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Pagarra, Halifah
FMIPA, Universitas Negeri Makassar
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Optimisasi Enzim Protease oleh Bakteri Endofit dari Akar Tumbuhan Kawasan Ekosistem Karstdengan Response Surface Methodology Halifah Pagarra; Hartati Hartati; Rachmawaty Rachmawaty
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 10 No. 2 (2021): Volume 10 Nomor 2 (September 2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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This study aims to determine the activity of protease enzyme production from bacterial isolate B isolated from the roots of karst plants in various incubation times. The bacterial isolate used was bacterial isolate B, a thermophilic proteolytic bacterium isolated from the Karst Ecosystem in Pangkajene Regency, South Sulawesi Province. Testing of protease activity was carried out using the Lowry method. Highest crude protein. Protein content of all treatments was obtained at 48 hours of incubation for Bacterial Isolate B (5.756 mg/ml).The results showed that Bacterial Isolate B showed optimal production time at 48 hours with enzyme activity 0.393 Unit/ml. It can be concluded that Bacterial Isolate B is a proteolytic bacterium characterized by the formation ofa clear zone around the bacterial colonies in SMA. The production of the protease enzyme obtained by the RSM (CCD) method was optimum at 0.393 U/ml at 0.60% glucose concentration, 0.50% yeast extract and 1.00% skim milk.
Pembentukan Gaharu Pada Pohon Aquilaria malaccensis Lamk., Menggunakan Inokulum Fusariumsp. Rachmaway Rachmaway; Asraful Ashar; Alimuddin Ali; Halifah Pagarra; Siti Fatmah Hiola
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 10 No. 2 (2021): Volume 10 Nomor 2 (September 2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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This study aims to determine the effect of artificial nutrient formulations and inoculum Fusarium sp., to produce agarwood in Aquilaria malaccensis Lamk., using the infusion method. This study used 3 types of treatment, namely, P1 inoculum formulation (artificial nutrition + Fusarium sp.), P2 formulation (artificial nutrition + Fusarium sp. fermentation) and P3 formulation (artificial nutrition + Fusarium sp. + fermented A. malaccensis Lamk sawdust) and formulation P4 was Fusarium sp. inoculum along with organic nutrients as control. The study was conducted on farmer's agroforestry land in Soppeng Regency, BaraE Village, South Sulawesi, using the induction infusion method in which 12 holes were drilled, 10 cm apart from each hole in a spiral pattern. The depth of each hole is 1/3 the diameter of the rod. Infusion was given after 3 months of inoculation with observation parameters such as area of infection, color change and aroma level. The results showed that the P2 formulation formed the area of agarwood infection with an average of 10.06 cm2. The organoleptic result for color change which showed a score of 3 out of 6 panelists was the P3 formulation, while the aroma level showed a score of 3 (very fragrant) out of 7 panelists, namely the P4 formulation. The results of this study concluded that the P3 formulation has the potential to provide better agarwood formation, the P4 formulation for the level of aroma and the extent of infection by the P2 formulation
Differences of Ethanol Extract and Ethyl Acetate of Pepper Leaf (Piper Nigrum L) Against Anti Microbial Activity Hartati; Halifah Pagarra
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 7 No. 1 (2018): Volume 7 Nomor 1 (Maret 2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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The aim of this research was to know the difference of ethanol extract and extract of ethyl acetate of pepper leaf (Piper nigrum L) to antimicrobial activity of Staphylococcua aureus, Escherichia coli and Candida albicans. The maceration method has been used to extract the pepper leaf by using two solvents ie 70% ethanol and ethyl acetate. The extract results were then tested for antimicrobial activity. The result of pepper ethanol extract obtainedby the higher yield was 24,64% compared with ethyl acetate extract 9,18%. Leaf pepper ethanol extract had higher antimicrobial activity than pepper acetate leaves extract against Staphylococcua aureus, and Escherichia coli but did not inhibit Candida albicans fungi at concentration of 10%
Identifikasi Senyawa Kulit Buah Kakao Sulawesi 2(Theobroma cacaoL.) Menggunakan Etanol dan Aseton Rachmawaty Rachmawaty; Halifah Pagarra; Nurul Anisa
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 11 No. 1 (2022): Volume 11 Nomor 1 (Maret 2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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This research was to determine the differences composition of chemical compounds the ethanol and acetone extract from cocoa pod husks based on qualitativephytochemical analysis and gas chromatography-mass spectrometry (GC-MS) analysis.Cocoa pod husk extraction was carried out by maceration method using 70% ethanol and acetone:water (7:3) as solvents. The yield results obtained showed that the cocoa pod husk extract that extracted by acetone had a higher yield than the ethanol solvent. In the qualitative phytochemical test using color reagents was found that ethanolic extract of cocoa pod husks contained flavonoids and phenols, while the acetone extract contain tannins, flavonoids, and phenols. The GC-MS test of ethanol extract of cocoa pod husks had identified 5 main compounds that classified to fatty acid and lactone compounds, while acetone extracthad also identified 5 main compounds that classified to glycerol, ester, disaccharide, phthalic ester, and ascorbic acid compounds.
