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Journal : Microbiology Indonesia

Orchid Mycorrhizal Fungi: Identification of Rhizoctonia from West Kalimantan ROSA SURYANTINI; REINE SUCI WULANDARI; RINA SRI KASIAMDARI
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2256.142 KB) | DOI: 10.5454/mi.9.4.3

Abstract

Orchid is an ornamental plants with high economic value. The excessive exploitation of orchids threatened or even endangered the species, especially those of the epiphytic orchids (Appendiculla sp., Calanthe vestita, and Bulbophyllum beccarii) in West Kalimantan. The discovery of the interaction between orchids and mycorrhizal fungi raises the possibility of ex situ conservation of orchids and it will ensure the success of orchid conservation. Orchid mycorrhizal fungi belongs to the group of Rhizoctonia-like,in which comprised of different genus such as Ephulorhiza, Ceratoriza, and Tullasnela. So far, there is no report on the identit of orchid mycorrhiza associated with the epiphytic orchids in West Kalimantan. The purpose of this study was to identify Rhizoctonia-like associated with Appendiculata sp., Calanthe vestita, and Bulbophyllum beccarii roots in the forest of Raya Pasi and Gunung Bawang, West Kalimantan. The methods were isolation and identification of Rhizoctonia-like from healthy orchid's root based on their morphological characteristics (such as the colony colour, hyphal cell size, sclerotial, concentric circles and monilioid cell, number of nuclei per cell), observation of peloton in root tissue and grouping of isolates. Based on identification of orchid mycorrhiza on the roots of the three species of orchids from West Kalimantan, it was observed that Ceratorhiza sp. was associated with Appendiculla sp.,Ephuloriza sp. with C. vestita, and Tullasnela sp. with B. beccarii roots, respectively. This result is preliminary information and it is still need to be further studied, especially on the role of Rhizoctonia-liker as orchid mycorrhizal fungi in association with the epiphytic orchid for conservation. 
ENDOPHYTIC FUNGI IN Paraserianthes falcataria: PRODUCTION OF INDOLE ACETIC ACID REINE SUCI WULANDARI; ROSA SURYANTINI
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2614.494 KB) | DOI: 10.5454/mi.13.1.3

Abstract

Identification of endophytic fungi in Paraserianthes falcatria is the effort of the potential of endophytic fungi as phytohormone producer.  Phytohormone is needed to spur shoot and root initiation.  This study in P. falcatariais necessary when woody of P. falcataria decreases every year.  The aimed of the study were to identify endophytic fungi from leaves, twigs, and roots of P. falcataria, and determine IAA content from endophytic fungi.  Isolates that were grown from leaves, twigs and roots cuttings on PDA, were identified based on micro- and macromorphology. Determining of IAA content was counted with spectrophotometer vis based on a calibration curve from the standard solution.  The results were obtained 10 of isolates fungi from leaves, twigs, and roots.  But from 10, only nine isolates that could be identified.  They were Aspergillus sp., Acremonium sp., Cladosporium sp., Trichoderma sp. 1, Phytium sp., Rhizoctonia sp., Trichoderma sp. 2, Hormiscium sp. 1 and Hormiscium sp. 2.  Production of indole acetic acid (IAA) from Cladosporium sp. had the highest content than others (311 ppm).  The lowest IAA content (51.97 ppm) was produced by the Rhizoctonia sp.  The study can be continued to find out their abilities as PGPF agents and biopesticides of P. falcataria seedlings.