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The administration 3% Tamarind (Tamarindus indica) leaves ethanol extract cream inhibited the increase of tyrosinase expression and the amount of skin melanin in male Guinea pigs (Cavia porcellus) skin exposed to UV B Angelia Ongko Prabowo; Wimpie Pangkahila; Ni Putu Sriwidyani
Neurologico Spinale Medico Chirurgico Vol 4 No 3 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i3.181

Background: The main factor of skin aging is exposure to sunlight, especially UV B rays. Tamarind leaves extract known containing tannins, flavonoids, and polyphenols as anti-aging agents. This study was aimed to prove the protective effect of 3% Tamarind leaves (Tamarindus indica) ethanol extract cream in inhibiting the skin aging due to exposure to UV B by evaluate the tyrosinase expression and the amount of melanin. Methods: This was a randomized post-test only control group design involving 36 subjects of male Guinea pigs, that divided into 2 groups. The control group received placebo cream and the treatment group received 3% Tamarind leaves ethanol extract cream. Cream application was given twice a day for 2 weeks. UV B were given 3 times a week for 2 weeks with a total dose of 390 mJ/cm2. Tyrosinase expression was examined by calculating the cell percentage that express the enzyme using the immunohistochemical method. The amount of melanin was examined digitally by calculating the pixel percentage of melanin in Masson Fontana staining slides. Statistical analysis by the Independent T-test to compare the mean between two groups with p=0.05 significance. Results: The mean tyrosinase expression and the mean amount of melanin in the control group was higher than treatment group. 20.650±4.911% vs 16.067±3.451%, p=0.003 and 10.461±4.916 pixel% vs 7.300±3.111 pixel%, p=0.029, respectively. Conclusion: Administration of 3% Tamarind leaves (Tamarindus indica) etanol extract cream inhibited the increase in tyrosinase expression and the amount of skin melanin in male guinea pigs (Cavia porcellus) exposed to UV B.

Administration of Bajakah (Spatholobus littoralis hassk) stem ethanol extract cream inhibited the increasing of mmp-1 expression and the reducing of collagen in male wistar rats (Rattus norvegicus) exposed to ultraviolet b Milhanah Milhanah; Wimpie Pangkahila; Anak Agung Gde Putra Wiraguna; IGA Wiwiek Indrayani; IM Agus Eka Darwinata; I Wayan Weta
Indonesian Archives and Biomedical Sciences Vol 1 No 1 (2021): Indonesian Archives and Biomedical Sciences Vol 1(1): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Background: Exposure to ultraviolet B (UV-B) rays causes skin aging characterized through increased free radical damaged. To prevent skin aging due to UV-B exposure, it is necessary to additionally provide antioxidants as a measure to inhibit the skin aging process. Bajakah tree (Spatholobus littoralis hassk) is one antioxidants sourche such as phenols, flavonoids, and tannins. The aim was to prove that the administration of Bajakah stem ethanol extract cream inhibits increasing MMP-1 expression and reduction of collagen number in male Wistar rats exposed to UV-B. Methods: A posttest control group study design was coducted in male rats (Rattus norvegicus), Wistar strain, aged 2-3 months, weigh 180-200gram which was divided randomly into two groups. The control group (n = 18) was exposed to UV-B rays and placebo cream (0.2 mg/cm2), while the treatment group (n = 18) was exposed to UV-B rays and Bajakah stem ethanol extract cream 15 % (0.2 mg/cm2). Both creams have aplied twice before and after exposure to UV-B rays (3 times a week, with 840 mJ/cm2) for 4 weeks. Twenty-four hours after the last day intervention, a punch biopsy of skin tissue was prepared for histological examination followed by immunohistochemical assay (for MMP-1) and Sirius red (for collagen) staining. Results: The expression of MMP-1 was significantly higher in the control group (24.3 ± 6.20%) compared to the treatment group (11.5 ± 3.21%), p<0.001. Furthermore, the number of collagen was statistically lower in the control group (63.1 ± 3.94%) compared to the treatment group (82.0 ± 3.02%), p<0.001. Conclusion: The administration of bajakah (Spatholobus littoralis hassk) stem ethanol extract cream inhibited the increasing of MMP-1 expression and the reducing of collagen cells in male Wistar rats (Rattus norvegicus) those were exposed to UV- B.