Analisis Fitokimia dan Pengaruh Ekstrak Etanol Daun Kayu Jawa (Lannea coromandelica) terhadap Pertumbuhan Bakteri Staphylococcus aureus Halifah Pagarra; Sahribulan Sahribulan
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 11 No. 2 (2022): Volume 11 Nomor 2 (September 2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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Plants in Indonesia are very diverse and many of them are plants that can be used by the community as medicinal plants. One of them is the Javanese wood plant. Java wood is known to contain secondary metabolites such as alcohol, steroids, triterpenoids, phenolics, flavonoids, tannins, and saponins. This compound is a group of bioactive compounds that produce antioxidant, anti-inflammatory, antibacterial, anticancer and immunomodulatory activities. In this study, phytochemical testing of the compound content of the ethanolic extract of Javanese wood leaves was carried out and then looked at its effect on the growth of Staphylococcus aureus bacteria. The method used is to test the phytochemical content of alkaloid compounds, tannins, flavonoids and saponins. Observation of the effect of ethanolic extract of Javanese wood leaves on the growth of Staphylococcus aureus bacteria was carried out by testing the antibacterial activity of 96% ethanol extract of Javanese leaves with concentrations of 5%, 10%, 15%, 20%, 40%, 60%, and 80 respectively. %. The results showed that 96% ethanol extract of Javanese wood leaves contained alkaloids with Dragendorff reagents, tannins, flavonoids, and saponins. In testing the antibacterial activity of the 96% ethanol extract of Javanese wood leaves at the specified concentration, there was an inhibition zone formed, namely at a concentration of 5% at 9.4 mm, 10% at 11.6 mm, 15% at 14.4 mm, 20 % is 11.5 mm, 40% is 15.6 mm, 60% is 18.7 mm, and 80% is 18.8 mm.
Kerusakan Morfologis Paru-Paru Mencit Akibat Pemberian Asam Kuat Dengan Konsentrasi Berbeda A. Mu'nisa A. Mu'nisa; Rachmawaty Rachmawaty; Halifah Pagarra; Hilda Karim; Theodorus K. Tokan
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 11 No. 2 (2022): Volume 11 Nomor 2 (September 2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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This study aims to determine the damage to lung morphology in mice (Mus musculus) by administering various strong acids by inhalation. The string acids used in this study are HCl 30%, H2SO4 50%, and HNO3 50%). The subjects of the study were 2-month old mice which were divided into 4 treatment groups consisting of 2 mice per group. There are 4 gorups of mice; a control group, a treatment group with 37% hydrochloric acid (HCl), a group with with sulfuric acid (H2SO4) 50% treatment, and a group with nitric acid (HNO3) 50% treatment. Strong acids are given simultaneously with standard feed and drinking water ad libitium. Tthe mice (Mus musculus) were euthanized 10 days post treatment and surgery was performed to observe lung morphology consisting of color, weight, and shape. The study showed that the administration of strong acid damaged the lung morphology of mice and the agent causing most severe damage of lungs was the 50% nitric acid (HNO3).
