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Oral administration of clabet (Trigonella foenum graecum) seeds ethanol extracts improved testosteron level in old-male wistar (Rattus norvegicus) mice Bissi Herliane; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.464 KB)

Abstract

Introduction: Aging can cause decreased testosterone levels in men which is known as andropause. Some herbs are known to increase the levels Testosterone. One of them is Klabet seed that contain phyto-testosterone. The purpose of this study was to prove that the administration of ethanol extract of Klabet seeds increased testosterone levels in old male Wistar rats. Methods: A pretest-posttest control group design study was conducted using 20 male white wistar rats that were divided into 2 (two) groups: control group that received 2 cc of distilled water and treatment group that received 2 cc ethanol extract of Klabet seeds 250mg/250gBB rats/day by sonde for 14 days. The testosterone level was measured on day 1 and 15. Results: The analysis showed that there was no significant difference in the pre-test and post-test testosterone level P0 (Pre-test:16.4±0.553 ng/ml; posttest: 15.9±1.51ng/ml) while increased level of testosterone could be observed in treatment group (P1) (pre-test: 16.6±0.476ng/ml; post-test: 19.4±0.828ng/ml. Comparative analysis between post-test results (P0 vs P1) showed that the difference was statistically significant (p <0.001). Conclusion: Oral administration of Klabet seeds ethanol extract can significantly increase testosterone level in old male wistar mice.
Administration of 5% Tranexamic Acid Cream Reduce the Amount of Melanin as Effective as 4% Hydroquinone Cream in Female Guinea Pigs (Cavia porcellus) Exposed to Ultraviolet-B Light Nina Marini; Wimpie Pangkahila; AAGP. Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.31

Abstract

Introduction: Exposure to ultraviolet B light will increase the amount of melanin which is a common sign of skin aging, one of which is hyperpigmentation which causes psychological impacts, causing social disturbances. Currently, no effective treatment has been found, where hydroquinone as the gold standard provides dangerous side effects, so a more effective and safe material is needed for long-term use. The aim of this study was to prove that tranexamic acid cream was as effective as hydroquinone in reducing the amount of melanin in female guinea pigs (Cavia porcellus) exposed to UVB rays. Methods: This study used a post test only control group design with 36 adult female guinea pigs exposed to UVB rays for 6 weeks at 60mJ/cm2 three times a week. After 2 weeks the guinea pigs were randomly divided into 2 groups with 18 guinea pigs in each group. Group A was given 4% hydroquinone cream and group B was given tranexamic acid cream 5%. Exposure to UVB rays was continued as long as the guinea pig was given the cream treatment. After 42 days the guinea pigs were biopsied to determine the amount of epidermal melanin. Results: The results showed that the mean amount of melanin in the hydroquinone group was 7.40 ± 3.61 and in the tranexamic acid group was 7.27 ± 4.06 (p=0.925; p>0.05). There was no significant difference between the hydroquinone cream group and the tranexamic sour cream group. Conclusion: This study concluded that administration of 5% tranexamic acid cream reduced the amount of melanin as effective as 4% hydroquinone cream in female guinea pigs exposed to UVB light.
Administration of Oral Sorghum Seed Extract (Sorghum bicolor L.) Increased Pancreatic Beta Cells Count but did not Reduce Glycated Albumin Levels in Male Wistar Rats (Rattus Norvegicus) with Diabetes Mellitus Efrida Sumawati; Wimpie Pangkahila; A.A. Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.91

Abstract

Background: It is required a long time of diabetes mellitus treatment, such as doing general activities or even insulin. Sorghum is a plant that contains antioxidants. The aim of this research was to prove the oral administration of Sorghum Seed Extract (Sorghum bicolor L.) in increasing the pancreatic beta cells count but did not reduce the levels of glycated albumin in male Wistar rats (Rattus norvegicus) with Diabetes Mellitus. Method: This study used experimental post-test only group design. 36 male Wistar rats were used and injected with the nicotinamide and streptozotocin intraperitoneally. Rats were divided into 2 groups, the control group (n=18) was administrated with distilled water at a dose of 2cc/200g body weight of rats. The the treatment group (n=18) was administrated with ethanol extract of sorghum seeds with a dose of 80 mg/kgBW of rats/ day. The post-test of glycated albumin levels and histopathology examination of pancreatic beta cells count were performed. Results: The mean of pancreatic β cells count in the control group was 64.31 ± 19.397. It was lower than the treatment group which was 119.44 ± 33.799 (p <0.005). The mean results glycated albumin level of the research in the control group was 8.577 ± 3.105 ng/ml and in the treatment group was 9,191 ± 3,014 ng/ml (p> 0,05), therefore it was not proven. Conclusion: The administration of ethanol extract of sorghum seeds increased pancreatic β cells count, but it did not reduce glycated albumin levels in male Wistar rats (Rattus Norvegicus) with Diabetes Mellitus.
Nutrilite green trim® reduced body weight, waist circumference, and body fat percentage in obese men with moderate physical exercise and low-calorie diet Romy Kamaluddin; Wimpie Pangkahila; Alex Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.75

