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All Journal Jurnal Ilmu Lingkungan Jurnal Bioterdidik: Wahana Ekspresi Ilmiah BERITA BIOLOGI BIOMA : Jurnal Biologi Makassar Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Ilmiah Hijau Cendekia Jurnal Ilmiah Farmasi Farmasyifa Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Gunung Djati Conference Series JURNAL BIOLOGI PAPUA AMMA : Jurnal Pengabdian Masyarakat

Bambang Irawan

FMIPA Universitas Lampung

Author-ID : 3527275

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MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.) Nadya Febri Harlifia; Bambang Irawan; Salman Farisi; Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.163

Bamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.)

BROMELAIN WASTE TEA COMPOST INDUCED BY LIGNINOLYTIC INOCULUM OF Trichoderma sp. ON THE GROWTH OF LEAF NUMBER AND CHLOROPHYLL CONTENT OF CHILI (Capsicum annuum L.) Syafira Clarisa Huda; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.165

Pineapple is one kind of fruit that contains a lot of nutrition. The waste from residual pineapple processing is called bromelain. Bromelain contains polymer complex in the form of organic sources from nature if it could be decomposed properly. One way to exploited bromelain is by composting. Composting is done with the help of fungi of Trichoderma sp. to speed up the decomposition process. The resulting compost is aerated to increase the nutrients and form a further decomposition process in the soil. The research was carried out from January till March 2021 in the Microbiology Laboratory Biology, Faculty of Mathematics and Science, University of Lampung. The parameters of this research are the content of chlorophyll and the number of leaves. This research used a completely Randomized Design with 7 treatments that are A1 (24 hours of mixed bromelain), A2 (48 hours of mixed bromelain), A3 (72 hours of mixed bromelain), A4 (24 hours of pure bromelain), A5 (48 hours of pure bromelain), A6 (72 hours of pure bromelain), and A7 (control). Based on this research the value of the highest chlorophyll A on A6 treatment is 0,655. A value of the highest on chlorophyll B on A6 treatment that 0,429. The highest total chlorophyll on the A6 treatment is 1,048. Based on this research the A4 treatment has the highest average number of leaves compared to other treatments.

APPLICATION OF BROMELAIN LITTER SOLID COMPOST INDUCED BY LIGNINOLITIK Trichoderma sp. FUNGUS TOWARDS NUMBER OF LEAVES AND CHLOROPHYL CONTENT CHILI PLANTS (Capsicum annuum L.) Jihan Haura; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.166

Piles of uncontrolled pineapple peel waste have a negative impact that will affect various aspects of life, either directly or indirectly. Therefore, pineapple peel waste that can no longer be processed can be used for making compost. The taste of pineapple can be degraded biologically with the help of enzymes that can be produced by microorganisms. One of the microorganisms that can support the decomposition process is a group of fungi. In this study, the fungus Trichoderma sp. (Bioggp 5) is a ligninolytic fungus. Trichoderma sp. is a saprophytic soil microorganism that attacks natural pathogenic fungi and is beneficial to plants. The purpose of this study was to determine the effect of solid bromelain compost inoculum of ligninolytic fungi (Trichoderma sp.) on leaf number and chlorophyll content of chili plants (Capsicum annum L.) and the best dose of compost that could be used. This study used a completely randomized design (CRD) with 7 treatments, namely T0 (control), T1 (1.4% pure bromelain), T2 (1.7% pure bromelain), T3 (2% pure bromelain), T4 (1, 4% bromelain + leaf litter), T5 (1.7% bromelain + leaf litter), T6 (2% bromelain + leaf litter). The parameters used were the number of leaves and chlorophyll content. And the results obtained from this study were the T3 dose (2% pure bromelain) was the best dose for the number of leaves, while the T2 dose (1.7% pure bromelain) was the best dose for chlorophyll content.

