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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Biotika: Jurnal Ilmiah Biologi Biotropika JURNAL ISTEK Pro-Life Kumawula: Jurnal Pengabdian Kepada Masyarakat KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Indonesian Journal of Limnology Farmers: Journal of Community Services
Nining Ratningsih
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Social Sciences Veterinary Other

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Journal : Pro-Life

 1 
POTENSI REGENERASI SEL SERTOLI DAN SEL LEYDIG TIKUS (Rattus norvegicus) MODEL DIABETES PASCA PEMBERIAN EKSTRAK ETANOL KULIT BUAH JENGKOL (Archidendron pauciflorum) Desak Made Malini; Nining Ratningsih; Nurullia Fitriani; Dwi Rahmi
Jurnal Pro-Life Vol. 7 No. 2 (2020): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Diabetes mellitus (DM) is a metabolic disease that causes disruption of spermatogenesis due to decreased numbers of Sertoli cells and Leydig cells. The aim of this study was to determine the potency of ethanol extract of Jengkol fruit peel (JFPEE) on increasing the regeneration of Leydig cells and Sertoli cells in diabetic rat models. This type of research is experimental research using a completely randomized design (CRD)with 5 treatments and 5 replications. Treatment was given orally for 54 consecutive days consisting of negative control (NC), positive control (PC), comparison (Glibenclamide dose 10 mg/kg BW), P1 and P2 (JFPEE dose 385 and 770 mg/kg BW). Diabetic induction was performed with streptozotocin dose 65 mg/kg BW in male Wistar rat except for NC group. The parameters observed were the number of Sertoli cells and Leydig cells in 25 seminiferous tubules.The results of histological structured showed that the highest number of Sertoly cells and Leydig cells were obtained in group P2 (4.40±0.55; 9.80±0.84) and it was not significantly different from the NC group (5.00±1.41; 12.20±2.77). It can be concluded that 770 mg/kg BW was the effective dose of JFPEE that can increase the regeneration of Sertoli cells and Leydig cells in diabetic rat models.Keywords: Jengkol Fruit Peel Ethanol Extract, Leydig Cells, Regeneration, Sertoli Cells.
ANALISIS PERTUMBUHAN DAN KANDUNGAN TOTAL FLAVONOID KULTUR KALUS KRISAN (Chrysanthemum morifolium Ramat) DENGAN PEMBERIAN ASAM 2,4-DIKLOROFENOKSIASETAT (2,4-D) DAN AIR KELAPA Tia Setiawati; Anggita Levi Astuti; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 1 (2021): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Chrysanthemum morifolium contains flavonoids which have antioxidant properties and was useful in treating various diseases. The production of flavonoids from plants can be done in vitro through callus culture. Auxin 2,4-D growth regulators and organic compounds such as coconut water added to the media can affect the success of callus formation and secondary metabolite synthesis. The purpose of this study was to obtain the best treatment of 2,4-D concentration and coconut water that can increase the growth and total flavonoid levels of C. morifolium callus cultures. The method used in this research was the experimental method completely randomized design (CRD) with 8 treatments, namely 1 ppm 2,4-D + 10% coconut water (CW); 2 ppm 2,4-D + 10% CW; 3 ppm 2,4-D + 10% CW ; 4 ppm 2,4-D + 10% CW; 1 ppm 2,4-D; 2 ppm 2,4-D; 3 ppm 2,4-D; 4 ppm 2,4-D in 4 replication. The results showed that the treatment of 4 ppm 2,4-D and 10% CW was the best treatment combination, resulting in an average callus size (1.4 cm), fresh weight (0.19 grams), dry weight (0.16 gram) and total flavonoid content (1.873 mgQE/g)
Pemberian Asam Humat untuk Meningkatkan Pertumbuhan dan Kandungan Total Flavonoid Sawi Hijau (Brassica juncea L.) pada Perbedaan Kadar Salinitas Tia Setiawati; Tentani Buhti Amadea; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 3 (2021): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Salt affects plant growth due to increased soil osmotic pressure and plant nutrients disturbance. Most plants are susceptible to high salinity which can cause physiological and biochemical interference. This research was conducted to obtain a possible salinity level for mustard greens (Brassica juncea L.) to grow well and the best humic acid dosage to reduce salinity damages and to gain high total flavonoid content. This research used Randomized Block Design method with two factors and four replications. The factors are salinity levels (control, 50, 75, and 100 mM) and humic acid dosage (control, 2, 4, and 8 g/kg). In the parameters which had interactions between the two treatments, the highest results were obtained with a combination of 100 mM salinity and 12 g humic acid, such as plant height (33.35 cm), leaf area (379.66 cm2), fresh weight (22.41 g) and dry weight (1.54 g). The highest results on leaf number (8.5 leaves) and total flavonoid content (0.074 mgQE/g sample) were obtained with 100 mM salinity. Humic acid dose of 12 g gave the highest result on leaf number (8.69 leaves) and total flavonoid content (0.095 mgQE/g sample).
Co-Authors Ade Pandi Adnin Laila Alyssa Meutia Fadiyah Andry Dian Alyssa Anggita Levi Astuti Annisa Anindyta Larasati Annisa Annisa Annisa Annisa Azalea Putri Dea Rahman Khairunnisa Desak Made Malini Dini Tasuruni Dita Aprila Khoirunnisa Dwi Rahmi Dwiki Danang Kuncoro Dyah Retnosari Emay Maulani Erri Noviar Megantara Ida Indrawati Indri Wulandari Indriani Sasmita Kabul Fadilah Kartiawati Alipin Keukeu Kaniawati Rosada Madihah Madihah Mia Miranti Mia Miranti Miranti Ariyani Mohamad Nurzaman Nadia Istiqomah Nia Daniati Hidajat Nurullia Fitriani Retnaningtyas Siska Dianty Rini Hafzari Rusdi Hasan Sadiah Djajasupena Salsabila Nurjihan Sri Rejeki Rahayuningsih SRI WULANDARI sunardi sunardi Supartini Syarif Supartini Syarif Suryana Suryana Sya Sya Shanida Syifa Yolanda Tatang Suharmana Erawan Teguh Husodo Tentani Buhti Amadea Tia Setiawati Titin Supriatun Tresna Wardhana Wawan Hermawan Yetty Yusri Gani Yolani Syaputri Yoyo Marten