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All Journal METAMORFOSA Journal of Biological Sciences INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES E-Jurnal Medika Udayana Jurnal Veteriner Jurnal e-Biomedik JURNAL BIOMEDIK Prosiding Seminar Nasional MIPA Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya WMJ (Warmadewa Medical Journal) Molecular and Cellular Biomedical Sciences (MCBS) Berkala Ilmiah Kedokteran Duta Wacana ISM (Intisari Sains Medis) : Jurnal Kedokteran Majalah Kesehatan Pharmamedika Jurnal Kedokteran: Media Informasi Ilmu Kedokteran dan Kesehatan Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Neurologico Spinale Medico Chirurgico Indonesian Journal of Global Health research IJAAM (Indonesian Journal of Anti-Aging Medicine) IJCNP (INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN) Indonesian Archives of Biomedical Research (InABR) Cermin Dunia Kedokteran Eduvest - Journal of Universal Studies Asian Journal of Social and Humanities Cermin Dunia Kedokteran
Wimpie I Pangkahila
Department Of Andrologi And Sexology, Faculty Of Medicine, Udayana University
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Krim ekstrak biji markisa (Pasiflora edulis) sama efektifnya dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin pada kulit marmut jantan (Cavia porcelus) yang dipapar sinar UV-B Huda, Siti M. N.; Wiraguna, A A G P; Pangkahila, Wimpie
Jurnal Biomedik : JBM Vol 9, No 1 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.1.2017.15312

Abstract

Abstract: Ultraviolet B (UVB) ray may cause hyperpigmentation of the skin due to increased melanin level. Passion fruit (Passiflora edulis) is a plant traditionally used to brighten the skin and can give satisfactory result due to its natural tyrosinase and antioxidant activities. This study was aimed to prove that cream of passion fruit extract could prevent the increase of skin melanin in UVB-exposed guinea pig (Cavia porcellus). This was an experimental study using a completely randomized posttest only control group design. Subjects were 30 brown guinea pigs, male, adult (6-8 months old), body weight of 250-300 g, divided into 3 groups as follow: 1) P0, the negative control group, exposed to ultraviolet B and applied with cream based substance; P1, the positive control group, exposed to ultraviolet B and applied with 4% hydroquinone cream; and P2, the treatment group, exposed to ultraviolet B and applied 75% passion fruit extract cream. The results showed that the average amount of melanin in the P0 group was 17.52±4.72%, whereas in the P1 group was 1.25±0.45%, and in the P2 group was 1.79±0.78% (P <0.001). The LSD test showed that the P0 group had the highest amount of melanin (P <0.01) meanwhile the P1 group had the similar result with the P2 group (P >0.05). Conclusion: Passion fruit (Passiflora edulis) extract crream could prevent increased skin melanin in UVB-exposed guinea pig (Cavia porcellus) and its effectiveness was similar to those of 4% hydroquinone cream.Keywords: passion fruit, melanin, UVB, guinea pigsAbstrak: Paparan sinar ultraviolet B (UVB) mengakibatkan terjadinya kelainan hiperpigmentasi yang ditandai dengan peningkatan jumlah melanin. Markisa (Passiflora edulis) secara tradisional digunakan untuk mencerahkan kulit dan dapat memberikan hasil yang memuaskan karena memiliki aktivitas tirosinase dan antioksidan alamiah. Penelitian ini bertujuan untuk membuktikan bahwa krim ekstrak markisa dapat mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar UVB. Jenis penelitian ialah eksperimental menggunakan completely randomized posttest only control group design. Subyek ialah 30 ekor marmut jantan, berwarna coklat, dewasa (usia 6-8 bulan), berat badan 250-300 gr, yang dibagi menjadi 3 (tiga) kelompok masing-masing berjumlah 10 ekor marmut. Kelompok P0 (kelompok kontrol negatif) diberikan penyinaran ultraviolet B dan bahan dasar krim. Kelompok P1 (kelompok kontrol positif) diberikan penyinaran ultraviolet B dan krim hidrokuinon 4%. Kelompok P2 (kelompok perlakuan) diberikan penyinaran ultraviolet B dan krim ekstrak markisa 75%. Hasil penelitian mendapatkan rerata jumlah melanin pada kelompok P0 ialah 17,52±4,72%; pada kelompok P1 1,25±0,45%, sedangkan pada kelompok P2 1,79±0,78%. Uji LSD menunjukkan bahwa kelompok P0 memiliki jumlah melanin yang paling tinggi (P <0,01), sedangkan kelompok P1 memiliki jumlah melanin yang tidak berbeda nyata dengan kelompok P2 (P >0,05). Simpulan: Krim ekstrak markisa (Passiflora edulis) dapat mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar UVB dengan efektifitas yang tidak berbeda dengan krim hidrokuinon 4%.Kata kunci: markisa, melanin, UVB, marmut
Pemberian suplemen Swissoats A3® meningkatkan kadar hormon estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar Widhayanthi, Trisna A.; Pangkahila, Wimpie; Pinatih, Gde Ngurah Indraguna
Jurnal Biomedik : JBM Vol 9, No 2 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.2.2017.16354