Pertumbuhan Vegetatif Eksplan Kentang (Solanum Tuberosum L.) Kultivar Atlantik pada Medium Murashige and Skoog dengan Penambahan Ekstrak Tomat (Solanum Lycopersicum L.) Secara In Vitro Moza Fierda Atiek; Endang Nurcahyani; Bambang Irawan
Jurnal Bioterdidik: Wahana Ekspresi Ilmiah Vol 10, No 3 (2022): JURNAL BIOTERDIDIK
Publisher : Universitas Lampung

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This research was to find out the growth of potato explant (Solanum tuberosum L.) atlantic cultivar after giving concentrations of tomato (Solanum lycopersicum L.) extract and to find out the optimum concentrations of tomato extract for the growth of potato (Solanum tuberosum L.) explant Atlantic cultivar in vitro. This research uses a completely randomized design with a single factor, with 5 concentrations levels : 0%,2%,4%,6%,8%. Each treatment was repeated 5 times. Data obtained then in Levene test, then carried analysis of varience test at the 5% real level. The result of this research indicate that giving tomato extract to vegetative growth of potato had not significant of height, the amount of leaves, and the amount of shoots. Keywords: growth, in vitro, Solanum tuberosum L., Tomato extract Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak tomat (Solanum lycopersicum L.) terhadap pertumbuhan vegetatif eksplan kentang (Solanum tuberosum L.) kultivar Atlantik secara in vitro. Penelitian ini dilaksanakan pada bulan November 2018 sampai bulan Desember 2018 di Laboratorium Botani (ruang penelitian in vitro), Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung menggunakan pola dasar Rancangan Acak Lengkap (RAL) yang terdiri dari 1 faktor yaitu ekstrak tomat dengan lima taraf konsentrasi, yaitu 0% v/v, 2 % v/v, 4% v/v , 6% v/v, 8% v/v. Penelitian ini dilakukan dengan 5 ulangan sehingga total botol yang digunakan berjumlah 25 botol. Parameter yang diamati pada penelitian ini yaitu tinggi planlet, jumlah daun dan jumlah tunas. Data yang diperoleh di homogenkan menggunakan uji Levene, kemudian dilanjutkan uji ANARA pada taraf nyata 5%, jika signifikan maka dilakukan uji Lanjut pada BNT taraf 5%. Hasil penelitian menunjukkan bahwa penambahan ekstrak tomat tidak berpengaruh nyata terhadap tinggi tanaman, jumlah daun dan jumlah tunas. DOI: http://dx.doi.org/10.23960/jbt.v10.i3.24967

POTENSI ANTAGONIS Bacillus sp ASAL KEBUN RAYA LIWA (KRL) SEBAGAI AGEN PENGENDALI JAMUR Fusarium sp Indriani Indriani; Christina Nugroho Ekowati; Kusuma Handayani; Bambang Irawan
Gunung Djati Conference Series Vol. 18 (2023): Prosiding Seminar Nasional Biologi (SEMABIO) Tahun 2022
Publisher : UIN Sunan Gunung Djati Bandung

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Fusarium sp is a type of fungus that is pathogenic especially on plants. This fungus attacks the pith tissue of the stem through the injured root. Yellow leaf color is an early indication of this fungal infection. An alternative in controlling this fungus could be to use Bacillus sp because these bacteria have growth inhibitory compounds. This study aims to determine the effect of Bacillus sp from Liwa Botanical Garden on Fusarium sp. Antagonist testing using dual culture method on media Mueller Hinton Agar (MHA) with 3 bacterial isolates was tested. The results of the antagonist test showed that the isolate Bacillus sp.TSR 6 had an inhibitory capacity of 19.7 mm with a strong inhibitory category against the fungus Fusarium sp.

