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Administration of oral tranexamic acid is equally effective with intradermal injections in reducing the amount of melanin in female marmots (Cavia porcellus) exposed to ultraviolet-b Ni Luh Putri Astini; Wimpie Pangkahila; AAGP Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Oral studies of tranexamic acid significantly reduce the MASI (Melasma Area and Severity Index) value but have not evaluated a decrease in the amount of melanin. The purpose of this study is to prove that oral tranexamic acid can reduce the amount of melanin and have the same effectiveness as intradermal injections in female guinea pigs exposed to ultraviolet B. Method: A posttest only control group design study was conducted using 32 female mice, aged 6-8 weeks, weight 250-280 grams, which were randomly divided into 2 groups. Group 1 was given an intradermal injection of tranexamic acid 5 mg/ml, 0.5 ml/cm and exposure to UVB. Group 2 was given oral treatment of tranexamic acid 250 mg twice a day and exposure to UVB. The treatment was given for 4 weeks. Total UVB exposure was 390mj/sec. The study was conducted in the animal laboratory of the Udayana medical faculty. Results: The statistic result showed that the data were normally distributed and homogenous. The comparative test with the independent sample t-test indicated that there was no significant difference between intradermal injections with oral tranexamic acid administration in reducing the amount of melanin in female guinea pigs exposed to UVB with p> 0.05. Conclusion: oral tranexamic acid significantly reduced the amount of melanin and had the same effectiveness as intradermal injections in reducing the amount of melanin in female guinea pigs exposed to UVB.
Topical administration of broccoli flower (brassica oleraceae l. Var italica plenck) extract cream 10% prevented increased of melanin in UVB exposed guinea pig (cavia porcellus) Tambor Tiur Marlina; Wimpie Pangkahila; AAGP Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.727 KB) | DOI: 10.36675/ijaam.v4i1.47

Abstract

Introduction: Skin aging is primarily caused by increased free radicals and decreased cellular anti-oxidant system in keratinocyte. Broccoli flower contains several important antioxidant molecules that can inhibit aging process of the skin. Therefore, the aim of this research is to prove the effectivity of administration broccoli flower extract cream on inhibiting the elevation of melanin level on guinea pig (Cavia porcellus) exposed with UV-B. Methods: An animal experiment with post-test only controls group design was conducted using 36 guinea pigs which were divided into 2 groups. The control group was treated with placebo cream while treatment group was treated with 10% broccoli flower extract cream. All groups were exposed with UV-B with dose at 390 mJ/cm2 for 2 weeks and biopsy was taken to examine the level of melanin. Results: The results showed that the average amount of melanin expression in the control group (P0) was 21.48 ± 1.48% while it was 1.32 ± 0.85% in treatment group (P1). Independent T-test analysis showed that the difference was statistically significant (t = 33.95; p = 0.001). Conclusion: It can be concluded that the topical administration of broccoli flower extract cream prevented increase in skin melanin in UV-B exposed guinea pig.
Excessive physical activity increased leucocyte and decreased Endorphin levels in male wistar rats (Rattus norvegicus) Periskila Abigail; Wimpie Pangkahila; Alex Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.61

Abstract

Background: Excessive physical activity triggers hormonal imbalances, one of which is beta endorphin and lead to increasing leukocytes as a response to the inflammation. The purpose of this study was to prove that excessive physical activity increased leucocyte and decreased endorphin levels in male Wistar rats (Rattus norvegicus). Methods: This study was an experimental study with randomized pretest-posttest group design. The research subjects were male rats (Rattus norvegicus), Wistar strain, aged 8-10 weeks with a body weight of 150-200 grams and healthy. A total of 14 rats were randomly divided into two groups. The first group was treated with balanced physical activity (P1), the second group was treated with excessive physical activity (P2). On day 8 and day 28, leukocytes were examined using whole blood method and beta endorphin were examined using ELISA. Results: The results showed that in the group P1, both leukocyte levels (from 14.0±2.73x103/µL to 17.1±5.07x103/µL, p= 0.170) and beta endorphin levels (from 176±30.2 to 161±11.1 ng/mL, p= 0.110) remained stable. Whereas, the P2 group experienced leukocyte reduction (from 12.3±2.19x103/µL to 23.7±2.92x103/µL, p <0.001) and decreased beta endorphin levels (from 212±46.0 ng/mL to 55.3±8.05 ng/mL, p <0.001). Conclusion: This study suggesting that excessive physical activity increased leucocyte and decreased endorphin levels in male Wistar rats (Rattus norvegicus).
Oral Ashitaba Aeaves (Angelica keiskei) Extract Prevented The Increase of Tyrosinase Levels and Total Melanin in The Ultraviolet B­Exposed Guinea Pig (Cavia porcellus) Skin Meiny Linda Setiawan; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.100