Krim Ekstrak Bawang Hitam (Allium sativum Linn) Mencegah Peningkatan Ekspresi MMP-1 dan Penurunan Jumlah Kolagen Dermis Kulit Tikus (Rattus norvegicus) Wistar Jantan yang Dipapar Sinar UV-B Balqis Wasliati; Wimpie Pangkahila; A.A.G.P. Wiraguna
Cermin Dunia Kedokteran Vol 46, No 8 (2019): Kesehatan Anak
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v46i8.444

Bawang hitam berasal dari bawang putih (Allium sativum Linn) melalui proses pemanasan sehingga terjadi peningkatan kandungan antioksidan: fenolik, flavonoid (quarcetin) dan DPPH (IC-50). Tujuan penelitian ini adalah membuktikan krim ekstrak bawang hitam (Allium sativum Linn) mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis pada kulit tikus (Rattus norvegicus) Wistar jantan yang dipapar sinar UV-B. Metode: Penelitan ini adalah eksperimental Post Test Only Control Group Design. Sampel penelitian ini 36 ekor tikus (Rattus norvegicus) Wistar jantan, dibagi menjadi 2 kelompok perlakuan secara acak. Tikus diberi bahan dasar krim dan dipapar sinar UV-B disebut kelompok kontrol, tikus diberi krim ekstrak bawang hitam 15% dan dipapar sinar UV-B disebut kelompok perlakuan. Total dosis UV-B 840 mJ/cm2 selama 4 minggu. Setelah perlakuan tikus dieuthanasia dengan ketamine-xylazine lalu ekspresi MMP-1 diperiksa secara imunohistokimia dan jumlah kolagen dermis kulitnya menggunakan pewarnaan Pico-Sirius-Red. Hasil: Rerata ekspresi MMP-1 kelompok kontrol 28,58% dan kelompok perlakuan 8,60%; p<0,001 (uji t independent); terdapat perbedaan bermakna ekspresi MMP-1 antara kelompok kontrol dan kelompok perlakuan. Rerata jumlah kolagen kelompok kontrol 57,53% dan kelompok perlakuan 83,80%; p<0,001(uji t independent); terdapat perbedaan bermakna jumlah kolagen antara kelompok kontrol dan kelompok perlakuan.Simpulan: Krim ekstrak bawang hitam (Allium sativum Linn) 15% mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis kulit tikus (Rattus norvegicus) Wistar jantan yang dipapar sinar UV-B.Black garlic produced from garlic (Allium sativum Linn) through the heating process to increase the antioxidant content: phenolic, flavonoid (quarcetin) and DPPH (IC-50). The aim of this study was to prove that black garlic extract cream (Allium sativum Linn) application prevents MMP-1 expression increase and dermal collagen decrease in male Wistar rat (Rattus norvegicus) skin exposed to UV-B light. Method: An experimental research using Post Test Only Control Group Design. Total samples of 36 male Wistar rats (Rattus norvegicus) were divided into 2 groups. Control group were given cream and UV-B exposure (P0), treatment group were given 15% black garlic cream extract and UV-B exposure (P1). The total dose of UV-B was 840mJ/cm2 for 4 weeks. The rats were euthanized with ketamine-xylazine after treatment, MMP-1 expression was examined using Immunohistochemistry and dermal collagen using Pico-Sirius-Red staining. Result: MMP-1 expression of control group 28.58% and of treatment group 8.60% (independent t test, p<0.001). Mean of collagen in control group 57.53% and in treatment group 83.8% (independent t test, p<0.001). Conclusion: Black garlic extract cream 15% prevented the increase of MMP-1 expression and the decrease of dermal collagen in male Wistar rat (Rattus norvegicus) skin exposed to UV-B light.

Pemberian deer placenta secara oral meningkatkan kadar hormon testosteron pada tikus Wistar (Rattus norvegicus) jantan muda I Made Oka Negara; Wimpie Pangkahila; I Gusti Made Aman; Alex Pangkahila; I Wayan Weta; Ni Nyoman Ayu Dewi
Intisari Sains Medis Vol. 11 No. 2 (2020): (Available online: 1 August 2020)
Publisher : DiscoverSys Inc.

