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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Buletin Tanaman Tembakau, Serat & Minyak Industri Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Entomologi Indonesia Buletin Tanaman Tembakau, Serat & Minyak Industri Jurnal HPT (Hama Penyakit Tumbuhan)

Bambang Tri Rahardjo

Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya

Author-ID : 354501

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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The Effect of Inoculum Type and Mycorrhiza Dosage on Growth and Production of Mung Bean Vigna Radiata L. Syamsuddin Djauhari; Jami'at Dwi Elriyono; Bambang Tri Rahardjo
Journal of Tropical Plant Protection Vol 2, No 1 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.1.4

Mung bean (Vigna radiata L.) is the third most essential legume crop commodity after soybeans and peanuts. Mung bean production faced problems of nutrient-poor land and soil texture that does not support soybean growth. The solution to overcome these obstacles is the application of mycorrhizae in nutrient-poor soils. This study aimed to determine the effect of various inoculums and mycorrhizal doses on growth and production in mung beans. This study used a Completely Randomized Design with nine treatments and three replications. The research included mycorrhizal inoculation with various treatments, as well as observations with variable plant height, plant biomass, number of pods, wet weight and dry weight of pods, the weight of 50 wet and dry seeds, mycorrhizal infection in roots, and number of spores in 100 gr of soil. The data were analyzed by SPSS 15.0 program for ANOVA. The results indicated that granular (30 gr) had the highest plant height of (41.33 cm), crown weight (30.69 gr), number of pods, wet and dry pod weight is 13.66, 9.84 gr, and 8.86 gr, respectively, 50 kg of wet and dry weight (3.82 and 3.20) and the highest effect on the number of spores at 29.33. The 30 gr tablets treatment showed the highest results on root weight (22.98 gr), while the 30 gr mixture showed the highest results on the percentage of infection (73.33%).

The Effect of Insecticide Application on Plutella xylostella Linn. and its Parasitoid Diadegma sp. Bambang Tri Rahardjo; Tita Widjayanti; Antika Anggraini
Journal of Tropical Plant Protection Vol 2, No 1 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.1.1

Cabbage is one of the commodities that mostly cultivated in Indonesia, but the cabbage farmers often suffered losses because of pests' attack. The important pests on cabbage is Plutella xylostella. This study aimed to investigate the effectiveness level of an insecticide with active ingredient carbaryl 85% in controlling P. xylostella which attacks cabbage and its impact on the parasitoid of Diadegma sp. This research uses a randomized block design with six treatments and four replications. The treatments were different concentrations of the insecticide carbaryl 85%. The testing on Diadegma sp. used two methods, directly and indirectly test (residual exposure). The carbaryl 85% was not effective in controlling P. xylostella, but the population was decreasing. The carbaryl 85% with the highest effect in reducing P. xylostella population was 3.0 g/l. The carbaryl 85% did not affect on the mortality of natural enemies (Diadegma sp. parasitoid) after direct and indirect test.

KEANEKARAGAMAN DAN KEKERABATAN LALAT BUAH (DIPTERA: TEPHRITIDAE) DI KALIMANTAN SELATAN BERDASARKAN KARAKTER MORFOLOGI DAN MOLEKULAR (RAPD-PCR DAN SEKUENSING DNA) M Indar Pramudi; Retno Dyah Puspitarini; Bambang Tri Rahardjo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Diversity and phylogeny of fruit fly (Diptera: Tephritidae) in South Kalimantan based on morphology and molecular (RAPD-PCR and DNA sequencing). Seven species of fruit fly was known by morphological identification. The fruit flies were found fromÂ trapping with methyl eugenol and fruit collecting at all study sites in South Kalimantan. The results showed that as much as 17Â plants were infected by fruit fly. Dendrogram based on morphological identification analyzed by using UPGMA with MEGA 4 program consisted in a group consisting of 5 sub-groups. Bactrocera carambolae and Bactrocera papayae of morphology were still a closely related fruit fly at 0.935. Whereas, based on RAPD result analized by UPGMA using 20 character of DNA based, showed that out of seven species consisted 2 groups, 1st group were B. umbrosa,Â B. occipitalis and sub-group of B. latifrons. The second group consists of sub-groups B.carambolae, B. papaya, sub-group B. albistrigata and B. cucurbitae. The results of dendrogram from sequencing DNA fruit fly analysis comprised one of group and three sub-groups. The first sub-groups were B. papayae, B. carambolae, B. occipitalis, B.latifrons. The second subgroup were B. cucurbitae and B. umbrosa. While B. albistrigata separate but still one group with another fruit flies. The results of DNA sequencing showed that there were a homology of the seven species of the fruit fly i.e at 83 base pair / bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A ). The length of the base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons were respectively 615, 898, 570.969, 898 and 615 bp. The results of morphological analysis and RAPD methods showed difference in the distribution of groups and sub-groups. But based on morphologycal and DNA identification seven species of fruit flies found were all same as the genebank.

