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Journal : Menara Perkebunan

Respons bibit kelapa sawit (Elaeis guineensis Jacq.) terhadap pemberian fungi mikoriza arbuskular dan cekaman air [Oil palm (Elaeis guineensis Jacq.) seedling response to application of arbuscular mycorrhiza fungi and water stress] Maria Viva RINI; Usnaqul EFRIYANI
E-Journal Menara Perkebunan Vol 84, No 2 (2016): Desember 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.988 KB) | DOI: 10.22302/iribb.jur.mp.v84i2.225

Abstract

 The objective of this study was to determine the response of oil palm seedling to the application of arbuscular mycorrhiza fungi (AMF) subjected to water stress.  The treatment design used was a factorial design 2 x 5 arranged in completely randomized design.  The first factor was application of AMF i.e. without AMF (control) and inoculation with AMF (mixture of Glomus sp., Entropospora sp., and Gigaspora sp.).  The second factor was water stress i.e. no watering for 0, 7, 14, 21, and 28 days at the end of experiment.  Data obtained were subjected to Bartlett test to examine the treatment variance, and Tukey tests to examine additiveness of the data, and continued by analysis of variance. The results showed that application of AMF increased oil palm seedling growth through the increase in seedling height, number of leave, shoot fresh and dry weight, and percent of root infection.  Duration of water stress significantly affected oil palm seedling growth by decreasing plant height, number of leave, shot and root fresh weight, root dry weight. However, at any level of water stress, the AMF inoculated seedling had better growth compared to those control palms.[Key words: oil palm seedling, arbuscular mycorrhiza fungi (AMF), water stress] AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh pemberian fungi mikoriza arbuskular (FMA) terhadap respons pertumbuhan bibit kelapa sawit yang mengalami cekaman air. Perlakuan disusun secara faktorial 2x5 dalam rancangan kelompok teracak sempurna (RKTS) dengan lima ulangan.  Faktor pertama adalah aplikasi FMA, yaitu kontrol (tanpa FMA) dan diberi FMA (campuran Glomus sp., Entropospora sp., dan Gigaspora sp.).  Faktor kedua adalah lamanya cekaman air, yaitu 0, 7, 14, 21, dan 28 hari tanpa disiram sebelum penelitian dihentikan.  Data yang diperoleh diuji kesamaan ragam antar perlakuannya dengan Uji Bartlett dan kemenambahan modelnya dengan Uji Tukey.  Jika asumsi terpenuhi, ragam homogen dan data bersifat menambah, maka data dianalisis ragam. Hasil penelitian menunjukkan bahwa aplikasi FMA meningkatkan pertumbuhan bibit kelapa sawit melalui peningkatan tinggi tanaman, jumlah daun, bobot basah tajuk, bobot kering tajuk, dan persen infeksi akar. Lamanya cekaman air menurunkan pertumbuhan bibit kelapa sawit melalui penurunan tinggi tanaman, jumlah daun, jumlah akar primer, bobot basah tajuk, bobot basah akar, bobot kering tajuk, dan bobot kering akar, akan tetapi pada setiap  taraf cekaman air, pertumbuhan bibit yang ber-FMA lebih tinggi dibandingkan dengan tanpa FMA.[Kata kunci: bibit kelapa sawit, fungi mikoriza arbuskular (FMA), cekaman air]
Selection of four types arbuscular mycorrhizal fungi in oil palm seedling planted in histosol soil Maria Viva RINI; Radix SUHARJO; Lestari WIBOWO; David IRVANTO; Adhy ARIYANTO
E-Journal Menara Perkebunan Vol 89, No 1 (2021): April, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.406