Pemberian Kompos Bahan Sampah Serat Bromelain Yang Diinduksi Inokulum Fungi Selulolitik Aspergillus sp. Pada Pertumbuhan Vegetatif Cabai (Capsicum annuum L.) Fadila Raisyadikara; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Biologi Papua Vol 14, No 1 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The purpose of this research is to understand the effect and the best dossage of bromelain wastes solid compost for vegetative growth of chilli plants. Accumulation of pineapple wastes made by the large pineapple plantation area. The litter of pineapple bromelain enzyme (bromelain wastes) still containing organic matter that could composted for plant nutrition. The composting of it can be accelerated by induction inoculum of cellulotic Aspergillus sp. fungus. The research used Completely Randomized Design method with seven treatment and three replications. The treatment used two different type of compost. The composition of compost A type is: 0,5 kg bromelain + 0,5 kg leaves litter + 0,5 kg livestock wastes + inoculum 1%. The composition of compost B type is: 1 kg bromelain + 0,5 kg livestock wastes + inoculum 1%. The treatment conducted P0: 5 kg soil (control). P1: 5 kg soil + 1,4% A type compost. P2: 5 kg soil + 1,7% A type compost. P3: 5 kg soil + 2% A type compost. P4: 5 kg soil + 1,4% B type compost. P5: 5 kg soil + 1,7 % B type compost. P6: 5 kg soil + 2% B type compost. Variance analyzed of obtained data were using ANOVA and tested using the Least Significant Difference Test (LSD) method. Parameter of this study are number of leaves and chlorophyll content. The results showed that the application of P6: 2% dossage B type compost from bromelain solid compost could optimized the vegetative growth of chili plants (Capsicum annuum L.). Key words: Aspergillus sp.; bromelain; chili; compost.

Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L. Wuri Artikasari; Emantis Rosa; Bambang Irawan
Jurnal Biologi Papua Vol 11, No 2 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.

Artikel Review: Aplikasi Inokulum Fungi Trichoderma spp. Untuk Pertumbuhan dan Penekan Fitopatogen Ega F.S. Bina; Bambang Irawan; Wawan A. Setiawan; Christina N. Ekowati
Jurnal Biologi Papua Vol 14, No 2 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The growth of a plant is influenced by the availability of nutrients. To meet these nutritional needs, chemical fertilizers are still widely used. In addition to nutritional needs, the growth of a plant is also influenced by pathogenic microorganisms that cause disease. The emergence of diseases in plants causes chemical pesticides to be increasingly used. The continuous use of chemical fertilizers and chemical pesticides can make living organisms susceptible to high toxicity of chemical compounds. In addition, the impact has an impact on improper management of agricultural waste as well as polluting the environment when it has been burned or discharged into water bodies. One alternative to overcome these problems is the application of biological control using Trichoderma spp. Trichoderma spp inoculum application can significantly regulate the rate of plant growth and suppress the growth of plant pathogenic microorganisms. Trichoderma spp. including plant growth promoting microbes that have the ability to colonize plant roots so as to provide benefits to their host, modulate phytohormonal production, increase soil nutrient availability, stimulate plant growth and tolerance to biotic and abiotic stresses, and resistance to pathogens. Inoculum of Trichoderma spp. can be applied to plants through seeds, leaves, roots of seedlings, and soil. Based on literature review, it is known that the application of Trichoderma spp. inoculum showed a significant effect on plant growth and suppress the growth of pathogenic microorganisms.Key words: Trichoderma spp.; inoculum; fungi; biofertilizer; biofungicide.