Background: Men who experience hypogonadal symptoms are increasingly experienced by a younger age. This is due to a decrease in the hormone testosterone. Efforts to increase the hormone testosterone in hypogonadal men are often made to restore sexual function, libido, muscle mass, physical strength, bone density, and feelings of pleasure. This study aims to determine the effect of giving deer placenta orally in increasing levels of the hormone testosterone in young male Wistar rats (Rattus norvegicus).Methods: The experimental study was conducted using a pretest and posttest control group design involving 18 young male Wistar rats aged 2.5-3 months with a bodyweight of 150-180 grams. Wistar rats were divided into two groups, each totaling 9 rats, one group as a control group (giving placebo for 28 days) and the treatment group (giving deer placenta orally as much as 10.5 grams per day for 28 days). The data obtained were analyzed by SPSS version 20 for Windows.Results: The results showed that there was a significant increase in testosterone levels both before (3.018Â±0.282 ng/ml) and after (4.516Â±0.796 ng/ml) giving deer placenta orally as much as 10.5 grams for 28 days (p <0.010). However, the results of this study showed that there was no increase in testosterone levels in the control group either before or after the placebo treatment (p> 0.05).Conclusion: The results of this study indicate that the administration of deer placenta orally for 28 days showed an increase in testosterone levels among young male Wistar rats.Â Latar Belakang: Laki-laki yang mengalami gejala-gejala hipogonad semakin dialami oleh usia yang semakin muda. Hal ini dikarenakan terjadi penurunan hormone testosterone. Upaya meningkatkan hormon testosteron pada laki-laki hipogonad kerap dilakukan untuk dapat mengembalikan fungsi seksual, libido, masa otot, kekuatan fisik, densitas tulang, dan perasaan senang. Penelitian ini bertujuan untuk mengetahui efek pemberian deer placenta secara oral dalam meningkatkan kadar hormon testosteron pada tikus Wistar (Rattus norvegicus) jantan muda.Metode: Penelitian eksperimental dilakukan dengan menggunakan pretest and posttest control group design yang melibatkan 18 ekor tikus putih Wistar jantan muda berusia 2,5-3 bulan dengan berat badan 150-180 gram. Tikus Wistar terbagi menjadi dua kelompok masing-masing berjumlah 9 ekor tikus dimana satu kelompok sebagai kelompok kontrol (pemberian plasebo selama 28 hari) dan kelompok perlakuan (pemberian deer placenta secara oral sebanyak 10,5 gram per hari selama 28 hari).Â Data yang diperoleh dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian menunjukkan terdapat peningkatan kadar testostesteron secara bermakna baik sebelum (3,018Â±0,282 ng/ml) maupun setelah (4,516Â±0,796 ng/ml) pemberian deer placenta secara oral sebanyak 10,5 gram selama 28 hari (p<0,010). Akan tetapi hasil penelitian ini menunjukkan tidak terdapat peningkatan kadar testosteron pada kelompok kontrol baik sebelum atau setelah perlakuan yang diberikan plasebo (p>0,05).Kesimpulan: Hasil penelitian ini menunjukkan bahwa pemberian deer placenta secara oral selama 28 hari menunjukkan terdapat peningkatan kadar testosteron pada tikus Wistar jantan muda.

Krim Ekstrak Bawang Hitam (Allium sativum Linn) Mencegah Peningkatan Ekspresi MMP-1 dan Penurunan Jumlah Kolagen Dermis Kulit Tikus (Rattus norvegicus) Wistar Jantan yang Dipapar Sinar UV-B Balqis Wasliati; Wimpie Pangkahila; A.A.G.P Wiraguna
Cermin Dunia Kedokteran Vol. 46 No. 8 (2019): Pediatri
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v46i8.420