Abstract

Arbuscular mycorrhizal fungi (AMF) are beneficial microbes for plants, especially in the absorption of nutrients and water from the soil. Some factors that influence the effectiveness of this fungus are the suitability of the AMF species with their host plants and abiotic condition such as soil characteristics. Therefore, this study aims to determine the type of AMF that produced the best growth and nutrient uptake in oil palm seedlings planting in histosol soil. This study used a single factor treatment design consisting of 9 AMF treatments, namely control without AMF (T1), given Glomus sp. (T2), Gigaspora sp. (T3), Entrophospora sp. (T4), Acaulospora sp. (T5), a mixture of Glomus sp. and Gigaspora sp. (T6), a mixture of Glomus sp. and Entrophospora sp. (T7), a mixture of Gigaspora sp. and Entrophospora sp. (T8), and a mixture of Glomus sp., Gigaspora sp., Entrophospora sp., and Acaulospora sp. (T9). Each treatment was repeated five times. The experimental design used was a randomized block design. In the AMF treatment, 500 spores per seedling were given when the seedlings were transplanted from prenursery to the main nursery. The seedlings were kept in the prenursery for 3.5 months and in the main nursery for nine months. The results showed that the type of mycorrhizal that consistently produced better seedling growth and nutrients uptake compared to the control were Glomus sp. (T2) and a mixture of Gigaspora sp. and Entrophospora sp. (T8), which supported by data on plant height, number of leaves, shoot dry weight, and total nutrient uptake of N, P, K, Ca, Mg, and B.
Asosiasi Glomus sp. dan Gigaspora margarita pada bibit Aquilaria malaccensis Endah SUSILOWATI; Melya RINIARTI; Maria Viva RINI
E-Journal Menara Perkebunan Vol 87, No 2 (2019): OKTOBER, 2019
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.329 KB) | DOI: 10.22302/iribb.jur.mp.v87i2.342

Abstract

AbstractAgarwood (Aquilaria malaccensis) is one of the non-timber forest products that has high economic value. However, this plant grows very slowly.  Therefore, seed technology input is needed, such as addition of arbuscular mycorrhizal fungi (AMF). The objectives of this research were to study the effect of AMF inoculum i.e. Glomus sp., Gigaspora margarita and mixture of both species on agarwood growth and to determine the best AMF type for agarwood seedlings growth. The experimental research design used was a completely randomized design with three treatments, namely Glomus sp. (G), G. margarita (Gi) and a mixture of Glomus sp. and G. margarita (GGi) and without AMF inoculation (K) with each treatment repeated 8 times. The number of AMF spores used was ± 300 spores/seedling, and applied at the time of transplanting from germination tray to the polybag. Data were processed using analysis of variance and least significant difference test (LSD). The results showed there was AMF colonization in agarwood roots after 12 weeks of inoculation with the highest percentage at 20.50% on G. margarita treatment. Overall, AMF improved the growth of agarwood seedlings. The best seedlings growth was in the treatment of mixed AMF (GGi). Increased growth was found in plant height (14.68 cm), seedling diameter (2.16 mm), leaf area (119.30 cm2), root volume (1.15 mL), total dry weight (0.83 g), and shoot root ratio (4.99).[Keywords: agarwood, A. malaccensis,AMF, G. margarita,Glomussp.]AbstrakGaharu (Aquilariamalaccensis) adalah salah satu hasil hutan bukan kayu yang memiliki nilai ekonomis tinggi. Namun tanaman ini memiliki pertumbuhan yang lambat, sehingga diperlukan input teknologi pembibitan berupa penambahan fungi mikoriza arbuskular (FMA). Penelitian bertujuan untuk mengetahui pengaruh pemberian FMA tunggal Glomussp., Gigaspora margaritadan campuran keduanya (Glomussp. dan G. margarita)terhadap pertumbuhan gaharu serta menentukan isolatyang menghasilkan pertumbuhan bibit gaharu yang lebih baik.  Rancangan percobaan yang digunakan adalah rancangan acak lengkap untuk menguji tiga perlakuan yaitu Glomussp. (G), G. margarita (Gi) dan campuran Glomus sp. dan G. margarita(GGi) serta satu kontrol (K) dengan  masing – masing perlakuan diulang 8 kali. Inokulum FMA yang digunakan memiliki kepadatan ±300 spora/bibit dan diinokulasikan saat bibit dipindahkan dari persemaian ke polybag. Data diolah menggunakan analisis varians dan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa ditemukan adanya kolonisasi FMA dalam akar gaharu setelah 12 minggu inokulasi dengan persentase kolonisasi tertinggi 20,50% oleh G. margarita.  Secara keseluruhan, pemberian FMA mampu meningkatkan pertumbuhan bibit gaharu. Pertumbuhan terbaik terjadi pada perlakuan FMA campuran (GGi).  Peningkatan pertumbuhan secara nyata dapat dilihat pada tinggi tanaman (14,68 cm), diameter bibit (2,16 mm), luas daun (119,30 cm2), volume akar (1,15 mL), bobot kering total (0,83 g) dan nisbah tajuk akar (4,99).[Kata kunci: A. malaccensis, FMA, gaharu, G. margarita, Glomus sp.]
Respons bibit kelapa sawit (Elaeis guineensis Jacq.) terhadap pemberian fungi mikoriza arbuskular dan cekaman air [Oil palm (Elaeis guineensis Jacq.) seedling response to application of arbuscular mycorrhiza fungi and water stress] Maria Viva RINI; Usnaqul EFRIYANI
Menara Perkebunan Vol. 84 No. 2 (2016): 84 (2), 2016
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v84i2.225