Abstract

Introduction: Management of obesity are including consumption of supplements. Nutrilite GreenTrim® Supplement contains green tea extract and EGCG and squalene. This study aimed to prove the effects of Nutrilite GreenTrim® on body weight, waist circumference, and body fats percentage in obese men. Methods: This clinical trial used pretest-posttest control group design in 22 obese men, aged 25-40 years with a BMI 25-35 kg/m2. Subjects were divided into the control group (K) and the treatment group (P). Subjects were given moderate physical exercise and a low-calorie diet. Results: The results showed K group lost weight (from 85.1±8.99 to 82.3±9.21 kg; p<0.001), while the P group lost weight (from 84.1±10.7 to 80.1±10.9 kg; p <0.001) and the difference showed that the P group lost weight greater than the K group (p = 0.007). K group experienced a decrease in waist circumference (from 96.9±7.69 to 93.8±8.84 cm; p <0.001), while the P group (from 96.5±7.78 to 92.2±7.59 cm; p <0.001) the difference showed that the decrease in waist circumference in the P group was better than the K group (p = 0.041). In the K group, body fat percentage decreased (from 27.5±3.31 to 25.8±3.77%; p <0.001), the P group also decreased (from 26.5±3.78 to 23.3±3.67%; p<0.001) the analysis showed that the decrease in body fat percentage in the P group was better than the K group (p <0.001). Conclusion: Supplementation of Nutrilite Green Trim® reduced body weight, waist circumference, and body fats percentage in obese men with moderate physical exercise and low-calorie diet.
Piper crotatum leaves extract reduced fasting blood glucose and glycated albumin but has lower efficacy than metformin in diabetic rats Muhammad Rahmatullah; Wimpie Pangkahila; A.A. Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.84 KB) | DOI: 10.36675/ijaam.v4i1.42

Abstract

Introduction: Piper crocatum leaves extract has been widely proven in reducing blood glucose levels in rats. However, there is still no study that compared its effectiveness to metformin. Therefore, this study aimed to evaluate the efficacy of Piper crotatum leaves extract compared to metformin in reducing fasting blood glucose and glycated albumin. Methods: Experimental pretest-posttest groups study using 16 adult male Wistar rats, aged 2-3 months, and weighing 200-220 grams was conducted. Diabetes was induced by using Streptozotocin and Nicotinamide intraperitonially. The control group was treated with metformin 9 mg/200gBB/day while the treatment group was treated by Piper crocatum leaves extract at 0.6 g/200gBB/day for 14 days. Venous blood sample was used to assess fasting blood glucose and glycated albumin. Resuts: The mean of fasting blood glucose level decreased significantly in the control group from 182.00+19.38 mg/dl to 117.38+12.51 mg/dl (p<0.001) and the levels of GA was also decreased from 3.04+0.99 ng/ml to 1.38+0.63 ng/ml (p<0.001). In the treatment group, the level of fasting blood glucose decreased from 179.50+24.39 mg/dl to 140.13+15.12 mg/dl (p<0.001) and the levels of glycated albumin was also decreased from 4.01+1.12 ng/ml to 3.58+0.90 ng/ml (p=0.036). There was no difference in the level of fasting blood glucose and GA between the two groups before treatment but both parameters were significantly lower compared to the treatment group (p=0.005 and p<0.001 respectively). Conclusion: Administration of piper crotatum leaves extract reduced fasting blood glucose but has lower efficacy compared to metformin in diabetic Wistar rats.
The administration of supplements milk with high protein of whey increased estrogen level but did not affect testosterone level in male wistar rat (Rattus norvegicus) Dian Andriani; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Supplements milk is a high protein of whey that consumed by many teenagers to maintain the fitness in accordance with the advertisements in the society, the presence of hormones in milk that are not listed in its composition causes a hormonal balance disorder. The aim of this research was to prove that giving supplements milk with a high protein of whey in increasing estrogen levels and lowers testosterone counts in male Wistar rats (Rattus norvegicus). Methods: This research employed experimental research using a pretest-posttest control group design that used 22 white male Wistar rats (Rattus norvegicus), the samples were divided into 2 groups, namely the control group (P0) and the treatment group (P1) that treated by supplements milk with a high-protein of whey once a day for three weeks. The blood was taken before and after treatment for estrogen levels and testosterone counts. Result: The finding indicated that a significant increase in estrogen levels before and after the administration of milk supplement with high whey protein 11.603 ± 0.502 ng/ ml to 16.141 ± 0.347 ng/ ml (p<0.001). There was no decrease in testosterone counts before and after subjects were administered by milk supplement with high whey protein 1.86 ± 0.325 ng/ ml to 1.69 ± 0.260ng/ ml p = 0.220 (p> 0.05). Conclusion: Based on the results of the analysis it could be concluded that the administration of milk supplement with high whey protein could increase estrogen levels while decreasing testosterone in male Wistar rats (Rattus norvegicus).
Soymilk Formula Increases Estrogen and Reduces Testosterone Level in Male Infant White Wistar Rats Eveline Margo; Wimpie Pangkahila; I Gusti Made Aman
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.426 KB) | DOI: 10.21705/mcbs.v3i1.40