Abstract

Abstract: Aging is a physiological process that occurs in all living creatures. Swissoats A3® is an anti-aging supplement that contains phytoestrogens. This study was aimed to prove that Swiss oats A3® supplementation could increase estrogen levels in menopausal female Wistar rats (Rattus norvegicus). This was an experimental study with a completely randomized pretest-posttest control group design. Subjects were 20 menopausal female rats (Rattus norvegicus), Wistar strain, healthy, aged 15-16 months, weighing 200 g, divided into 2 groups: the control group (P0), treated with distilled water for 21 days and the treatment group (P1), treated with Swissoats A3® of 34.2 mg/rat/day for 21 days. Before and after 21 days of treatment, 1 ml venous blood was drawn through the medial canthus of orbital sinus using microcapillary tube and the estrogen levels were meassured by using indirect ELISA method. The results showed that before treatment, the mean estrogen level in P0 group was 14.96±2.0468 pg/ml and in P1 group was 14.21±2.3905 pg/ml (P > 0.05). Meanwhile, after 21-day treatment, the mean estrogen level in P0 group was 15.07±1.8851 pg/ml, whereas in P1 group was 28.33±3.2052 pg/ml (P < 0.01). The analysis showed that the Swissoats A3® could elevate the estrogen levels significantly (P < 0.01) in group P1 (2.3905±14.21 vs 28.33±3.205). Conclusion: Swissoats A3® supplementation could increase the estrogen levels in menopausal female Wistar rats (Rattus norvegicus).Keywords: Swiss oats A3®, estrogen, female Wistar rats, menopauseAbstrak: Penuaan (aging) merupakan suatu proses fisiologik yang dialami oleh seluruh mahluk hidup. Swissoats A3® merupakan suplemen anti-aging yang mengandung fitoestrogen. Penelitian ini bertujuan untuk membuktikan bahwa pemberian suplemen Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar. Jenis penelitian ialah eksperimental dengan randomized pretest-posttest control group design. Subjek penelitian ialah 20 ekor tikus putih betina (Rattus norvegicus) galur Wistar, sehat, menopause, umur 15-16 bulan, berat badan 200 gr. Tikus dibagi menjadi 2 kelompok yaitu kelompok kontrol (P0) yang diberikan aquadest secara sonde selama 21 hari dan kelompok perlakuan yang diberikan suplemen Swissoats A3® dosis 34,2mg/ekor/hari secara sonde selama 21 hari. Sebelum dan sesudah 21 hari perlakuan, darah vena diambil melalui medial canthus sinus orbitalis sekitar 1 ml dengan menggunakan tabung mikrokapiler dan dilakukan pemeriksaan kadar estrogen menggunakan metode indirect ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan, rerata kadar estrogen kelompok P0 14,96±2,0468 pg/ml dan kelompok P1 14,21±2,3905 pg/ml (P > 0,05). Setelah perlakuan selama 21 hari, rerata kadar estrogen kelompok P0 15,07±1,8851 pg/ml dan kelompok P1 28,33±3,2052 pg/ml (P < 0,01). Analisis efek perlakuan menunjukkan terjadinya peningkatan kadar hormon estrogen yang sangat bermakna (P < 0,01) pada kelompok P1 (14,21±2,3905 vs 28,33±3,205). Simpulan: Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur wistar.Kata kunci: Swissoats A3®, estrogen, tikus wistar betina, menopause
KRIM EKSTRAK KULIT DELIMA MERAH (PUNICA GRANATUM) MENGHAMBAT PENINGKATAN JUMLAH MELANIN SAMA EFEKTIFNYA DENGAN KRIM HIDROKUINON PADA KULIT MARMUT (CAVIA PORCELLUS) BETINA YANG DIPAPAR SINAR UVB Siahaan, Eva R.; Pangkahila, Wimpie; Wiraguna, A A G P
JURNAL BIOMEDIK : JBM Vol 9, No 1 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.1.2017.15313

