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Topical bromelain cream did not increase neovascularization, fibroblast and epithelialization in wound healing of diabetic-rats (rattus norvegicus) Maulidha Suzi Annisa; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.90

Abstract

Introduction: The diabetic-wound healing process is longer due to blood vessel ischemia. Bromelain is the enzyme that acts as debridement agent with anti-inflammatory effect. The aim of this study was to prove the topical administration of bromelain increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats. Methods: This study used post-test only control group design. Subjects were 32 male Wistar diabetic-rats (Rattus norvegicus. It was divided into 2 groups (n = 16) and given amoxiciline for 3 days. The control group consist of: O1 group treated with a base cream for 5 days and O2 group was treated for 12 days. While the treatment group consist of: O3 group was treated with 70% bromelain cream for 5 days and O4 group was treated for 12 days. The neovascularization, fibroblasts, and epithelialization were examined histopathologically. Results: The mean number of fibroblasts in O1 and O3 were 222.88±37.94 cells/field of view and 25.38±7.94 cells/field of view, while O2 and O4 were 99.63±82.33 cells/field of view and 32.25±12.453 cells/field of view (p <0.001). The mean number of neovascularization in O1 and O3 were 15.13±1.95 and 2.88±1.80 cells/field of view, while O2 and O4 were 5.50±3.78 cells/field of view and 1.50±1.06 cells/field of view (p <0.001). The epithelial gap size in the O1 and O2 group were 377.56±573.88 μm and 229.04±647.83 μm (p <0,001). Both O3 and O4 groups showed no longer a wound gap (p>0,05). Conclusion: Topical bromelain cream 70% did not increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats.
Administration of Moringa (Moringa oleifera) Fruit Seed Extract Cream 6% Prevented Collagen Depletion in Ultraviolet B-Induced Guinea Pigs (Cavia Porcelus) Skin Yuziana Yuziana; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.74