Sugarcane Leaf Litter as Soil Amendment to Stimulate Collembolan Diversity Bambang Tri Rahardjo; Rina Rachmawati; David Soetjipto
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.2245

The sugarcane leaf litter contains organic material and if it is returned to the soil, it will increase the diversity of Collembola. Collembola has an important role as a decomposer of soil organic matter and bioindicator of soil health. This study was conducted to determine the effect of sugarcane leaf litter on the diversity of Collembolan in sugarcane plantation at Kebonagung Sugar Factory, Malang. This study was conducted from March to June 2018. Transect method was used in sampling method. The pitfall traps were used to obtain Collembolan samples on the surface of the ground while the Berlese-Tullgren funnels were used to obtain Collembolan samples inside the soil. A total of 5,535 collembolan were collected. Five species were collected, including Brachystomella sp., Folsomides sp., Mesaphorura sp., Alloscopus sp., and Dicranocentrus sp. Applying sugarcane leaf litter to the soil has a positive effect on Collembolan diversity, as evidenced by the increasing value of diversity index on the soil surface from 0.53 to 1.11 and decreasing the value of dominance index on the soil surface from 0.76 to 0.43.

Pengaruh PGPR terhadap Penekanan Populasi Nematoda Puru Akar (Meloidogyne incognita (Kofoid and White) Chitwood) pada Tanaman Kenaf (Hibiscus cannabinus L.) Kristiana Sri Wijayanti; Bambang Tri Rahardjo; Toto Himawan
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 8, No 1 (2016): April 2016
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Tanaman kenaf yang terinfeksi nematoda Meloidogyne incognita dapat mempengaruhi pertumbuhan tanaman dan produksi serat. Penelitian ini bertujuan untuk mengevaluasi pengaruh Plant Growth Promoting Rhizobacteria (PGPR) dalam menekan populasi nematoda M. incognita pada tanaman kenaf di rumah kaca. Penelitian dirancang dengan menggunakan rancangan acak kelompok (RAK) faktorial dengan dua faktor, faktor pertama adalah cara aplikasi PGPR yang terdiri atas 2 cara yaitu suspensi PGPR diberikan sebelum tanam dengan merendam benih selama 5 jam (C1), benih ditanam langsung dalam pot tanpa direndam dalam PGPR (C2), dan suspensi PGPR diberikan pada 15 hari setelah tanam (HST) dan 25 HST. Faktor kedua adalah jenis PGPR yang digunakan yaitu Pseudomonas fluorescens, Bacillus subtilis, Azotobacter sp., P. fluorescens + B. subtilis, P. fluorescens + Azotobacter sp., B. subtilis + Azotobacter sp., dan P. fluorescens + B. subtilis + Azotobacter sp., serta kontrol (tanpa PGPR). Hasil penelitian menunjukkan bahwa perendaman benih dengan kombinasi tiga bakteri memberikan pengaruh yang nyata terhadap populasi juvenil nematoda dalam tanah, sedangkan perlakuan tanpa perendaman tidak memberikan pengaruh. Populasi juvenil nematoda di dalam akar yang diberi PGPR baik tunggal maupun kombinasi melalui perendaman benih atau tanpa perendaman benih tidak berpengaruh, kecuali pada kombinasi P. fluorescens dan B. subtilis yang diberikan melalui perendaman benih mampu menekan populasi juvenil nematoda di akar 43,28% bila dibandingkan tanpa perendaman benih. Pemberian rizobakteri P. fluorescens menurunkan jumlah telur nematoda terbanyak (86,39%) dan menekan intensitas penyakit sebesar 71,95% bila dibandingkan kontrol.Infection of Meloidogyne incognita on kenaf could affect its growth and the production of fiber. This study aimed to evaluate the effect of PGPR on the reduction of nematode M. incognita population on kenaf in the greenhouse. The factorial experiment was laid on randomized block design. The study consisted of two factors with three replicates . The first factor was method of PGPR application, ie: PGPR suspension was given before planting (kenaf seeds was soaked for 5 hours) (C1) and the seeds directly planted without submerged (C2), PGPR suspension was given at 15 days after planting (DAP) and 25 dap. The second factor was type of bacteria (Pseudomonas fluorescens, Bacillus subtilis, Azotobacter sp., P. fluorescens + B. subtilis, P. fluorescens + Azotobacter sp., B. subtilis + Azotobacter sp., and P. fluorescens + B. subtilis + Azotobacter sp.) and control. The results showed that submerged seed with the three bacterial rhizobacteria significant compared to the control treatment and single treatment and two combination rhizobacteria, while without submerged seed with single or combination rhizobacteria not significant on the population of juvenile nematodes in the soil. Combination of P. fluorescens and B. subtiliswith submerged seed capable of suppressing the population of juvenile nematodes in the roots of 43.28% when compared with or without submerged seed. Population of juvenile nematodes in the roots by submerged seed and without submerged seed either single or combination rhizobacteria do not affect each other. P. fluorescens suppress nematode eggs are highest 86.39% and disease intensity by 71,95% where compared to control.