Abstract

Abstract: As the outer organ of the body, skin becomes the main target of environmental influences, particularly the UVB rays. Based on analysis of phytochemicals, red pomegranate peel extract contains antioxidants such as flavonoids, phenols, tannins, and vitamin C which are able to prevent increased skin melanin by inhibiting the actions of tyrosinase. This study was aimed to prove that application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in female guinea pigs (Cavia porcellus) exposed to UVB as well as to prove that the 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin. This was a true experimental study using post test only control group design. Subjects were divided into three groups, with 10 guinea pigs each group. Group 1, the control group, was treated with UVB exposure and basic cream. Group 2 was treated with UVB exposure and 4% hydroquinone cream. Group 3 was treated with UVB and 8% red pomegranate peel extract cream. A total of 390 mJ/cm UVB dosage was given for 2 weeks. Histopahtologic slides were stained with Masson-Fontana. The amount of melanin area colored in black was evaluated by the percentage of pixel areas of melanin compared with pixel areas of all epidermal tissues. The results showed that the highest amount of melanin was in group 1 (18.13±4.76%), followed by group 3 (1.876±0.73%), and group 2 as the lowest (0.67±0.291%). There was a significant difference between control group and group 2 as s well as group 3 (P <0.05). There was no significant difference between group 2 and group 3 (P >0.05). Conclusion: Application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in guinea pigs (Cavia porcellus) exposed to UVB. The 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin.Keywords: red pomegranate peel extract, melanin, skin, UVBAbstrak: Sebagai organ terluar dari tubuh, kulit menjadi target utama dari pengaruh lingkungan, terutama oleh sinar UVB. Berdasarkan analisis fitokimia, ekstrak kulit delima merah memiliki kandungan antioksidan seperti flavonoid fenol, tanin, dan vitamin C yang mampu mencegah peningkatan jumlah melanin dengan cara menghambat kerja enzim tirosinase. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak kulit delima merah (Punica granatum) 8% dalam menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB dan krim ekstrak kulit delima merah (Punica granatum) 8% memiliki efektivitas yang sama dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin tersebut. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian dibagi menjadi tiga kelompok, masing-masing berjumlah 10 ekor marmut betina. Kelompok 1 (kontrol) diberikan pajanan sinar UVB dan krim dasar; kelompok 2 diberikan paparan sinar UVB dan krim hidrokuinon 4%; dan kelompok 3diberikan pajanan sinar UVB dan krim ekstrak kulit delima merah 8%. Dosis total UVB 390 mJ/cm2 yang diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit menggunakan pewarnaan Masson-Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan bahwa jumlah melanin paling tinggi terdapat pada kelompok 1 (18,13±4,76%), diikuti kelompok 3 (1,876±0,73%), dan paling rendah pada kelompok 2 (0,67±0,291%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05) sedangkan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak kulit delima merah (Punica granatum) 8% dapat menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina. Krim ekstrak kulit delima merah (Punica granatum) 8% sama efektif dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin pada kulit marmut.Kata kunci: kulit delima merah, melanin, kulit, UVB, marmut betina.
PEMBERIAN EKSTRAK DAUN GANDARUSA (JUSTICIA GENDARUSSA BURM. F.) MENURUNKAN KADAR F2-ISOPROSTAN URIN TIKUS WISTAR (RATTUS NORVEGICUS) JANTAN YANG DIINDUKSI LATIHAN FISIK BERLEBIH Akpriyanti, DG Ika; Pangkahila, Wimpie; Aman, IGM
JURNAL BIOMEDIK : JBM Vol 9, No 3 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.3.2017.17337

Abstract

Abstract: Excessive physical activity increases the consumption of oxygen which can result in increased levels of reactive oxygen species (ROS) causing oxidative stress characterized by the elevation of F2-isoprostane as a byproduct of lipid peroxidation. Willow (Justicia gendarussa Burm. f.) has an antioxidant activity that is able to inhibit and break the chain reaction of free radicals and prevent lipid peroxidation. This study was aimed to prove that willow leaf extract could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training. This was a true experimental study using 16 male Wistar rats (Rattus norvegicus), aged 2-3 months, weighing 180-200 g as subjects. The control group (P0) was treated with a placebo and excessive physical training, and the treatment group (P1) was treated with the willow leaf extract of 200mg/rat/day and excessive physical training. Before and after 14 days of treatment, the urine samples were collected and their levels of F2-isoprostane 8-isoPGF2? were examined by using enzyme immunoassay kit (EIA). The results showed that the mean level of F2-isoprostane in the control group (P0) before treatment (pretest) was 5.20±0.483 ng / mL while in the treatment group (P1) was 5.46±0.655 ng / mL (P > 0.05). On the other hand, after treatment for 7 days (post-test), the mean level of F2-isoprostane in the control group (P0) was 5.34±0.476 ng/mL and in the treatment group (P1) was 3.61±0.389 ng/mL (P < 0.01). Conclusion: The extract of willow (Justicia gendarussa Burm. f.) leaf could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training.Keywords: willow leaf, F2-isoprostane, excessive physical trainingAbstrak: Aktivitas fisik berlebih akan meningkatkan komsumsi oksigen yang dapat mengakibatkan peningkatan kadar reactive oxygen species (ROS) sehingga menyebabkan stres oksidatif yang ditandai dengan meningkatnya F2-isoprostan sebagai salah satu produk peroksidasi lipid. Tanaman gandarusa (Justicia gendarussa Burm. f.) mengandung antioksidan yang dapat mencegah reaksi peroksidasi lipid. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun gandarusa dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih. Jenis penelitian ialah eksperimental dengan subjek 16 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 6 bulan, berat badan 180-200 gr, dengan kadar F2-isoprostan >2ng/mL. Kelompok kontrol (P0) diberikan pelatihan fisik berlebih dan plasebo aquadest 2ml sedangkan kelompok perlakuan (P1) diberikan pelatihan fisik berlebih dan ekstrak daun gandarusa dosis 200mg/ekor/hari. Sebelum dan setelah 14 hari perlakuan, sampel urin ditampung dan diperiksa kadar F2-isoprostan menggunakan 8-isoPGF2? enzyme immunoassay kit (EIA) dari assay design untuk data pretest dan post-test. Hasil penelitian menunjukkan rerata kadar F2-isoprostan pada kelompok P0 sebelum perlakuan (pretest) ialah 5,20±0,483 ng/mL, sedangkan pada kelompokP1 ialah 5,46±0,655 ng/mL (P > 0,05). Setelah perlakuan selama 14 hari (post-test), rerata kadar F2-isoprostan pada kelompok P0 ialah 5,34±0,476 ng/mL dan pada kelompok P1 ialah 3,61±0,389 ng/mL (P < 0,01). Simpulan: Ekstrak daun gandarusa (Justicia gendarussa burm. f.) dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih.Kata kunci: daun gandarusa, F2-isoprostan, pelatihan fisik berlebih
PEMBERIAN BISPHENOL A (BPA) ORAL DAPAT MENURUNKAN KADAR TESTOSTERON PADA TIKUS (RATTUS NORVEGICUS) JANTAN GALUR SPRAGUE DAWLEY ., Nuriyah; Aman, IGM; Pangkahila, Wimpie
JURNAL BIOMEDIK : JBM Vol 9, No 2 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.2.2017.16355

