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All Journal INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES E-Jurnal Medika Udayana Jurnal e-Biomedik JURNAL BIOMEDIK Prosiding Seminar Nasional MIPA Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya WMJ (Warmadewa Medical Journal) Molecular and Cellular Biomedical Sciences (MCBS) Berkala Ilmiah Kedokteran Duta Wacana ISM (Intisari Sains Medis) : Jurnal Kedokteran Majalah Kesehatan Pharmamedika Jurnal Kedokteran: Media Informasi Ilmu Kedokteran dan Kesehatan Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Neurologico Spinale Medico Chirurgico Indonesian Journal of Global Health research IJAAM (Indonesian Journal of Anti-Aging Medicine) IJCNP (INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN) Indonesian Archives of Biomedical Research (InABR) Cermin Dunia Kedokteran Eduvest - Journal of Universal Studies Asian Journal of Social and Humanities Cermin Dunia Kedokteran
Wimpie I Pangkahila
Department Of Andrologi And Sexology, Faculty Of Medicine, Udayana University
Aerospace Engineering Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Piper crotatum leaves extract reduced fasting blood glucose and glycated albumin but has lower efficacy than metformin in diabetic rats Muhammad Rahmatullah; Wimpie Pangkahila; A.A. Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.84 KB) | DOI: 10.36675/ijaam.v4i1.42

Abstract

Introduction: Piper crocatum leaves extract has been widely proven in reducing blood glucose levels in rats. However, there is still no study that compared its effectiveness to metformin. Therefore, this study aimed to evaluate the efficacy of Piper crotatum leaves extract compared to metformin in reducing fasting blood glucose and glycated albumin. Methods: Experimental pretest-posttest groups study using 16 adult male Wistar rats, aged 2-3 months, and weighing 200-220 grams was conducted. Diabetes was induced by using Streptozotocin and Nicotinamide intraperitonially. The control group was treated with metformin 9 mg/200gBB/day while the treatment group was treated by Piper crocatum leaves extract at 0.6 g/200gBB/day for 14 days. Venous blood sample was used to assess fasting blood glucose and glycated albumin. Resuts: The mean of fasting blood glucose level decreased significantly in the control group from 182.00+19.38 mg/dl to 117.38+12.51 mg/dl (p<0.001) and the levels of GA was also decreased from 3.04+0.99 ng/ml to 1.38+0.63 ng/ml (p<0.001). In the treatment group, the level of fasting blood glucose decreased from 179.50+24.39 mg/dl to 140.13+15.12 mg/dl (p<0.001) and the levels of glycated albumin was also decreased from 4.01+1.12 ng/ml to 3.58+0.90 ng/ml (p=0.036). There was no difference in the level of fasting blood glucose and GA between the two groups before treatment but both parameters were significantly lower compared to the treatment group (p=0.005 and p<0.001 respectively). Conclusion: Administration of piper crotatum leaves extract reduced fasting blood glucose but has lower efficacy compared to metformin in diabetic Wistar rats.
The administration of supplements milk with high protein of whey increased estrogen level but did not affect testosterone level in male wistar rat (Rattus norvegicus) Dian Andriani; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.097 KB)