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Background: Children’s immunity system is relatively lower on first year of life, causing atopic babies, leading to allergy. Ideally, newborn babies are given breast milk as main food source on the first 6 months, but often being replaced with cow milk which can induce Cow’s Milk Protein Allergy. For the alternative can replace it with soymilk formula which contains phytoestrogen from isoflavon, which works just like estrogen. The aim of this study to determine whether administration of soymilk formula is capable on increasing estrogen level and reducing testosterone level on male infant white Wistar rats (Rattus norvegicus).Materials and Methods: This research used post test only control group design. Samples were consisted of 36 infant male white Wistar rats aged 7 days, weighing from 10-15 grams, divided into 2 groups, control group which was given cow’s milk and intervention group which was given soymilk. Each group were given intervention with the dose 5% of BW (grams), given twice-a-day orally for 21 days, and subsequently in the morning their blood samples were taken to examine the level of estrogen and testosterone hormone.Results: The study showed that the level of estrogen on intervention group was significantly higher than the control group with mean estrogen level of 0.55±0.03 pg/mL in the control group and 0.82±0.01 pg/mL in the intervention group, while the mean testosterone level was significantly lower on intervention group compared with control group (p<0.05) with 3.25±0.15 ng/mL on control group and 2.36±0.22 ng/mL on intervention group.Conclusion: Soymilk was able to increase estrogen level and reduce testosterone level on male infant white Wistar rats (Rattus norvegicus).Keywords: soymilk formula, estrogen, testosterone, male infant rats
ASTAXANTHIN MENGHAMBAT PENURUNAN JUMLAH SEL LEYDIG DAN KADAR TESTOSTERON PADA TIKUS WISTAR JANTAN YANG DIPAPAR DENGAN ASAP ROKOK Andrew Lie; Wimpie Pangkahila; L.P. Iin Indrayani Maker
IJCNP : INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN Vol 3 No 1 (2020): IJCNP (INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN)
Publisher : Perhimpunan Dokter Gizi Klinik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54773/ijcnp.v3i1.42

Abstract

Background. The exposure of cigarette smoke impacts negatively on Leydig cell count and testosterone level. Astaxanthin is known for its ability to neutralize free radicals far more potent than any other kind of antioxidant. Research objectives. This research aims to prove the effect of Astaxanthin in inhibiting decrease of Leydig cell count and testosterone level in male Wistar rats exposed with cigarette smoke. Methodology. The posttest only control group study design was conducted on 36 male Wistar rats, 12-16 weeks in age, with 200-210 grams body weight. Samples were randomly divided into two groups, consisting of a control group exposed with cigarette smoke and 0.5 ml of aquadest and a study group exposed with cigarette smoke and 0.1 mg of astaxanthin/200 gr BW daily for 30 days. On day 31, blood samples were taken to measure the testosterone level. Both testes were taken for Leydig cells count assessment. Comparative analysis was done to see any significant difference between the study and control group. Research results. The results show that the mean number of Leydig cells and testosterone levels in the study group was significantly higher than the control group (p<0.01). Conclusion. Oral astaxanthin administration inhibited the decrease of Leydig cell count and testosterone level in male Wistar rats exposed with cigarette smoke. Key words: Astaxanthin, Testosterone, Leydig Cell, Andropause
IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS) Widya Christine Manus; Wimpie Pangkahila; I Gusti Made Aman
Berkala Ilmiah Kedokteran Duta Wacana Vol 4, No 2 (2019): BERKALA ILMIAH KEDOKTERAN DUTA WACANA
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3423.507 KB) | DOI: 10.21460/bikdw.v4i2.150

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Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/L) compared to the control group (3.091.33x103/L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/L) compared to the control group (6.70 ± 1.96x103/L), with p
The administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and did not increase estrogen hormone level in female albino rat (Rattus norvegicus) Wistar strain Sabella; Wimpie Pangkahila; Anak Agung Ayu Ngurah Susraini
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.146