Abstract

Abstract: Bisphenol A (BPA) has the structural and physiological activity of estrogen and acts as an endocrine disruptor. This study was aimed to prove that oral BPA could decrease testosterone levels in male Sprague Dawley rats. This was an experimental study with a randomized pretest-posttest control group design using 14 rats (Rattus norvegicus), male, Sprague Dawley strain, 2-3 months old, as subjects. Rats were divided into two groups with 7 rats each. The control group (P0) was treated with placebo for 14 days and the treatment group (P1) was treated with oral bisphenol A (BPA) single dose of 400mg/kg/day for 14 days (P1). At before and after 14-day treatment, blood was drawn through the canthus medial of orbital sinus to obtain the pretest and posttest data. Testosterone levels were examined by using ELISA. The results showed that before treatment (pretest), the mean testosterone level in P0 group was 8.29±4.68 nmol/L, while in P1 group was 7.39±1.45 nmol/L (P > 0.05). After treatment for 14 days, the mean testosterone level in P0 group was 7.40±1.37 nmol/L, while in P1 group was 3.36±1.26 nmol/L (P < 0.01). The statistical analysis showed that there was no difference in testosterone levels in P0 group (P > 0.05), meanwhile there was a very significant decrease of testosterone levels in P1 group (P < 0.01) after the treatment of BPA 400mg/kg/day for 14 days. Conclusion: Oral bisphenol A (BPA) could decrease testosterone levels in male rats (Rattus norvegicus) of Sprague Dawley strain.Keywords: bisphenol A (BPA), testosteroneAbstrak: Bisphenol A (BPA) memiliki aktifitas hormon estrogen sehingga jika masuk ke dalam tubuh dapat menjadi endocrine disruptor. Penelitian ini bertujuan untuk membuktikan pemberian BPA oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley. Jenis penelitian ialah eksperimental dengan randomized pretest-postest control group design yang menggunakan 14 ekor tikus (Rattus norvegicus) jantan, galur Sprague Dawley, berumur 2-3 bulan, yang terbagi menjadi 2 (dua) kelompok masing-masing berjumlah 7 ekor tikus. Kelompok kontrol (P0) diberikan plasebo 14 hari dan kelompok perlakuan (P1) diberi BPA dosis tunggal 400mg/kgBB/hari selama 14 hari. Saat sebelum dan sesudah perlakuan selama 14 hari, darah diambil melalui chantus medial orbitalis untuk pemeriksaan pretest dan postest dan kadar testosteron diperiksa dengan metode ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan (pretest), rerata kadar testosteron kelompok P0 8,29±4,68 nmol/L, sedangkan pada kelompok P1 7,39±1,45 nmol/L (P > 0,05). Setelah perlakuan selama 14 hari, rerata kadar testosteron kelompok P0 ialah 7,40±1,37 nmol/L dan pada kelompok P1 3,36±1,26 nmol/L (P < 0,01). Analisis efek perlakuan menunjukkan tidak terjadi penurunan kadar hormon testosteron pada kelompok P0 (P > 0,05), sedangkan penurunan bermakna terdapat pada kelompok P1 (P < 0,01). Simpulan: Pemberian bisphenol A (BPA) oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley.Kata kunci: bisphenol A (BPA), testosteron
Pemberian alpha lipoic acid per oral dan latihan fisik intensitas sedang menurunkan berat badan dan lemak abodminal lebih banyak daripada latihan fisik intensitas sedang saja pada tikus Wistar jantan dengan obesitas Chandra, Aji B.; Pangkahila, Alex; Pangkahila, Wimpie
Jurnal Biomedik : JBM Vol 9, No 1 (2017): JURNAL BIOMEDIK : JBM Suplemen
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.1.2017.15377