Populasi Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) pada beberapa umur tanaman kelapa sawit Bambang Tri Rahardjo; Akhmad Rizali; Ika Putri Utami; Sri Karindah; Retno Dyah Puspitarini; Bandung Sahari
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Elaeidobius kamerunicus Faust is an oil palm pollinator insect that lives on the male inflorescence and visits female inflorescence to pollinate due to attracted by its volatile compound. The number of fruit sets of oil palm relates to the population of E. kamerunicus on a plantation. The objective of this study was to investigate the effect of age of oil palm on the population of E. kamerunicus. Field research was conducted in oil palm plantations that located in Pangkalan Lada, Kotawaringin Barat District, in Central Kalimantan. The research method was by measurement of E. camerunicus population on male inflorescences and set up the sticky trap on female inflorescences. Plot with size 7000 m2 (100 trees) was selected on several ages of oil palm i.e. 6, 10 and 16 years. Each plot, the number of male and female inflorescences was counted and some inflorescences were chosen for measurement of E. camerunicus population that was conducted monthly for three months of observation. The results showed that the age of the oil palm affected the population of E. kamerunicus in male inflorescences but not in female inflorescences. The older age of oil palm, the population of E. kamerunicus in male flowers was increasing. The sex ratio of E. kamerunicus found in male and female inflorescences of oil palms tend to female bias. Based on the comparison of E. kamerunicus population between male and female inflorescences on each plot, it was found that visitation value of E. kamerunicus was highest in the young oil palm. In conclusion, increasing age of oil palm trees affected on increasing of E. kamerunicus population in male inflorescences, but visitation value on female inflorescences prone to decline.

POTENSI ASAP PEMBAKARAN TEMPURUNG KELAPA DALAM PENGENDALIAN HAMA Rhyzopertha dominica F. (COLEOPTERA: BOSTRICHIDAE) PADA GABAH DALAM SIMPANAN A. A. Ketut Aryawan; Bambang Tri Rahardjo; Ludji Pantja Astuti
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 1 (2013)
Publisher : Universitas Brawijaya

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ABSTRACTUtilization of smoke as a fumigant, has long been done to control pests whichattack the material in storage. Rhyzopertha dominica is avprimary pest of rice instorage. Heavy attack of R. dominica on the rice storage could caused yield lossesabout 7% during 6 months. Larvae and imago R. dominica attacking growingpoint and the endosperm of seeds, causing serious losses. A research to studycharacteristics and effect of smoke burning coconut shell to R. dominica and theinfluence of rice germination/viability. The research method used CompletelyRandomized Design with fumigation time treatment. Fumigation treatmentconsisted of four level (0 hour as a control, 1 hour, 2 hours and 3 hours). Eachtreatment consisted of 4 replications. The results showed that smoke from burningcoconut shell can cause mortality to imago of R. dominica, causing lowpopulation of R. dominica, causing eggs damage, mortality to larvae of R.dominica, fumigation of rice during 1 hour, 2 hours and 3 hours, give no effect onrice germination, but on rice seedling height and fumigation time of paddy iseffective and efficient for controlling R. dominica is 2 hours.Keywords: rice, smoke, coconut shell, Rhyzopertha dominica