Effect Of Blended Learning On Independence And Learning Outcomes Of Grade Xi Learners Khusnul Khatimah; Adnan; Halifah Pagarra
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 13 No. 2 (2024): Volume 13 Nomor 2 (September 2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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Research was carried out with the aim of finding out blended learning on the independence and learning outcomes of class XI MAN 1 Makassar City students. A posttest only control design was used in this research, which is a type of quasiexperimental research. Entire class of XI MAN 1 Makassar City, consisting of 12 classes, was used as the population, then selected by random sampling to obtain 4 classes as samples consisting of 3 experimental classes and 1 control class. Implementation of learning in experimental group used an ICT-based MPBK model with blended learning approach, while in the control group used direct instruction learning model with teacher-based learning approach. Data collection on learning independence is non-test instrument in the form of questionnaire and data collection on learning outcomes is test instrument in form of objective and subjective questions. Data results were then analyzed using descriptive statistics followed inferential statistics. Results of the descriptive analysis showed that the experimental group had better independence and learning outcomes than tcontrol group. Results of the inferential analysis used normality tests, homogeneity, hypothesis testing with multivariate analysis of variance tests and further Tukey HSD tests. From hypothesis testing it was found that the significance value was 0.000 < 0.05, so there was influence of blended learning on independence and learning outcomes of class XI MAN 1 Makassar City students. Results of further tests with Tukey HSD for independence and learning outcomes showed that experimental and control groups were in different subset columns so that there were significant differences between groups, which means that statistically blended learning has influence on students' independence and learning outcomes.
Antibacterial Activity of Pegagan (Centella asiatica) Leaf Extract and Fractions Against Escherichia coli and Staphylococcus aureus Halifah Pagarra; Erlin Salino; Hartati
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 13 No. 2 (2024): Volume 13 Nomor 2 (September 2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

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Pegagan atau yang dikenal dengan nama Pegagan merupakan tanaman perdu yang mengandung zat gizi dan senyawa aktif yang baik bagi tubuh, karena memiliki khasiat sebagai antibakteri, antivirus, antihipertensi, diuretik dan antiinflamasi. Tanaman ini merupakan sumber senyawa fenolik, flavonoid, triterpenoid, alkaloid dan tanin. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri daun pegagan. Daun pegagan diekstraksi dengan pelarut etanol 70%, kemudian difraksinasi dengan pelarut n-heksana, etil asetat dan metanol. Evaluasi aktivitas antibakteri menggunakan metode difusi cakram pada konsentrasi 5%, 10% dan 15% terhadap Escherichia coli dan Staphylococcus aureus dengan mengukur zona bening yang terbentuk di sekitar kertas cakram. Hasil penelitian menunjukkan bahwa ekstrak etanol 70%, fraksi etil asetat dan fraksi metanol:air memiliki aktivitas antibakteri terhadap kedua bakteri tersebut, sedangkan fraksi n-heksana tidak memiliki aktivitas antibakteri terhadap kedua bakteri tersebut. Ekstrak etanol dengan konsentrasi 5% menunjukkan aktivitas paling efektif dalam menghambat Escherichia coli dengan zona hambat 13,67 ± 0,428 mm dan fraksi metanol:air dengan konsentrasi 5% terhadap Staphylococcus aureus dengan zona hambat 9,35 ± 0,17 mm.
PENGEMBANGAN NANOEMULSI EKSTRAK DAUN KELOR (MORINGA OLEIFERA)SEBAGAI ANTIOKSIDAN,ANTIBAKTERIDAN ANTILUKA Muh. Akbar Hidayat; Hartati Hartati; Halifah Pagarra; Sahribulan Sahribulan
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 14 No. 01 (2025): Volume 14 Nomor 1 (Maret 2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/wgvnbx92

Abstract

This study aims to determine the effect of the concentration of nanoemulsion of moringa leaf extract (Moringa Oleifera Lamk.) on bacterial growth, antioxidant activity and wound healing potential in white rats (Rattus norvegicus). The research methods include moringa extraction, making nanoemulsions at moringa leaf extract concentrations of 75, 100 and 125 milligrams. Characterization of nanoemulsion measurements using Particle Size Analyzer (PSA), testing antibacterial activity by measuring the diameter ofthe inhibition zone (mm), testing antioxidants with DPPH (1,1 diphenyl-2-picrylhydrazyl) and measuring wound healing activity by determining the percentage of wound closure in rats. The study used a Completely Randomized Design (CRD) and Analysis of Variant (ANOVA) test followed by the Tukey test. Antioxidant activity obtained IC50 Formula 1 of 157.35 ppm, Formula 2 of 123.40 ppm, Formula 3 of 110.12 ppm, Moringa leaf extract of 58.63 ppm and BHA comparator of 6.84 ppm. Moringa leaf extract and nanoemulsion formula showed good wound healing activity which was faster than the positive control. It was found that Moringa leaf extract and nanoemulsion formula had antibacterial activity against Staphylococcus aureus and Escherichia coli. The wound healing activity of the nanoemulsion formulation was good with the wound healing process in mice faster than the positive control.