Abstract

Abstract: This study was aimed to prove that oral administration of ALA and moderate physical exercise decrease body weight and abdominal fat more than moderate physical exercise only in obese male Wistar rats. This was a true experimental study with a post-test only control group design. Subjects were 30 Wistar rats (Rattus norvegicus), male, healthy, aged 4-5 months, and obese with minimum weight of 250 g, divided into 3 groups, as follows: 1) P0, the control group with no treatment; 2) P1, given moderate physical exercise (swimming for 20 minutes/day, for 4 weeks); 3) P2, given moderate physical exercise and ALA per oral 15 mg/day for 4 weeks. The results showed that the average body weight after 4 weeks of treatment of P0 group was 279.10±5.84 g; of P1 group was 257.90±10.31 g; and of P2 group was 213.90±8.92 g (P < 0.01). The mean weight of subcutaneous abdominal fat of P0 group was 0.96±0.45 g; of P1 group was 0.63±0.31 g; and of P2 group was 0.40±0.23 g (P < 0.01 ). Moreover, the mean weight of visceral abdominal fat of P0 group was 1.23±0.37 g; of P1 group was 0.83±0.24 g; and of P2 group was 0.39±0.25 g (P < 0,01). Conclusion: Oral administration of ALA and moderate physical exercise decrease body weight, subcutaneous abdominal fat, and visceral abdominal fat more than moderate physical exercise only in obese male Wistar rats.Keywords: alpha lipoic acid, obesity, body weight, abdominal fatAbstrak: Penelitian ini bertujuan untuk membuktikan bahwa pemberian ALA per oral yang dikombinasi dengan latihan fisik intensitas sedang menurunkan berat badan, lemak subkutan abdominal, dan lemak viseral abdominal lebih banyak daripada latihan fisik intensitas sedang saja pada tikus Wistar jantan dengan obesitas. Jenis penelitian ialah eksperimental murni dengan post-test only control group design. Subjek penelitian ialah 30 ekor tikus putih (Rattus norvegicus) jantan, galur Wistar, sehat, usia 4-5 bulan, dan obes dengan berat badan minimal 250 g yang terbagi menjadi tiga kelompok masing-masing berjumlah 10 ekor tikus, yaitu: P0 (kelompok kontrol), tidak mendapat perlakuan apapun; P1, kelompok perlakuan 1 yang diberikan latihan fisik intensitas sedang (renang durasi 20 menit/hari selama 4 minggu); dan P2, kelompok perlakuan 2 yang diberikan latihan fisik intensitas sedang serta ALA per oral dengan dosis 15mg/hari selama 4 minggu. Hasil penelitian menunjukkan bahwa rerata berat badan setelah 4 minggu perlakuan pada kelompok P0 ialah 279,10±5,84 gr; pada kelompok P1 257,90±10,31 gr; dan pada kelompok P2 213,90±8,92 gr (P < 0,01). Rerata berat lemak subkutan abdominal pada kelompok P0 ialah 0,96±0,45 gr; pada kelompok P1 0,63±0,31 gr; dan pada kelompok P2 0,40±0,23 gr (P < 0,01). Selain itu, rerata berat lemak viseral abdominal pada kelompok P0 ialah 1,23±0,37 gr; pada kelompok P1 0,83±0,24 gr; dan pada kelompok P2 0,39±0,25 gr (P < 0,01). Simpulan: Pemberian ALA per oral dengan latihan fisik intensitas sedang dapat menurunkan berat badan, lemak subkutan abdominal, dan lemak viseral abdominal lebih banyak daripada latihan fisik intensitas sedang saja pada tikus Wistar jantan dengan obesitasKata kunci: alpha lipoic acid, obesitas, berat badan, lemak abdominal
Injeksi asam hialuronat di lapisan dermis menghambat peningkatan ekspresi MMP-1 tikus yang dipajan sinar ultraviolet-B (UVB) Tamon, Oktavian; Wiraguna, A A G P; Pangkahila, Wimpie
Jurnal Biomedik : JBM Vol 9, No 1 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.1.2017.15315