Abstract

Introduction: Supplements milk is a high protein of whey that consumed by many teenagers to maintain the fitness in accordance with the advertisements in the society, the presence of hormones in milk that are not listed in its composition causes a hormonal balance disorder. The aim of this research was to prove that giving supplements milk with a high protein of whey in increasing estrogen levels and lowers testosterone counts in male Wistar rats (Rattus norvegicus). Methods: This research employed experimental research using a pretest-posttest control group design that used 22 white male Wistar rats (Rattus norvegicus), the samples were divided into 2 groups, namely the control group (P0) and the treatment group (P1) that treated by supplements milk with a high-protein of whey once a day for three weeks. The blood was taken before and after treatment for estrogen levels and testosterone counts. Result: The finding indicated that a significant increase in estrogen levels before and after the administration of milk supplement with high whey protein 11.603 ± 0.502 ng/ ml to 16.141 ± 0.347 ng/ ml (p<0.001). There was no decrease in testosterone counts before and after subjects were administered by milk supplement with high whey protein 1.86 ± 0.325 ng/ ml to 1.69 ± 0.260ng/ ml p = 0.220 (p> 0.05). Conclusion: Based on the results of the analysis it could be concluded that the administration of milk supplement with high whey protein could increase estrogen levels while decreasing testosterone in male Wistar rats (Rattus norvegicus).
Soymilk Formula Increases Estrogen and Reduces Testosterone Level in Male Infant White Wistar Rats Eveline Margo; Wimpie Pangkahila; I Gusti Made Aman
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.426 KB) | DOI: 10.21705/mcbs.v3i1.40

Abstract

Background: Children’s immunity system is relatively lower on first year of life, causing atopic babies, leading to allergy. Ideally, newborn babies are given breast milk as main food source on the first 6 months, but often being replaced with cow milk which can induce Cow’s Milk Protein Allergy. For the alternative can replace it with soymilk formula which contains phytoestrogen from isoflavon, which works just like estrogen. The aim of this study to determine whether administration of soymilk formula is capable on increasing estrogen level and reducing testosterone level on male infant white Wistar rats (Rattus norvegicus).Materials and Methods: This research used post test only control group design. Samples were consisted of 36 infant male white Wistar rats aged 7 days, weighing from 10-15 grams, divided into 2 groups, control group which was given cow’s milk and intervention group which was given soymilk. Each group were given intervention with the dose 5% of BW (grams), given twice-a-day orally for 21 days, and subsequently in the morning their blood samples were taken to examine the level of estrogen and testosterone hormone.Results: The study showed that the level of estrogen on intervention group was significantly higher than the control group with mean estrogen level of 0.55±0.03 pg/mL in the control group and 0.82±0.01 pg/mL in the intervention group, while the mean testosterone level was significantly lower on intervention group compared with control group (p<0.05) with 3.25±0.15 ng/mL on control group and 2.36±0.22 ng/mL on intervention group.Conclusion: Soymilk was able to increase estrogen level and reduce testosterone level on male infant white Wistar rats (Rattus norvegicus).Keywords: soymilk formula, estrogen, testosterone, male infant rats
ASTAXANTHIN MENGHAMBAT PENURUNAN JUMLAH SEL LEYDIG DAN KADAR TESTOSTERON PADA TIKUS WISTAR JANTAN YANG DIPAPAR DENGAN ASAP ROKOK Andrew Lie; Wimpie Pangkahila; L.P. Iin Indrayani Maker
IJCNP : INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN Vol 3 No 1 (2020): IJCNP (INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN)
Publisher : Perhimpunan Dokter Gizi Klinik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54773/ijcnp.v3i1.42

Abstract

Background. The exposure of cigarette smoke impacts negatively on Leydig cell count and testosterone level. Astaxanthin is known for its ability to neutralize free radicals far more potent than any other kind of antioxidant. Research objectives. This research aims to prove the effect of Astaxanthin in inhibiting decrease of Leydig cell count and testosterone level in male Wistar rats exposed with cigarette smoke. Methodology. The posttest only control group study design was conducted on 36 male Wistar rats, 12-16 weeks in age, with 200-210 grams body weight. Samples were randomly divided into two groups, consisting of a control group exposed with cigarette smoke and 0.5 ml of aquadest and a study group exposed with cigarette smoke and 0.1 mg of astaxanthin/200 gr BW daily for 30 days. On day 31, blood samples were taken to measure the testosterone level. Both testes were taken for Leydig cells count assessment. Comparative analysis was done to see any significant difference between the study and control group. Research results. The results show that the mean number of Leydig cells and testosterone levels in the study group was significantly higher than the control group (p<0.01). Conclusion. Oral astaxanthin administration inhibited the decrease of Leydig cell count and testosterone level in male Wistar rats exposed with cigarette smoke. Key words: Astaxanthin, Testosterone, Leydig Cell, Andropause
IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS) Widya Christine Manus; Wimpie Pangkahila; I Gusti Made Aman
Berkala Ilmiah Kedokteran Duta Wacana Vol 4, No 2 (2019): BERKALA ILMIAH KEDOKTERAN DUTA WACANA
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3423.507 KB) | DOI: 10.21460/bikdw.v4i2.150