EKSPLORASI NEMATODA ENTOMOPATOGEN PADA LAHAN TANAMAN JAGUNG, KEDELAI DAN KUBIS DI MALANG SERTA VIRULENSINYA TERHADAP Spodoptera Litura Fabricius Liza Afifah; Bambang Tri Rahardjo; Hagus Tarno
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 2 (2013)
Publisher : Universitas Brawijaya

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ABSTRACTEntomopathogenic nematodes (EPNs) as biological agents, is consistedSteinernema and Heterorhabditis genus. Steinernema and Heterorhabditis whichbelonged to Steinernematidae and Heterorhabditidae families had closedrelationship to Xenorabdus and Photorabdus as bacterial symbionts respectively(Smart, 1995). Types of soil moisture, temperature, and a host affect thedistribution, survivability, host finding and reproduction of EPNs. To get theEPNs local isolates, exploration of isolates from local areas was needed. Thisresearch was aimed to identify genus and density of EPNs on the fields of corn,soybean and cabbage in Malang Areas and their virulence to the larvae ofSpodoptera litura. Research was conducted at laboratory of pest and disease(Nematological Unit), Plant Protection Department, Faculty of Agriculture,University of Brawijaya, from April to September 2012. EPNs were obtainedfrom corn field in Ngijo village, soybean field in Kendalpayak village, andcabbage field in Bumiaji village. EPNs were isolated from soil samples by usingTenebrio molitor larva. White trap method was used to attract EPNs from Thedead larva of T. molitor and the population of EPNs was counted. EPNs wereidentified based on the symptoms of color changes on the cuticle andmorphological characters. In addition, virulence of EPNs was also tested on the S. litura larva. The result showed that density of EPNs was highest on cabbage field (23.264 EPNs/0.25 ml). Density of EPNs on corn field was 16.976 EPNs/0.25 ml and the lowest density was on soybean field (15.664 EPNs/0.25 ml). Based on the color changes and morphological characters of EPN, on the cabbage field, EPNs were identified as Steinernema and Heterorhabditis and the others, EPNs were identified as Steinernema. Based on the virulence test of EPNs on S. litura larva, there was significant difference between EPNs isolate of corn and othersespecially for 24 hours after application.Keywords: identification, virulence, entomopathogenic nematodes (EPNs),Spodoptera litura

POPULASI DAN SERANGAN HAMA ULAT KANTUNG Metisa plana Walker (Lepidoptera; Psychidae) SERTA PARASITOIDNYA DI PERKEBUNAN KELAPA SAWIT KABUPATEN DONGGALA, SULAWESI TENGAH Ronny Pamuji; Bambang Tri Rahardjo; Hagus Tarno
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 2 (2013)
Publisher : Universitas Brawijaya

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ABSTRACTThis research was aimed to study the population, attack intensity of bagworm (M. plana) and it parasitoid on oil palm plantations. Sampling was conducted 4 times in blocks 1-9 Afdeling OK PT. Lestari Tani Teladan, Donggala, Central Sulawesi from August to October 2012. A systematic sampling method was used to observe M. plana population from each observation point. There were two stages of M. plana such as larvae and pupae. Both stages were checked their conditions to clarify their parasitoids. In addition, parasitoids were observed especially at fourth observation. Result showed that population of M. plana larva decreased during observation period (from August to October 2012). Opposite condition was occurred that population of pupa increased in same period of observation. Highest population of M. plana was recorder on third and fourth block of plantation. Number of parasitized pupae directly proportional to total of pupae (R2=0,95). Populations of parasitoid have not been able to control pests M. plana naturally. Some parasitoids were identified such as Brachymeria sp. (34%), Eurytoma sp. (13%), Entodoninae (17%), Tetrastichus sp. (24%), Tachinidae 9% and two species of Phygadeuontinae 2% and 1% for each.Key words: bagworm, Metisa plana, parasitoid, oil palm

Efikasi Nematoda Entomopatogen Heterorhabditis sp. Isolat Lokal terhadap Diamond Back Moth Plutella xylostella Bambang Tri Rahardjo; Hagus Tarno; Liza Afifah
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 2 No. 2 (2014)
Publisher : Universitas Brawijaya