Abstract

Abstract: Ultraviolet B (UVB) is a source of free radicals that accelerate aging process, especially in the skin, through the increase of MMP-1. Hyaluronic acid contained in the dermal filler injection may provide a protective effect against skin exposure to UV rays. This study was aimed to prove that intradermal injection of hyaluronic acid could prevent the increase of MMP-1 in UVB-induced rats (Rattus norvegicus). This was a true experimental study with the post test only control group design. Subjects were 30 male Wistar rats (Rattus norvegicus), aged 2,5-3 months, weighing 160-180 g, and divided into 3 groups. The control group (P0) was exposed to UV-B only; the treatment group 1 (P1) was exposed to UVB and treated with placebo (aquadest intradermal injection); and the treatment group 2 (P2) was exposed to UVB and treated with hyaluronic acid intradermal injection. After 15 days of treatment, all rats were anesthetized and their skin tissues were prepared for examination of MMP1 levels. The statistic analysis showed that the average level of MMP1 in the P0 group was 24.54±4.39%; in the P1 group was 21.35±2.48% (P < 0.01). The average level of MMP-1 in P2 group was 15.40±3.87%, and was statistically lower than P0 group (P < 0.01) and P1 group (P < 0.01). Conclusion: Injection of hyaluronic acid in the dermal layer could prevent the increase of MMP-1 in UVB-induced rats (Rattus norvegicus).Keywords: hyaluronic acid, MMP-1, UVBAbstrak: Ultraviolet B (UVB) merupakan salah satu sumber radikal bebas yang dapat mempercepat proses penuaan, khususnya penuaan pada kulit melalui peningkatan MMP-1. Asam hialuronat yang terkandung dalam dermal filler injection dapat memberi efek perlindungan kulit terhadap pajanan sinar ultraviolet. Tujuan penelitian ini untuk membuktikan bahwa pemberian asam hialuronat intradermal menghambat peningkatan ekspresi MMP-1 tikus yang dipapar sinar UVB. Jenis penelitian ialah eksperimental murni menggunakan post test only control group design. Subjek penelitian ialah 30 ekor tikus putih (Rattus norvegicus), jantan, galur Wistar, berumur 2,5-3 bulan, dengan berat badan 160-180 gram yang terbagi menjadi 3 kelompok masing-masing berjumlah 10 ekor tikus. Kelompok kontrol (P0) hanya diberi pajanan UVB; kelompok plasebo (P1) diberikan injeksi aquabidest intradermal serta pajanan UVB; dan kelompok perlakuan (P2) diberikan injeksi asam hialuronat intradermal dan pajanan UVB. Setelah 15 hari perlakuan, seluruh tikus dianestesi kemudian jaringan kulitnya diambil untuk pemeriksaan ekspresi MMP-1 dermis. Hasil analisis statistik menunjukkan bahwa rerata ekspresi MMP-1 pada kelompok P0 ialah 24,54±4,39% dan pada kelompok P1 21,35±2,48% (P > 0,05). Pada kelompok P2, rerata ekspresi MMP-1 ialah 15,40±3,87%, yang secara statistik lebih rendah dibandingkan dengan kelompok P0 (P < 0,01) dan kelompok P1 (P < 0,01). Simpulan: Pemberian asam hialuronat intradermal menghambat peningkatan ekspresi MMP-1 tikus yang dipapar sinar UVB.Kata kunci: asam hialuronat, MMP-1, UVB
Pelatihan Fisik Berlebih Menurunkan Jumlah Hematopoietic Stem Cells (HSCs) Dibandingkan Pelatihan Fisik Seimbang pada Tikus (Rattus norvegicus) Wistar Jantan ., Zenitalia; Pangkahila, Alex; Pangkahila, Wimpie; Siswanto, Ferbian M.
Jurnal Biomedik : JBM Vol 10, No 1 (2018): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.10.1.2018.18996

Abstract

Abstract: This study was aimed to prove that balanced physical exercise increased mobilization of hematopoietic stem cells (HSCs) compared to excessive physical exercise in male Wistar rats (Rattus norvegicus). This was an experimental study with the randomized pretest-posttest control group design. Subjects were 24 male Wistar rats (Rattus norvegicus), 2.5-3 months old, weighing 180-200 g, healthy (active and did not show any anatomical abnormality), divided into two groups of 12 rats each. One group (P0) was treated with balanced physical exercise and the other group (P1) was treated with excessive physical exercise. Before (pretest) and 4 weeks after treatment (posttest), peripheral blood of 1 ml was drawn through medial canthus sinus orbitalis for examination of HSCs number in peripheral blood quantitatively. The results showed that the number of HSCs in the P0 group was increased from 1.60±0.70 x 106 cell/μl before treatment to 2.70±0.62 x 106 cells/μl after 4-week treatment (P <0.05). Meanwhile, in the P1 group, the number of HSCs was decreased from 1.74±0.68 x 106 cells/μl before treatment to 1.34±0.55 x 106 cells/μl after 4-week treatment (P <0.05). Conclusion: Excessive physical exercise decreased number of hematopoietic stem cells (HSCs) compared to balanced physical exercise in male Wistar rats (Rattus norvegicus).Keywords: balanced physical exercise, excessive physical exercise, hematopoietic stem cells (HSCs)Abstrak: Tujuan penelitian ini ialah untuk membuktikan bahwa pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan. Jenis penelitian ini ialah eksperimental dengan randomized pre-post test control group design. Subyek penelitian ialah 24 ekor tikus Wistar (Rattus norvegicus) jantan, umur 2,5-3 bulan, berat badan 180-200 gr, sehat (aktif dan tidak menunjukkan abnormalitas anatomi) yang dibagi menjadi dua kelompok yaitu P0 dan P1, masing-masing berjumlah 12 ekor. Kelompok P0 diberikan pelatihan seimbang dan kelompok P1 diberi pelatihan fisik berlebih. Sebelum (pretest) dan 4 minggu setelah perlakuan (posttest), darah diambil sebanyak 1 ml melalui medial canthus sinus orbitalis untuk pemeriksaan kadar HSCs darah tepi secara kuantitatif. Hasil penelitian menunjukkan bahwa pada kelompok P0 terjadi peningkatan jumlah HSCs dari 1,60±0,70 x106 sel/μl pretest menjadi 2,70±0,62 x106 sel/μl posttest (P <0,05). Pada kelompok P1 terjadi penurunan jumlah HSCs dari 1,74±0,68 x106 sel/μl pretest menjadi 1,34±0,55 x106 sel/μl posttest (P <0,05). Simpulan: Pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan.Kata kunci: pelatihan fisik seimbang, pelatihan fisik berlebih, hematopoietic stem cells (HSCs)
Pemberian tempoyak per oral dapat menghambat peningkatan ekspresi matriks metaloproteinase-1 pada tikus (Rattus norvegicus) betina dewasa galur Wistar yang dipajan sinar UVB Fiter, Joni; Wiraguna, AAG Putra; Pangkahila, Wimpie
Jurnal Biomedik : JBM Vol 9, No 2 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.2.2017.16357