THE EFFECT OF MATOA (Pometia pinnata) LEAVES ETHANOL EXTRACT ON REDUCING CHOLESTEROL LEVELS IN MALE MICE(Mus musculus) MuhammadNaufal Syaiful Haq; Halifah Pagarra; Abd Muis; Shopia Salsabila
SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Vol. 14 No. 01 (2025): Volume 14 Nomor 1 (Maret 2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/j72heg19

Abstract

This research aims to determine the total phenolic content and antioxidant activity of the ethanol extract of matoa (Pometia pinnata) leaves and their effect on the cholesterol levels of male mice (Mus musculus). To determine the total phenolic content using the Folin-Ciocalteu method and the DPPH method to measure antioxidant activity. The effect of matoa leaf extract on reducing cholesterol levels in mice used a completely randomized design (CRD) with 15 mice divided into 5 treatment groups with 3 replications, namely P1 (Extract 175 mg/kgBW), P2 (Extract 350 mg/kgBW), P3 (Extract 700 mg/kgBW), K+ (Simvastatin 0.10 mg/kgBW), and K-(Na-CMC 1%). Extraction uses the maceration method with 70% ethanol solvent. The total phenolic test results were 26.24872 mgGAE/g. The results of measuring antioxidant activity obtained an IC50 of 1,961 ppm. The results of testing cholesterol levels showed that there was an effect of giving ethanol extract of Matoa (Pometia pinnata) leaves on reducing cholesterol levels in male mice (Mus musculus). Giving ethanol extract of Matoa leaves at a dose of 350 mg/kgBW is effective in reducing cholesterol levels. So it can be concluded that the ethanol extract of Matoa leaves has an effect on reducing cholesterol levels in male mice
Co-Authors A. Irma Suryani A. Mu&#039;nisa A. Mu&#039;nisa A. Mushawwir Taiyeb A. Mu’nisa Abd Muis Adnan Alimuddin Ali Andi Asmawati Andi Asmawati Asiz Andi Asmawati Azis Andi Bida Purnamasari Andi Faridah Arsal Asham Bin Jamaluddin Asnir Andriani Usman Asraful Ashar Erlin Saalino Erlin Salino Hartati Hartati, Hartati Hartono Hartono Hilda Karim Idris, Irma Suryani Irma Suryani Idris Langi&#039; Khusnul Khatimah Mentari Jelita Putri Muh. Akbar Hidayat Muhammad Ansarullah S. Tabbu Muhammad Ilham Wardhana Haeruddin Muhammad Junda Muhammad Naufal Syaiful Haq MuhammadNaufal Syaiful Haq Musawira Nani Kurnia, Nani Nurul Anisa Oslan Jumadi Oslan Jumadi Patongai, Dian Dwi Putri Ulan Sari Rachmawaty, Rachmawaty Rachmaway Rachmaway Racmawaty Resti Handayani Rosdiana Ngitung Sahribulan Sahribulan Saparuddin Shopia Salsabila Siti Maulyda Ayu MZ St. Fatmah Hiola Sunsun Sahertian Deby Irawan Syamsiah, Syamsiah Syamsul Syamsul Taiyeb A. Mushawwir Theodorus K. Tokan Yasser Abd Djawad Yasser Abd. Djawad Yusminah Hala Zulkifli Maulana