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ABSTRAK Diamond back moth atau biasa yang dikenal dengan Plutella xylostella merupakan salah satu hama penting pada tanaman Brassicaceae. Hama ini bersifat kosmopolitan yang dapat ditemukan hampir di setiap daerah pertanaman kubis. Pengendalian hama ini umumnya menggunakan insektisida kimiawi. Salah satu alternatif pengendalian P. xylostella adalah dengan pemanfaatan nematoda entomopatogen Heterorhabditis sp. Penelitian ini bertujuan untuk mengetahui virulensi nematoda entomopatogen terhadap hama Plutella xylostella di Laboratorium. Penelitian dilaksanakan di Laboratorium Entomolgi, Fakultas Pertanian, Universitas Brawijaya pada bulan Agustus - November 2013. Hasil penelitian menunjukkan bahwa tingkat kepadatan populasi nematoda entomopatogen berpengaruh terhadap persentase mortalitas P. xylostella. Semakin tinggi kepadatan populasi nematoda maka semakin tinggi pula mortalitas larva P. xylostella. Nilai LT50 dengan konsentrasi nematoda entomopatogen 400 JI/ml tercapai setelah 37,96 jam setelah aplikasi. Â Kata kunci: Heterorhabditis sp., virulensi, Plutella xylostella

Co-Authors A. A. Ketut Aryawan A. A. Ketut Aryawan Abdul Latief Abadi Achadian, Etik Mar`ati Achmad Faisal Akbar Adistanaya, Radika Adrianto Marthinus Ndii Affandi, Rohmat Sayful Ahmad Muhlisin Ahmad Muhlisin Akhmad Rizali Annike Putri Damayanti Antika Anggraini Ayu Rosmayuningsih Ayu Rosmayuningsih Ayumi, Cicik Nur Bandung Sahari BANDUNG SAHARI Bobby Polii David Soetjipto Dede Durahman Dede Durahman Devi, Mia Prastika Fadloli, Ahmad Iqbal Rijal Fitriani, Ima Gatot Mudjiono Guntur Respyan Guntur Respyan Hagus Tarno Hagus Terno Hidayat, Mohamad Rizkiy Husni dan Zulfanazli Pudjianto Alfian Rusdy Ika Putri Utami Ika Putri Utami Isna Kartika Wati Isnainy Dinul Mursyalati Yus Isnainy Dinul Mursyalati Yus Jami'at Dwi Elriyono Jantje Pelealu Januarisya, Muthia Adira Karisma Aditya Wardani Kristiana Sri Wijayanti Kurnia Paramita Sari Laraswati, Nur Adinda Liza Afifah Liza Afifah Liza Afifah Ludji Pantja Astuti Ludji Pantja Astuti Ludji Pantja Astuti Manurung, Jihan Salmaa Afiifah Marianus Marianus Marwoto . Maspupah Huzni Maspupah Huzni Mawaddah, Rabbiana Meity Nelltje Tanor Mochammad Syamsul Hadi Mufidah Afiyanti Muhammad Indar Pramudi Muhammad, Faiz Nashiruddin Niko Mahendra Nur Angga Prayoga Nurlinda Nurlinda Nurul Aini Okty Ayu Lestari Praditya, Al Farriz Sandro Presti Mardiyani Purwaningtyas Presti Mardiyani Purwaningtyas, Presti Mardiyani PURNAMA HIDAYAT Putra, Satrya Adhimas Eka Putri Setya Rahmita Rahmawati, Alvina Eka Retno Dyah Puspitarini Rina Rachmawati Rina Rachmawati Rina Rachmawati Rizki Nur Suci Rizki Puji Widiastuti Rizki Puji Widiastuti Rizky Septiyawati Rohma Wulan Septiani Ronny Pamuji Ronny Pamuji Rose Novita Sari Handoko Senoaji, Wasis Silvi Ikawati Sri Karindah Sri Karindah Sri Karindah Sumeru Ashari Syamsuddin Djauhari Syamsuddin Djauhari Syamsulhadi, Mochammad Taufiqurrahman, Achmad Fitriadi Teguh Yulianto, Teguh Tita Widjayanti Tita Widjayanti Toto Himawan Toto Himawan Zeni Ningrum Zeni Ningrum

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