Abstract

Abstract: Ultraviolet B (UVB) is a source of free radicals that accelerate aging process, especially in the skin by increasing the expression of MMP-1. Tempoyak is rich in nutraceuticals and probiotics that may provide a protective effect against skin exposure to ultraviolet rays. This study was aimed to prove that oral tempoyak could inhibit the increase of matrix metalloproteinase-1 (MMP-1) in UVB-induced rats (Rattus norvegicus). This was a true experimental study with the posttest only control group design. Subjects were 36 female Wistar rats (Rattus norvegicus), aged 2.5-3 months, weighing180-200 g, divided into 2 groups. The control group (P0) exposed to UVB was given oral aquadest as placebo, while the treatment group (P1) exposed to UVB was given 1 g/200 g body weight of oral tempoyak. After 15 days of treatment, all rats were anesthetized and their skin tissues were taken for examination of MMP-1 expression. The analysis showed that the average of MMP-1 expression in the control group (P0) was 25.26±11.19% meanwhile the average of MMP-1 expression in the treatment group (P1) was 8.67±2.51%. There was a significant difference between the MMP-1 expression of the two groups (P = 0.000). Conclusion: Oral tempoyak could inhibit the increase of matrix metalloproteinase-1 (MMP-1) in UVB-induced rats (Rattus norvegicus).Keywords: tempoyak, MMP-1, UVBAbstrak: Ultraviolet B (UVB) merupakan salah satu sumber radikal bebas yang dapat mempercepat proses penuaan, khususnya penuaan kulit melalui peningkatan ekspresi MMP-1. Tempoyak yang kaya akan kandungan nutraseutikal dan probiotik dapat memberikan efek perlindungan kulit terhadap pajanan sinar ultraviolet. Penelitian ini bertujuan untuk membuktikan bahwa pemberian tempoyak per oral dapat menghambat peningkatan ekspresi MMP-1 tikus yang dipajan sinar ultraviolet-B (UVB). Jenis penelitian ialah eksperimental murni dengan menggunakan post test only control group design. Subjek penelitian ialah 36 ekor tikus putih (Rattus norvegicus), betina, galur Wistar, berumur 2,5-3 bulan, dengan berat badan 180-200 gr yang dibagi menjadi 2 (dua) kelompok masing-masing berjumlah 18 ekor tikus. Kelompok kontrol (P0) diberikan pajanan UVB dan akuades per oral sebagai plasebo sedangkan kelompok perlakuan (P1) diberikan pajanan UVB dan tempoyak dengan dosis 1 gr/ 200 gr BB. Setelah 15 hari perlakuan, seluruh tikus dianestesi kemudian diambil jaringan kulitnya untuk pemeriksaan ekspresi MMP-1 dermis. Hasil analisis menunjukkan bahwa rerata ekspresi MMP-1 pada kelompok kontrol (P0) ialah 25,26±11,19% sedangkan rerata ekspresi MMP-1 pada kelompok perlakuan (P1) ialah 8,67±2,51%. Terdapat perbedaan bermakna antara rerata ekspresi MMP-1 antara kedua kelompok (P = 0,000). Simpulan: Pemberian tempoyak per oral dapat menghambat peningkatan ekspresi MMP-1 tikus (Rattus norvegicus) yang dipajan sinar ultraviolet-B (UVB).Kata kunci: tempoyak, MMP-1, UVB
KRIM EKSTRAK BUAH MERAH (PANDANUS CONOIDEUS ) 10% SAMA EFEKTIFNYA DENGAN KRIM HIDROKUINON 4% DALAM MENCEGAH PENINGKATAN JUMLAH MELANIN KULIT MARMUT (CAVIA PORCELLUS) YANG DIPAPAR SINAR ULTRAVIOLET B Dumaria, Corazon H.; Wiraguna, A AGP; Pangkahila, Wimpie
JURNAL BIOMEDIK : JBM Vol 10, No 2 (2018): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.10.2.2018.20085

