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Oral administration of bitter gourd (Momordica Charantia) extract prevent dyslipidemia on male wistar rats (rattus norvegicus) fed with high-fat diet Hendrik Danico; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.49

Abstract

Introduction: Dyslipidemia is one of the most prevalent health conditions and often leads to development of many chronic diseases such as coronary artery disease, heart failure, and stroke. This study was conducted to investigate the effects of bitter gourd (Momordica Charantia) extract on lipid profiles in male wistar rats fed with high-fat diet. Methods: This study was an experimental research using randomized post-test only control group design. The subjects were 36 male Wistar rats (Rattus norvegicus) randomly divided into 2 groups. The first group was the control group (P0) and fed with placebo while the treatment group (P1) received bitter gourd (Momordica Charantia) ethanol extract at 1g/kg body weight/day. Both groups were fed with high fat diet. The experiment was lasted for 35 days and lipid profiles were measured at the end of the experiment. Results: Statistical analysis results revealed the mean of total cholesterol, triglyceride, and low-density lipoprotein (LDL) in the treatment group were significantly lower than control group while high-density lipoprotein (HDL) was significantly higher in the treatment group (p<0.05). Conclusion: This study concluded that bitter gourd extract significantly prevent dyslipidemia on male Wistar rats fed with high-fat diet.
Administration of Ashitaba (Angelica Keiskei) leaves extracts prevented Superoxide Dismutase (SOD) Reduction and Malondialdehyde (MDA) elevation in overtrained-male wistar rats (Rattus norvegicus) Ronald Suryaprawira; Alex Pangkahila; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.63

Abstract

Introduction: The aging process is caused by several factors, one of which is the accumulation of oxidative damage caused by high free radicals in the body. Ashitaba (Angelica keiskei) leaves extract contains flavonoids, polyphenols, tannins, and antioxidant capacity. This study aimed to prove that administration of Ashitaba leaves extracts prevent superoxide dismutase (SOD) reduction and malondialdehyde (MDA) elevation in overtraining male Wistar rats (Rattus norvegicus). Methods: This study was an experimental study with randomized pretest-posttest control group design. The subjects used in this study were 16 male Wistar rats, healthy, aged 6 months, and weighing 180- 200 grams. The control group was given overtraining two hours after aquabidest 2 cc treatment (P0), and the treatment group was given overtraining two hours after administration of Ashitaba leaves extract of 20 mg/kg BW/day (P1). Before and after treatment for 14 days, blood sample were drawn through canthus medial orbital sinus to measure the level of SOD and MDA using the ELISA method. Results: The results showed in control group there was a decrease in SOD levels (from 8.58±0.78 ng/mL to 5.93±1.27 ng/mL; p<0.001) and increased MDA levels (from 2.75±0.19 nmol/mL to 3.28±0.23 nmol/mL; p= 0.001). In treatment group there was no change in SOD levels (from 8.90±0.61 ng/mL to 8.57±0.49 ng/mL; p= 0.217) and there was no change in MDA levels (from 2.67±0.33 nmol/mL to 2.62±0.24 nmol/mL; p= 0.693). Conclusion: It can be concluded that administration of Ashitaba leaves extracts prevented SOD reduction and MDA elevation in overtraining male Wistar rats.
DHEA supplementation increases epithelial thickness, plain muscle tissue, and adult vaginal blood vessels (rattus norvegicus) after bilateral post-ovarectomy Lis Widodo Peni; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Seventy-five percent of menopausal women have menopausal syndrome, one of which is vaginal atrophy. DHEA is the only source of postmenopausal sex steroid hormones that provides estrogen and androgens according to cell needs to function normally. The aim of this study is to prove that DHEA supplementation orally can increase epithelium thickness, smooth muscle tissue, and the number of vaginal blood vessels of adult white mice (Rattus norvegicus) after bilateral ovarectomy. Method: This study used true experimental post-test only control group design using 36 white mice aged 10-12 weeks with a body weight of 180-200 grams after bilateral ovarectomy. The mice were divided into two groups with 18 mice each. The first group (controls) was given glycerin orally at a dose of 1 ml per day while the second group (treatment) was given 7.2 mg DHEA dissolved in 1 ml glycerin orally per day. On day 43, vaginal tissue was taken and histopathological examination was performed. Result: The results showed that the mean of epithelial thickness of treatment group was significantly higher than control group (51.397 µm vs. 29.755 µm; p: < 0.05). The smooth muscle thickness was also significantly higher in treatment group compared to control (74.136 µm vs. 47.428 µm; p: <0.05). Finally, the number of blood vessels was also significantly higher in treatment group (18.39 vs. 9.50; p:<0.05). Conclusion: It can be concluded that DHEA supplementation increases epithelium thickness, smooth muscle tissue thickness, and the number of vaginal blood vessels in adult rats after bilateral ovarectomy.
Administration of Ashitaba (Angelica keiskei) Leaf Extracts Cream Prevented The Increase of MMP-1 Expression and The Decrease of Collagen Number in The Male Wistar Rats (Rattus norvegicus) Skin Exposed to UVB Phery Candres; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.102

