal section. Width of scale section affected micro shoots and bulblets production, which their correlations are quadratic.The trend of micro shoots production was contrary with bulblet production.
Co-Authors Achmad Tavip Junaedi Adi Rahayu Adilia Galuh Pramantari AF Kurniawan Agung Putra Leksana Agus Anggoro Sigit Agustin Sri MULYATNI Ahmad Dardiri Alfiyyah Nurlaili Sukma Alif Noor Anna andi gunawan Antelas Eka Winahyo Atien Priyanti Aulia Aulia Bram Brahmantiyo Budiyanto Budiyanto Choirul Amin Chotimah Chotimah Damianus Manesi Danardono Danardono Danis Novianto Deden Dewantara ERIS Dewi Liesnoor Setyowati Dilahur Dilahur Dina Syafrianingsih Dwi ASTUTI Dwi Priyanto Edy Suprapto Eka Fitriana Elin Karlina Endang Sri Sudalmi Endang Sri Suldalmi evelyn wijaya Evy Khuriyana Fadila Fitria Wulandari Fadrul Fadrul Fahrizal Fahrizal Gunawarman Gunawarman H. Sutanto Hadi Wasito HAPPY WIDIASTUTI Henny Soepriyanti I Nurhasanah Ihwan Susilo Intan Apriadi Irma KRESNAWATY Isnandar Isti’ana Maftuchah Jumadi Jumadi K. S. Firdausi Kartika Cindi Wulandari Karyono Karyono Krisma Dwi Rohana Kuswaji Dwi Priyono Kuwat Triyana Laksono Trisnantoro Lina Ayu Widyastuti Linuwih Adhi Anggoro M. Rizal At-Thariq Made Wena Maria Erviana Klau Meliana Ida Pingki Rukmana Mochamad Soelton Mochamad Sofwan Muhammad Faisal Muhammad Farid Aminudin Nini Daniah Novita Eka Kumalasari Nur Fadjrih Asyik Nuzuliarini Nuris Pepri Pratama Pribadi Pribadi R. Mahmud Sugandi Rachmat Suryadithia Rian Rosartio Riyanti Sinaga Rizki Ardianto Nugroho Rizky Handayani Rudiyanto Rudiyanto Ruska Wati Saiful Bahri Sapto Riyadi Sigit Widiyarto Siswadi Siswadi Siti Maryam Sri Indriyanti Stefani Chandra Sudirman Wilian Sugiyanto Sugiyanto Sukarya Danimihardja Teddy Chandra Tegar Dadang Dadang Tri Kuncoro Tri Panji Triyono, Kharis U Kaltsum Udin Udin Untung Waluyo Wita KIMBERLY Wofrid E. Bianome Yohana Natalia Attik Primastuti Yuli Priyana Yusmarnida Yusmarnida Zempi, Chairun Nisa