Abstract

Abstract: This study was aimed to prove that administration of 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB and to compare this effect with 4% hydroquinone cream. This was a true experimental study using post test only control group design. Subjects were devided into three groups; each of 10 guinea pigs. Group 1, the control group, was exposed to UVB and applied with basic cream. Group 2 was exposed to UVB and applied with 4% hydroquinone cream. Group 3 was exposed to UVB and applied with 10% redfruit extract cream. The total dose of UVB was 390 mJ/cm2 given for 2 weeks. The amount of melanin was calculated using the percentage of the pixel area of melanin and was compared with the pixel areas of all epidermal tissues. The results showed that the highest percentage of melanin area was in group 1 (19.78%±3.79%). The percentage of melanin area in group 3 was 1.25%±0.76% meanwhile in group 2 was 0.85%±0.37%. There were signi-ficant differences in melanin percentage between the control group and group 2 as well as group 3 (P <0.05). There was no significant difference in melanin percentage between group 2 and group 3 (P >0.05). Conclusion: The 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB as effectively as the 4% hydroquinone cream.Keywords: redfruit extract cream, melanin, UVBAbstrak: Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak buah merah 10% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB dan perbandingannya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Sampel terdiri dari tiga kelompok dengan jumlah sampel 10 ekor marmut jantan tiap kelompok. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan diolesi krim hidrokuinon 4%. Kelompok 3 diberi paparan sinar UVB dan diolesi krim ekstrak buah merah 10%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (19,78± 3,79%) dan terendah pada kelompok 2 (0,85±0,37%), sedangkan jumlah melanin pada kelompok 3 di antara keduanya (1,25±0,76%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Tidak terdapat perbedaan bermakna antara kelompok 2 dan 3 dalam mencegah peningkatan jumlah melanin (P >0,05 ). Simpulan: Krim ekstrak buah merah 10% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB sama efektif dengan krim hidrokuinon 4%.Kata kunci: krim ekstrak buah merah, melanin, UVB
Co-Authors ., Nuriyah ., Yulyani ., Zenitalia A. A. G. P. Wiraguna A.A. Gede Budhiarta A.A. Gede Budhiarta A.A. Gede Budhiarta A.A.G.P Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP. Wiraguna Agung Wiwiek Indrayani Agus Eka Darwinata Akpriyanti, DG Ika Aman, I G M Anak Agung Ayu Ngurah Susraini Anak Agung Gde Budhiarta Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Anak Agung Gede Budhiarta Anak Agung Gede Putra Wiraguna Andrew Lie Angelia Ongko Prabowo Anne Carolina Kencanawati Anshori, Ahmad M. Aprilany Stella peng Astried Haryanto Augus Venty Azizah, Norma Nur Azizah Bagus Komang Satriyasa Balqis Wasliati Balqis Wasliati Bissi Herliane Budhiarta, Anak A.G. Chandra, Aji B. Chynthia Friama Delly Dahlia Dessy Indriana Hatta Dewi, Dian Andriani Ratna Dian Andriani Dumaria, Corazon H. Dwindi Saptania Efrida Sumawati Erwin Sugito Eveline Margo Feily Sari Moelyono Fenni Liem Ferbian M. Siswanto, Ferbian M. Fiter, Joni Gde Ngurah Idraguna Pinatih Gina Septivani Hendrik Danico Heny Widiyowati S Hery Widijanto Huda, Siti M. N. I Gede Made Aman I Gede Widhiantara I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Ketut Suada I Ketut Tunas I Nyoman Adiputra I Nyoman Mangku Karmaya I NYOMAN MANTIK ASTAWA I Wayan Weta Ida Sri Iswari Indah Mira Tiaraputri Wijaya Ingrid Rotinsulu J. A. Pangkahila Junivan Lindra Kandy Rosa Ismalia Larasati, Veny Laura Indriana Liliana, Nize Lis Widodo Peni Liya Rosdiana Luh Putu Iin Indrayani Maker Made Ayu Mirah Wulandari Made Oka Negara MADE RATNA SARASWATI . Manus, Widya Christine Maulidha Suzi Annisa Maya Cesarya Simangunsong Meiny Linda Setiawan Meiyati Panambunan Melanie Maria Magdalena Ratulangi Mery Merysia Milhanah Milhanah Mochtar, Fransiska Muhammad Rahmatullah Ni Luh Putri Astini Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Sriwidyani Nina Marini Nining Triyana Darise Nurwiyanti, . Palumpun, Eva F. Periskila Abigail Phery Candres Putri Puspitasari R. A.T. Kuswardhani Rafiah Sirikit Saenong Riliani, Marisa Romy Kamaluddin Ronald Suryaprawira Rusmiatik Rusmiatik Sabella Setia Wardani Siahaan, Eva R. Siahaan, Marintan S. Y. Sofiana, Rahmi Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Tambor Tiur Marlina Tamon, Oktavian Tarnajaya, Karnia Thomas Eko Purwata Trisna Yuliharti Tersinanda Ursula Sulistiani Vany Novanty Vashti Saraswati Vitia Tandy Widhayanthi, Trisna A. Wiraguna AAGP Wiraguna, A A G P Wiraguna, A AGP Wiraguna, AAG Wiraguna, Anak A.G.P. Yuziana Yuziana