Abstract

Introduction: Ultraviolet, especially the ultraviolet B (UV-B), is the most dominant cause of skin aging through increasing collagen degradation and activation of matrix metalloproteinase (MMPs). Recently, a lot of research focus on finding natural antioxidants with photoprotective activity. Ashitaba (Angelica keiskei) contains antioxidants and chalcone flavonoids. The purpose of this study was to examine the effect of ashitaba leaf extract, whether it could prevent increasing MMP-1 expression and decreasing collagen amount in rats exposed to UV-B rays. Methods: This study used randomized posttest only control group design. Subjects were 36 albino rats (Rattus norvegicus), male, adult (2.5 - 3 months old), weighing between 180-200 grams. All rats were divided into 18 rats each, namely the control group (the subject was smeared with basic cream and exposed to UVB) and the treatment group (the subject was smeared with ashitaba extract cream and exposed to UVB). The expression of MMP-1 using immunohistochemical staining and the amount of collagen using Sirius red staining. Results: The mean expression of MMP-1 in the control group was 28.14 ± 2.89% and in the treatment group was statistically lower at 9.45 ± 2.67% (p <0.001). The average amount of collagen in the control group was 53.64 ± 2.58% and in the treatment group was statistically higher at 83.57 ± 2.47% (p <0.001). Conclusion: The administration of ashitaba (Angelica keiskei) leaf extracts cream prevented the increase of MMP-1 expression and the decrease of collagen number in the male wistar rats (Rattus norvegicus) skin exposed to ultraviolet B.
Topical bromelain cream did not increase neovascularization, fibroblast and epithelialization in wound healing of diabetic-rats (rattus norvegicus) Maulidha Suzi Annisa; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.90

Abstract

Introduction: The diabetic-wound healing process is longer due to blood vessel ischemia. Bromelain is the enzyme that acts as debridement agent with anti-inflammatory effect. The aim of this study was to prove the topical administration of bromelain increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats. Methods: This study used post-test only control group design. Subjects were 32 male Wistar diabetic-rats (Rattus norvegicus. It was divided into 2 groups (n = 16) and given amoxiciline for 3 days. The control group consist of: O1 group treated with a base cream for 5 days and O2 group was treated for 12 days. While the treatment group consist of: O3 group was treated with 70% bromelain cream for 5 days and O4 group was treated for 12 days. The neovascularization, fibroblasts, and epithelialization were examined histopathologically. Results: The mean number of fibroblasts in O1 and O3 were 222.88±37.94 cells/field of view and 25.38±7.94 cells/field of view, while O2 and O4 were 99.63±82.33 cells/field of view and 32.25±12.453 cells/field of view (p <0.001). The mean number of neovascularization in O1 and O3 were 15.13±1.95 and 2.88±1.80 cells/field of view, while O2 and O4 were 5.50±3.78 cells/field of view and 1.50±1.06 cells/field of view (p <0.001). The epithelial gap size in the O1 and O2 group were 377.56±573.88 μm and 229.04±647.83 μm (p <0,001). Both O3 and O4 groups showed no longer a wound gap (p>0,05). Conclusion: Topical bromelain cream 70% did not increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats.
Administration of Moringa (Moringa oleifera) Fruit Seed Extract Cream 6% Prevented Collagen Depletion in Ultraviolet B-Induced Guinea Pigs (Cavia Porcelus) Skin Yuziana Yuziana; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.74