Produksi Cabai Merah (Capsicum annuum L.) dari Benih yang Diinduksi Medan Magnet 0,2 mT dan Diinfeksi Jamur Fusarium sp. Rochmah Agustrina; Herta M. Manullang; Bambang Irawan; Sri Wahyuningsih; Sumardi Sumardi
Jurnal Biologi Papua Vol 12, No 1 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

As one of the horticultural plants that has important economic value, the cultivation of red chili (Capsicum annuum L.) is often constrained by the attack of Fusarium sp., which can cause losses due to decreased production. Induction of the magnetic field on the plant is known to be able to increase growth, development, and production in various plants. In this research, we studied the production of chili grown from seeds exposed to a 0.2 mT magnetic field and then infected with Fusarium sp. The study was conducted factorial using a completely randomized design with 2 factors. The first factor is the exposure of a 0.2 mT magnetic field to the seeds consisting of controls, without exposure to a magnetic field (M0), magnetic field exposure for 7 minutes 48 seconds (M7), and 15 minutes 30 seconds (M15). The second factor is Fusarium sp. infection on seeds that have been exposed to a magnetic field for 60 minutes by immersion of the seeds with spore suspension of Fusarium sp. with the density of 1 x 107 conidia/ml (F60), and without Fusarium sp. infection (F0). Each treatment unit was repeated 5 times. The data obtained were analyzed for variance at α = 5% followed by the least significant difference test using the Fishers Test at α = 5%. The results of data analysis showed that the interaction of 0,2 mT magnetic field exposure and infection of Fusarium sp. in chili seeds significantly affect the stomata index, the fruiting rate, and the number of fruit but do not affect the content of total chlorophyll, carbohydrate, as well as the number of flowers. The highest fruiting rate and number of fruits obtained from the treatment result of 0.2 mT magnetic field exposure for 15 minutes 36 seconds without Fusarium sp. infection (M15F0). Infection of Fusarium sp. in these seeds (M15F60) reduce the fruiting rate and the number of fruit but it is not significant and even almost similar with the fruiting rate and the fruit number of the plant growth from control treatment (M0F60). Key words: magnetic field, Fusariumsp., seed, and production.

Pelatihan Kewirausahaan Pembuatan Kompos Unggul Dari Seresah Daun Dengan Induksi Inokulum Fungi Pada Mahasiswa Program Studi Bahasa Arab Sekolah Tinggi Ilmu Tarbiyah Darul Fattah Bandar Lampung Salman Farisi; Bambang Irawan; Suratman; Sutyarso; Hendri Busman
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 8 : September (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

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Compost is the final substance in the fermentation process of piles of waste/plant litter including animal carcasses. The fermentation needs to be accelerated with human assistance. This saprotrophic fungi isolate has the potential to be developed as a compost inoculum in the manufacture of organic fertilizers. Compost induced with fungal inoculum is able to produce organic fertilizer relatively quickly and contains high plant nutrients. The prospect of the need for organic fertilizers is quite high in supporting organic agriculture, so it is necessary to disseminate information on science and technology and training to the public on the manufacture of organic fertilizers by induction of saprotrophic fungi inoculum. In addition, the availability of garden plant litter is abundant and free from chemical residues. The results of this activity showed that there was an increase in the knowledge and understanding of the training participants in making compost from leaf litter. The average increase in the knowledge of the trainees is 25,25 %.

Co-Authors Agung Abadi Kiswandono Agustrina, Rochmah Billi Andreas Christina N. Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Ega F.S. Bina Emantis Rosa Endang Nurcahyani Fadila Raisyadikara Fesya Salma Putri Hendri Busman Herta M. Manullang Indah Selfiana Indriani Indriani Intan Poespita Windiyani Jihan Haura Kinasih Cahyono Kusuma Handayani Martha Lulus Lande Martha Lulus Lande Masniar Vina Kesti Moza Fierda Atiek Nadya Febri Harlifia Priyambodo Priyambodo Rista Wahyu Mudya Salman Farisi Shofia Rodia Sri Wahyuningsih Sumardi, Sumardi Suratman Suratman Suratman Suratman Sutyarso Syafira Clarisa Huda T. Indah Setia Ningsih Tundjung T. Handayani Wanda Amelia Wawan Abdullah Setiawan Wuri Artikasari Yulianty Yulianty Yulianty Yulianty Zulkifli Zulkifli zulkifli zulkifli

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