Abstract

 The objective of this study was to determine the response of oil palm seedling to the application of arbuscular mycorrhiza fungi (AMF) subjected to water stress.  The treatment design used was a factorial design 2 x 5 arranged in completely randomized design.  The first factor was application of AMF i.e. without AMF (control) and inoculation with AMF (mixture of Glomus sp., Entropospora sp., and Gigaspora sp.).  The second factor was water stress i.e. no watering for 0, 7, 14, 21, and 28 days at the end of experiment.  Data obtained were subjected to Bartlett test to examine the treatment variance, and Tukey tests to examine additiveness of the data, and continued by analysis of variance. The results showed that application of AMF increased oil palm seedling growth through the increase in seedling height, number of leave, shoot fresh and dry weight, and percent of root infection.  Duration of water stress significantly affected oil palm seedling growth by decreasing plant height, number of leave, shot and root fresh weight, root dry weight. However, at any level of water stress, the AMF inoculated seedling had better growth compared to those control palms.[Key words: oil palm seedling, arbuscular mycorrhiza fungi (AMF), water stress] AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh pemberian fungi mikoriza arbuskular (FMA) terhadap respons pertumbuhan bibit kelapa sawit yang mengalami cekaman air. Perlakuan disusun secara faktorial 2x5 dalam rancangan kelompok teracak sempurna (RKTS) dengan lima ulangan.  Faktor pertama adalah aplikasi FMA, yaitu kontrol (tanpa FMA) dan diberi FMA (campuran Glomus sp., Entropospora sp., dan Gigaspora sp.).  Faktor kedua adalah lamanya cekaman air, yaitu 0, 7, 14, 21, dan 28 hari tanpa disiram sebelum penelitian dihentikan.  Data yang diperoleh diuji kesamaan ragam antar perlakuannya dengan Uji Bartlett dan kemenambahan modelnya dengan Uji Tukey.  Jika asumsi terpenuhi, ragam homogen dan data bersifat menambah, maka data dianalisis ragam. Hasil penelitian menunjukkan bahwa aplikasi FMA meningkatkan pertumbuhan bibit kelapa sawit melalui peningkatan tinggi tanaman, jumlah daun, bobot basah tajuk, bobot kering tajuk, dan persen infeksi akar. Lamanya cekaman air menurunkan pertumbuhan bibit kelapa sawit melalui penurunan tinggi tanaman, jumlah daun, jumlah akar primer, bobot basah tajuk, bobot basah akar, bobot kering tajuk, dan bobot kering akar, akan tetapi pada setiap  taraf cekaman air, pertumbuhan bibit yang ber-FMA lebih tinggi dibandingkan dengan tanpa FMA.[Kata kunci: bibit kelapa sawit, fungi mikoriza arbuskular (FMA), cekaman air]
Asosiasi Glomus sp. dan Gigaspora margarita pada bibit Aquilaria malaccensis Endah SUSILOWATI; Melya RINIARTI; Maria Viva RINI
Menara Perkebunan Vol. 87 No. 2 (2019): 87 (2), 2019
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v87i2.342