Abstract

Introduction: UVB rays stimulate the tyrosinase which activates the biosynthesis of melanin and cause skin aging characterized by hyperpigmentation. Ashitaba is a plant rich of chalcone, a flavonoid compound with tyrosinase inhibitor activity. This study aimed to prove that oral Ashitaba (Angelica keiskei) extract prevented the elevation of tyrosinase levels and total melanin in the UVB-exposed guinea pig (Cavia porcellus) skin. Methods: This study used randomized posttest only control group design. The subjects were guinea pigs (Cavia porcellus), male, healthy, local strains, aged 3-4 months, weighing 300-­350 grams, one hybrid, which were divided into 2 groups (n = 18). The first group was the control group (treatment with exposure to ultraviolet B and placebo), the second group was the treatment group (treatment with exposure to ultraviolet B and oral Ashitaba leaves extract of 25 mg/kgBB). After 2 weeks of treatment, tyrosinase level was examined by the ELISA method, while the amount of melanin was examined for Masson­Fontana staining. Results: The mean tyrosinase level in the control group after 2 weeks of treatment was higer (30.64 ± 4.19ng/ml) than on the treatment group (11.47 ± 0.62ng/ml) with (p<0.05). In addition, the average number of melanin in the control group was also higer (19.05 ± 2.53%) than the treatment group (1.85 ± 0.84%) (p <0.05). Conclusion: Based on the results of this study, it can be concluded that oral Ashitaba (Angelica keiskei) extract prevented the increase of tyrosinase levels and total melanin in the UVB-exposed guinea pig (Cavia porcellus) skin.
Cream of leaf pare extract (momordica caharantia l) 4% takes the improvement of effective melanin numbers with 4% hydrocuinon cream on marmut skin (cavia porcellus) heart which uses uvb ultraviolet light Mery Merysia; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Bitter melon leaves is one of the plants that can be used as an antioxidant and antimelanogenic. The active compounds in bitter melon leaves are flavonoids, phenolic acids, tannins and vitamin C. The purpose of this study was to determine the effectiveness of 4% pare (Momordica charantia) leaf extract cream with 4% hydroquinone cream in inhibiting the increase of melanin in marmot skin (Cavia porcellus) which was exposed to ultraviolet light B. Method: This study used experimental study with a randomized post-test only control group design method. This study used 36 male guinea pigs (Cavia porcellus), local strains, 3 months of age with a body weight of 300-350mg, divided into 2 groups, with 18 individuals each. The first group was received 4% hydroquinone cream and exposed to ultraviolet B while the second group received 4% bitter melon extract (Momordica charantia) and also exposed to ultraviolet light B 3x a week with a total irradiation dose of 390 mJ/cm2 for 2 weeks. Results: The results showed that the control group (P0) had a mean number of melanin 0.98 ± 0.25% pixel and the treatment group (P1) had an average number of melanin 1.17 ± 0.34% pixels. The difference was analyzed by t-independent test and found to be insignificant (p = 0.06). Statistical analysis found no significant differences in the number of melanin between the control group and the treatment group (p = 0.06). Conclusion: The results showed that the 4% ethanol extract of pare leaves (Momordica charantia) was as effective as hydroquinone cream in inhibiting the increase in the amount of melanin in the skin of male guinea pigs (Cavia porcellus) exposed to UVB rays.
Oral administration of melatonin increased superoxide dismutase and reduced malondialdehyde in male wistar rats (rattus norvegicus) induced by cigarette smoke Aprilany Stella peng; Wimpie Pangkahila; I Wayan Weta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.52