Abstract

ABSTRACT Background: Skin is often exposed to the environment such as Ultraviolet (UV) radiation which causes skin aging or photoaging. UVB-induced photoaging is characterized by a decrease in the amount of collagen. Natural ingredients that contain antioxidants, such as Moringa fruit seed extract containing bioactive compounds, are expected to prevent a decrease in the amount of collagen that eventually slows skin aging. The purpose of this study was to prove that administration of Moringa (Moringa oleifera) fruit seed extract cream 6% prevents collagen depletion in ultraviolet B-induced guinea pigs (Cavia porcelus) skin. Methods: This study was an experimental study with post-test only control group design. The subjects used in this study were 36 guinea pig (Cavia porcelus), 3 months old, male, and weighing 300-350 gr. The guinea pigs were grouped randomly into 2 groups with 18 guinea pigs/group. The first group (control) was the group treated with basic cream and exposed to UVB rays of 840 mJ/cm2; while the second group (treatment group) was treated with Moringa fruit seed extract cream 6% and exposed to UVB rays of 840 mJ/cm2. After 28 days of treatment, the guinea pigs were euthanized and histopathological examination of collagen was carried out with Sirius red staining. Results: The results showed that the average amount of collagen in the control group was 59.73 ± 2.31%. In the group of Moringa fruit seed extract cream 6%, the collagen amount was 84.07 ± 2.03%. Significance analysis using independent t-test showed a p value of <0.001 which means that there was a difference in the average amount of collagen between groups. Conclusion: In this study, the results indicated that administration of Moringa (Moringa oleifera) fruit seed extract cream 6% prevented collagen depletion in ultraviolet B-induced guinea pigs (Cavia porcelus) skin. Keywords: Moringa fruit seeds, collagen, guinea pigs, ultraviolet B.
Administration of gallus domesticus boiled chicken white egg increased amh levels of premenopausal female wistar rats Liya Rosdiana; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Aging is a complex multifactorial process and leads to functional changes one of which is the decline in reproductive function. Decrease in reproductive function in women in in the form of decreased ovarian reserve can be detected by low levels of Anti_mullerian Hormone (AMH). This study aims to prove the effect of administration of Gallus domesticus boiled chicken white egg increased AMH levels of premenopausal female wistar rats. Methods: This research was experimental animal with Pretest-Posttest Control Group Design. The study conducted on 16 premenopausal female wistar mices, aged 17-18 months, weighing 250-260 grams. The mice were divided into 2 goups: the control group (placebo), which was given gelatin flour by 7.3 gram 3 times daily for 4 weeks and the group treted with Gallus domesticus boiled chicken white egg by 7.3 gram 3 times daily for 4 weeks by oral. Blood AMH level were measured before and after treatment for both group. Results: The result showed that before treatment, mean AMH level both in control group and treatment group were not significantly different (p > 0,05). After treatment, mean AMH level both in control group and treatment group were significantly different (p < 0,05). The administration of Gallus domesticus boiled chicken white egg increased AMH levels by 0.804 ng/ml. Conclusion: It can be concluded that administration of Gallus domesticus boiled chicken white egg increased AMH level. The mean AMH level after treatment had a significant difference between control group (placebo) and treatment group with p = 0.001.
Topical application of cinnamon (cinnamomum burmanii) essential oil has the same effectiveness as minoxidil in increasing hair length and diameter size of hair follicles in male white Wistar rats (rattus norvegicus) Laura Indriana; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: In aging process, the hair experiences thinning and the hair shaft becomes more fragile due to decreased regeneration of hair. Cinnamon essential oil contains cinnamaldehyde compounds which have a vasodilating effect on blood vessels and stimulating IGF-1 can increase fertility and prevent hair loss. Methods: This study was an experimental study with a post test only control group design carried out in the laboratory animal unit of the pharmacology section of the Udayana University medical faculty. In this study 30 male white rats (Rattus norvegicus) wistar lines were sheared 4cmx4cm which were divided into three groups: negative (aquades) control group (2% minoxidil), treatment group with cinnamon essential oil (concentration 100 %) for 30 days. Results: The average hair length of aquades (P0) was 5.88 ± 0.36 mm, minoxidil group 2% (P1) 7.4 ± 0.20 mm. , cinnamon essential oil group (P2) 8.32 ± 0.18 mm In the diameter size of hair follicles in the three groups showed the mean diameter size of hair follicles was significantly different (P <0.05). The average results of the group hair follicle diameter (P0) were 79.73 ± 30.68 µm, group (P1) 127.07 ± 38.20 µm and group (P2) 130.91 ± 49.96 µm. The LSD test results on hair length measurements between the three groups showed a very significant difference (P <0.01). Group P0 and P1-1,51 mm, group P0 and P2-2,44 mm group P1 and P2 0,92 mm. The results of LSD hair follicle diameter P0 and P1 -47.34 µm (P <0.05), P0 and P2 -51.18 µm (P <0.01), P1 and P2 -3.84 µm (P> 0 , 05) Conclusion: The application of topical cinnamon essential oil increases hair length and diameter size of male wistar rat hair follicles as effectively as topical minoxidil 2%.
Ashitaba (Angelica keiskei) leaves extract prevents spatial memory impairment in male balb/c mice (Mus musculus) with brain aging Indah Mira Tiaraputri Wijaya; Wimpie Pangkahila; Thomas Eko Purwata
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.64