Abstract

Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/L) compared to the control group (3.091.33x103/L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/L) compared to the control group (6.70 ± 1.96x103/L), with p
The administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and did not increase estrogen hormone level in female albino rat (Rattus norvegicus) Wistar strain Sabella; Wimpie Pangkahila; Anak Agung Ayu Ngurah Susraini
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.146

Abstract

Background: This study aimed to prove that administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and estrogen levels in female albino rat (Rattus norvegicus) Wistar strain. Methods: This research was a true experimental with randomized pretest-posttest control group design. The research subjects were 18-female-albino-rats aged 21-days, weight 25-28 grams, divided into 2-groups: control group which was given standard food and sugar solution (0.36 grams of sugar dissolved in 1 ml aquadest), and intervention group which was given standard food and UltraMimi Kids® UHT milk 1 ml for the first 14-days and upgraded to 2 ml for the rest 14-days, given three times a day for 28-days. The rats body weight and estrogen levels were measured using Tanita-KD-192-digital-scales and ELISA method. Results: The control group had an increased body weight (26.2±1.2-66.1±5.94 grams; p<0,001), but there was no change in estrogen levels (38.59±11.19-44.59±5.42 ng/L; p=0.217). Whereas, the intervention group had an increased body weight (25.77±0.83-85.33±10.24 grams; p=0.008), but there was no change in estrogen levels (39.91±3.73-42.66±2.24 ng/L; p=0.066). After 28-days of intervention (posttest), it was found that the mean of body weight in the intervention group was higher than the control group (p<0.001), there was no significant difference in estrogen levels (p=0.339) and food intake from the remaining feed in both groups (0.51±0.81-0.36±0.55 grams; p=0.823). Conclusion: The administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and did not increase estrogen hormone level in female albino rat (Rattus norvegicus) Wistar strain.
Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed Guin Chynthia Friama; Anak Agung Gde Putra Wiraguna; Wimpie Pangkahila
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.147

Abstract

Background: Excessive sun exposure results in increased activity of the tyrosinase enzyme and the amount of melanin in the skin which causes hyperpigmentation, a sign of premature aging. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin and tyrosinase enzymes as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin. Methods: This study used a randomized post-test only control group design. The subjects were 36 male guinea pigs (Cavia porcellus). Subjects were divided into two groups, the hydroquinone cream 4% group and the Ashitaba leaves extract cream 8% group exposed to UVB light. Skin samples were collected 48 hours after the last exposure to measure the tyrosinase enzyme levels using the ELISA method and the amount of melanin was examined by Masson-Fontana staining. Results: The mean levels of tyrosinase in the hydroquinone cream 4% group was 19.51 ± 5.16 ng/L and the Ashitaba leaves extract cream 8% group was 23.76 ± 3.09 ng/L (p = 0.005). The mean amount of melanin in the hydroquinone cream 4% group was 2.98 ± 2.27% and the Ashitaba leaves extract cream 8% group was 4.77 ± 3.33% (p = 0.069). Conclusion: The administration of Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin.
Administration of ethanol extract of Bajakah tampala (Spatholobus littoralis Hassk) stem decreased reactive oxygen species, visceral fat and body weight of obese rats Vany Novanty; Wimpie Pangkahila; Ni Nyoman Ayu Dewi
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.150

