Article Per Year (5 Year)
p-Index From 2021 - 2026
1.607
P-Index
This Author published in this journals
All Journal Bioma : Berkala Ilmiah Biologi Jurnal Kesehatan Andalas Majalah Kedokteran Andalas PREPOTIF : Jurnal Kesehatan Masyarakat Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jurnal Ilmu Kesehatan Indonesia (JIKSI) Journal of General Procedural Dermatology and Venereology Indonesia Jurnal Riset Ilmiah Jurnal Sains, Teknologi dan Kesehatan
Roslaili Rasyid
Fakultas Kedokteran Universitas Andalas
Agriculture, Biological Sciences & Forestry Computer Science & IT Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 23 Documents
Search

 1   2   3 
UJI EFEKTIVITAS DISINFEKTAN YANG DIGUNAKAN UNTUK MEMBERSIHKAN LANTAI POLIKLINIK RSUP DR. M.  DJAMIL PADANG TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN  ESCHERIACHIA COLI Zulkarnain, Anugrah; Rasyid, Roslaili; Kurniawati, Yulia
SINERGI : Jurnal Riset Ilmiah Vol. 2 No. 4 (2025): SINERGI : Jurnal Riset Ilmiah, April 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/sinergi.v2i4.1109

Abstract

Disinfection is one of the preventive program in preventing the growth of bacteria in hospitals such as Staphylococcus aureus and Escherichia coli which are bacteria that cause nosocomial infections, but the use of disinfectants as antibacterials must be in accordance with the right dose and frequency to prevent the development of antibacterial resistance (AMR). This study aims to determine the effectiveness of the disinfectant used to clean the polyclinic floor of RSUP Dr. M. DJamil Padang against Staphylococcus aureus and Escherichia coli. This type of research is an experiment with purposive sampling technique there samples obtained from the polyclinic of RSUP Dr. M. Djamil Padang as many as 20 samples. Sample analysis using Kirby-bauer method (diffusion disc) with 3 times (Triplo) against 2 types of bacteria, Staphylococcus aureus and Escherichia coli to determine the effectiveness of the inhibition of disinfectant. The results showed that the disinfectant used had different inhibitory values against Staphylococcus aureus and Escherichia coli bacteria. The disinfectant has a weak effectiveness against Staphylococcus aureus bacteria with an average diameter of inhibitory obtained of 13.21 mm with the highest value of 15.33 mm and the lowest value of 11.95 mm. The results showed that Escherichia coli bacteria were resistant to the disinfectant used, because there was no inhibitory activity produced by the disinfectant, so the use of the disinfectant was not very effective in inhibiting the growth of Escherichia coli bacteria. The conclusion of this study is the disinfectant used by RSUP Dr. M. Djamil Padang to clean the polyclinic floor has a sensitivity that is less effective in inhibiting the growth of Staphylococcus aureus bacteria, while Escherichia coli bacteria have significant resistance to this disinfectants. So it is recommended to RSUP Dr. M. Djamil Padang to review of the disinfectant used
PERBEDAAN JUMLAH KOLONI BAKTERI PATOGEN PADA GAGANG PINTU BERDASARKAN WAKTU, LOKASI, DAN RUANGAN DI RUANG INTENSIF RSUP DR. M. DJAMIL PADANG Faisaelia, Rezka Nanda; Rasyid, Roslaili; Sulastri, Delmi; Linosefa, Linosefa; Hardisman, Hardisman; Hasmiwati, Hasmiwati
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 3 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, September 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i3.1842

Abstract

Objective: This study aims to determine differences in pathogenic bacterial contamination on door handles based on time, location, and room in the intensive care unit of Dr. M. Djamil Hospital; Methods: This type of research is observational analytic. Samples were taken in 5 intensive rooms with a total of 40 samples. After that, the number of colonies was calculated based on differences in sampling time, door handle location, and room; Results: The results showed that 87.5% of door handles in the intensive care unit of Dr. M. Djamil Hospital were contaminated with bacteria. The bacteria found were S.warneri, S.hom.hominis, A.viridans, S.vitulinus, and S.maltophilia; Conclusion: There is no significant difference in the number of bacterial colonies on the door handles of the intensive care unit of Dr. M. Djamil Hospital based on time, location, and room.
Enhanced expression of L-asparaginase II by fusion of pSSPM3 synthetic promoter into pET-28a+ expression vector for alternative targeted therapy of acute lymphoblastic leukaemia Aliya, Lisana Shiddiqin; Julizar, Julizar; Rasyid, Roslaili; Pertiwi, Dian; Syukriani, Lily; Nursyafi, Fauzan Syarif; Saibi, Ihsan R. A.; Hasibuan, Imron Martua; Febiona, Keysha Putri; Jamsari, Jamsari
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.72304

Abstract

The use of commercial L-asparaginase II from E. coli and Erwinia chrysanthemi for acute lymphoblastic leukemia (ALL) therapy causes adverse effects (allergic reactions, neurotoxicity), necessitating safer alternatives. Serratia plymuthica UBCF_13, harboring the ansB gene, offers a promising source. This study constructed the recombinant plasmid pET-28a+:pSSPM3:ansB to enhance L-asparaginase II expression in E. coli using the synthetic promoter pSSPM3. Molecular verification confirmed successful steps: promoter fusion (674 bp band), ansB digestion (1,057 bp), gene insertion (1,682 bp), and BL21 transformation. Critically, enzyme activity assays revealed that pSSPM3 did not enhance expression in BL21 (0.519 U/mL), showing significantly lower activity (p<0.05) than native controls (0.621 U/mL) and DH10B transformants (0.636 U/mL). While the functional plasmid establishes a platform for novel enzyme production, the unexpected activity reduction in BL21 and higher yield in DH10B highlight host-promoter compatibility challenges. Further optimization of expression systems, purification protocols, and preclinical validation (cytotoxicity, allergenicity) are essential to advance this recombinant enzyme toward therapeutic and scalable industrial applications for ALL in resource-limited settings.
Co-Authors Aliya, Lisana Shiddiqin Andani Eka Putra Angga Kesumah Arni Amir Arni Amir Asri, Rizkia Chairani Asterina, Asterina Astiena, Adila Kasni Astri Sentyaningrum Ayu Andrian Putri Delmi Sulastri Dewi Oktavia Djasmi Dian Pertiwi Edison Edison Edison Edison Eliza Anas Elizabeth Bahar Ellyza Nasrul Elmatris Syamsir Elsi Wineri Endrinaldi Erlina Rustam Ervan Arditya Kusuma Faisaelia, Rezka Nanda Febiona, Keysha Putri Fitra Dini Genta Pradana Hardisman Hasibuan, Imron Martua Hasmiwati Jamsari Jamsari Julizar Julizar Lily Restusari Lily Syukriani Linosefa Linosefa M. Fadila Arie Novard Muhamad Fadil Muhammad Hidayat Muhammad Reza Nelmi Silvia Nelvita Sari Ramadhan Netti Suharti Netti Suharti Novilla Rezka Sjahjadi Nugroho, Faisal Nursyafi, Fauzan Syarif Qonita Syafrina Raden Mohamad Herdian Bhakti Rahmatini . Rima Semiarty Rini Rustini Rizanda Machmud Rosfita Rasyid Rumaisha, Rumaisha Saibi, Ihsan R. A. Satya Wydya Yenny Sugma, Lidya Aprilia Sukri Rahman Ulya Uti Fasrini Yanwirasti Yanwirasti Yonna Yolanda Y Yuniar Lestari Yustini Alioes Zulkarnain, Anugrah