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Background: Memory impairment is one of the symptoms of aging process which can be caused by free radicals or oxidative stress. Antioxidants such as flavonoids are compounds that can inhibit reactive oxygen species (ROS). Ashitaba (Angelica keiskei) is one of the natural compound that contains high flavonoids. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract prevents spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging Methods: Experimental randomized pretest-posttest group using 26 mice, aged 3 months, healthy (active and willing to eat), weighing 20-30 grams. Mice were induced with oral D-Galactose of 300 mg/kgBW/day for 4 consecutive weeks to achieve the brain aging condition. Control group treated with placebo (2 ml of aquabidest), and another group was treated with Ashitaba leaves extract of 20 mg/kgBW/day. Before and after treatment for 28 days, spatial memory were examined using the Morris Water Maze method. Results: The results showed a significant decrease in probe test (from 72,9±5,69% to 40,8±5,10%; p<0,001) and the number of platform crossings (from 7,37±0,527 times to 4,50 ± 0,771 times; p<0,001) in the control group suggesting that D-Galactose-only treatment impaired spatial memory. In contrast, the group treated with D-Galactose and Ashitaba leaves extract, there was no change in probe test (from 72,1±5,9% to 66,0±6,72%; p>0,05) and number of platform crossings (from 7,65±0,881 times to 6,98±0,787 times; p>0,05). Conclusion: This study indicated that Ashitaba (Angelica keiskei) leaves extracts prevented spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging.
Combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract increased pancreatic beta cells number but have no effect toward glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus) Meiyati Panambunan; Wimpie Pangkahila; Anak Agung Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.908 KB) | DOI: 10.36675/ijaam.v3i2.43

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Introduction: Diabetes mellitus is a complex and progressive disease which often lead to several debilitating complications that partly caused by by free radicals which can be overcome with antioxidants. Bay leaves contain essential oils, tannins, flavonoids and terpenoids which have considerable antioxidant properties. Therefore, this study aims to determine the effect combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract toward pancreatic beta cells density and glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus). Methods: An experimental posttest only control group study was conducted using 36 albino male rats, aged 2-3 months, weighing 180-200 grams. The rats were divided into 2 groups (n= 18) with control group treated with 1.8mg/200g body weight vildagliptin and 2cc placebo (aquabidest) while the treatment group received 1.8mg/200g BW vildagliptin and 250 mg/200g BW bay leaves extract. All treatment lasted for 21 days. Results: The results showed that pancreatic beta cell counts in the treatment group was significantly higher than the control group (109.07 ± 20.47 cells/field of view vs 90.87 ± 13.91 cells/field of view; p<0.01). However, the levels of Glycated albumin between two groups were not significantly different (treatment vs control: 17.33 ± 4.51 vs 20.18 ± 4.57; p=0.068). Conclusion: This study suggested that combination treatment of vildagliptin and bay leaf extract increased pancreatic beta cells but did not reduce glycated albumin levels in diabetic male Wistar rats.
Oral administration of clabet (Trigonella foenum graecum) seeds ethanol extracts improved testosteron level in old-male wistar (Rattus norvegicus) mice Bissi Herliane; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.464 KB)

Abstract

Introduction: Aging can cause decreased testosterone levels in men which is known as andropause. Some herbs are known to increase the levels Testosterone. One of them is Klabet seed that contain phyto-testosterone. The purpose of this study was to prove that the administration of ethanol extract of Klabet seeds increased testosterone levels in old male Wistar rats. Methods: A pretest-posttest control group design study was conducted using 20 male white wistar rats that were divided into 2 (two) groups: control group that received 2 cc of distilled water and treatment group that received 2 cc ethanol extract of Klabet seeds 250mg/250gBB rats/day by sonde for 14 days. The testosterone level was measured on day 1 and 15. Results: The analysis showed that there was no significant difference in the pre-test and post-test testosterone level P0 (Pre-test:16.4±0.553 ng/ml; posttest: 15.9±1.51ng/ml) while increased level of testosterone could be observed in treatment group (P1) (pre-test: 16.6±0.476ng/ml; post-test: 19.4±0.828ng/ml. Comparative analysis between post-test results (P0 vs P1) showed that the difference was statistically significant (p <0.001). Conclusion: Oral administration of Klabet seeds ethanol extract can significantly increase testosterone level in old male wistar mice.
Administration of 5% Tranexamic Acid Cream Reduce the Amount of Melanin as Effective as 4% Hydroquinone Cream in Female Guinea Pigs (Cavia porcellus) Exposed to Ultraviolet-B Light Nina Marini; Wimpie Pangkahila; AAGP. Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.31