Pendahuluan: Bawang hitam berasal dari bawang putih (Allium sativum Linn) melalui proses pemanasan, sehingga terjadi peningkatan kandungan antioksidan: fenolik, flavonoid (quarcetin), dan DPPH (IC-50). Tujuan penelitian ini adalah membuktikan krim ekstrak bawang hitam (Allium sativum Linn) mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis pada kulit tikus (Rattus norvegicus) Wistar jantan yang dipapar sinar UV-B. Metode: Penelitan ini adalah penelitian eksperimental post-test only control group design. Sampel penelitian ini terdiri dari 36 ekor tikus (Rattus norvegicus) Wistar jantan, dibagi menjadi 2 kelompok perlakuan secara acak. Tikus diberi bahan dasar krim dan dipapar sinar UV-B disebut kelompok kontrol, tikus diberi krim ekstrak bawang hitam 15% dan dipapar sinar UV-B disebut kelompok perlakuan. Total dosis UV-B 840 mJ/cm2 selama 4 minggu. Setelah perlakuan, tikus dieuthanasia dengan ketamine-xylazine lalu ekspresi MMP-1 diperiksa secara imunohistokimia dan jumlah kolagen dermis kulitnya menggunakan pewarnaan Pico-Sirius-Red. Hasil: Rerata ekspresi MMP-1 kelompok kontrol 28,58% dan kelompok perlakuan 8,60%; p<0,001 (uji t independent); terdapat perbedaan bermakna ekspresi MMP-1 antara kelompok kontrol dan kelompok perlakuan. Rerata jumlah kolagen kelompok kontrol 57,53% dan kelompok perlakuan 83,80%; p<0,001 (uji t independent); terdapat perbedaan bermakna jumlah kolagen antara kelompok kontrol dan kelompok perlakuan. Simpulan: Krim ekstrak bawang hitam (Allium sativum Linn) 15% mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis kulit tikus (Rattus norvegicus) Wistar jantan yang dipapar sinar UV-B. Aim: Black garlic was produced from garlic (Allium sativum Linn) through the heating process to increase the antioxidant content: phenolic, flavonoid (quarcetin), and DPPH (IC-50). The aim of this study is to prove that black garlic extract cream (Allium sativum Linn) application prevents enhancement of MMP-1 expression and reduction of dermal collagen in male Wistar rat (Rattus norvegicus) skin which has been exposed to UV-B light. Method: An experimental research using post-test only control group design. Total samples of 36 male Wistar rats (Rattus norvegicus) were divided into 2 groups. Control group were given cream and UV-B exposure (P0), treatment group were given 15% black garlic cream extract and UV-B exposure (P1). The total dose of UV-B was mJ/cm2 for 4 weeks. The rats were euthanized with ketamine-xylazine after treatment; MMP1 expression was examined using immunohistochemistry and dermal collagen using Pico-Sirius-Red staining. Result: MMP-1 expression of control group 28.58% and of treatment group 8.60% (independent t test, p<0.001). Mean of collagen in control group 57.53% and in treatment group 83.8% (independent t test, p<0.001). Conclusion: Black garlic extract cream 15% prevents the enhancement of MMP-1 expression and the reduction of dermal collagen in male Wistar rat (Rattus norvegicus) skin which has been exposed to UV-B light.

Addition of 5% Saccharide Isomerates in Moisturizing Formulation Increases Skin Hydration Higher than Regular Moisturizers Dian Andriani Ratna Dewi; Wimpie Pangkahila
Eduvest - Journal of Universal Studies Vol. 2 No. 8 (2022): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Dry skin is a problem for millions of people and often causes discomfort and even psychological stress. Increasing the water content in the stratum corneum can be done by applying moisturizer regularly and regularly because it will improve lipid levels and hydrate the epidermis. Saccharide isomers (SI) are one of the answers for the development of Glycobiology. The hypothesis of this study is that the addition of 5% SI in the moisturizing formulation further increases skin hydration and can maintain higher skin hydration even after discontinuation of use compared to ordinary moisturizers. The research subjects were 30 women aged 30-45 years who were not menopausal. Randomly divided into control group (15 people) and treatment group (15 people) by double blind. Moisturizer used for 2 weeks in a row, then discontinued use. Measurement of skin hydration was carried out 3 times a week during the use of moisturizer and after discontinuation. The non-invasive measuring instrument used is the Multi Skin Test Center® MC 750 made in Germany. Giving moisturizer in both groups resulted in an increase in skin hydration after 2 weeks of use (p<0.05). After discontinuation of the moisturizer, the four locations showed significant differences in skin hydration (p<0.05). From the results of this study, it can be concluded that the addition of 5% SI in the moisturizing formulation can increase skin hydration higher and can maintain higher skin hydration even after discontinuation of administration compared to ordinary moisturizers.