Abstract

Introduction: Cigarette smoke is one of the most common sources of free radicals that accelerate aging process. This process can be countered by avoiding cigarette and consuming anti-oxidants. Melatonin is one of well-known anti-oxidant which can be safely consumed. Therefore, the aim of this study was to assess the effect of oral melatonin supplementation toward the level of superoxide dismutase and malondialdehyde in male Wistar rats exposed by cigarette smoke. Methods: An experimental pretest-posttest control group study was conducted using 18 male Wistar rats, 12 weeks old, and 180-200 gr in weight. The subjects were divided into two groups (n= 9 rats) randomly. Both groups were exposed by cigarette smoke but treatment group (P1) was treated with 2 mg of melatonin per 200 gr BW rats for 14 days. The levels of SOD and MDA were measured using the ELISA method. Results: The baseline level of SOD and MDA in both groups was homogenous. At the end of the experiment, there was significant increase of SOD level (baseline: 1.13±0.45; post-treatment: 2.57±0.08 ng/mL; p= <0.001) and decreased in MDA level (baseline: 9.53 ± 1.25; post-treatment: 1.44 ± 0.46; p= <0.001) in treatment group while no change was observed in both SOD and MDA in control group. Conclusion: The oral consumption of melatonin countered the adverse effect of smoking by increasing the level of SOD and lowers MDA level.
Pemberian Krim Ekstrak Teh Hijau (Camellia sinensis) Dapat Mencegah Penurunan Jumlah Kolagen Dermis dan Peningkatan Kadar Matriks Metalloproteinase-1 pada Mencit Balb -C Yang Dipapar Sinar Ultraviolet B Heny Widiyowati S; Wimpie I Pangkahila; A.A.G.P. Wiraguna; J Alex Pangkahila; I Nyoman Adiputra; IGM. Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 1 No 1 (2017): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Ekstrak Teh Hijau mengandung polifenol utama dalam daun teh, yaitu katekin yang terdiri dari Epigallocathecin 3-gallate (EGCG), epigallocathecin (EGC), epicathecingallate (ECG) , epicatechin (EC), gallocathecin (GC). Diantara keempat komponen tersebut EGCG (Epigallocathecin Gallate) merupakan komponen yang paling potensial. Polifenol teh hijau memiliki efek peredaman terhadap ROS (Radical Oxidative Superoxide), sehingga dapat mencegah kerusakan kulit akibat dari sinar UV-B. Penelitian ini bertujuan untuk membuktikan pemberian krim ekstrak teh hijau 70% dapat mencegah penurunan jumlah kolagen dermis dan peningkatan ekspresi matriks metalloproteinase-1 pada mencit BALB-C. yang dipapar sinar ultraviolet-B.Penelitian ini adalah penelitian eksperimental laboratorium, menggunakan rancangan the randomized post-test only control group. Sebanyak 30 ekor mencit balb/c yang digunakan pada penelitian ini dibagi menjadi 3 kelompok yang masing-masing terdiri dari 10 ekor mencit. Kelompok Kontrol (O) tidak diolesi apapun, Kelompok 1 diolesi bahan dasar krim (Kelompok Perlakuan 1), dan Kelompok 2 diolesi krim ekstrak teh hijau 70% (Kelompok Perlakuan 2). Semua Kelompok Perlakuan diberikan paparan sinar UVB dengan dosis total 840 mJ/cm2 selama 4 minggu, kemudian dilakukan biopsi untuk permeriksaan jumlah kolagen dan ekspresi MMP-1. Data dianalisis menggunakan one way Anova untuk mengetahui adanya perbedaan signifikan pada ketiga kelompok perlakuan kemudian dilanjutkan uji post hoc dengan menggunakan tes LSD (Least Significance Difference) untuk mengetahui beda nyata terkecil pada taraf kemaknaan <0,05. Hasil penelitian menunjukkan terjadinya penurunan yang lebih rerata kolagen yang bermakna pada Kelompok Kontrol (47,83%) dan Kelompok Perlakuan 1 (50,73%) setelah diberikan paparan sinar UV-B dibandingkan dengan rerata kolagen pada Kelompok Perlakuan 2 (67,59%). Hasil rerata ekspresi MMP-1 terjadinya peningkatan pada rerata kontrol (66,76%) dan rerata Kelompok Perlakuan 1 (74,46%) dibandingkan rerata ekspresi MMP-1 Kelompok Perlakuan 2 (39,31%). Melalui uji post hoc tidak ada perbedaan hasil dari Kontrol dan Kelompok Perlakuan 1 baik pada jumlah kolagen dan ekspresi MMP-1 (P>0,05).Simpulan penelitian ini adalah krim ekstrak teh hijau 70% dapat mencegah penurunan jumlah kolagen dermis dan peningkatan ekspresi MMP-1 pada mencit Balb/C yang diberi paparan UV-B.
Administration of Creatine Monohydrate® increased the estrogen levels but have no effect on testosterone levels in male albino rats (rattus norvegicus) with moderate physical activity Ursula Sulistiani; Wimpie Pangkahila; Alex Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.79 KB) | DOI: 10.36675/ijaam.v3i2.46