Abstract

AbstractAgarwood (Aquilaria malaccensis) is one of the non-timber forest products that has high economic value. However, this plant grows very slowly.  Therefore, seed technology input is needed, such as addition of arbuscular mycorrhizal fungi (AMF). The objectives of this research were to study the effect of AMF inoculum i.e. Glomus sp., Gigaspora margarita and mixture of both species on agarwood growth and to determine the best AMF type for agarwood seedlings growth. The experimental research design used was a completely randomized design with three treatments, namely Glomus sp. (G), G. margarita (Gi) and a mixture of Glomus sp. and G. margarita (GGi) and without AMF inoculation (K) with each treatment repeated 8 times. The number of AMF spores used was ± 300 spores/seedling, and applied at the time of transplanting from germination tray to the polybag. Data were processed using analysis of variance and least significant difference test (LSD). The results showed there was AMF colonization in agarwood roots after 12 weeks of inoculation with the highest percentage at 20.50% on G. margarita treatment. Overall, AMF improved the growth of agarwood seedlings. The best seedlings growth was in the treatment of mixed AMF (GGi). Increased growth was found in plant height (14.68 cm), seedling diameter (2.16 mm), leaf area (119.30 cm2), root volume (1.15 mL), total dry weight (0.83 g), and shoot root ratio (4.99).[Keywords: agarwood, A. malaccensis,AMF, G. margarita,Glomussp.]AbstrakGaharu (Aquilariamalaccensis) adalah salah satu hasil hutan bukan kayu yang memiliki nilai ekonomis tinggi. Namun tanaman ini memiliki pertumbuhan yang lambat, sehingga diperlukan input teknologi pembibitan berupa penambahan fungi mikoriza arbuskular (FMA). Penelitian bertujuan untuk mengetahui pengaruh pemberian FMA tunggal Glomussp., Gigaspora margaritadan campuran keduanya (Glomussp. dan G. margarita)terhadap pertumbuhan gaharu serta menentukan isolatyang menghasilkan pertumbuhan bibit gaharu yang lebih baik.  Rancangan percobaan yang digunakan adalah rancangan acak lengkap untuk menguji tiga perlakuan yaitu Glomussp. (G), G. margarita (Gi) dan campuran Glomus sp. dan G. margarita(GGi) serta satu kontrol (K) dengan  masing – masing perlakuan diulang 8 kali. Inokulum FMA yang digunakan memiliki kepadatan ±300 spora/bibit dan diinokulasikan saat bibit dipindahkan dari persemaian ke polybag. Data diolah menggunakan analisis varians dan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa ditemukan adanya kolonisasi FMA dalam akar gaharu setelah 12 minggu inokulasi dengan persentase kolonisasi tertinggi 20,50% oleh G. margarita.  Secara keseluruhan, pemberian FMA mampu meningkatkan pertumbuhan bibit gaharu. Pertumbuhan terbaik terjadi pada perlakuan FMA campuran (GGi).  Peningkatan pertumbuhan secara nyata dapat dilihat pada tinggi tanaman (14,68 cm), diameter bibit (2,16 mm), luas daun (119,30 cm2), volume akar (1,15 mL), bobot kering total (0,83 g) dan nisbah tajuk akar (4,99).[Kata kunci: A. malaccensis, FMA, gaharu, G. margarita, Glomus sp.]
Selection of four types arbuscular mycorrhizal fungi in oil palm seedling planted in histosol soil Maria Viva RINI; Radix SUHARJO; Lestari WIBOWO; David IRVANTO; Adhy ARIYANTO
Menara Perkebunan Vol. 89 No. 1 (2021): 89 (1), 2021
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.406