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ABSTRACT Background: Skin is often exposed to the environment such as Ultraviolet (UV) radiation which causes skin aging or photoaging. UVB-induced photoaging is characterized by a decrease in the amount of collagen. Natural ingredients that contain antioxidants, such as Moringa fruit seed extract containing bioactive compounds, are expected to prevent a decrease in the amount of collagen that eventually slows skin aging. The purpose of this study was to prove that administration of Moringa (Moringa oleifera) fruit seed extract cream 6% prevents collagen depletion in ultraviolet B-induced guinea pigs (Cavia porcelus) skin. Methods: This study was an experimental study with post-test only control group design. The subjects used in this study were 36 guinea pig (Cavia porcelus), 3 months old, male, and weighing 300-350 gr. The guinea pigs were grouped randomly into 2 groups with 18 guinea pigs/group. The first group (control) was the group treated with basic cream and exposed to UVB rays of 840 mJ/cm2; while the second group (treatment group) was treated with Moringa fruit seed extract cream 6% and exposed to UVB rays of 840 mJ/cm2. After 28 days of treatment, the guinea pigs were euthanized and histopathological examination of collagen was carried out with Sirius red staining. Results: The results showed that the average amount of collagen in the control group was 59.73 ± 2.31%. In the group of Moringa fruit seed extract cream 6%, the collagen amount was 84.07 ± 2.03%. Significance analysis using independent t-test showed a p value of <0.001 which means that there was a difference in the average amount of collagen between groups. Conclusion: In this study, the results indicated that administration of Moringa (Moringa oleifera) fruit seed extract cream 6% prevented collagen depletion in ultraviolet B-induced guinea pigs (Cavia porcelus) skin. Keywords: Moringa fruit seeds, collagen, guinea pigs, ultraviolet B.
Administration of gallus domesticus boiled chicken white egg increased amh levels of premenopausal female wistar rats Liya Rosdiana; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.108 KB)

Abstract

Introduction: Aging is a complex multifactorial process and leads to functional changes one of which is the decline in reproductive function. Decrease in reproductive function in women in in the form of decreased ovarian reserve can be detected by low levels of Anti_mullerian Hormone (AMH). This study aims to prove the effect of administration of Gallus domesticus boiled chicken white egg increased AMH levels of premenopausal female wistar rats. Methods: This research was experimental animal with Pretest-Posttest Control Group Design. The study conducted on 16 premenopausal female wistar mices, aged 17-18 months, weighing 250-260 grams. The mice were divided into 2 goups: the control group (placebo), which was given gelatin flour by 7.3 gram 3 times daily for 4 weeks and the group treted with Gallus domesticus boiled chicken white egg by 7.3 gram 3 times daily for 4 weeks by oral. Blood AMH level were measured before and after treatment for both group. Results: The result showed that before treatment, mean AMH level both in control group and treatment group were not significantly different (p > 0,05). After treatment, mean AMH level both in control group and treatment group were significantly different (p < 0,05). The administration of Gallus domesticus boiled chicken white egg increased AMH levels by 0.804 ng/ml. Conclusion: It can be concluded that administration of Gallus domesticus boiled chicken white egg increased AMH level. The mean AMH level after treatment had a significant difference between control group (placebo) and treatment group with p = 0.001.
Topical application of cinnamon (cinnamomum burmanii) essential oil has the same effectiveness as minoxidil in increasing hair length and diameter size of hair follicles in male white Wistar rats (rattus norvegicus) Laura Indriana; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.501 KB)

