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EFFECT OF FERMENTATION TIME ON MINERAL PROFILE AND TOTAL MOLD OF COWPEA (Vigna unguiculata) TEMPEH Rahmawati, Irma Sarita; Dyanti, Ghina Putri; Madani, Muhammad Surya; Widyanto, Rahma Micho; Istifani, Lola Ayu; Maulidiana, Annisa Rizky; Aisyiyah, Ekkel Lintang
Jurnal Pangan dan Agroindustri Vol. 11 No. 4: October 2023
Publisher : Department of Food Science and Biotechnology, Faculty of Agriculture Technology, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpa.2023.011.04.2

Abstract

Tempe is one of the popular foods in Indonesia which comes from fermented soybeans with the help of Rhizopus oligosporus. The development of tempeh with non-soy ingredients is starting to be carried out frequently. Cowpea (Vigna unguiculata subsp. unguiculata) is one of the nuts that is often consumed by Indonesian people. Cowpeas can be an alternative substrate for tempeh fermentation, because of their nutritional content, including vitamins and minerals, which are quite good for the body. However, some of these minerals are still bound to the phytic acid compound so it needs to be explained by the fermentation process. This research aimed to determine the ash content, mineral content of iron, calcium, and total mold in cowpea tempeh at a fermentation time of 35, 45, and 54 hours using standard methods. The research design used was a Completely Randomized Design (CRD) with repetition 3 times for each treatment for fermentation duration P1 (35 hours), P2 (45 hours), and P3 (54 hours). The ash and calcium content of cowpea tempeh decreased with fermentation times of 45 and 54 hours compared to 35 hours. In terms of iron content, the amount was almost the same at all fermentation times. Meanwhile, the total number of molds increased with fermentation times of 45 and 54 hours. Based on the results of observations for further research, the fermentation time for making cowpea tempeh used in this optimization was determined to be 35 hours. A fermentation time of 35 hours produces good cowpea tempeh, high calcium content, and efficient time and energy.
Mothers’ experiences implementing Local Food-Based Feeding Recommendations (LFFRs) for stunted children in Malang, Indonesia: a narrative study Nastaina, Hayu Iyaka; Wirawan, Nia Novita; Maulidiana, Annisa Rizky
AcTion: Aceh Nutrition Journal Vol 10, No 4 (2025): December
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v10i4.2688

Abstract

Local Food-Based Feeding Recommendations (LFFRs) were developed to fulfill the nutritional needs of stunted toddlers but remain underexplored in community settings. This study systematically examined child feeding practices based on LFFRs and investigated the contextual factors, challenges, and strategic efforts influencing their implementation by mothers of toddlers. A qualitative narrative design was used. Six mothers of toddlers were purposively selected and participated in in-depth interviews, each lasting 45–60 min. Data collection was conducted over two months. Thematic analysis was used to identify key patterns, and source triangulation was applied to enhance the credibility and trustworthiness of the findings. The analysis of the interview data revealed five main categories and 12 subthemes. These main categories were: (1) Overview of LFFRs implementation, (2) Factors influencing TIPs LFFRs, (3) Challenges in implementing TIPs LFFRs, and (4) Strategies to improve children's intake.  The findings revealed varied consumption patterns, with animal-based proteins (mainly eggs and chicken) dominating, limited intake of plant-based proteins (primarily tofu and tempeh), and fluctuating fruit, vegetable, and dairy consumption. Key barriers included children’s food preferences, time constraints, limited knowledge of local food preparation (e.g., wader fish and fresh milk), and perceived food-related risks. Adaptive strategies, such as diverse food processing and small frequent feedings, were employed by some mothers. These findings highlight the need for context-specific nutrition education and practical support for mothers, complemented by further research, evidence-based visual modules, practical caregiver training, and strengthened cross-sectoral collaboration to ensure the sustainable implementation of LFFRs and local food availability.
Effect of Probiotics on Respiratory Infections in Stunted Preschool Children: A Quasi-Experimental Pre-Post Study Deslina Rohmatul Luklu; Fajar Ari Nugroho; Annisa Rizky Maulidiana
Indonesian Journal of Human Nutrition Vol. 12 No. 2 (2025)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2025.012.02.8

Abstract

Stunting in preschool-aged children increases susceptibility to upper respiratory tract infections (URTIs) due to impaired immune systems and imbalances in gut microbiota. This study aimed to evaluate the effect of probiotic supplementation on URTI incidence among stunted preschool-aged children and to support nutrition-based preventive strategies for reducing acute respiratory infections among vulnerable populations. This study used a quasi-experimental one-group pre-test-post-test design conducted over 120 days from August 2023 to January 2024. Fifteen stunted preschool-aged children enrolled in a kindergarten in Probolinggo City participated through total sampling. Data were collected using health status forms completed by enumerators during school days and by parents during holidays. Observations were conducted during two consecutive 60-day periods before and during probiotic supplementation. The intervention consisted of daily administration of 65 ml of a probiotic containing more than 100 million Lactobacillus casei Shirota strain bacteria per milliliter. Statistical analysis included Fisher’s Exact Test and the Wilcoxon Test with a significance level of p < 0.05. Results showed a significant difference in URTI status before and during probiotic supplementation (p = 0.042) and in the duration of school absenteeism (p = 0.011). However, no significant differences were found in URTI episode frequency, episode duration, or overall illness duration. The study concludes that probiotic supplementation (65 ml) has a significant impact on reducing URTI occurrence and school absenteeism in stunted preschool children.