Abstract

Background: Oxidative stress plays a role in the obesity mechanism, thus leads to premature aging. High antioxidant capacity in Bajakah tampala stem may effectively lessen oxidative stress and reduce fat mass and body weight accordingly. This study aimed to provide Bajakah tampala stem extract's effect in lowering ROS level, visceral fat weight, and overall weight of obese male Wistar rats. Method: A true experimental design was conducted on male Wistar rats aged 2-3 months with obesity. Thirty-two obese rats were evenly divided into a placebo group and a group given Bajakah tampala stem extract, with 16 rats in each group. For 28 days, both groups were fed a high-fat diet. The subject body weights were weighed every week. ROS levels and visceral fat weight were evaluated after the intervention was done. Comparative analysis between groups was performed. Results: The results showed mean levels of ROS (56.2 ± 7.4 U/ml vs. 400.9 ± 50.7 U/ml; p < 0.001), visceral fat weight (2.6 ± 0.2 g vs. 3.4 ± 0.9 g; p < 0.001), and the final body weight (241.5 ± 2.8 g vs. 261.5 ± 13.8 g; p < 0.001) were significantly lower in the study group than the control group. Conclusion: The study indicates Bajakah tampala stem extract administration effectively reduced ROS levels, visceral fat weight, and body weight in obese male Wistar rats.
Oral administration of Bali Robusta coffee (Coffea canephora) extract prevented the reduction of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training Kandy Rosa Ismalia; Wimpie Pangkahila; Ni Putu Sriwidyani
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.151

Abstract

Background: Excessive exercise cause a deterioration of organ function and structure. Overtraining will cause reduce antioxidant reserve and excess free radical production. In this condition, there will be a decrease in the number of Leydig cells in the testis and followed by a decrease in testosterone level. Bali robusta coffee extract contains active compounds such as alkaloids, saponins, flavonoids, tannins and polyphenols. This study aims to prove whether Bali robusta coffee extract (Coffea canephora) can prevent a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training. Methods: Thirty-six rats were randomly divided into 2 groups: the control group (placebo aquabidest 2 ml) and the treatment group (Bali robusta coffee extract of 1 gram/kg BW). Both groups received excessive physical training in the form of swimming until the subjects experienced fatigue for 21-days. Results: The results showed that the mean of Leydig cell number in the treatment group was significantly higher, which was 3.70 ± 0.89 cell/field of view, compared to the control group, which was 2.92 ± 0.65 cell/field of view (p = 0.005). The mean testosterone level in the treatment group was also significantly higher, which was 6.32 ± 0.21 nmol/ml, compared to the control group, which was 1.95 ± 0.32 nmol/ml (p < 0.001). Conclusion: The administration of Bali robusta coffee extract (Coffea canephora) prevented a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training.
Intradermal injection of umbilical cord mesenchymal stem cell was more effective than platelet rich plasma in increasing amount of collagen and fibroblasts in Wistar rats (Rattus norvegicus) back skin exposed to ultraviolet B rays Anne Carolina Kencanawati; Wimpie Pangkahila; Anak Agung Gde Putra Wiraguna
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.152