Peppermint Leaf Extract Cream Increased Transforming Growth Factor β (TGF-β) Expression and Collagen Amount In The Male Wistar Rat's Skin Exposed to UVB Made Ayu Mirah Wulandari; Anak Agung Gede Putra Wiraguna; Wimpie Pangkahila
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 13 No 3 (2023): Jurnal Ilmiah Permas: jurnal Ilmiah STIKES Kendal: Juli 2023
Publisher : Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32583/pskm.v13i3.1044

Peppermint leaves contain flavonoids, menthofuran, and other phenolic compounds that have antioxidant activity and are believed to inhibit collagen degradation in the skin. This study investigated the efficacy of a 5% peppermint leaf extract cream in increasing the transforming growth factor β (TGF-β) expression and collagen amount as indicators of collagen maintenance in male Wistar rats' skin exposed to UVB. Method A randomized post-test-only control group design was performed on male Wistar rats, local strains, aged 2-3 months, with body weights ranging from 180 to 200 grams. According to the sample calculation, 21 rats were randomly divided into three groups. The first group is the control group (without any treatment). The second group was treated with UVB without cream (UVB-only group), and the third group was treated with UVB + 5% peppermint leaf extract cream on exposed skin. After four weeks of treatment, the tissue samples were examined. The expression of TGF-β was evaluated by immunohistochemical examination, and the amount of collagen was examined by Picro-Sirius red staining. Comparative tests were performed on the measurement results of the three groups. The results showed a significant difference in the mean TGF-β expression and collagen amount among the three groups (p<0.001). The group exposed to UVB + 5% peppermint leaf extract cream had the highest mean TGF-β expression and collagen amount (77.41±5.79% and 82.57±2.18%, respectively), followed by the control group (63.17±12.22% and 74.05±7.15%) and the UVB-only group (50.54±11.10% and 65.65±5.56%). Administration of 5% peppermint leaf extract cream effectively increased TGF-β expression and collagen amount in the skin of male Wistar rats exposed to UVB compared to the control and the UVB-only groups.

The Correlation between TNF-α Serum Level and Andropause with Quality of Life on Elderly Trisna Yuliharti Tersinanda; RA Tuty Kuswardhani; Wimpie I Pangkahila
Indonesian Journal of Global Health Research Vol 2 No 3 (2020): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

As the population of elderly increases, the number of andropause also increases. Andropause is characterized by the decrease of testosteron level which is accompanied by hypogonadism symptoms. Many studies mentioned that decreased testosterone level will contribute to inflammation state, one of the cytokine is TNF-α, which then lead to many chronic inflammation disorders and influence the quality of life. Understanding the significant correlation between androgen and inflammation allows accurate consideration making in using androgen replacement therapy, therefore the quality of life will be improved significantly.This research was an analytical cross-sectional study. Subjects were recruited through random sampling at Denpasar in 2019. TNF-α serum level was measured by human immunoassay method. Andropause was measured by ADAM questionnaire and confirmed by total decreased testosterone serum level. Quality of life was measured by WHOQOL-BREF questionnaire which was then continued by bivariate and multivariate analysis to control the confounding variables. It was considered statistically significant if P value is more than 0.05. There were 60 elderly involved as research subjects consisting of 43 andropause cases and 17 non andropause cases. There was significant correlation between TNF-α serum and quality of life. This study also found that there was significant difference in quality of life between andropause and non andropause group. This study shows that there was significant correlation between TNF-α serum level and andropause with quality of life in elderly.

Krim Ekstrak Bawang Hitam (Allium sativum Linn) Mencegah Peningkatan Ekspresi MMP-1 dan Penurunan Jumlah Kolagen Dermis Kulit Tikus (Rattus norvegicus) Wistar Jantan yang Dipapar Sinar UV-B Balqis Wasliati; Wimpie Pangkahila; A.A.G.P Wiraguna
Cermin Dunia Kedokteran Vol 46 No 8 (2019): Pediatri
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v46i8.420