Abstract

Background: Our laboratory assessment indicated that the Creatine Monohydrate® supplement contains phytoestrogen and phytoprogesteron. This study was aimed to prove that the administration of Creatine Monohydrate® supplement could increase the estrogen levels and decrease testosterone levels in male albino rats (Rattus norvegicus) with moderate physical activity given. Methods: A true experimental with pre-test and post-test randomized control study was conducted using 14 albino male rats. The samples were divided into two groups; the control groups (P0) that received aquadest and moderate physical activity, and treatment group (P1) that received 0.4 mg Creatine Monohydrate® supplement twice a day and moderate physical activity given. Moderate physical activity (swimming) was given five times a week for 14 days. Estrogen and testosterone level were assessed before and after treatment was given. Results: Before the treatment, the estrogen and testosterone levels between P0 group and P1 group were comparable. Post-test results showed a significant different in estrogen levels between the P0 and P1 group (23.17±2.86 and 27.82±3.06 ng/ml respectively; p<0.05) as well as testosterone levels (18.15±2.95 and 21.69±2.14 ng/ml respectively; p<0.05) after 14 days of treatment. A paired analysis showed an elevating of estrogen levels but no effect on testosterone levels in P1 group that treated with Creatine Monohydrate® Conclusion: This study indicated that the administration of Creatine Monohydrate® increased the estrogen levels but have no effect on testosterone levels in male albino rats (Rattus norvegicus) with moderate physical activity.
Administrations of young betelnut fruit extract (Areca cathecu l.) increased testosteron concentration in old male wistar white rat (Rattus norvegicus) Dessy Indriana Hatta; Wimpie Pangkahila; I Gede Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Background: With increasing age, testosterone level is tend to and cause sexual dysfunction disorders such as erectile dysfunction, sexual arousal disorders, ejaculatory disorders, orgasm disorders to infertility. Young betel nut extract (Areca catechu L.) is an herbal plant that can increase sexual arousal (libido) which has many nutrients including antioxidants. The purpose of this study was to prove that administration of young areca fruit extract could increase testosterone levels in old male wistar white rats (Rattus norvegicus). Method: This study was an experimental study using a pretest-posttest control group design that used 16 wistar male rats (Rattus norvegicus) aged 18 months old, weighing 225-250 grams divided into 2 (two) groups, namely the control group (Placebo ) and the treatment group who were given young betel nut extract 100 mg / kgBB / day (P1). The study was conducted for 28 days followed by blood sampling for testosterone levels. Results: The results showed significant increase in testosterone levels in treatment group compared to the control group, (p= <0.05)). The testosterone level increased from 14.813 ± 1.343 ng/ml before treatment to 19.602 ± 1.345 ng/ml after treatment. Conclusion: Based on the results of the analysis it can be concluded that the administration of Areca catechu L. extract can increase Testosterone levels in old male wistar white rats (Rattus norvegicus).
The application of black garlic (Allium sativum linn) extract cream in preventing the increase level of MMP-1 expression and the decrease of dermal collagen count in the skin of male wistar rats (Rattus norvegicus) exposed to UV-B Rays Balqis Wasliati; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.81