Abstract

Introduction: In aging process, the hair experiences thinning and the hair shaft becomes more fragile due to decreased regeneration of hair. Cinnamon essential oil contains cinnamaldehyde compounds which have a vasodilating effect on blood vessels and stimulating IGF-1 can increase fertility and prevent hair loss. Methods: This study was an experimental study with a post test only control group design carried out in the laboratory animal unit of the pharmacology section of the Udayana University medical faculty. In this study 30 male white rats (Rattus norvegicus) wistar lines were sheared 4cmx4cm which were divided into three groups: negative (aquades) control group (2% minoxidil), treatment group with cinnamon essential oil (concentration 100 %) for 30 days. Results: The average hair length of aquades (P0) was 5.88 ± 0.36 mm, minoxidil group 2% (P1) 7.4 ± 0.20 mm. , cinnamon essential oil group (P2) 8.32 ± 0.18 mm In the diameter size of hair follicles in the three groups showed the mean diameter size of hair follicles was significantly different (P <0.05). The average results of the group hair follicle diameter (P0) were 79.73 ± 30.68 µm, group (P1) 127.07 ± 38.20 µm and group (P2) 130.91 ± 49.96 µm. The LSD test results on hair length measurements between the three groups showed a very significant difference (P <0.01). Group P0 and P1-1,51 mm, group P0 and P2-2,44 mm group P1 and P2 0,92 mm. The results of LSD hair follicle diameter P0 and P1 -47.34 µm (P <0.05), P0 and P2 -51.18 µm (P <0.01), P1 and P2 -3.84 µm (P> 0 , 05) Conclusion: The application of topical cinnamon essential oil increases hair length and diameter size of male wistar rat hair follicles as effectively as topical minoxidil 2%.
Ashitaba (Angelica keiskei) leaves extract prevents spatial memory impairment in male balb/c mice (Mus musculus) with brain aging Indah Mira Tiaraputri Wijaya; Wimpie Pangkahila; Thomas Eko Purwata
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.64

Abstract

Background: Memory impairment is one of the symptoms of aging process which can be caused by free radicals or oxidative stress. Antioxidants such as flavonoids are compounds that can inhibit reactive oxygen species (ROS). Ashitaba (Angelica keiskei) is one of the natural compound that contains high flavonoids. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract prevents spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging Methods: Experimental randomized pretest-posttest group using 26 mice, aged 3 months, healthy (active and willing to eat), weighing 20-30 grams. Mice were induced with oral D-Galactose of 300 mg/kgBW/day for 4 consecutive weeks to achieve the brain aging condition. Control group treated with placebo (2 ml of aquabidest), and another group was treated with Ashitaba leaves extract of 20 mg/kgBW/day. Before and after treatment for 28 days, spatial memory were examined using the Morris Water Maze method. Results: The results showed a significant decrease in probe test (from 72,9±5,69% to 40,8±5,10%; p<0,001) and the number of platform crossings (from 7,37±0,527 times to 4,50 ± 0,771 times; p<0,001) in the control group suggesting that D-Galactose-only treatment impaired spatial memory. In contrast, the group treated with D-Galactose and Ashitaba leaves extract, there was no change in probe test (from 72,1±5,9% to 66,0±6,72%; p>0,05) and number of platform crossings (from 7,65±0,881 times to 6,98±0,787 times; p>0,05). Conclusion: This study indicated that Ashitaba (Angelica keiskei) leaves extracts prevented spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging.
Combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract increased pancreatic beta cells number but have no effect toward glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus) Meiyati Panambunan; Wimpie Pangkahila; Anak Agung Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.908 KB) | DOI: 10.36675/ijaam.v3i2.43