Abstract

Arbuscular mycorrhizal fungi (AMF) are beneficial microbes for plants, especially in the absorption of nutrients and water from the soil. Some factors that influence the effectiveness of this fungus are the suitability of the AMF species with their host plants and abiotic condition such as soil characteristics. Therefore, this study aims to determine the type of AMF that produced the best growth and nutrient uptake in oil palm seedlings planting in histosol soil. This study used a single factor treatment design consisting of 9 AMF treatments, namely control without AMF (T1), given Glomus sp. (T2), Gigaspora sp. (T3), Entrophospora sp. (T4), Acaulospora sp. (T5), a mixture of Glomus sp. and Gigaspora sp. (T6), a mixture of Glomus sp. and Entrophospora sp. (T7), a mixture of Gigaspora sp. and Entrophospora sp. (T8), and a mixture of Glomus sp., Gigaspora sp., Entrophospora sp., and Acaulospora sp. (T9). Each treatment was repeated five times. The experimental design used was a randomized block design. In the AMF treatment, 500 spores per seedling were given when the seedlings were transplanted from prenursery to the main nursery. The seedlings were kept in the prenursery for 3.5 months and in the main nursery for nine months. The results showed that the type of mycorrhizal that consistently produced better seedling growth and nutrients uptake compared to the control were Glomus sp. (T2) and a mixture of Gigaspora sp. and Entrophospora sp. (T8), which supported by data on plant height, number of leaves, shoot dry weight, and total nutrient uptake of N, P, K, Ca, Mg, and B.
Co-Authors Adhy ARIYANTO Agus Karyanto Agus Karyanto Ainin Niswati Ananda Y. Rahmayanti Arif, M. A. Syamsul Aulia, Fathia Nur Azzahra, Salwa Catur Putra Satgada Catur Putra Satgada, Catur Putra Citra Bara Kurniastuty Dad Resiworo J. Sembodo David IRVANTO Decha Bagus Saputra Dermiyati Dermiyati Eldineri Zulkarnain Eldineri Zulkarnain, Eldineri Endah SUSILOWATI Fitri Yelli Fluenty Dwitama Hanum Riajeng Amalia Hanum Riajeng Amalia, Hanum Riajeng Hidayat Pujisiswanto Hidayat Saputra Hindun Nur Haqiqie Husna Fii Karisma Jannah Husna Husna Ina Febria Ginting Inggar Damayanti Jamalam Lumbanraja Jamalam Lumbanraja Krisnarini, Krisnarini Kus Hendarto Kusuma Oka Pertiwi Kuswanta Futas Hidayat Kuswanta Futas Hidayat Kuswanta Futas Hidayat Kuswanta Futas Hidayat Lestari Wibowo Listyowati, Mastutik Sri Lita Andriyyani M. A. Syamsul Arif M. A. Syamsul Arif Mas Achmad Syamsul Arif Maulana Malik Melya Riniarti Minarsih Minarsih Muhamad Gary Ranchiano Novalim Purlasyanko Novri Dwi Damayanti Novri Dwi Damayanti Palasta, Rio Paul Benyamin Timotiwu Puput Azizah Raden Ajeng Diana Widyastuti Radix Suharjo Ramadian Budi Santoso Ramires, Ryano Retta Ramadhina Rias Retta Ramadhina Rias Rio Palasta Rugayah Rusdi Evizal Sanjaya, Purba Santoso, Ramadian Budi Sari, Oktafia Sarno Sarno Septiana, Liska Mutiara Sholihah, Fairuz Nabila Silfi Indrasari Sitio, Selly Novita Sari Sri Yusnaini Sri Yusnaini Sri Yusnaini Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Syaifudin Nur Hasan Tegar Rafshodi Awang Tegar Rafshodi Awang, Tegar Rafshodi Titiek Nur Aeny Usnaqul EFRIYANI Vida Rozalinda Wiwik Agustina Wiwik Agustina, Wiwik Yunita Siwi Palupi