Pendahuluan: Bawang hitam berasal dari bawang putih (Allium sativum Linn) melalui proses pemanasan, sehingga terjadi peningkatan kandungan antioksidan: fenolik, flavonoid (quarcetin), dan DPPH (IC-50). Tujuan penelitian ini adalah membuktikan krim ekstrak bawang hitam (Allium sativum Linn) mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis pada kulit tikus (Rattus norvegicus) Wistar jantan yang dipapar sinar UV-B. Metode: Penelitan ini adalah penelitian eksperimental post-test only control group design. Sampel penelitian ini terdiri dari 36 ekor tikus (Rattus norvegicus) Wistar jantan, dibagi menjadi 2 kelompok perlakuan secara acak. Tikus diberi bahan dasar krim dan dipapar sinar UV-B disebut kelompok kontrol, tikus diberi krim ekstrak bawang hitam 15% dan dipapar sinar UV-B disebut kelompok perlakuan. Total dosis UV-B 840 mJ/cm2 selama 4 minggu. Setelah perlakuan, tikus dieuthanasia dengan ketamine-xylazine lalu ekspresi MMP-1 diperiksa secara imunohistokimia dan jumlah kolagen dermis kulitnya menggunakan pewarnaan Pico-Sirius-Red. Hasil: Rerata ekspresi MMP-1 kelompok kontrol 28,58% dan kelompok perlakuan 8,60%; p<0,001 (uji t independent); terdapat perbedaan bermakna ekspresi MMP-1 antara kelompok kontrol dan kelompok perlakuan. Rerata jumlah kolagen kelompok kontrol 57,53% dan kelompok perlakuan 83,80%; p<0,001 (uji t independent); terdapat perbedaan bermakna jumlah kolagen antara kelompok kontrol dan kelompok perlakuan. Simpulan: Krim ekstrak bawang hitam (Allium sativum Linn) 15% mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis kulit tikus (Rattus norvegicus) Wistar jantan yang dipapar sinar UV-B. Aim: Black garlic was produced from garlic (Allium sativum Linn) through the heating process to increase the antioxidant content: phenolic, flavonoid (quarcetin), and DPPH (IC-50). The aim of this study is to prove that black garlic extract cream (Allium sativum Linn) application prevents enhancement of MMP-1 expression and reduction of dermal collagen in male Wistar rat (Rattus norvegicus) skin which has been exposed to UV-B light. Method: An experimental research using post-test only control group design. Total samples of 36 male Wistar rats (Rattus norvegicus) were divided into 2 groups. Control group were given cream and UV-B exposure (P0), treatment group were given 15% black garlic cream extract and UV-B exposure (P1). The total dose of UV-B was mJ/cm2 for 4 weeks. The rats were euthanized with ketamine-xylazine after treatment; MMP1 expression was examined using immunohistochemistry and dermal collagen using Pico-Sirius-Red staining. Result: MMP-1 expression of control group 28.58% and of treatment group 8.60% (independent t test, p<0.001). Mean of collagen in control group 57.53% and in treatment group 83.8% (independent t test, p<0.001). Conclusion: Black garlic extract cream 15% prevents the enhancement of MMP-1 expression and the reduction of dermal collagen in male Wistar rat (Rattus norvegicus) skin which has been exposed to UV-B light.

Administration of Corosolic Acid Decreased MDA, HMG-CoA, Through Increased Leptin and GLP-1 Levels in Obese Male Rats Mochtar, Fransiska; Pangkahila, Wimpie I.; Dewi, I Gusti Ayu Sri Mahendra; Saraswati, Made Ratna; Azizah, Norma Nur Azizah
Asian Journal of Social and Humanities Vol. 2 No. 03 (2023): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v2i03.204

Obesity is a condition where excessive fat is accumulated, which poses a health risk. Several treatments for obesity have been carried out, ranging from lifestyle adjustments to medication to surgery. Corrosolic acid (CA) is a natural compound that has the potential to act as an obesity agent through many mechanisms, including through reactive oxygen species (ROS). This study aims to prove that CA can reduce MDA levels and HMG-CoA reductase levels and increase leptin levels and GLP-1 levels in male Wistar rats with obesity. Wistar obese rats were orally treated with CA compound induction at a dose of 10 mg/Kg BW. MDA, HMG-CoA reductase, leptin and GLP-1 levels were examined using serum and plasma from mice in both groups of mice before and after treatment, using the ELISA method. CA to the treatment group can reduce the body weight of rat MDA levels significantly at p<0.0001, HMG-CoA reductase levels significantly at p<0.0001 and increase leptin and GLP-1 levels significantly at p<0.0001. CA's mechanism for treating obesity is through the ROS mechanism because CA has antioxidant levels that can capture free radicals in the body.CA has been proven to be an alternative drug in treating obesity by reducing body weight, MDA, and HMG-CoA levels and increasing leptin and GLP-1 levels in obese Wistar rats.

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