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Introduction: Diabetes mellitus is a complex and progressive disease which often lead to several debilitating complications that partly caused by by free radicals which can be overcome with antioxidants. Bay leaves contain essential oils, tannins, flavonoids and terpenoids which have considerable antioxidant properties. Therefore, this study aims to determine the effect combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract toward pancreatic beta cells density and glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus). Methods: An experimental posttest only control group study was conducted using 36 albino male rats, aged 2-3 months, weighing 180-200 grams. The rats were divided into 2 groups (n= 18) with control group treated with 1.8mg/200g body weight vildagliptin and 2cc placebo (aquabidest) while the treatment group received 1.8mg/200g BW vildagliptin and 250 mg/200g BW bay leaves extract. All treatment lasted for 21 days. Results: The results showed that pancreatic beta cell counts in the treatment group was significantly higher than the control group (109.07 ± 20.47 cells/field of view vs 90.87 ± 13.91 cells/field of view; p<0.01). However, the levels of Glycated albumin between two groups were not significantly different (treatment vs control: 17.33 ± 4.51 vs 20.18 ± 4.57; p=0.068). Conclusion: This study suggested that combination treatment of vildagliptin and bay leaf extract increased pancreatic beta cells but did not reduce glycated albumin levels in diabetic male Wistar rats.
Co-Authors ., Nuriyah ., Yulyani ., Zenitalia A. A. G. P. Wiraguna A.A. Gede Budhiarta A.A. Gede Budhiarta A.A. Gede Budhiarta A.A.G.P Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP. Wiraguna Agung Wiwiek Indrayani Agus Eka Darwinata Akpriyanti, DG Ika Aman, I G M Anak Agung Ayu Ngurah Susraini Anak Agung Gde Budhiarta Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Anak Agung Gede Budhiarta Anak Agung Gede Putra Wiraguna Andrew Lie Angelia Ongko Prabowo Anne Carolina Kencanawati Anshori, Ahmad M. Aprilany Stella peng Astried Haryanto Augus Venty Azizah, Norma Nur Azizah Bagus Komang Satriyasa Balqis Wasliati Balqis Wasliati Bissi Herliane Budhiarta, Anak A.G. Chandra, Aji B. Chynthia Friama Delly Dahlia Dessy Indriana Hatta Dewi, Dian Andriani Ratna Dian Andriani Dumaria, Corazon H. Dwindi Saptania Efrida Sumawati Erwin Sugito Eveline Margo Feily Sari Moelyono Fenni Liem Ferbian M. Siswanto, Ferbian M. Fiter, Joni Gde Ngurah Idraguna Pinatih Gina Septivani Hendrik Danico Heny Widiyowati S Hery Widijanto Huda, Siti M. N. I Gede Made Aman I Gede Widhiantara I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Ketut Suada I Ketut Tunas I Nyoman Adiputra I Nyoman Mangku Karmaya I NYOMAN MANTIK ASTAWA I Wayan Weta Ida Sri Iswari Indah Mira Tiaraputri Wijaya Ingrid Rotinsulu J. A. Pangkahila Junivan Lindra Kandy Rosa Ismalia Larasati, Veny Laura Indriana Liliana, Nize Lis Widodo Peni Liya Rosdiana Luh Putu Iin Indrayani Maker Made Ayu Mirah Wulandari Made Oka Negara MADE RATNA SARASWATI . Manus, Widya Christine Maulidha Suzi Annisa Maya Cesarya Simangunsong Meiny Linda Setiawan Meiyati Panambunan Melanie Maria Magdalena Ratulangi Mery Merysia Milhanah Milhanah Mochtar, Fransiska Muhammad Rahmatullah Ni Luh Putri Astini Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Sriwidyani Nina Marini Nining Triyana Darise Nurwiyanti, . Palumpun, Eva F. Periskila Abigail Phery Candres Putri Puspitasari R. A.T. Kuswardhani Rafiah Sirikit Saenong Riliani, Marisa Romy Kamaluddin Ronald Suryaprawira Rusmiatik Rusmiatik Sabella Setia Wardani Siahaan, Eva R. Siahaan, Marintan S. Y. Sofiana, Rahmi Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Tambor Tiur Marlina Tamon, Oktavian Tarnajaya, Karnia Thomas Eko Purwata Trisna Yuliharti Tersinanda Ursula Sulistiani Vany Novanty Vashti Saraswati Vitia Tandy Widhayanthi, Trisna A. Wiraguna AAGP Wiraguna, A A G P Wiraguna, A AGP Wiraguna, AAG Wiraguna, Anak A.G.P. Yuziana Yuziana