Abstract

Introduction: UV-B exposure triggers the activation of Protein-1 (AP-1) which disrupts the integrity of the extracellular matrix by inducing MMP-1. Black garlic causes an increase of antioxidant content namely phenolic, flavonoid (quercetin), and DPPH (IC-50). This study aimed to determine the application of black garlic (Allium sativum linn) extract cream in preventing the increased level of MMP-1 expression and the decrease of dermal collagen count in the skin of male Wistar rats exposed to UV-B rays. Methods: This study employed an experimental Post Test Only Control Group Design using 32 male Wistar rats. The rats that were applied with base cream material and exposed to UV-B rays were called the control group. The rats that were applied with 15% black garlic extract cream and exposed to UV-B rays were called the treatment group. The expression of MMP-1 was examined by immunohistochemistry and the dermal collagen count of the skin using Pico-Sirius-Red staining. Results: The mean expression of MMP-1 of the control group was 28.58% and the treatment group was 8.60% (p <0.001). The mean count of collagen in the control group was 57.53% and the treatment group was 83.80% (p <0.001). There was a significant difference in the MMP-1 expression and the count of collagen between the treatment of the control group and the treatment group. Conclusion: Administration of the cream of 15% black garlic extract was proven to prevent an increase MMP-1 expression and decrease dermal collagen count in the skin of male Wistar rats exposed to UV-B rays.
Co-Authors ., Nuriyah ., Yulyani ., Zenitalia A. A. G. P. Wiraguna A.A. Gede Budhiarta A.A. Gede Budhiarta A.A. Gede Budhiarta A.A.G.P Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP. Wiraguna Agung Wiwiek Indrayani Agus Eka Darwinata Akpriyanti, DG Ika Aman, I G M Anak Agung Ayu Ngurah Susraini Anak Agung Gde Budhiarta Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Anak Agung Gede Budhiarta Anak Agung Gede Putra Wiraguna Andrew Lie Angelia Ongko Prabowo Anne Carolina Kencanawati Anshori, Ahmad M. Aprilany Stella peng Astried Haryanto Augus Venty Azizah, Norma Nur Azizah Bagus Komang Satriyasa Balqis Wasliati Balqis Wasliati Bissi Herliane Budhiarta, Anak A.G. Chandra, Aji B. Chynthia Friama Delly Dahlia Dessy Indriana Hatta Dewi, Dian Andriani Ratna Dian Andriani Dumaria, Corazon H. Dwindi Saptania Efrida Sumawati Erwin Sugito Eveline Margo Feily Sari Moelyono Fenni Liem Ferbian M. Siswanto, Ferbian M. Fiter, Joni Gde Ngurah Idraguna Pinatih Gina Septivani Hendrik Danico Heny Widiyowati S Hery Widijanto Huda, Siti M. N. I Gede Made Aman I Gede Widhiantara I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Ketut Suada I Ketut Tunas I Nyoman Adiputra I Nyoman Mangku Karmaya I NYOMAN MANTIK ASTAWA I Wayan Weta Ida Sri Iswari Indah Mira Tiaraputri Wijaya Ingrid Rotinsulu J. A. Pangkahila Junivan Lindra Kandy Rosa Ismalia Larasati, Veny Laura Indriana Liliana, Nize Lis Widodo Peni Liya Rosdiana Luh Putu Iin Indrayani Maker Made Ayu Mirah Wulandari Made Oka Negara MADE RATNA SARASWATI . Manus, Widya Christine Maulidha Suzi Annisa Maya Cesarya Simangunsong Meiny Linda Setiawan Meiyati Panambunan Melanie Maria Magdalena Ratulangi Mery Merysia Milhanah Milhanah Mochtar, Fransiska Muhammad Rahmatullah Ni Luh Putri Astini Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Sriwidyani Nina Marini Nining Triyana Darise Nurwiyanti, . Palumpun, Eva F. Periskila Abigail Phery Candres Putri Puspitasari R. A.T. Kuswardhani Rafiah Sirikit Saenong Riliani, Marisa Romy Kamaluddin Ronald Suryaprawira Rusmiatik Rusmiatik Sabella Setia Wardani Siahaan, Eva R. Siahaan, Marintan S. Y. Sofiana, Rahmi Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Tambor Tiur Marlina Tamon, Oktavian Tarnajaya, Karnia Thomas Eko Purwata Trisna Yuliharti Tersinanda Ursula Sulistiani Vany Novanty Vashti Saraswati Vitia Tandy Widhayanthi, Trisna A. Wiraguna AAGP Wiraguna, A A G P Wiraguna, A AGP Wiraguna, AAG Wiraguna, Anak A.G.P. Yuziana Yuziana