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Introduction: Exposure to ultraviolet B light will increase the amount of melanin which is a common sign of skin aging, one of which is hyperpigmentation which causes psychological impacts, causing social disturbances. Currently, no effective treatment has been found, where hydroquinone as the gold standard provides dangerous side effects, so a more effective and safe material is needed for long-term use. The aim of this study was to prove that tranexamic acid cream was as effective as hydroquinone in reducing the amount of melanin in female guinea pigs (Cavia porcellus) exposed to UVB rays. Methods: This study used a post test only control group design with 36 adult female guinea pigs exposed to UVB rays for 6 weeks at 60mJ/cm2 three times a week. After 2 weeks the guinea pigs were randomly divided into 2 groups with 18 guinea pigs in each group. Group A was given 4% hydroquinone cream and group B was given tranexamic acid cream 5%. Exposure to UVB rays was continued as long as the guinea pig was given the cream treatment. After 42 days the guinea pigs were biopsied to determine the amount of epidermal melanin. Results: The results showed that the mean amount of melanin in the hydroquinone group was 7.40 ± 3.61 and in the tranexamic acid group was 7.27 ± 4.06 (p=0.925; p>0.05). There was no significant difference between the hydroquinone cream group and the tranexamic sour cream group. Conclusion: This study concluded that administration of 5% tranexamic acid cream reduced the amount of melanin as effective as 4% hydroquinone cream in female guinea pigs exposed to UVB light.
Administration of Oral Sorghum Seed Extract (Sorghum bicolor L.) Increased Pancreatic Beta Cells Count but did not Reduce Glycated Albumin Levels in Male Wistar Rats (Rattus Norvegicus) with Diabetes Mellitus Efrida Sumawati; Wimpie Pangkahila; A.A. Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.91

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Background: It is required a long time of diabetes mellitus treatment, such as doing general activities or even insulin. Sorghum is a plant that contains antioxidants. The aim of this research was to prove the oral administration of Sorghum Seed Extract (Sorghum bicolor L.) in increasing the pancreatic beta cells count but did not reduce the levels of glycated albumin in male Wistar rats (Rattus norvegicus) with Diabetes Mellitus. Method: This study used experimental post-test only group design. 36 male Wistar rats were used and injected with the nicotinamide and streptozotocin intraperitoneally. Rats were divided into 2 groups, the control group (n=18) was administrated with distilled water at a dose of 2cc/200g body weight of rats. The the treatment group (n=18) was administrated with ethanol extract of sorghum seeds with a dose of 80 mg/kgBW of rats/ day. The post-test of glycated albumin levels and histopathology examination of pancreatic beta cells count were performed. Results: The mean of pancreatic β cells count in the control group was 64.31 ± 19.397. It was lower than the treatment group which was 119.44 ± 33.799 (p <0.005). The mean results glycated albumin level of the research in the control group was 8.577 ± 3.105 ng/ml and in the treatment group was 9,191 ± 3,014 ng/ml (p> 0,05), therefore it was not proven. Conclusion: The administration of ethanol extract of sorghum seeds increased pancreatic β cells count, but it did not reduce glycated albumin levels in male Wistar rats (Rattus Norvegicus) with Diabetes Mellitus.
Nutrilite green trim® reduced body weight, waist circumference, and body fat percentage in obese men with moderate physical exercise and low-calorie diet Romy Kamaluddin; Wimpie Pangkahila; Alex Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.75