Abstract

Background: Ultraviolet B exposure is one of the causes of extrinsic skin aging. Ultraviolet exposure may lead to skin collagen damage. This study about mesenchymal stem cells from umbilical cord (UCMSC) compared with platelet rich plasma (PRP) with the aim was to prove that the intradermal injection of UCMSC was more effective than PRP in increasing amount of collagen and fibroblasts in Wistar rats' back skin exposed to Ultraviolet B rays. Methods: This study used post-test only control group design. Subjects were 36 Wistar rats aged 2.5 months. A control group consisting of 18 Wistar rats treated with intradermal injection of PRP and the treatment group with UCMSC. Both groups were exposed to UVB with a total radiation dose of 840 mJ/cm2. The parameter of collagen was determined by picro sirius red staining while fibroblasts by Haematoxylin-Eosin staining. Result: The mean amount of collagen in the control group was lower (57,82±6.52%) than the treatment group (65.69±4.51%) with p<0.001. The result showed there were differences in the number of collagen in both groups (p<0.05). The mean number of fibroblasts in the control group was lower (22.63±5.98 cell/field of view) than the treatment group (28.20±8.90 cell/field of view) with p=0.034. The result showed there were differences in mean of fibroblasts in both groups (p<0.05). Conclusion: It can be concluded that the intradermal injection of UCMSC was more effective in increasing the amount of collagen and fibroblasts than intradermal injection of PRP in Wistar rats' back skin exposed to UVB rays.
Co-Authors ., Nuriyah ., Yulyani ., Zenitalia A. A. G. P. Wiraguna A.A. Gede Budhiarta A.A. Gede Budhiarta A.A. Gede Budhiarta A.A.G.P Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna A.A.G.P. Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP Wiraguna AAGP. Wiraguna Agung Wiwiek Indrayani Agus Eka Darwinata Akpriyanti, DG Ika Aman, I G M Anak Agung Ayu Ngurah Susraini Anak Agung Gde Budhiarta Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Anak Agung Gede Budhiarta Anak Agung Gede Putra Wiraguna Andrew Lie Angelia Ongko Prabowo Anne Carolina Kencanawati Anshori, Ahmad M. Aprilany Stella peng Astried Haryanto Augus Venty Azizah, Norma Nur Azizah Bagus Komang Satriyasa Balqis Wasliati Balqis Wasliati Bissi Herliane Budhiarta, Anak A.G. Chandra, Aji B. Chynthia Friama Delly Dahlia Dessy Indriana Hatta Dewi, Dian Andriani Ratna Dian Andriani Dumaria, Corazon H. Dwindi Saptania Efrida Sumawati Erwin Sugito Eveline Margo Feily Sari Moelyono Fenni Liem Ferbian M. Siswanto, Ferbian M. Fiter, Joni Gde Ngurah Idraguna Pinatih Gina Septivani Hendrik Danico Heny Widiyowati S Hery Widijanto Huda, Siti M. N. I Gede Made Aman I Gede Widhiantara I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Ketut Suada I Ketut Tunas I Nyoman Adiputra I Nyoman Mangku Karmaya I NYOMAN MANTIK ASTAWA I Wayan Weta Ida Sri Iswari Indah Mira Tiaraputri Wijaya Ingrid Rotinsulu J. A. Pangkahila Junivan Lindra Kandy Rosa Ismalia Laura Indriana Liliana, Nize Lis Widodo Peni Liya Rosdiana Luh Putu Iin Indrayani Maker Made Ayu Mirah Wulandari Made Oka Negara MADE RATNA SARASWATI . Manus, Widya Christine Maulidha Suzi Annisa Maya Cesarya Simangunsong Meiny Linda Setiawan Meiyati Panambunan Melanie Maria Magdalena Ratulangi Mery Merysia Milhanah Milhanah Mochtar, Fransiska Muhammad Rahmatullah Ni Luh Putri Astini Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Sriwidyani Nina Marini Nining Triyana Darise Nurwiyanti, . Palumpun, Eva F. Periskila Abigail Phery Candres Putri Puspitasari R. A.T. Kuswardhani Rafiah Sirikit Saenong Riliani, Marisa Romy Kamaluddin Ronald Suryaprawira Rusmiatik, Rusmiatik Sabella Setia Wardani Siahaan, Eva R. Siahaan, Marintan S. Y. Sofiana, Rahmi Suarni . Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Tambor Tiur Marlina Tamon, Oktavian Tarnajaya, Karnia Thomas Eko Purwata Trisna Yuliharti Tersinanda Ursula Sulistiani Vany Novanty Vashti Saraswati Veny Larasati Vitia Tandy Widhayanthi, Trisna A. Wiraguna AAGP Wiraguna, A A G P Wiraguna, A AGP Wiraguna, AAG Wiraguna, Anak A.G.P. Yuziana Yuziana