Abstract

Background: This study aimed to prove that administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and estrogen levels in female albino rat (Rattus norvegicus) Wistar strain. Methods: This research was a true experimental with randomized pretest-posttest control group design. The research subjects were 18-female-albino-rats aged 21-days, weight 25-28 grams, divided into 2-groups: control group which was given standard food and sugar solution (0.36 grams of sugar dissolved in 1 ml aquadest), and intervention group which was given standard food and UltraMimi Kids® UHT milk 1 ml for the first 14-days and upgraded to 2 ml for the rest 14-days, given three times a day for 28-days. The rats body weight and estrogen levels were measured using Tanita-KD-192-digital-scales and ELISA method. Results: The control group had an increased body weight (26.2±1.2-66.1±5.94 grams; p<0,001), but there was no change in estrogen levels (38.59±11.19-44.59±5.42 ng/L; p=0.217). Whereas, the intervention group had an increased body weight (25.77±0.83-85.33±10.24 grams; p=0.008), but there was no change in estrogen levels (39.91±3.73-42.66±2.24 ng/L; p=0.066). After 28-days of intervention (posttest), it was found that the mean of body weight in the intervention group was higher than the control group (p<0.001), there was no significant difference in estrogen levels (p=0.339) and food intake from the remaining feed in both groups (0.51±0.81-0.36±0.55 grams; p=0.823). Conclusion: The administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and did not increase estrogen hormone level in female albino rat (Rattus norvegicus) Wistar strain.
Co-Authors ., Nuriyah ., Yulyani ., Zenitalia A. A. G. P. Wiraguna A.A. Gede Budhiarta A.A. Gede Budhiarta A.A. Gede Budhiarta A.A.G.P Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP. Wiraguna Agung Wiwiek Indrayani Agus Eka Darwinata Akpriyanti, DG Ika Aman, I G M Anak Agung Ayu Ngurah Susraini Anak Agung Gde Budhiarta Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Anak Agung Gede Budhiarta Anak Agung Gede Putra Wiraguna Andrew Lie Angelia Ongko Prabowo Anne Carolina Kencanawati Anshori, Ahmad M. Aprilany Stella peng Astried Haryanto Augus Venty Azizah, Norma Nur Azizah Bagus Komang Satriyasa Balqis Wasliati Balqis Wasliati Bissi Herliane Budhiarta, Anak A.G. Chandra, Aji B. Chynthia Friama Delly Dahlia Dessy Indriana Hatta Dewi, Dian Andriani Ratna Dian Andriani Dumaria, Corazon H. Dwindi Saptania Efrida Sumawati Erwin Sugito Eveline Margo Feily Sari Moelyono Fenni Liem Ferbian M. Siswanto, Ferbian M. Fiter, Joni Gde Ngurah Idraguna Pinatih Gina Septivani Hendrik Danico Heny Widiyowati S Hery Widijanto Huda, Siti M. N. I Gede Made Aman I Gede Widhiantara I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Ketut Suada I Ketut Tunas I Nyoman Adiputra I Nyoman Mangku Karmaya I NYOMAN MANTIK ASTAWA I Wayan Weta Ida Sri Iswari Indah Mira Tiaraputri Wijaya Ingrid Rotinsulu J. A. Pangkahila Junivan Lindra Kandy Rosa Ismalia Larasati, Veny Laura Indriana Liliana, Nize Lis Widodo Peni Liya Rosdiana Luh Putu Iin Indrayani Maker Made Ayu Mirah Wulandari Made Oka Negara MADE RATNA SARASWATI . Manus, Widya Christine Maulidha Suzi Annisa Maya Cesarya Simangunsong Meiny Linda Setiawan Meiyati Panambunan Melanie Maria Magdalena Ratulangi Mery Merysia Milhanah Milhanah Mochtar, Fransiska Muhammad Rahmatullah Ni Luh Putri Astini Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Sriwidyani Nina Marini Nining Triyana Darise Nurwiyanti, . Palumpun, Eva F. Periskila Abigail Phery Candres Putri Puspitasari R. A.T. Kuswardhani Rafiah Sirikit Saenong Riliani, Marisa Romy Kamaluddin Ronald Suryaprawira Rusmiatik Rusmiatik Sabella Setia Wardani Siahaan, Eva R. Siahaan, Marintan S. Y. Sofiana, Rahmi Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Tambor Tiur Marlina Tamon, Oktavian Tarnajaya, Karnia Thomas Eko Purwata Trisna Yuliharti Tersinanda Ursula Sulistiani Vany Novanty Vashti Saraswati Vitia Tandy Widhayanthi, Trisna A. Wiraguna AAGP Wiraguna, A A G P Wiraguna, A AGP Wiraguna, AAG Wiraguna, Anak A.G.P. Yuziana Yuziana