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Introduction: Management of obesity are including consumption of supplements. Nutrilite GreenTrim® Supplement contains green tea extract and EGCG and squalene. This study aimed to prove the effects of Nutrilite GreenTrim® on body weight, waist circumference, and body fats percentage in obese men. Methods: This clinical trial used pretest-posttest control group design in 22 obese men, aged 25-40 years with a BMI 25-35 kg/m2. Subjects were divided into the control group (K) and the treatment group (P). Subjects were given moderate physical exercise and a low-calorie diet. Results: The results showed K group lost weight (from 85.1±8.99 to 82.3±9.21 kg; p<0.001), while the P group lost weight (from 84.1±10.7 to 80.1±10.9 kg; p <0.001) and the difference showed that the P group lost weight greater than the K group (p = 0.007). K group experienced a decrease in waist circumference (from 96.9±7.69 to 93.8±8.84 cm; p <0.001), while the P group (from 96.5±7.78 to 92.2±7.59 cm; p <0.001) the difference showed that the decrease in waist circumference in the P group was better than the K group (p = 0.041). In the K group, body fat percentage decreased (from 27.5±3.31 to 25.8±3.77%; p <0.001), the P group also decreased (from 26.5±3.78 to 23.3±3.67%; p<0.001) the analysis showed that the decrease in body fat percentage in the P group was better than the K group (p <0.001). Conclusion: Supplementation of Nutrilite Green Trim® reduced body weight, waist circumference, and body fats percentage in obese men with moderate physical exercise and low-calorie diet.
Co-Authors ., Nuriyah ., Yulyani ., Zenitalia A. A. G. P. Wiraguna A.A. Gede Budhiarta A.A. Gede Budhiarta A.A. Gede Budhiarta A.A.G.P Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP. Wiraguna Agung Wiwiek Indrayani Agus Eka Darwinata Akpriyanti, DG Ika Aman, I G M Anak Agung Ayu Ngurah Susraini Anak Agung Gde Budhiarta Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Anak Agung Gede Budhiarta Anak Agung Gede Putra Wiraguna Andrew Lie Angelia Ongko Prabowo Anne Carolina Kencanawati Anshori, Ahmad M. Aprilany Stella peng Astried Haryanto Augus Venty Azizah, Norma Nur Azizah Bagus Komang Satriyasa Balqis Wasliati Balqis Wasliati Bissi Herliane Budhiarta, Anak A.G. Chandra, Aji B. Chynthia Friama Delly Dahlia Dessy Indriana Hatta Dewi, Dian Andriani Ratna Dian Andriani Dumaria, Corazon H. Dwindi Saptania Efrida Sumawati Erwin Sugito Eveline Margo Feily Sari Moelyono Fenni Liem Ferbian M. Siswanto, Ferbian M. Fiter, Joni Gde Ngurah Idraguna Pinatih Gina Septivani Hendrik Danico Heny Widiyowati S Hery Widijanto Huda, Siti M. N. I Gede Made Aman I Gede Widhiantara I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Ketut Suada I Ketut Tunas I Nyoman Adiputra I Nyoman Mangku Karmaya I NYOMAN MANTIK ASTAWA I Wayan Weta Ida Sri Iswari Indah Mira Tiaraputri Wijaya Ingrid Rotinsulu J. A. Pangkahila Junivan Lindra Kandy Rosa Ismalia Laura Indriana Liliana, Nize Lis Widodo Peni Liya Rosdiana Luh Putu Iin Indrayani Maker Made Ayu Mirah Wulandari Made Oka Negara MADE RATNA SARASWATI . Manus, Widya Christine Maulidha Suzi Annisa Maya Cesarya Simangunsong Meiny Linda Setiawan Meiyati Panambunan Melanie Maria Magdalena Ratulangi Mery Merysia Milhanah Milhanah Mochtar, Fransiska Muhammad Rahmatullah Ni Luh Putri Astini Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Sriwidyani Nina Marini Nining Triyana Darise Nurwiyanti, . Palumpun, Eva F. Periskila Abigail Phery Candres Putri Puspitasari R. A.T. Kuswardhani Rafiah Sirikit Saenong Riliani, Marisa Romy Kamaluddin Ronald Suryaprawira Rusmiatik, Rusmiatik Sabella Setia Wardani Siahaan, Eva R. Siahaan, Marintan S. Y. Sofiana, Rahmi Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Tambor Tiur Marlina Tamon, Oktavian Tarnajaya, Karnia Thomas Eko Purwata Trisna Yuliharti Tersinanda Ursula Sulistiani Vany Novanty Vashti Saraswati Veny Larasati Vitia Tandy Widhayanthi, Trisna A. Wiraguna AAGP Wiraguna, A A G P Wiraguna, A AGP Wiraguna, AAG Wiraguna, Anak A.G.P. Yuziana Yuziana